Origin IGS: tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tccacacagcttgcggatgaaatctgcttgctctacggtcagtcgctcaatgacatttcctttatcaagaacttttcttgcaaactcacttatggttactcctgcgtattcagtttcaccgtgatacggtatttcttcttttcggctacacccttggttgggcgaccaccttttttatattctgtttttatcatacttgtttctttaatttgatttgatggagaccagcgggataatcctctgcaccgagtgtcagaaggtggatcaagtggttttggtatgcccaaaacataaacttgctccccgaaaaatagattatgcaaagcaaatcctacaatca Mask Tandem Repeat Region ================================================ tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Find is-nt database================================================ Query_seq: PI0503:PI0504|PI0503:PI0504:mobilizable transposon, excision protein:hypothetical protein:->->:464259..464598 340 tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0503:PI0504|PI0503:PI0504:mobilizable transposon, excision protein:hypothetical protein:->->:464259..464598 340 tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0503:PI0504|PI0503:PI0504:mobilizable transposon, excision protein:hypothetical protein:->->:464259..464598 340 tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 13 End: 147 ............ M L C I I Y F S G S K F M F W A Y Q N H L I H L L T L G A E D Y P A G L H Q I K L K K Q V ................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 3 End: 242 .. M V G F A L H N L F F G E Q V Y V L G I P K P L D P P S D T R C R G L S R W S P S N Q I K E T S M I K T E Y K K G G R P .................................................................................................. Protein_Len: 44 Strand: + Start: 209 End: 340 ................................................................................................................................................................................................................ M P Y H G E T E Y A G V T I S E F A R K V L D K G N V I E R L T V E Q A D F I R K L C G Protein_Len: 37 Strand: - Start: 220 End: 330 ........................................................................................................................................................................................................................... P S V S Y A P T V M L S N A L F T R S L P F T M S R S V T S C A S K M R M .......... Protein_Len: 60 Strand: - Start: 131 End: 319 .................................................................................................................................. V L I I F V S Y L F P P R G V L P T A S F F F Y R I V T F S F V C S Y G Y T L K C S F N K I F S I D N L S Q G Y L L C M ..................... Protein_Len: 46 Strand: - Start: 126 End: 263 ............................................................................................................................. I L N F F C T H Y F C F I F F T T A W G L T Y G F L L F V T D R H F Q I R L L L W L H T Q M ............................................................................. Protein_Len: 42 Strand: - Start: 1 End: 126 S Q L I Q K A Y D I K R P A L K H K P C V L V V Q D V K Q C E T C L I I G S T E M M ...................................................................................................................................................................................................................... tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 80 HP_score: -2.3 Tail_Score: -5.53436 Start: 171 End: 184 Full_Region: tcggctacacccttg gttgg gcga ccacc ttttttatattctgt ..........................................................................................................................................................................gttgggcgaccacc............................................................................................................................................................ Find igs database================================================ Query_seq: PI0503:PI0504|PI0503:PI0504:mobilizable transposon, excision protein:hypothetical protein:->->:464259..464598 340 tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Intra-Species Hit: Count: 1 Min: 1 Max: 340 Len: 340 Subject: pint_PI0503_PI0504|mobilizable transposon, excision protein:hypothetical protein|POSITIVE:POSITIVE|[464259,464598]|340 HSP 1 e-value: 0.0 bit: 674.0 Len: 340 Query Start:1 Query End:340 Subject Strand: POSITIVE Subject Start: 1 Subject End: 340 tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga tgattgtaggatttgctttgcataatctatttttcggggagcaagtttatgttttgggcataccaaaaccacttgatccaccttctgacactcggtgcagaggattatcccgctggtctccatcaaatcaaattaaagaaacaagtatgataaaaacagaatataaaaaaggtggtcgcccaaccaagggtgtagccgaaaagaagaaataccgtatcacggtgaaactgaatacgcaggagtaaccataagtgagtttgcaagaaaagttcttgataaaggaaatgtcattgagcgactgaccgtagagcaagcagatttcatccgcaagctgtgtgga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUGUAGGAUUUGCUUUGCAUAAUCUAUUUUUCGGGGAGCAAGUUUAUGUUUUGGGCAUACCAAAACCACUUGAUCCACCUUCUGACACUCGGUGCAGAGGAUUAUCCCGCUGGUCUCCAUCAAAUCAAAUUAAAGAAACAAGUAUGAUAAAAACAGAAUAUAAAAAAGGUGGUCGCCCAACCAAGGGUGUAGCCGAAAAGAAGAAAUACCGUAUCACGGUGAAACUGAAUACGCAGGAGUAACCAUAAGUGAGUUUGCAAGAAAAGUUCUUGAUAAAGGAAAUGUCAUUGAGCGACUGACCGUAGAGCAAGCAGAUUUCAUCCGCAAGCUGUGUGGA ........((((((((((.((....(((((....(((((..(((((....(((((((.....))))))).)))))......((((((.(((...)))))))))....))))).((((((((.....((((.(((.........))).)))).....(((...........(((...(((((......)))))..)))............(((((.....)))))...)))........))))..))))...((.((((..(((((....(((((....)))))....)).)))...)))).)).)))))))))))))))))..((((((.....)))))) (-89.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCACACAGCUUGCGGAUGAAAUCUGCUUGCUCUACGGUCAGUCGCUCAAUGACAUUUCCUUUAUCAAGAACUUUUCUUGCAAACUCACUUAUGGUUACUCCUGCGUAUUCAGUUUCACCGUGAUACGGUAUUUCUUCUUUUCGGCUACACCCUUGGUUGGGCGACCACCUUUUUUAUAUUCUGUUUUUAUCAUACUUGUUUCUUUAAUUUGAUUUGAUGGAGACCAGCGGGAUAAUCCUCUGCACCGAGUGUCAGAAGGUGGAUCAAGUGGUUUUGGUAUGCCCAAAACAUAAACUUGCUCCCCGAAAAAUAGAUUAUGCAAAGCAAAUCCUACAAUCA .(((...(((..((((((...))))))..)))....((...((((.....))))....)).....(((((.....)))))............))).......((((((.((......((((.....))))........(((((((...(.(((......))).).(((((((((..((((((.((((((((((..(..(((.....)))..)...))))))))))..(((((.......)))))...)))))).)))))))))..(((((.(((((((.....)))))))....)))))....)))))))...)).)))))).................. (-84.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 387 334 291 53 5 pint:PI0586|5end_hypothetical_protein_533757..533987_POSITIVE 383 339 296 169 131 pint:PI1157|5end_hypothetical_protein_1081704..1081934_POSITIVE 349 327 281 64 20 pint:PI0723|5end_conserved_hypothetical_protein_656043..656273_POSITIVE 340 256 212 203 160 pint:PI2006|5end_potassium_uptake_system_protein_1964480..1964710_POSITIVE 340 219 174 126 71 pint:PI0806|5end_heavy_metal_efflux_pump_743679..743909_POSITIVE 336 117 71 131 81 pint:PI1882|5end_hypothetical_protein_1825787..1826017_POSITIVE 334 339 294 66 12 pint:PI0647|5end_potassium_uptake_protein_587505..587735_POSITIVE 324 98 56 129 73 pint:PI1834|5end_anaerobic_C4-dicarboxylate_membrane_transporter_1774861..1775091_POSITIVE 324 339 295 66 22 pint:PI1617|5end_hypothetical_protein_1542923..1543153_POSITIVE 314 114 74 124 78 pint:PI0679|5end_hypothetical_protein_616804..617034_POSITIVE 311 130 86 212 173 pint:PI1540|5end_hypothetical_protein_1461362..1461592_POSITIVE 311 330 291 72 29 pint:PI0708|5end_conserved_hypothetical_protein_644475..644705_POSITIVE 310 119 72 101 50 pint:PI1928|5end_conserved_hypothetical_protein_1871996..1872226_POSITIVE 306 103 82 171 149 pint:PI0654|5end_K+-dependent_Na+/Ca+_exchanger_related-protein_596283..596513_POSITIVE 298 104 65 75 38 pint:PI0035|5end_NAD-dependent_protein_deacetylase,_SIR2_family_37873..38103_POSITIVE 296 340 295 79 27 pint:PI0107|5end_2-methylthioadenine_synthetase_103280..103510_POSITIVE 293 115 71 123 79 pint:PI0539|5end_hypothetical_protein_493406..493636_POSITIVE 291 118 80 211 167 pint:PI1653|5end_hypothetical_protein_1565158..1565388_POSITIVE 289 335 296 143 88 pint:PI0155|5end_conserved_hypothetical_protein_148290..148520_POSITIVE 288 335 292 163 117 pint:PI0227|5end_ABC_transporter,_ATP-binding/permease_protein_223810..224040_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 320 340 291 102 51 pint:PI2167|3end_hypothetical_protein_2111891..2112041_POSITIVE 320 130 82 134 87 pint:PI0036|3end_glutathione_peroxidase_39289..39439_POSITIVE 310 119 72 71 20 pint:PI1927|3end_hypothetical_protein_1872046..1872196_POSITIVE 306 103 82 44 22 pint:PI0653|3end_hypothetical_protein_596236..596386_POSITIVE 302 318 271 83 39 pint:PI0194|3end_2-amino-3-ketobutyrate_coenzyme_A_ligase_(glycine_C-acetyltransferase)_186957..187107_POSITIVE 297 126 81 128 79 pint:PI0537|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_492412..492562_POSITIVE 289 117 80 151 111 pint:PI1957|3end_calcium-transporting_ATPase_1916574..1916724_POSITIVE 289 335 296 84 29 pint:PI0154|3end_possible_UDP-2,3-diacylglucosamine_hydrolase_148311..148461_POSITIVE 285 239 198 134 93 pint:PI1885|3end_cell_division_protein_1827639..1827789_POSITIVE 285 331 292 58 21 pint:PI0174|3end_succinyl-CoA_synthetase_alpha_subunit_167070..167220_POSITIVE 281 340 293 89 50 pint:PI1284|3end_ATP_synthase,_delta_subunit_1200323..1200473_POSITIVE 280 199 171 134 99 pint:PI0582|3end_hypothetical_protein_525214..525364_POSITIVE 280 198 168 64 32 pint:PI0547|3end_fructose-bisphosphate_aldolase_499466..499616_POSITIVE 277 334 294 140 103 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 277 340 299 101 55 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 277 315 271 83 42 pint:PI0377|3end_DNA_primase/mobilizable_transposon,_excision_protein_347823..347973_POSITIVE 276 97 56 58 16 pint:PI1621|3end_hypothetical_protein_1545740..1545890_POSITIVE 276 208 168 101 53 pint:PI1448|3end_hypothetical_protein_1371832..1371982_POSITIVE 276 216 174 104 52 pint:PI0654|3end_K+-dependent_Na+/Ca+_exchanger_related-protein_597223..597373_POSITIVE 276 97 56 58 16 pint:PI0327|3end_hypothetical_protein_309107..309257_POSITIVE