Origin IGS: taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagacttttcgttcaagcgaaatagaatattcgataaactaaagaaagaagaaaggaagcccatatcgggtttcctttctttgcttttattccctatctgaacgtgaattcgtcataagaaacacgccagtatattacaaactcacattattctacgaacgaaaatgaggcgataaatatgtaaatatttttcatagtcataatcatcgcttaatcatctaactatcaatacgttacaaaaccta Mask Tandem Repeat Region ================================================ taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Find is-nt database================================================ Query_seq: PI0568:PI0569|PI0568:PI0569:hypothetical protein:phosphoglycerate kinase:->->:513192..513436 245 taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0568:PI0569|PI0568:PI0569:hypothetical protein:phosphoglycerate kinase:->->:513192..513436 245 taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0568:PI0569|PI0568:PI0569:hypothetical protein:phosphoglycerate kinase:->->:513192..513436 245 taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 137 End: 244 ........................................................................................................................................ M H V Q I G N K S K E R K P D M G F L S S F F S L S N I L F R L N E K S . taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 105 End: 134 ........................................................................................................TTTGTAATATACTGGCGTGTTTCTTATGAC............................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -10.8 Tail_Score: -4.65182 Start: 164 End: 198 Full_Region: ATAGGGAATAAAAGC AAAGAAAGGAAACCCG ATA TGGGCTTCCTTTCTTC TTTCTTTAGTTTATC ...................................................................................................................................................................AAAGAAAGGAAACCCGATATGGGCTTCCTTTCTTC............................................... TransTerm Strand: + Conf: 100 HP_score: -12.5 Tail_Score: -4.39724 Start: 167 End: 195 Full_Region: GGGAATAAAAGCAAA GAAAGGAAACCCG ATA TGGGCTTCCTTTC TTCTTTCTTTAGTTT ......................................................................................................................................................................GAAAGGAAACCCGATATGGGCTTCCTTTC.................................................. TransTerm Strand: - Conf: 100 HP_score: -14.0 Tail_Score: -4.3295 Start: 167 End: 195 Full_Region: aaactaaagaaagaa gaaaggaagccc atatc gggtttcctttc tttgcttttattccc ......................................................................................................................................................................gaaaggaagcccatatcgggtttcctttc.................................................. TransTerm Strand: + Conf: 82 HP_score: -7.5 Tail_Score: -4.71584 Start: 171 End: 191 Full_Region: ATAAAAGCAAAGAAA GGAAACCCG ATA TGGGCTTCC TTTCTTCTTTCTTTA ..........................................................................................................................................................................GGAAACCCGATATGGGCTTCC...................................................... TransTerm Strand: - Conf: 90 HP_score: -9.0 Tail_Score: -4.69048 Start: 171 End: 191 Full_Region: taaagaaagaagaaa ggaagccc atatc gggtttcc tttctttgcttttat ..........................................................................................................................................................................ggaagcccatatcgggtttcc...................................................... Find igs database================================================ Query_seq: PI0568:PI0569|PI0568:PI0569:hypothetical protein:phosphoglycerate kinase:->->:513192..513436 245 taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Intra-Species Hit: Count: 1 Min: 1 Max: 245 Len: 245 Subject: pint_PI0568_PI0569|hypothetical protein:phosphoglycerate kinase|POSITIVE:POSITIVE|[513192,513436]|245 HSP 1 e-value: 1.0E-136 bit: 486.0 Len: 245 Query Start:1 Query End:245 Subject Strand: POSITIVE Subject Start: 1 Subject End: 245 taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta taggttttgtaacgtattgatagttagatgattaagcgatgattatgactatgaaaaatatttacatatttatcgcctcattttcgttcgtagaataatgtgagtttgtaatatactggcgtgtttcttatgacgaattcacgttcagatagggaataaaagcaaagaaaggaaacccgatatgggcttcctttcttctttctttagtttatcgaatattctatttcgcttgaacgaaaagtcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUUUGUAACGUAUUGAUAGUUAGAUGAUUAAGCGAUGAUUAUGACUAUGAAAAAUAUUUACAUAUUUAUCGCCUCAUUUUCGUUCGUAGAAUAAUGUGAGUUUGUAAUAUACUGGCGUGUUUCUUAUGACGAAUUCACGUUCAGAUAGGGAAUAAAAGCAAAGAAAGGAAACCCGAUAUGGGCUUCCUUUCUUCUUUCUUUAGUUUAUCGAAUAUUCUAUUUCGCUUGAACGAAAAGUCUA ..((((.(.((((.........)))).).))))..(((((((.((((..(((.....)))....)))).)))))))....(((((((((((((((((..((((((((((.(((.((......))....))).)))))))))).....((((((.((..((((..((((((((((.(((.....))).))))))))))))))..))..))))))...)))))))).......)))))))))..... (-63.11) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGACUUUUCGUUCAAGCGAAAUAGAAUAUUCGAUAAACUAAAGAAAGAAGAAAGGAAGCCCAUAUCGGGUUUCCUUUCUUUGCUUUUAUUCCCUAUCUGAACGUGAAUUCGUCAUAAGAAACACGCCAGUAUAUUACAAACUCACAUUAUUCUACGAACGAAAAUGAGGCGAUAAAUAUGUAAAUAUUUUUCAUAGUCAUAAUCAUCGCUUAAUCAUCUAACUAUCAAUACGUUACAAAACCUA ((((.(((((((((........(((((((...........((((...(((((((((((((((.....))))))))))))))).)))).......((((((..((((..(((.......))).)))).))).)))..............))))))).)))))))))..(((((((.(.((((....(((.....)))..)))).).))))))).....))))........................ (-53.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 511 108 61 130 83 pint:PI0928|5end_hypothetical_protein_859851..860081_POSITIVE 385 78 32 141 95 pint:PI0061|5end_hypothetical_protein_60500..60730_POSITIVE 341 50 1 101 53 pint:PI0104|5end_hypothetical_protein_99105..99335_POSITIVE 332 155 118 155 111 pint:PI0300|5end_transposase_285163..285393_POSITIVE 323 94 51 157 114 pint:PI1733|5end_hypothetical_protein_1652160..1652390_POSITIVE 313 164 124 215 176 pint:PI2110|5end_ISPg3-related_transposase_(IS195_family)_2061470..2061700_POSITIVE 308 50 1 49 1 pint:PI0689|5end_hypothetical_protein_625624..625854_POSITIVE 302 205 161 142 95 pint:PI0848|5end_glycosyl_transferase,_group_2_family_protein_782247..782477_POSITIVE 298 187 143 172 122 pint:PI1537|5end_predicted_TPR-repeat-containing_protein_1455764..1455994_POSITIVE 285 238 200 56 12 pint:PI0030|5end_transcriptional_regulator,_AsnC_family_34509..34739_POSITIVE 284 50 1 124 75 pint:PI1195|5end_hypothetical_protein_1113171..1113401_POSITIVE 281 50 1 189 141 pint:PI0157|5end_hypothetical_protein_151185..151415_POSITIVE 280 43 1 95 49 pint:PI1905|5end_hypothetical_protein_1847628..1847858_POSITIVE 279 203 171 63 23 pint:PI1653|5end_hypothetical_protein_1565158..1565388_POSITIVE 275 88 52 161 126 pint:PI0755|5end_hypothetical_protein_686691..686921_POSITIVE 274 118 73 98 55 pint:PI1747|5end_membrane-bound_lytic_murein_transglycosylase_D_1667483..1667713_POSITIVE 274 43 1 114 75 pint:PI1666|5end_conserved_hypothetical_protein_1579231..1579461_POSITIVE 270 198 152 80 22 pint:PI0929|5end_copper_homeostasis_protein_860294..860524_POSITIVE 266 187 142 135 84 pint:PI1189|5end_conserved_hypothetical_protein_1107114..1107344_POSITIVE 265 187 146 176 127 pint:PI0641|5end_conserved_hypothetical_protein_583791..584021_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 308 50 1 95 47 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 306 48 1 94 41 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 298 187 143 136 86 pint:PI1536|3end_hypothetical_protein_1455808..1455958_POSITIVE 283 205 162 47 1 pint:PI0847|3end_thiamine_biosynthesis_lipoprotein,_ApbE_782232..782382_POSITIVE 282 69 36 36 3 pint:PI0103|3end_conserved_hypothetical_protein;_possible_permease_99100..99250_POSITIVE 279 203 171 60 20 pint:PI1652|3end_hypothetical_protein_1565235..1565385_POSITIVE 274 118 73 141 98 pint:PI1746|3end_hypothetical_protein_1667606..1667756_POSITIVE 271 73 32 61 20 pint:PI2172|3end_hypothetical_protein_2113991..2114141_POSITIVE 265 187 146 126 77 pint:PI0640|3end_conserved_hypothetical_protein_583821..583971_POSITIVE 262 185 141 79 26 pint:PI1885|3end_cell_division_protein_1827639..1827789_POSITIVE 262 214 178 62 19 pint:PI1636|3end_hypothetical_protein_1554823..1554973_POSITIVE 262 76 32 48 4 pint:PI0770|3end_hypothetical_protein_706354..706504_POSITIVE 262 214 178 62 19 pint:PI0341|3end_conserved_hypothetical_protein_317615..317765_POSITIVE 260 155 119 85 50 pint:PI1015|3end_conserved_hypothetical_protein_937347..937497_POSITIVE 259 57 13 101 54 pint:PI1199|3end_possible_acetyltransferase_1115746..1115896_POSITIVE 257 187 141 144 97 pint:PI0130|3end_hypothetical_protein_122267..122417_POSITIVE 255 215 182 122 91 pint:PI0513|3end_ion_transporter_472371..472521_POSITIVE 252 230 183 102 49 pint:PI1586|3end_possible_HesA/MoeB/ThiF_family_protein_1515183..1515333_POSITIVE 251 107 69 74 41 pint:PI1049|3end_hypothetical_protein_967341..967491_POSITIVE 251 176 132 85 46 pint:PI0804|3end_conserved_hypothetical_protein;_possible_membrane_protein_742790..742940_POSITIVE