Origin IGS: taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatacttgttattgatttttacggtgcaaatgaaaaaagtgtttcctctgtgtcctaagacactgaagaaacactataaaaacacaataggtttgtgtgtta Mask Tandem Repeat Region ================================================ taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Find is-nt database================================================ Query_seq: PI0500:PI0501|PI0500:PI0501:mobilizable transposon, tnpC protein:excisionase:->->:461468..461569 102 taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0500:PI0501|PI0500:PI0501:mobilizable transposon, tnpC protein:excisionase:->->:461468..461569 102 taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0500:PI0501|PI0500:PI0501:mobilizable transposon, tnpC protein:excisionase:->->:461468..461569 102 taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 2 End: 97 . V C L G I T N K Y H K K L T K P C L P F V K K M Q V T F I L L M ..... taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -15.6 Tail_Score: -5.37593 Start: 29 End: 64 Full_Region: TATTGTGTTTTTATA GTGTTTCTTCAGTGTC TTAG GACACAGAGGAAACAC TTTTTTCATTTGCAC ............................GTGTTTCTTCAGTGTCTTAGGACACAGAGGAAACAC...................................... Find igs database================================================ Query_seq: PI0500:PI0501|PI0500:PI0501:mobilizable transposon, tnpC protein:excisionase:->->:461468..461569 102 taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Intra-Species Hit: Count: 1 Min: 1 Max: 102 Len: 102 Subject: pint_PI0500_PI0501|mobilizable transposon, tnpC protein:excisionase|POSITIVE:POSITIVE|[461468, 461569]|102 HSP 1 e-value: 2.0E-51 bit: 202.0 Len: 102 Query Start:1 Query End:102 Subject Strand: POSITIVE Subject Start: 1 Subject End: 102 taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt taacacacaaacctattgtgtttttatagtgtttcttcagtgtcttaggacacagaggaaacacttttttcatttgcaccgtaaaaatcaataacaagtatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACACAAACCUAUUGUGUUUUUAUAGUGUUUCUUCAGUGUCUUAGGACACAGAGGAAACACUUUUUUCAUUUGCACCGUAAAAAUCAAUAACAAGUAUU .............(((((.(((((((((((((((((((.(((((....))))).)))))))))))...............)))))))))))))......... (-26.76) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUACUUGUUAUUGAUUUUUACGGUGCAAAUGAAAAAAGUGUUUCCUCUGUGUCCUAAGACACUGAAGAAACACUAUAAAAACACAAUAGGUUUGUGUGUUA .....((((.((((.......))))))))........((((((((.((.(((((....))))).)).))))))))......((((((.....)))))).... (-25.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 260 50 4 119 71 pint:PI0973|5end_glycine_dehydrogenase_[decarboxylating]_subunit_1_899483..899713_POSITIVE 251 80 34 119 78 pint:PI1097|5end_acetyltransferase,_GNAT_family_1021443..1021673_POSITIVE 250 60 14 180 135 pint:PI1757|5end_hypothetical_protein_1683934..1684164_POSITIVE 238 79 39 111 72 pint:PI0794|5end_long-chain-fatty-acid--CoA_ligase_731103..731333_POSITIVE 237 80 31 52 1 pint:PI1112|5end_glycosyltransferase_1036443..1036673_POSITIVE 226 82 51 150 121 pint:PI2080|5end_hypothetical_protein_2038093..2038323_POSITIVE 225 96 52 194 148 pint:PI0419|5end_conserved_hypothetical_protein_386701..386931_POSITIVE 225 96 52 159 113 pint:PI0418|5end_hypothetical_protein_386666..386896_POSITIVE 220 89 45 86 44 pint:PI0974|5end_hypothetical_protein_899371..899601_POSITIVE 217 60 13 58 14 pint:PI0585|5end_conserved_hypothetical_protein_532660..532890_POSITIVE 216 70 27 168 107 pint:PI0576|5end_helicase,_UvrD/Rep_520226..520456_POSITIVE 215 81 41 55 5 pint:PI0993|5end_probable_TPR-repeat-containing_protein_917175..917405_POSITIVE 214 78 31 90 40 pint:PI0396|5end_possible_tetracycline_resistance_element_mobilization_regulatory_protein,_C-terminal_fragment_361993..362223_POSITIVE 213 56 11 87 44 pint:PI1601|5end_hypothetical_protein_1530282..1530512_POSITIVE 211 70 26 117 51 pint:PI1992|5end_hypothetical_protein_1946427..1946657_POSITIVE 211 78 32 164 122 pint:PI0188|5end_phosphomethylpyrimidine_kinase_178433..178663_POSITIVE 210 49 4 191 134 pint:PI1662|5end_hypothetical_protein_1576916..1577146_POSITIVE 210 49 4 191 134 pint:PI0369|5end_hypothetical_protein_340084..340314_POSITIVE 208 60 15 69 18 pint:PI1542|5end_mobilization_protein_1461728..1461958_POSITIVE 207 55 11 105 66 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 471 65 28 63 26 pint:PI0500|3end_mobilizable_transposon,_tnpC_protein_461407..461557_POSITIVE 260 50 4 147 99 pint:PI0974|3end_hypothetical_protein_899591..899741_POSITIVE 251 80 34 86 45 pint:PI1096|3end_glycosyltransferase_1021490..1021640_POSITIVE 245 79 34 45 4 pint:PI1136|3end_conserved_hypothetical_protein_1062541..1062691_POSITIVE 227 80 36 39 2 pint:PI1014|3end_prismane_protein,_hybrid-cluster_protein_936132..936282_POSITIVE 225 52 16 143 98 pint:PI1177|3end_hypothtical_protein_1099154..1099304_POSITIVE 225 96 52 143 97 pint:PI0417|3end_hypothetical_protein_386730..386880_POSITIVE 225 96 52 120 74 pint:PI0416|3end_hypothetical_protein_386707..386857_POSITIVE 222 64 27 126 89 pint:PI2083|3end_hypothetical_protein_2040661..2040811_POSITIVE 222 81 41 142 104 pint:PI2074|3end_conserved_hypothetical_protein_2034375..2034525_POSITIVE 220 60 12 141 93 pint:PI2145|3end_hypothetical_protein_2092649..2092799_POSITIVE 217 100 52 71 27 pint:PI1426|3end_flavodoxin_1345446..1345596_POSITIVE 216 70 27 90 29 pint:PI0575|3end_hypothetical_protein_520228..520378_POSITIVE 214 49 5 88 54 pint:PI1521|3end_hypothetical_protein_1441461..1441611_POSITIVE 214 49 5 132 98 pint:PI0659|3end_hypothetical_protein_603396..603546_POSITIVE 211 56 16 48 3 pint:PI1565|3end_conserved_hypothetical_protein;_possible_surface_antigen_1487357..1487507_POSITIVE 208 60 15 145 94 pint:PI1541|3end_hypothetical_protein_1461884..1462034_POSITIVE 208 65 21 85 42 pint:PI0147|3end_HIT_family_protein_141308..141458_POSITIVE 206 78 37 65 28 pint:PI1543|3end_hypothetical_protein_1463281..1463431_POSITIVE 206 76 35 135 86 pint:PI1372|3end_thiamine_monophosphate_kinase_1293971..1294121_POSITIVE