Origin IGS: taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaaacaagaccaatgaaacgatagtcttcttcattttctgttttatttaaggtgtttttagtattctctaattaagtggaaaagaaaggcggaagcctctccaagaaggttccgccgtttgtattgattgcttccgaggatta Mask Tandem Repeat Region ================================================ taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Find is-nt database================================================ Query_seq: PI0547:PI0548|PI0547:PI0548:fructose-bisphosphate aldolase:possible acetylxylan esterase:->->:499527..499671 145 taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0547:PI0548|PI0547:PI0548:fructose-bisphosphate aldolase:possible acetylxylan esterase:->->:499527..499671 145 taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0547:PI0548|PI0547:PI0548:fructose-bisphosphate aldolase:possible acetylxylan esterase:->->:499527..499671 145 taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Predict ORF larger than 30AA ================================================ taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 83 HP_score: -8.7 Tail_Score: -4.21768 Start: 24 End: 60 Full_Region: GGAAGCAATCAATAC AAACGGCGGAACCTTCT TGG AGAGGCTTCCGCCTTTC TTTTCCACTTAATTA .......................AAACGGCGGAACCTTCTTGGAGAGGCTTCCGCCTTTC..................................................................................... TransTerm Strand: - Conf: 82 HP_score: -8.2 Tail_Score: -4.28323 Start: 27 End: 57 Full_Region: ttaagtggaaaagaa aggcggaagcctct cca agaaggttccgccg tttgtattgattgct ..........................aggcggaagcctctccaagaaggttccgccg........................................................................................ TransTerm Strand: - Conf: 83 HP_score: -11.7 Tail_Score: -3.15909 Start: 28 End: 56 Full_Region: taagtggaaaagaaa ggcggaagcctct cca agaaggttccgcc gtttgtattgattgc ...........................ggcggaagcctctccaagaaggttccgcc......................................................................................... Find igs database================================================ Query_seq: PI0547:PI0548|PI0547:PI0548:fructose-bisphosphate aldolase:possible acetylxylan esterase:->->:499527..499671 145 taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Intra-Species Hit: Count: 1 Min: 1 Max: 145 Len: 145 Subject: pint_PI0547_PI0548|fructose-bisphosphate aldolase:possible acetylxylan esterase|POSITIVE:POSITIVE|[499527,499671]|145 HSP 1 e-value: 5.0E-77 bit: 287.0 Len: 145 Query Start:1 Query End:145 Subject Strand: POSITIVE Subject Start: 1 Subject End: 145 taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta taatcctcggaagcaatcaatacaaacggcggaaccttcttggagaggcttccgcctttcttttccacttaattagagaatactaaaaacaccttaaataaaacagaaaatgaagaagactatcgtttcattggtcttgtttcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCUCGGAAGCAAUCAAUACAAACGGCGGAACCUUCUUGGAGAGGCUUCCGCCUUUCUUUUCCACUUAAUUAGAGAAUACUAAAAACACCUUAAAUAAAACAGAAAAUGAAGAAGACUAUCGUUUCAUUGGUCUUGUUUCUA ........(((((((((((((..(((((..((...((((((.(((((((....)))))))............((((......))))..........................)))))).))..))))).))))))..))))))). (-32.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAACAAGACCAAUGAAACGAUAGUCUUCUUCAUUUUCUGUUUUAUUUAAGGUGUUUUUAGUAUUCUCUAAUUAAGUGGAAAAGAAAGGCGGAAGCCUCUCCAAGAAGGUUCCGCCGUUUGUAUUGAUUGCUUCCGAGGAUUA ((((((((.....((((((((((.(((.......))).)).)))))))).....))))))))..((((((......(((.((...(((.(((((((.((((.....).)))))))))).)))...)).)))......)))))).. (-33.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 366 58 12 132 83 pint:PI0916|5end_hypothetical_protein_845617..845847_POSITIVE 357 59 28 62 19 pint:PI0213|5end_conserved_hypothetical_protein_207065..207295_POSITIVE 343 60 27 187 144 pint:PI0583|5end_hypothetical_protein_525948..526178_POSITIVE 335 58 13 82 27 pint:PI1705|5end_3-oxoacyl-(acyl-carrier-protein)_synthase_III_1621781..1622011_POSITIVE 328 62 27 33 4 pint:PI0258|5end_conjugative_transposon_protein,_TraG_249722..249952_POSITIVE 308 56 11 186 119 pint:PI0784|5end_possible_amidase_enhancer_precursor_721892..722122_POSITIVE 304 62 27 60 33 pint:PI0262|5end_conserved_hypothetical_protein_254685..254915_POSITIVE 294 55 11 122 58 pint:PI2166|5end_hypothetical_protein_2111394..2111624_POSITIVE 291 59 25 215 182 pint:PI0432|5end_hypothetical_protein_404781..405011_POSITIVE 291 59 25 158 125 pint:PI0433|5end_hypothetical_protein_404724..404954_POSITIVE 291 70 27 186 136 pint:PI0331|5end_hypothetical_protein_311656..311886_POSITIVE 288 70 28 162 125 pint:PI0516|5end_ferredoxin,_4Fe-4S_475864..476094_POSITIVE 284 67 27 126 84 pint:PI0068|5end_arginine_repressor_66586..66816_POSITIVE 282 70 28 81 22 pint:PI1653|5end_hypothetical_protein_1565158..1565388_POSITIVE 281 65 28 79 41 pint:PI0744|5end_DNA_primase/mobilizable_transposon,_excision_protein_674171..674401_POSITIVE 280 59 12 213 161 pint:PI0447|5end_DNA-directed_RNA_polymerase,_beta'_subunit_417314..417544_POSITIVE 277 57 12 52 8 pint:PI0261|5end_conjugative_transposon_protein,_TraK_254065..254295_POSITIVE 275 54 13 212 161 pint:PI0252|5end_conserved_hypothetical_protein_247761..247991_POSITIVE 274 48 4 44 4 pint:PI1955|5end_N_utilization_substance_protein_A_1911782..1912012_POSITIVE 273 68 26 69 23 pint:PI0404|5end_integrase_371295..371525_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 328 62 27 33 4 pint:PI0257|3end_conjugative_transposon_protein,_TraF_249802..249952_POSITIVE 323 47 9 150 114 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 311 56 28 63 35 pint:PI0547|3end_fructose-bisphosphate_aldolase_499466..499616_POSITIVE 308 56 11 141 74 pint:PI0782|3end_beta-lactamase_induction_signal_transducer,_AmpG_protein_721927..722077_POSITIVE 304 62 27 60 33 pint:PI0261|3end_conjugative_transposon_protein,_TraK_254765..254915_POSITIVE 294 49 4 104 46 pint:PI0315|3end_conserved_hypothetical_protein_298186..298336_POSITIVE 292 79 38 124 83 pint:PI0513|3end_ion_transporter_472371..472521_POSITIVE 290 49 23 73 46 pint:PI1954|3end_translation_initiation_factor_IF-2_1911647..1911797_POSITIVE 284 67 27 46 4 pint:PI0067|3end_hypothetical_protein_66586..66736_POSITIVE 282 70 28 78 19 pint:PI1652|3end_hypothetical_protein_1565235..1565385_POSITIVE 270 62 28 53 23 pint:PI1828|3end_peptidase,_M23/M37_family,_C-terminal_1769089..1769239_POSITIVE 264 65 26 57 3 pint:PI0358|3end_hypothetical_protein_328105..328255_POSITIVE 262 68 28 150 94 pint:PI0683|3end_hypothetical_protein_619797..619947_POSITIVE 262 58 12 140 87 pint:PI0114|3end_hypothetical_protein_108527..108677_POSITIVE 261 70 23 142 84 pint:PI1548|3end_conserved_hypothetical_protein_1466886..1467036_POSITIVE 259 60 13 141 85 pint:PI1651|3end_hypothetical_protein_1564512..1564662_POSITIVE 259 69 24 81 28 pint:PI0928|3end_hypothetical_protein_860272..860422_POSITIVE 258 70 26 86 36 pint:PI0612|3end_lysyl-tRNA_synthetase_562202..562352_POSITIVE 253 70 27 49 4 pint:PI0670|3end_hypothetical_protein_611280..611430_POSITIVE 253 49 3 67 20 pint:PI0347|3end_hypothetical_protein_321655..321805_POSITIVE