Origin IGS: ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttatgtcagtttgttactgcaaaattacaaaaaagattgtgtttcgtttccctgataaaatattttttcgtatttttgccgtaggaatgtattcattataattatataaaaatcaatcaa Mask Tandem Repeat Region ================================================ ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Find is-nt database================================================ Query_seq: PI0569:PI0570|PI0569:PI0570:phosphoglycerate kinase:exsB protein; possible aluminum resistance protein:->->:514685..514804 120 ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0569:PI0570|PI0569:PI0570:phosphoglycerate kinase:exsB protein; possible aluminum resistance protein:->->:514685..514804 120 ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0569:PI0570|PI0569:PI0570:phosphoglycerate kinase:exsB protein; possible aluminum resistance protein:->->:514685..514804 120 ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 1 End: 93 M I D F Y I I I M N T F L R Q K Y E K I F Y Q G N E T Q S F L ........................... Protein_Len: 32 Strand: - Start: 23 End: 118 ...................... L S Y M G V A F I R F F I K D P F R F V I K K T I K C Y C V S M .. ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0569:PI0570|PI0569:PI0570:phosphoglycerate kinase:exsB protein; possible aluminum resistance protein:->->:514685..514804 120 ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Intra-Species Hit: Count: 1 Min: 1 Max: 120 Len: 120 Subject: pint_PI0569_PI0570|phosphoglycerate kinase:exsB protein; possible aluminum resistance protein|POSITIVE:POSITIVE|[514685,514804]|120 HSP 1 e-value: 3.0E-62 bit: 238.0 Len: 120 Query Start:1 Query End:120 Subject Strand: POSITIVE Subject Start: 1 Subject End: 120 ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa ttgattgatttttatataattataatgaatacattcctacggcaaaaatacgaaaaaatattttatcagggaaacgaaacacaatcttttttgtaattttgcagtaacaaactgacataa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGAUUGAUUUUUAUAUAAUUAUAAUGAAUACAUUCCUACGGCAAAAAUACGAAAAAAUAUUUUAUCAGGGAAACGAAACACAAUCUUUUUUGUAAUUUUGCAGUAACAAACUGACAUAA ..((.(((((((((((....))))).)))).)).)).(((.((((((.(((((((((............(....)...........))))))))).)))))).))).............. (-17.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAUGUCAGUUUGUUACUGCAAAAUUACAAAAAAGAUUGUGUUUCGUUUCCCUGAUAAAAUAUUUUUUCGUAUUUUUGCCGUAGGAAUGUAUUCAUUAUAAUUAUAUAAAAAUCAAUCAA ((((((.((((..((((.((((((.(((.(((((....((((((..((.....))..))))))))))).))).)))))).)))).((((....))))..))))))))))........... (-16.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 240 88 41 182 130 pint:PI0090|5end_amidophosphoribosyltransferase_88379..88609_POSITIVE 207 109 67 45 7 pint:PI0705|5end_conserved_hypothetical_protein;_possible_lipoprotein_641274..641504_POSITIVE 195 120 72 128 84 pint:PI0500|5end_mobilizable_transposon,_tnpC_protein_460674..460904_POSITIVE 194 117 101 229 213 pint:PI0956|5end_conserved_hypothetical_protein_886077..886307_POSITIVE 182 101 64 129 91 pint:PI0857|5end_hypothetical_protein_790697..790927_POSITIVE 182 114 81 193 166 pint:PI0810|5end_hypothetical_protein_746743..746973_POSITIVE 182 114 81 188 161 pint:PI0811|5end_protease/hemagglutinin_PrtT,_streptopain_family_746738..746968_POSITIVE 182 114 81 80 53 pint:PI0809|5end_hypothetical_protein_746630..746860_POSITIVE 180 120 80 84 38 pint:PI2043|5end_hypothetical_protein_1996058..1996288_POSITIVE 178 64 32 164 126 pint:PI0307|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_290042..290272_POSITIVE 177 82 41 46 2 pint:PI1894|5end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino_pimelate_ligase_1836947..1837177_POSITIVE 175 38 3 158 123 pint:PI1148|5end_50S_ribosomal_protein_L9_1074854..1075084_POSITIVE 174 106 75 132 104 pint:PI1817|5end_ISPg3-related_transposase_1755520..1755750_POSITIVE 169 109 67 70 22 pint:PI0066|5end_ABC_transport_system,_ATP-binding_component_65248..65478_POSITIVE 169 77 36 209 167 pint:PI0030|5end_transcriptional_regulator,_AsnC_family_34509..34739_POSITIVE 168 81 42 161 123 pint:PI0419|5end_conserved_hypothetical_protein_386701..386931_POSITIVE 168 81 42 126 88 pint:PI0418|5end_hypothetical_protein_386666..386896_POSITIVE 165 103 61 90 53 pint:PI0988|5end_hypothetical_protein_912347..912577_POSITIVE 164 108 67 95 53 pint:PI0083|5end_tyrosyl-tRNA_synthetase_82824..83054_POSITIVE 163 53 22 47 15 pint:PI1718|5end_DNA_mismatch_repair_protein_1633997..1634227_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 240 88 41 78 26 pint:PI0089|3end_hypothetical_protein_88355..88505_POSITIVE 207 109 67 55 17 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 202 106 84 60 36 pint:PI0013|3end_auxin-regulated_protein_17106..17256_POSITIVE 194 120 88 91 54 pint:PI0544|3end_hypoxanthine-guanine_phosphoribosyltransferase_(hypoxanthine_phosphoribosyltransferase)_496261..496411_POSITIVE 188 70 26 61 12 pint:PI0491|3end_hypothetical_protein_451227..451377_POSITIVE 180 120 80 84 38 pint:PI2042|3end_cation-transporting_ATPase_1996138..1996288_POSITIVE 174 106 75 46 18 pint:PI1816|3end_acid_phosphatase_1755514..1755664_POSITIVE 170 107 61 102 45 pint:PI2123|3end_adenine_phosphoribosyltransferase_2072324..2072474_POSITIVE 169 109 67 54 6 pint:PI0065|3end_conserved_hypothetical_protein_65312..65462_POSITIVE 168 81 42 110 72 pint:PI0417|3end_hypothetical_protein_386730..386880_POSITIVE 168 81 42 87 49 pint:PI0416|3end_hypothetical_protein_386707..386857_POSITIVE 165 63 22 49 6 pint:PI0412|3end_primosomal_protein_N',_replication_factor_Y_382152..382302_POSITIVE 163 95 68 141 106 pint:PI1565|3end_conserved_hypothetical_protein;_possible_surface_antigen_1487357..1487507_POSITIVE 163 84 56 84 60 pint:PI0555|3end_conserved_hypothetical_protein_504974..505124_POSITIVE 162 53 32 37 17 pint:PI0841|3end_hypothetical_protein_775581..775731_POSITIVE 162 120 80 102 64 pint:PI0557|3end_conserved_hypothetical_protein_506884..507034_POSITIVE 161 105 65 85 49 pint:PI2054|3end_hypothetical_protein_2013076..2013226_POSITIVE 160 71 40 46 19 pint:PI0646|3end_hypothetical_protein_587205..587355_POSITIVE 159 92 55 69 22 pint:PI1486|3end_alginate_O-acetylation_protein_1407062..1407212_POSITIVE 158 114 72 66 23 pint:PI1829|3end_hypothetical_protein_1769439..1769589_POSITIVE