Origin IGS: taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatctccgcactccaaaacacataaaacgacacggggcgacacgcatagtgccgccccgtgttgttttatcttccttataagtatcgccattacgatataaaacaaccacacaattgcttataaattataacttacgcatctacctaataccttaaaaacctattttaaatacctttttcattgatta Mask Tandem Repeat Region ================================================ taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Find is-nt database================================================ Query_seq: PI0564:PI0566|PI0564:PI0566:hypothetical protein:enoyl-[acyl-carrier-protein] reductase:->->:511782..511971 190 taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0564:PI0566|PI0564:PI0566:hypothetical protein:enoyl-[acyl-carrier-protein] reductase:->->:511782..511971 190 taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0564:PI0566|PI0564:PI0566:hypothetical protein:enoyl-[acyl-carrier-protein] reductase:->->:511782..511971 190 taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: + Start: 67 End: 189 .................................................................. M S N C V V V L Y R N G D T Y K E D K T T R G G T M R V A P C R F M C F G V R R F . Protein_Len: 39 Strand: + Start: 74 End: 190 ......................................................................... M V W L F Y I V M A I L I R K I K Q H G A A L C V S P R V V L C V L E C G D L taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -22.1 Tail_Score: -3.6692 Start: 118 End: 168 Full_Region: GATACTTATAAGGAA GATAAAACAACACGGGGCGGCAC TATGC GTGTCGCCCCGTGTCGTTTTATG TGTTTTGGAGTGCGG .....................................................................................................................GATAAAACAACACGGGGCGGCACTATGCGTGTCGCCCCGTGTCGTTTTATG...................... TransTerm Strand: - Conf: 100 HP_score: -22.1 Tail_Score: -3.46659 Start: 118 End: 168 Full_Region: ccgcactccaaaaca cataaaacgacacggggcgacac gcata gtgccgccccgtgttgttttatc ttccttataagtatc .....................................................................................................................cataaaacgacacggggcgacacgcatagtgccgccccgtgttgttttatc...................... TransTerm Strand: + Conf: 100 HP_score: -24.7 Tail_Score: -3.41366 Start: 120 End: 166 Full_Region: TACTTATAAGGAAGA TAAAACAACACGGGGCGGCAC TATGC GTGTCGCCCCGTGTCGTTTTA TGTGTTTTGGAGTGC .......................................................................................................................TAAAACAACACGGGGCGGCACTATGCGTGTCGCCCCGTGTCGTTTTA........................ TransTerm Strand: - Conf: 100 HP_score: -24.7 Tail_Score: -3.30039 Start: 120 End: 166 Full_Region: gcactccaaaacaca taaaacgacacggggcgacac gcata gtgccgccccgtgttgtttta tcttccttataagta .......................................................................................................................taaaacgacacggggcgacacgcatagtgccgccccgtgttgtttta........................ TransTerm Strand: + Conf: 100 HP_score: -20.2 Tail_Score: -4.78933 Start: 125 End: 161 Full_Region: ATAAGGAAGATAAAA CAACACGGGGCGGCAC TATGC GTGTCGCCCCGTGTCG TTTTATGTGTTTTGG ............................................................................................................................CAACACGGGGCGGCACTATGCGTGTCGCCCCGTGTCG............................. TransTerm Strand: - Conf: 100 HP_score: -20.2 Tail_Score: -4.74767 Start: 125 End: 161 Full_Region: ccaaaacacataaaa cgacacggggcgacac gcata gtgccgccccgtgttg ttttatcttccttat ............................................................................................................................cgacacggggcgacacgcatagtgccgccccgtgttg............................. Find igs database================================================ Query_seq: PI0564:PI0566|PI0564:PI0566:hypothetical protein:enoyl-[acyl-carrier-protein] reductase:->->:511782..511971 190 taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Intra-Species Hit: Count: 1 Min: 1 Max: 190 Len: 190 Subject: pint_PI0564_PI0566|hypothetical protein:enoyl-[acyl-carrier-protein] reductase|POSITIVE:POSITIVE|[511782,511971]|190 HSP 1 e-value: 1.0E-104 bit: 377.0 Len: 190 Query Start:1 Query End:190 Subject Strand: POSITIVE Subject Start: 1 Subject End: 190 taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta taatcaatgaaaaaggtatttaaaataggtttttaaggtattaggtagatgcgtaagttataatttataagcaattgtgtggttgttttatatcgtaatggcgatacttataaggaagataaaacaacacggggcggcactatgcgtgtcgccccgtgtcgttttatgtgttttggagtgcggagattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCAAUGAAAAAGGUAUUUAAAAUAGGUUUUUAAGGUAUUAGGUAGAUGCGUAAGUUAUAAUUUAUAAGCAAUUGUGUGGUUGUUUUAUAUCGUAAUGGCGAUACUUAUAAGGAAGAUAAAACAACACGGGGCGGCACUAUGCGUGUCGCCCCGUGUCGUUUUAUGUGUUUUGGAGUGCGGAGAUUUA ...............((((((((((((..(((((((((((((.(.((..((((......(((((((((((....))))).)))))).......)))).)).)))))))).))))))..(((((((.((((((((((((((.....)))))))))))))).))))))).)))))).))))))......... (-56.42) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUCUCCGCACUCCAAAACACAUAAAACGACACGGGGCGACACGCAUAGUGCCGCCCCGUGUUGUUUUAUCUUCCUUAUAAGUAUCGCCAUUACGAUAUAAAACAACCACACAAUUGCUUAUAAAUUAUAACUUACGCAUCUACCUAAUACCUUAAAAACCUAUUUUAAAUACCUUUUUCAUUGAUUA ..((((...((............((((((((((((((((((.(((.....))).)))))))))))))))))).....((((((((((((......))))...................))))))))............))..............((((((.....))))))..............)))). (-44.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 439 188 142 83 37 pint:PI1994|5end_hypothetical_protein_1949360..1949590_POSITIVE 426 161 125 41 5 pint:PI1647|5end_hypothetical_protein_1561564..1561794_POSITIVE 408 186 143 110 70 pint:PI1711|5end_ABC_transporter,_ATP-binding_protein_1627597..1627827_POSITIVE 390 180 134 62 4 pint:PI0336|5end_hypothetical_protein_314499..314729_POSITIVE 386 163 124 90 45 pint:PI0768|5end_conserved_hypothetical_protein;_possible_acetyltransferase_704072..704302_POSITIVE 379 185 141 219 164 pint:PI0596|5end_hypothetical_protein_546400..546630_POSITIVE 367 164 132 101 59 pint:PI1550|5end_conserved_hypothetical_protein_1467360..1467590_POSITIVE 358 179 134 83 38 pint:PI1826|5end_ATP-dependent_DNA_helicase_1765650..1765880_POSITIVE 355 162 131 32 2 pint:PI2017|5end_conserved_hypothetical_protein_1974862..1975092_POSITIVE 352 180 133 105 56 pint:PI0260|5end_conjugative_transposon_protein,_TraJ_253023..253253_POSITIVE 350 179 132 88 35 pint:PI1364|5end_dipeptidyl_peptidase_IV_1281862..1282092_POSITIVE 344 152 113 82 43 pint:PI0668|5end_peptidyl-prolyl_cis-trans_isomerase_SlyD,_FKBP-type_(peptidylprolyl_isomerase)_610216..610446_POSITIVE 341 180 134 66 27 pint:PI1692|5end_alanine_racemase_1605691..1605921_POSITIVE 333 180 135 128 82 pint:PI0599|5end_hypothetical_protein_547376..547606_POSITIVE 331 169 127 221 178 pint:PI1328|5end_conserved_hypothetical_protein_1246197..1246427_POSITIVE 329 165 128 213 178 pint:PI2134|5end_hypothetical_protein_2082269..2082499_POSITIVE 327 159 129 227 197 pint:PI0754|5end_possible_outer_membrane_receptor_683771..684001_POSITIVE 325 187 147 224 174 pint:PI1166|5end_hypothetical_protein_1089305..1089535_POSITIVE 325 163 132 185 155 pint:PI0224|5end_conserved_hypothetical_protein_220541..220771_POSITIVE 320 189 143 69 3 pint:PI1859|5end_conserved_hypothetical_protein_1805394..1805624_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 379 185 141 145 90 pint:PI0595|3end_conserved_hypothetical_protein_546406..546556_POSITIVE 375 180 131 134 76 pint:PI0421|3end_hypothetical_protein_387774..387924_POSITIVE 367 164 132 43 1 pint:PI1549|3end_conserved_hypothetical_protein_1467382..1467532_POSITIVE 364 177 131 142 82 pint:PI0639|3end_small_heat_shock_protein_583165..583315_POSITIVE 358 179 134 58 13 pint:PI1825|3end_2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase_1765705..1765855_POSITIVE 355 180 131 77 30 pint:PI1710|3end_ABC-type_permease_component_1627356..1627506_POSITIVE 350 179 132 68 15 pint:PI1363|3end_hypothetical_protein_1281922..1282072_POSITIVE 348 165 131 140 108 pint:PI2075|3end_conserved_hypothetical_protein_2035355..2035505_POSITIVE 336 174 131 78 36 pint:PI0587|3end_conserved_hypothetical_protein_535347..535497_POSITIVE 334 176 135 147 96 pint:PI1623|3end_hypothetical_protein_1549261..1549411_POSITIVE 328 165 125 147 108 pint:PI0588|3end_hypothetical_protein_535425..535575_POSITIVE 325 163 132 147 117 pint:PI0223|3end_topoisomerase_IA_220583..220733_POSITIVE 324 180 131 69 13 pint:PI0685|3end_elongation_factor_G_623048..623198_POSITIVE 323 157 112 70 23 pint:PI1599|3end_predicted_TPR-repeat-containing_protein_1529176..1529326_POSITIVE 322 170 130 48 5 pint:PI0053|3end_hypothetical_protein_55124..55274_POSITIVE 319 172 131 143 97 pint:PI1608|3end_possible_Na+/H+_anti-porter_1536300..1536450_POSITIVE 316 176 136 146 101 pint:PI0113|3end_arginyl-tRNA_synthetase_110082..110232_POSITIVE 309 179 131 111 41 pint:PI0839|3end_conserved_hypothetical_protein_774210..774360_POSITIVE 306 161 127 55 11 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 302 170 130 107 48 pint:PI1716|3end_hypothetical_protein_1632006..1632156_POSITIVE