Origin IGS: taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaatatgaatactcttactctgtatttcatactttttcaaatttaatatcgcaaagatacagataatttctctaagagaataatataatcagtaagaatcaatactaaaagataataactaaattatttatccaactgataaagtcatccgctattctctgcaaaaaaacaaaaagaaaggaataaaatacaaaaaactaataggaaagcctttctcataactcgatttccttaatttgattaaaactaaatccttgaagatacagatgattattctccatttacacacaataaggaatattaccataactattattcacttgcctttccctaattgatatttcatctttatatacaggtaaacttgcatatttta Mask Tandem Repeat Region ================================================ taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Find is-nt database================================================ Query_seq: PI0450:PI0451|PI0450:PI0451:hypothetical protein:serine proteases, S51 group:->->:424107..424482 376 taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0450:PI0451|PI0450:PI0451:hypothetical protein:serine proteases, S51 group:->->:424107..424482 376 taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0450:PI0451|PI0450:PI0451:hypothetical protein:serine proteases, S51 group:->->:424107..424482 376 taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Predict ORF larger than 30AA ================================================ Protein_Len: 54 Strand: + Start: 61 End: 222 ............................................................ M I V M V I F L I V C K W R I I I C I F K D L V L I K L R K S S Y E K G F P I S F L Y F I P F F L F F C R E .......................................................................................................................................................... Protein_Len: 32 Strand: - Start: 159 End: 254 .............................................................................................................................................................. S F P K G I L K K Y K I G K K K N K Q L S Y R I V K D T P Y I M .......................................................................................................................... Protein_Len: 33 Strand: - Start: 42 End: 140 ......................................... N P F P L H I I T I T I N R I T H L H L I I M Q I K L S K T K I M ............................................................................................................................................................................................................................................ taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0450:PI0451|PI0450:PI0451:hypothetical protein:serine proteases, S51 group:->->:424107..424482 376 taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattagttttttgtattttattcctttctttttgtttttttgcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Intra-Species Hit: Count: 1 Min: 1 Max: 376 Len: 376 Subject: pint_PI0450_PI0451|hypothetical protein:serine proteases, S51 group|POSITIVE:POSITIVE|[424107,424482]|376 HSP 1 e-value: 7.0E-97 bit: 355.0 Len: 179 Query Start:1 Query End:179 Subject Strand: POSITIVE Subject Start: 1 Subject End: 179 taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattag..................................................................................................................................................................................................... taaaatatgcaagtttacctgtatataaagatgaaatatcaattagggaaaggcaagtgaataatagttatggtaatattccttattgtgtgtaaatggagaataatcatctgtatcttcaaggatttagttttaatcaaattaaggaaatcgagttatgagaaaggctttcctattag..................................................................................................................................................................................................... HSP 2 e-value: 1.0E-86 bit: 321.0 Len: 162 Query Start:215 Query End:376 Subject Strand: POSITIVE Subject Start: 215 Subject End: 376 ......................................................................................................................................................................................................................gcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt ......................................................................................................................................................................................................................gcagagaatagcggatgactttatcagttggataaataatttagttattatcttttagtattgattcttactgattatattattctcttagagaaattatctgtatctttgcgatattaaatttgaaaaagtatgaaatacagagtaagagtattcatattt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUAUGCAAGUUUACCUGUAUAUAAAGAUGAAAUAUCAAUUAGGGAAAGGCAAGUGAAUAAUAGUUAUGGUAAUAUUCCUUAUUGUGUGUAAAUGGAGAAUAAUCAUCUGUAUCUUCAAGGAUUUAGUUUUAAUCAAAUUAAGGAAAUCGAGUUAUGAGAAAGGCUUUCCUAUUAGUUUUUUGUAUUUUAUUCCUUUCUUUUUGUUUUUUUGCAGAGAAUAGCGGAUGACUUUAUCAGUUGGAUAAAUAAUUUAGUUAUUAUCUUUUAGUAUUGAUUCUUACUGAUUAUAUUAUUCUCUUAGAGAAAUUAUCUGUAUCUUUGCGAUAUUAAAUUUGAAAAAGUAUGAAAUACAGAGUAAGAGUAUUCAUAUUU ....(((((((.(....).)))))))....(((((...((.....(((((((.(.........((((..(((((..(((((..((((.......))))..)))))))))))))).((((....((((..(((((............)))))..))))..))))..).))))))).......(((((((((((((..((((.(((..((((..(((((((((...((((((((....((((((.(((..((((....((.......)).....))))...))).)))))).......(((((...))))).)))))))).)))))))))....))))..))))))).)))))))))))))...))...))))).... (-77.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAUGAAUACUCUUACUCUGUAUUUCAUACUUUUUCAAAUUUAAUAUCGCAAAGAUACAGAUAAUUUCUCUAAGAGAAUAAUAUAAUCAGUAAGAAUCAAUACUAAAAGAUAAUAACUAAAUUAUUUAUCCAACUGAUAAAGUCAUCCGCUAUUCUCUGCAAAAAAACAAAAAGAAAGGAAUAAAAUACAAAAAACUAAUAGGAAAGCCUUUCUCAUAACUCGAUUUCCUUAAUUUGAUUAAAACUAAAUCCUUGAAGAUACAGAUGAUUAUUCUCCAUUUACACACAAUAAGGAAUAUUACCAUAACUAUUAUUCACUUGCCUUUCCCUAAUUGAUAUUUCAUCUUUAUAUACAGGUAAACUUGCAUAUUUUA (((((((.......((((.((((((........(((((((......((((.....))))..(((...(((((...))))).....((((((.((((((((.........(((.((((.........((((((.....)))))).........)))).)))..............(((((((............................)))))))........))))..))))...)))))).......))).)))))))...((((((....((...(((.........(((((((((.((.......)).))))).)))).........))).))....))))))....)))))))))).....))))))).. (-47.93) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 288 83 44 206 163 pint:PI1246|5end_conserved_hypothetical_protein;_possible_lipoprotein_1169392..1169622_POSITIVE 266 201 163 44 8 pint:PI0706|5end_pyridoxal_phosphate_biosynthetic_protein_(4-hydroxythreonine-4-phosphate_dehydrogenase)_642226..642456_POSITIVE 265 244 205 136 91 pint:PI2089|5end_conserved_hypothetical_protein_2046200..2046430_POSITIVE 252 229 184 183 146 pint:PI2076|5end_hypothetical_protein_2035403..2035633_POSITIVE 247 69 44 145 121 pint:PI2011|5end_hypothetical_protein_1970429..1970659_POSITIVE 237 242 209 77 38 pint:PI1212|5end_shikimate_5-dehydrogenase_1130674..1130904_POSITIVE 237 70 36 85 50 pint:PI0993|5end_probable_TPR-repeat-containing_protein_917175..917405_POSITIVE 235 228 185 141 91 pint:PI0499|5end_integrase,_C-terminal_region_460237..460467_POSITIVE 235 126 81 207 161 pint:PI0485|5end_30S_ribosomal_protein_S4_444450..444680_POSITIVE 233 99 53 74 30 pint:PI1914|5end_RNA_polymerase,_ECF-type_sigma_factor_1858025..1858255_POSITIVE 232 89 45 83 40 pint:PI0277|5end_hypothetical_protein_264980..265210_POSITIVE 230 147 110 98 54 pint:PI1331|5end_conserved_hypothetical_protein_1248442..1248672_POSITIVE 230 125 83 52 12 pint:PI0749|5end_hypothetical_protein_677234..677464_POSITIVE 227 73 31 226 192 pint:PI2096|5end_conserved_hypothetical_protein;_possible_phosphoesterase_2050268..2050498_POSITIVE 227 238 198 223 188 pint:PI0194|5end_2-amino-3-ketobutyrate_coenzyme_A_ligase_(glycine_C-acetyltransferase)_185804..186034_POSITIVE 226 158 111 73 26 pint:PI1538|5end_conserved_hypothetical_protein_1459387..1459617_POSITIVE 224 253 213 194 143 pint:PI1712|5end_hypothetical_protein_1627497..1627727_POSITIVE 223 248 204 160 119 pint:PI1122|5end_pyridoxal-phosphate-dependent_aminotransferase_1046636..1046866_POSITIVE 223 269 225 97 42 pint:PI0109|5end_hypothetical_protein_106717..106947_POSITIVE 221 100 51 149 96 pint:PI1375|5end_protease_IV_family,_clan_S_(peptidase_family_S49)_1295963..1296193_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 252 229 184 55 18 pint:PI2075|3end_conserved_hypothetical_protein_2035355..2035505_POSITIVE 237 242 209 60 21 pint:PI1211|3end_ubiquinone/menaquinone_biosynthesis_methyltransferase_1130737..1130887_POSITIVE 237 70 36 52 17 pint:PI0992|3end_RNA_polymerase_sigma-70_factor_917222..917372_POSITIVE 232 89 45 44 1 pint:PI0276|3end_anti-restriction_protein_265021..265171_POSITIVE 230 179 146 113 67 pint:PI0133|3end_hypothetical_protein_124632..124782_POSITIVE 227 73 31 94 60 pint:PI2095|3end_hypothetical_protein_2050216..2050366_POSITIVE 223 248 204 151 110 pint:PI1121|3end_glycosyltransferase_1046707..1046857_POSITIVE 222 177 143 54 19 pint:PI1870|3end_hypothetical_protein_1815513..1815663_POSITIVE 218 205 166 69 29 pint:PI1778|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1711399..1711549_POSITIVE 218 217 192 64 39 pint:PI0523|3end_hypothetical_protein_479844..479994_POSITIVE 217 227 188 126 85 pint:PI0658|3end_conserved_hypothetical_protein_603043..603193_POSITIVE 217 59 13 130 75 pint:PI0491|3end_hypothetical_protein_451227..451377_POSITIVE 212 228 182 64 22 pint:PI1644|3end_hypothetical_protein_1561030..1561180_POSITIVE 212 206 163 136 90 pint:PI0498|3end_integrase_460339..460489_POSITIVE 210 124 87 132 81 pint:PI1985|3end_hypothetical_protein_1940745..1940895_POSITIVE 210 174 147 58 30 pint:PI1572|3end_hypothetical_protein_1498215..1498365_POSITIVE 209 238 200 34 1 pint:PI0193|3end_conserved_hypothetical_protein_185695..185845_POSITIVE 207 232 196 146 99 pint:PI1527|3end_immunoreactive_46_kDa_antigen_PG99_1449520..1449670_POSITIVE 207 68 28 92 50 pint:PI1422|3end_hypothetical_protein_1341942..1342092_POSITIVE 207 102 66 149 106 pint:PI0245|3end_possible_mobilization_protein_244258..244408_POSITIVE