Origin IGS: tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatggaaaaccgttattcacctttggtattgattaacacgataagtcatttgcaaatatagctatttttcttgtattagaggctaaaagacttccttttttctatatgtcttgtca Mask Tandem Repeat Region ================================================ tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Find is-nt database================================================ Query_seq: PI0513:PI0514|PI0513:PI0514:ion transporter:possible cell filamentation protein:->->:472432..472547 116 tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0513:PI0514|PI0513:PI0514:ion transporter:possible cell filamentation protein:->->:472432..472547 116 tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0513:PI0514|PI0513:PI0514:ion transporter:possible cell filamentation protein:->->:472432..472547 116 tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Predict ORF larger than 30AA ================================================ tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0513:PI0514|PI0513:PI0514:ion transporter:possible cell filamentation protein:->->:472432..472547 116 tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Intra-Species Hit: Count: 1 Min: 1 Max: 116 Len: 116 Subject: pint_PI0513_PI0514|ion transporter:possible cell filamentation protein|POSITIVE:POSITIVE|[472432,472547]|116 HSP 1 e-value: 8.0E-60 bit: 230.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt tgacaagacatatagaaaaaaggaagtcttttagcctctaatacaagaaaaatagctatatttgcaaatgacttatcgtgttaatcaataccaaaggtgaataacggttttccatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACAAGACAUAUAGAAAAAAGGAAGUCUUUUAGCCUCUAAUACAAGAAAAAUAGCUAUAUUUGCAAAUGACUUAUCGUGUUAAUCAAUACCAAAGGUGAAUAACGGUUUUCCAUU .....................(((((.((.(((((((.................((.......))............(((((....)))))...))))...))).)).)))))... (-12.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGGAAAACCGUUAUUCACCUUUGGUAUUGAUUAACACGAUAAGUCAUUUGCAAAUAUAGCUAUUUUUCUUGUAUUAGAGGCUAAAAGACUUCCUUUUUUCUAUAUGUCUUGUCA .((((((((..((((.(((((...)))...)).)))).....(((((.(((((.(((((((.........)))))))....)).))).)))))....))))))))........... (-16.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 295 107 63 110 61 pint:PI0945|5end_sodium:solute_symporter_family_protein_876249..876479_POSITIVE 247 100 51 201 150 pint:PI1818|5end_hypothetical_protein_1756891..1757121_POSITIVE 234 108 64 111 65 pint:PI0640|5end_conserved_hypothetical_protein_583289..583519_POSITIVE 222 108 62 61 1 pint:PI0855|5end_hypothetical_protein_789094..789324_POSITIVE 218 99 59 143 103 pint:PI1846|5end_hypothetical_protein_1791705..1791935_POSITIVE 214 108 61 84 36 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 210 93 51 156 119 pint:PI0350|5end_conserved_hypothetical_protein_323757..323987_POSITIVE 209 91 55 228 192 pint:PI2035|5end_Holliday_junction_DNA_helicase_ruvB_1988212..1988442_POSITIVE 209 100 51 201 156 pint:PI0418|5end_hypothetical_protein_386666..386896_POSITIVE 209 100 51 86 41 pint:PI0420|5end_hypothetical_protein_386551..386781_POSITIVE 209 100 51 72 27 pint:PI0417|5end_hypothetical_protein_386537..386767_POSITIVE 208 100 55 214 181 pint:PI0953|5end_hypothetical_protein_885119..885349_POSITIVE 205 110 61 191 151 pint:PI0561|5end_hypothetical_protein_508952..509182_POSITIVE 202 80 34 159 111 pint:PI1069|5end_hypothetical_protein_988067..988297_POSITIVE 201 70 21 146 96 pint:PI1823|5end_inorganic_polyphosphate/ATP-NAD_kinase_(Poly(P)/ATP_NAD_kinase)_1763238..1763468_POSITIVE 201 110 62 163 124 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 197 99 55 69 25 pint:PI0867|5end_hypothetical_protein_803859..804089_POSITIVE 196 110 62 98 59 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 196 97 51 230 191 pint:PI0419|5end_conserved_hypothetical_protein_386701..386931_POSITIVE 191 100 63 147 114 pint:PI0319|5end_conserved_hypothetical_protein_301750..301980_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 221 95 55 63 20 pint:PI2034|3end_conserved_hypothetical_protein_1988120..1988270_POSITIVE 218 99 59 110 70 pint:PI1845|3end_hypothetical_protein_1791752..1791902_POSITIVE 218 99 59 110 70 pint:PI1839|3end_hypothetical_protein_1782500..1782650_POSITIVE 214 108 61 138 90 pint:PI0118|3end_hypothetical_protein_111640..111790_POSITIVE 210 93 51 121 84 pint:PI0349|3end_hypothetical_protein_323802..323952_POSITIVE 209 41 1 70 25 pint:PI1444|3end_protease_ClpB_1368137..1368287_POSITIVE 207 99 62 150 118 pint:PI1064|3end_hypothetical_protein_983324..983474_POSITIVE 205 110 61 69 29 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 204 81 41 127 86 pint:PI1280|3end_conserved_hypothetical_protein_1196554..1196704_POSITIVE 201 70 21 93 43 pint:PI1822|3end_ABC_transporter,_ATP-binding_/_permease_1763265..1763415_POSITIVE 197 99 55 123 79 pint:PI0866|3end_hypothetical_protein_803993..804143_POSITIVE 195 108 61 146 101 pint:PI0141|3end_glutamine_amidotransferase_involved_in_pyridoxine_biosynthesis_133308..133458_POSITIVE 194 97 58 74 34 pint:PI1424|3end_probable_peptidase_family_M48_1344044..1344194_POSITIVE 192 99 60 150 116 pint:PI1204|3end_hypothetical_protein_1124635..1124785_POSITIVE 191 100 63 125 92 pint:PI0318|3end_conserved_hypothetical_protein_301808..301958_POSITIVE 189 99 55 123 88 pint:PI0820|3end_hypothetical_protein_759980..760130_POSITIVE 189 99 55 62 27 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 189 62 26 68 32 pint:PI0632|3end_NusB_family_protein_(N_utilization_substance_protein_B)_579731..579881_POSITIVE 188 65 30 110 70 pint:PI2103|3end_hypothetical_protein_2057934..2058084_POSITIVE 185 70 21 82 41 pint:PI0575|3end_hypothetical_protein_520228..520378_POSITIVE