Origin IGS: taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttataagttttattatttattcttacggttcttcagccatttgtttatcattctcatgatgatcatacatccttgctgattttatcagataattctcttgtcattatccgtccattgctcacattgtttgaaaacagtttctaatgcgtaggcaactgcttcttgtggatagtgtacttcttgagtaatcgctta Mask Tandem Repeat Region ================================================ taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Find is-nt database================================================ Query_seq: PI0508:PI0509|PI0508:PI0509:possible cell filamentation protein:conserved hypothetical protein:->->:467497..467693 197 taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0508:PI0509|PI0508:PI0509:possible cell filamentation protein:conserved hypothetical protein:->->:467497..467693 197 taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0508:PI0509|PI0508:PI0509:possible cell filamentation protein:conserved hypothetical protein:->->:467497..467693 197 taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 7 End: 108 ...... M T Q E V H Y P Q E A V A Y A L E T V F K Q C E Q W T D N D K R I I ......................................................................................... Protein_Len: 41 Strand: - Start: 55 End: 177 ...................................................... F S N E F L T L L P R I I V L S N D S L I L L S T H D D H S H Y V F P Q L V T L M .................... taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0508:PI0509|PI0508:PI0509:possible cell filamentation protein:conserved hypothetical protein:->->:467497..467693 197 taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Intra-Species Hit: Count: 1 Min: 1 Max: 197 Len: 197 Subject: pint_PI0508_PI0509|possible cell filamentation protein:conserved hypothetical protein|POSITIVE:POSITIVE|[467497,467693]|197 HSP 1 e-value: 1.0E-108 bit: 391.0 Len: 197 Query Start:1 Query End:197 Subject Strand: POSITIVE Subject Start: 1 Subject End: 197 taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat taagcgattactcaagaagtacactatccacaagaagcagttgcctacgcattagaaactgttttcaaacaatgtgagcaatggacggataatgacaagagaattatctgataaaatcagcaaggatgtatgatcatcatgagaatgataaacaaatggctgaagaaccgtaagaataaataataaaacttataaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCGAUUACUCAAGAAGUACACUAUCCACAAGAAGCAGUUGCCUACGCAUUAGAAACUGUUUUCAAACAAUGUGAGCAAUGGACGGAUAAUGACAAGAGAAUUAUCUGAUAAAAUCAGCAAGGAUGUAUGAUCAUCAUGAGAAUGAUAAACAAAUGGCUGAAGAACCGUAAGAAUAAAUAAUAAAACUUAUAAAU .......(((.((.....((.(((.........(((((((((..(((.....))).)))))))))........))).)).((((.((((((((.........))))))))......(((((.....(((...((((((....).)))))..)))....)))))....))))..)).))).................. (-34.73) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUAUAAGUUUUAUUAUUUAUUCUUACGGUUCUUCAGCCAUUUGUUUAUCAUUCUCAUGAUGAUCAUACAUCCUUGCUGAUUUUAUCAGAUAAUUCUCUUGUCAUUAUCCGUCCAUUGCUCACAUUGUUUGAAAACAGUUUCUAAUGCGUAGGCAACUGCUUCUUGUGGAUAGUGUACUUCUUGAGUAAUCGCUUA .....(((((...((((((((.......(((...(((((....(((..((((((.....))))))...))).....)))))....))).............(((((((((((..(((((.....((((((....))))))...))))).((((....))))......))))))))).))....)))))))).))))) (-39.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 305 49 4 58 16 pint:PI1334|5end_ferrodoxin_1251404..1251634_POSITIVE 301 51 12 198 151 pint:PI0920|5end_xanthan_lyase_849688..849918_POSITIVE 293 60 16 122 72 pint:PI1882|5end_hypothetical_protein_1825787..1826017_POSITIVE 290 79 37 209 168 pint:PI1700|5end_hypothetical_protein_1617607..1617837_POSITIVE 286 50 1 148 92 pint:PI1232|5end_conserved_hypothetical_protein_1152757..1152987_POSITIVE 283 50 5 128 89 pint:PI1575|5end_LemA_protein_1501143..1501373_POSITIVE 281 50 2 113 59 pint:PI0707|5end_conserved_hypothetical_protein;_possible_integral_membrane_protein_643358..643588_POSITIVE 277 54 12 41 1 pint:PI1551|5end_HesA/MoeB/ThiF_family_protein_1467787..1468017_POSITIVE 272 45 3 87 40 pint:PI1032|5end_methylmalonyl-CoA_decarboxylase,_alpha_subunit_948463..948693_POSITIVE 269 56 25 200 168 pint:PI0244|5end_possible_mobilization_protein_C_240752..240982_POSITIVE 264 87 41 52 3 pint:PI0577|5end_conserved_hypothetical_protein_521811..522041_POSITIVE 260 79 33 75 26 pint:PI0568|5end_hypothetical_protein_512950..513180_POSITIVE 259 70 23 119 66 pint:PI1946|5end_hypothetical_protein_1902713..1902943_POSITIVE 256 50 4 179 128 pint:PI0668|5end_peptidyl-prolyl_cis-trans_isomerase_SlyD,_FKBP-type_(peptidylprolyl_isomerase)_610216..610446_POSITIVE 256 50 4 76 25 pint:PI0667|5end_hypothetical_protein_610113..610343_POSITIVE 252 80 36 57 8 pint:PI0586|5end_hypothetical_protein_533757..533987_POSITIVE 251 50 4 45 4 pint:PI0817|5end_glycosyl_transferase,_group_1_family_protein_756861..757091_POSITIVE 250 86 54 179 147 pint:PI1537|5end_predicted_TPR-repeat-containing_protein_1455764..1455994_POSITIVE 248 166 123 227 191 pint:PI1236|5end_10_kDa_chaperonin,_cpn10,_GroES_1158934..1159164_POSITIVE 246 49 14 152 115 pint:PI0282|5end_hypothetical_protein_266540..266770_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 51 12 149 102 pint:PI0919|3end_conserved_hypothetical_protein_849719..849869_POSITIVE 283 50 5 131 92 pint:PI1574|3end_conserved_hypothetical_protein_1501226..1501376_POSITIVE 281 50 2 69 15 pint:PI0706|3end_pyridoxal_phosphate_biosynthetic_protein_(4-hydroxythreonine-4-phosphate_dehydrogenase)_643394..643544_POSITIVE 272 45 3 51 4 pint:PI1031|3end_hypothetical_protein_948507..948657_POSITIVE 269 56 25 106 74 pint:PI0243|3end_hypothetical_protein_240738..240888_POSITIVE 269 54 15 74 22 pint:PI0027|3end_aminopeptidase_C_31756..31906_POSITIVE 260 79 33 100 51 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE 259 51 14 124 82 pint:PI2099|3end_D-alanyl-D-alanine_dipeptidase_2053277..2053427_POSITIVE 256 50 4 54 3 pint:PI0666|3end_chorismate_synthase_610171..610321_POSITIVE 250 86 54 143 111 pint:PI1536|3end_hypothetical_protein_1455808..1455958_POSITIVE 250 50 4 136 76 pint:PI0797|3end_tRNA_pseudouridine_synthase_A_(pseudouridylate_synthase)_735752..735902_POSITIVE 248 166 123 101 65 pint:PI1235|3end_60_kDa_chaperonin,_groEL,_cpn60_1158888..1159038_POSITIVE 246 49 14 136 99 pint:PI0281|3end_conserved_hypothetical_protein_266604..266754_POSITIVE 245 67 28 56 19 pint:PI0355|3end_probable__N-acetylmuramoyl-L-alanine_amidase_326698..326848_POSITIVE 243 100 55 48 7 pint:PI0226|3end_ABC_transporter,_ATP-binding_/_permease_protein_223884..224034_POSITIVE 242 50 3 142 87 pint:PI0114|3end_hypothetical_protein_108527..108677_POSITIVE 237 56 14 111 59 pint:PI2032|3end_RNA_polymerase_sigma_factor,_ECF_subfamily_1987121..1987271_POSITIVE 237 178 134 97 54 pint:PI0782|3end_beta-lactamase_induction_signal_transducer,_AmpG_protein_721927..722077_POSITIVE 235 130 83 89 37 pint:PI1198|3end_acetyltransferase_1115219..1115369_POSITIVE 235 55 16 46 5 pint:PI0188|3end_phosphomethylpyrimidine_kinase_179670..179820_POSITIVE