Origin IGS: taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tataaatataaaagcccctgctcggtagtacgagcaagggcaactatgatagaatttgaagttaaaaagagagtgtttatacaggcagatgccctaccctcctttatcggaattatttta Mask Tandem Repeat Region ================================================ taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Find is-nt database================================================ Query_seq: PI0573:PI0574|PI0573:PI0574:aminopeptidase C (bleomycin hydrolase):hypothetical protein:->->:519280..519399 120 taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0573:PI0574|PI0573:PI0574:aminopeptidase C (bleomycin hydrolase):hypothetical protein:->->:519280..519399 120 taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0573:PI0574|PI0573:PI0574:aminopeptidase C (bleomycin hydrolase):hypothetical protein:->->:519280..519399 120 taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 5 End: 118 .... M I P I K E G R A S A C I N T L F L T S N S I I V A L A R T T E Q G L L Y L .. Protein_Len: 30 Strand: + Start: 31 End: 120 .............................. M C L Y K H S L F N F K F Y H S C P C S Y Y R A G A F I F I taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0573:PI0574|PI0573:PI0574:aminopeptidase C (bleomycin hydrolase):hypothetical protein:->->:519280..519399 120 taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Intra-Species Hit: Count: 1 Min: 1 Max: 120 Len: 120 Subject: pint_PI0573_PI0574|aminopeptidase C (bleomycin hydrolase):hypothetical protein|POSITIVE:POSITIVE|[519280,519399]|120 HSP 1 e-value: 3.0E-62 bit: 238.0 Len: 120 Query Start:1 Query End:120 Subject Strand: POSITIVE Subject Start: 1 Subject End: 120 taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata taaaataattccgataaaggagggtagggcatctgcctgtataaacactctctttttaacttcaaattctatcatagttgcccttgctcgtactaccgagcaggggcttttatatttata Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUAAUUCCGAUAAAGGAGGGUAGGGCAUCUGCCUGUAUAAACACUCUCUUUUUAACUUCAAAUUCUAUCAUAGUUGCCCUUGCUCGUACUACCGAGCAGGGGCUUUUAUAUUUAUA ............((.((((((((((.((((....))))........)))))))))).....))..........((((..(((((((((((......)))))))))))..))))....... (-38.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAAAUAUAAAAGCCCCUGCUCGGUAGUACGAGCAAGGGCAACUAUGAUAGAAUUUGAAGUUAAAAAGAGAGUGUUUAUACAGGCAGAUGCCCUACCCUCCUUUAUCGGAAUUAUUUUA (((((((((....((((.((((((......)))))).)))).(((.(((......))).)))..........)))))))))..(((....)))......(((......)))......... (-30.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 110 65 87 34 pint:PI0263|5end_conjugative_transposon_protein,_TraM_255256..255486_POSITIVE 328 108 63 202 163 pint:PI1263|5end_probable_integrase_1184502..1184732_POSITIVE 325 107 67 60 14 pint:PI2121|5end_glucose_inhibited_division_protein_A_2069889..2070119_POSITIVE 323 45 12 111 78 pint:PI0073|5end_dihydropteroate_synthase_70956..71186_POSITIVE 316 114 71 155 96 pint:PI0779|5end_predicted_TPR-repeat-containing_protein_714554..714784_POSITIVE 316 114 71 146 87 pint:PI0778|5end_hypothetical_protein_714545..714775_POSITIVE 305 119 76 64 18 pint:PI1779|5end_16S_rRNA_processing_protein_1711335..1711565_POSITIVE 300 108 61 52 2 pint:PI1171|5end_hypothetical_protein_1091328..1091558_POSITIVE 294 110 66 126 81 pint:PI0727|5end_integrase_659261..659491_POSITIVE 293 56 17 179 139 pint:PI0537|5end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_489756..489986_POSITIVE 290 107 75 94 61 pint:PI0685|5end_elongation_factor_G_620848..621078_POSITIVE 289 107 72 191 161 pint:PI0262|5end_conserved_hypothetical_protein_254685..254915_POSITIVE 287 108 70 75 41 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 282 109 79 217 186 pint:PI0922|5end_conserved_hypothetical_protein_852844..853074_POSITIVE 281 99 54 69 13 pint:PI1098|5end_conserved_hypothetical_protein_1022261..1022491_POSITIVE 279 108 77 218 180 pint:PI0736|5end_transcriptional_regulator,_TetR_family_670580..670810_POSITIVE 275 107 69 139 85 pint:PI1136|5end_conserved_hypothetical_protein_1062195..1062425_POSITIVE 272 103 66 231 195 pint:PI2064|5end_conserved_hypothetical_protein;_possible_type_I_restriction-modification_system,_S_subunit_2025076..2025306_POSITIVE 271 110 66 101 52 pint:PI0580|5end_Holliday_junction_DNA_helicase_524178..524408_POSITIVE 270 120 80 171 127 pint:PI1405|5end_conserved_hypothetical_protein_1326035..1326265_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 110 77 59 20 pint:PI1924|3end_cytidine_deaminase_1866495..1866645_POSITIVE 323 45 12 67 34 pint:PI0072|3end_conserved_hypothetical_protein_70992..71142_POSITIVE 307 110 63 89 42 pint:PI0201|3end_hypothetical_protein_195398..195548_POSITIVE 305 119 76 48 2 pint:PI1778|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1711399..1711549_POSITIVE 298 111 73 39 1 pint:PI0726|3end_hypothetical_protein_659253..659403_POSITIVE 293 56 17 122 82 pint:PI0535|3end_hypothetical_protein_489779..489929_POSITIVE 279 108 77 149 111 pint:PI0735|3end_ABC_transporter,_ATP-binding_/_permease_670591..670741_POSITIVE 277 60 17 150 106 pint:PI2075|3end_conserved_hypothetical_protein_2035355..2035505_POSITIVE 277 111 73 64 25 pint:PI0948|3end_hypothetical_protein_881978..882128_POSITIVE 276 105 72 146 109 pint:PI0033|3end_peptidylprolyl_isomerase_37101..37251_POSITIVE 275 107 69 67 13 pint:PI1135|3end_hypothetical_protein_1062203..1062353_POSITIVE 275 48 10 79 42 pint:PI0377|3end_DNA_primase/mobilizable_transposon,_excision_protein_347823..347973_POSITIVE 271 110 66 52 3 pint:PI0579|3end_hypothetical_protein_524209..524359_POSITIVE 270 120 80 55 11 pint:PI1404|3end_hypothetical_protein_1325999..1326149_POSITIVE 269 120 77 64 8 pint:PI0836|3end_conserved_hypothetical_protein_772373..772523_POSITIVE 267 50 11 99 41 pint:PI0734|3end_ABC_transporter,_ATP-binding_/_permease_668760..668910_POSITIVE 260 116 84 47 17 pint:PI1170|3end_possible_response_regulator_1091398..1091548_POSITIVE 259 108 72 117 75 pint:PI1077|3end_hypothetical_protein_999569..999719_POSITIVE 256 120 83 77 45 pint:PI1506|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_(queuine_synthetase)_1426562..1426712_POSITIVE 255 108 69 96 46 pint:PI0183|3end_hypothetical_protein_174810..174960_POSITIVE