Origin IGS: taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 caatacaaaacaaaccgcaagcgttaaatatctcttcataaacttgtttttagttcattacgttacatcaattcaaaagcaaagatagaaaaataggttttaacaacaaaatccaacaactcctattagcctccctgccttgttaattatacataaacaaaagccgaatttgttaaaaacaaagcgtgaaacaatggcaagaaggcggattttacgaactacaaaaatattattcgcaaataagcgcaagattggtgtgcaaacgtaaccttttagcagtgaaacttttgcactcttttatgggatatttgtgcaacaaaacacgcataaaatgttcgtaaaataaacaataaaggtaatatagagaacagaaacagcattgcaaaagctattgttttgcaatgcgaaagcggctcttttgcaatgcaaaagcataggtttgacctgacgaaagcgtaggttttgaaaaacggaaacgattttcttgggaaacaacagcatttttattgtttcacgattctatttcaacgattctgctcaagttttcgtattaaacatttatatacaatctttaagatttcaagaaagaaaatacgaagttcacgaaaccaaacaaagccaaatcaagggataaaatacgtgataaccaaaactttagaggtttattcttttgcgtttctgttatttttcttaactttgtggcacgtgtcggaaaacggcacacagaaccttta Mask Tandem Repeat Region ================================================ taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Find is-nt database================================================ Query_seq: PI0432:PI0434|PI0432:PI0434:hypothetical protein:possible beta-lactamase:->->:405098..405831 734 taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0432:PI0434|PI0432:PI0434:hypothetical protein:possible beta-lactamase:->->:405098..405831 734 taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0432:PI0434|PI0432:PI0434:hypothetical protein:possible beta-lactamase:->->:405098..405831 734 taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: + Start: 11 End: 160 .......... M C R F P T R A T K L R K I T E T Q K N K P L K F W L S R I L S L D L A L F G F V N F V F S F L K S .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 228 End: 434 ................................................................................................................................................................................................................................... M K M L L F P K K I V S V F Q N L R F R Q V K P M L L H C K R A A F A L Q N N S F C N A V S V L Y I T F I V Y F T N I L ............................................................................................................................................................................................................................................................................................................ Protein_Len: 50 Strand: + Start: 310 End: 459 ..................................................................................................................................................................................................................................................................................................................... M A K E P L S H C K T I A F A M L F L F S I L P L L F I L R T F Y A C F V A Q I S H K R V Q K F H C ................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: + Start: 350 End: 502 ............................................................................................................................................................................................................................................................................................................................................................. M Q C C F C S L Y Y L Y C L F Y E H F M R V L L H K Y P I K E C K S F T A K R L R L H T N L A L I C E ........................................................................................................................................................................................................................................ Protein_Len: 59 Strand: + Start: 456 End: 632 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L K G Y V C T P I L R L F A N N I F V V R K I R L L A I V S R F V F N K F G F C L C I I N K A G R L I G V V G F C C ...................................................................................................... Protein_Len: 47 Strand: - Start: 470 End: 610 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... T Q V G I K R K N A F L I K T T R L I R R R A M T E R K T K L L N P K Q K H I I L L A P L S M ............................................................................................................................ Protein_Len: 51 Strand: - Start: 397 End: 549 ............................................................................................................................................................................................................................................................................................................................................................................................................ S C K I R T K N C L Y G M F S H L L K V A L L N R K C V L R A S I Q S Y Y K Q L E Y F G G E Q W Q K M ......................................................................................................................................................................................... Protein_Len: 38 Strand: - Start: 279 End: 392 ...................................................................................................................................................................................................................................................................................... A K T L D F R H K Q M A F S G S E C Q L V I A K A I S N R N E I N G K N N M ...................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: - Start: 104 End: 256 ....................................................................................................... G K I Q S Q K T E H V E Y K R E Q F R L S Q I Y I N L V F V Q A S D N F Y F R S V I F I S N N G L F M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 3 End: 272 .. V Y K I G Q N P K T Q N R S S R I K K R S I K F I T Y L H K I R F S S C F R Q F L I T F C Y F H Q Q K G L F D N G N K M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 33 Strand: - Start: 1 End: 99 L P E T H R K G V R A V F N L F I V S V C F F L G R F N Q N D R M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 62 HP_score: -4.1 Tail_Score: -4.16681 Start: 297 End: 347 Full_Region: TTTCGTCAGGTCAAA CCTATGCTTTTGCATTGCAAAAG AGCCG CTTTCGCATTGCAAAACAATAGC TTTTGCAATGCTGTT ........................................................................................................................................................................................................................................................................................................CCTATGCTTTTGCATTGCAAAAGAGCCGCTTTCGCATTGCAAAACAATAGC................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 64 HP_score: -6.3 Tail_Score: -3.59979 Start: 297 End: 347 Full_Region: aacagcattgcaaaa gctattgttttgcaatgcgaaag cggct cttttgcaatgcaaaagcatagg tttgacctgacgaaa ........................................................................................................................................................................................................................................................................................................gctattgttttgcaatgcgaaagcggctcttttgcaatgcaaaagcatagg................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 66 HP_score: -7.6 Tail_Score: -3.09286 Start: 298 End: 346 Full_Region: TTCGTCAGGTCAAAC CTATGCTTTTGCATTGCAAAAG AGCCG CTTTCGCATTGCAAAACAATAG CTTTTGCAATGCTGT .........................................................................................................................................................................................................................................................................................................CTATGCTTTTGCATTGCAAAAGAGCCGCTTTCGCATTGCAAAACAATAG.................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 75 HP_score: -9.8 Tail_Score: -2.75844 Start: 298 End: 346 Full_Region: acagcattgcaaaag ctattgttttgcaatgcgaaag cggct cttttgcaatgcaaaagcatag gtttgacctgacgaa .........................................................................................................................................................................................................................................................................................................ctattgttttgcaatgcgaaagcggctcttttgcaatgcaaaagcatag.................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 74 HP_score: -6.8 Tail_Score: -4.3277 Start: 329 End: 360 Full_Region: CAAAAGAGCCGCTTT CGCATTGCAAAA CAATAGC TTTTGCAATGCTG TTTCTGTTCTCTATA ........................................................................................................................................................................................................................................................................................................................................CGCATTGCAAAACAATAGCTTTTGCAATGCTG...................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 87 HP_score: -10.5 Tail_Score: -3.75093 Start: 330 End: 358 Full_Region: AAAAGAGCCGCTTTC GCATTGCAAAA CAATAGC TTTTGCAATGC TGTTTCTGTTCTCTA .........................................................................................................................................................................................................................................................................................................................................GCATTGCAAAACAATAGCTTTTGCAATGC........................................................................................................................................................................................................................................................................................................................................................................................ Find igs database================================================ Query_seq: PI0432:PI0434|PI0432:PI0434:hypothetical protein:possible beta-lactamase:->->:405098..405831 734 taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Intra-Species Hit: Count: 2 Min: 1 Max: 734 Len: 734 Subject: pint_PI0432_PI0434|hypothetical protein:possible beta-lactamase|POSITIVE:POSITIVE|[405098,405831]|734 HSP 1 e-value: 0.0 bit: 1455.0 Len: 734 Query Start:1 Query End:734 Subject Strand: POSITIVE Subject Start: 1 Subject End: 734 taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg taaaggttctgtgtgccgttttccgacacgtgccacaaagttaagaaaaataacagaaacgcaaaagaataaacctctaaagttttggttatcacgtattttatcccttgatttggctttgtttggtttcgtgaacttcgtattttctttcttgaaatcttaaagattgtatataaatgtttaatacgaaaacttgagcagaatcgttgaaatagaatcgtgaaacaataaaaatgctgttgtttcccaagaaaatcgtttccgtttttcaaaacctacgctttcgtcaggtcaaacctatgcttttgcattgcaaaagagccgctttcgcattgcaaaacaatagcttttgcaatgctgtttctgttctctatattacctttattgtttattttacgaacattttatgcgtgttttgttgcacaaatatcccataaaagagtgcaaaagtttcactgctaaaaggttacgtttgcacaccaatcttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaacaaggcagggaggctaataggagttgttggattttgttgttaaaacctatttttctatctttgcttttgaattgatgtaacgtaatgaactaaaaacaagtttatgaagagatatttaacgcttgcggtttgttttgtattg Subject: pint_PI1020_PI1021|hypothetical protein:conserved hypothetical protein; possible methyltransferase|POSITIVE:POSITIVE|[938840,939124]|285 HSP 1 e-value: 2.0E-18 bit: 95.6 Len: 108 Query Start:486 Query End:593 Subject Strand: POSITIVE Subject Start: 6 Subject End: 113 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttgcgcttatttgcgaataatatttttgtagttcgtaaaatccgccttcttgccattgtttcacgctttgtttttaacaaattcggcttttgtttatgtataattaac............................................................................................................................................. .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttgcgcttatttacaaataatatttctgttgttcgtaaaatcgcccttcctgccattgtttcacgcttattttttaacaaacaaggcttctgtttgtatataattaac............................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGGUUCUGUGUGCCGUUUUCCGACACGUGCCACAAAGUUAAGAAAAAUAACAGAAACGCAAAAGAAUAAACCUCUAAAGUUUUGGUUAUCACGUAUUUUAUCCCUUGAUUUGGCUUUGUUUGGUUUCGUGAACUUCGUAUUUUCUUUCUUGAAAUCUUAAAGAUUGUAUAUAAAUGUUUAAUACGAAAACUUGAGCAGAAUCGUUGAAAUAGAAUCGUGAAACAAUAAAAAUGCUGUUGUUUCCCAAGAAAAUCGUUUCCGUUUUUCAAAACCUACGCUUUCGUCAGGUCAAACCUAUGCUUUUGCAUUGCAAAAGAGCCGCUUUCGCAUUGCAAAACAAUAGCUUUUGCAAUGCUGUUUCUGUUCUCUAUAUUACCUUUAUUGUUUAUUUUACGAACAUUUUAUGCGUGUUUUGUUGCACAAAUAUCCCAUAAAAGAGUGCAAAAGUUUCACUGCUAAAAGGUUACGUUUGCACACCAAUCUUGCGCUUAUUUGCGAAUAAUAUUUUUGUAGUUCGUAAAAUCCGCCUUCUUGCCAUUGUUUCACGCUUUGUUUUUAACAAAUUCGGCUUUUGUUUAUGUAUAAUUAACAAGGCAGGGAGGCUAAUAGGAGUUGUUGGAUUUUGUUGUUAAAACCUAUUUUUCUAUCUUUGCUUUUGAAUUGAUGUAACGUAAUGAACUAAAAACAAGUUUAUGAAGAGAUAUUUAACGCUUGCGGUUUGUUUUGUAUUG ...(((((.((((.(((((........))).)))))).......(((((((....((((((....(((.......)))....((((((...(((((.(((((((..(.((((((.((((.....((((((((((((((..(((...(..((((.........))))..).)))..)).))))...))).))))).)))).))))))..)..))))))))))))(((((((.......)))))))..))))))....)))))).)))))))...(((((((....))).))))..))))).(((....))).((((((((((((((....(((((((((((.......))))))))))).(((.....(((((...((((.((.(((....(((((((((((.........((((......)))).........(((((((.(((((((.((..(.((......)))..)).)))))))..........(((......))).......))))))).)))))))))))..(((((((((((.(((((.......((((....((((((.......)))))).....))))..))))))))))))))))..(((((((.....((.(((((....)))))))....)))))))...........))).)).))))....(((((((.......)))))))..))))).....)))....))))))).)))))))... (-172.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAAUACAAAACAAACCGCAAGCGUUAAAUAUCUCUUCAUAAACUUGUUUUUAGUUCAUUACGUUACAUCAAUUCAAAAGCAAAGAUAGAAAAAUAGGUUUUAACAACAAAAUCCAACAACUCCUAUUAGCCUCCCUGCCUUGUUAAUUAUACAUAAACAAAAGCCGAAUUUGUUAAAAACAAAGCGUGAAACAAUGGCAAGAAGGCGGAUUUUACGAACUACAAAAAUAUUAUUCGCAAAUAAGCGCAAGAUUGGUGUGCAAACGUAACCUUUUAGCAGUGAAACUUUUGCACUCUUUUAUGGGAUAUUUGUGCAACAAAACACGCAUAAAAUGUUCGUAAAAUAAACAAUAAAGGUAAUAUAGAGAACAGAAACAGCAUUGCAAAAGCUAUUGUUUUGCAAUGCGAAAGCGGCUCUUUUGCAAUGCAAAAGCAUAGGUUUGACCUGACGAAAGCGUAGGUUUUGAAAAACGGAAACGAUUUUCUUGGGAAACAACAGCAUUUUUAUUGUUUCACGAUUCUAUUUCAACGAUUCUGCUCAAGUUUUCGUAUUAAACAUUUAUAUACAAUCUUUAAGAUUUCAAGAAAGAAAAUACGAAGUUCACGAAACCAAACAAAGCCAAAUCAAGGGAUAAAAUACGUGAUAACCAAAACUUUAGAGGUUUAUUCUUUUGCGUUUCUGUUAUUUUUCUUAACUUUGUGGCACGUGUCGGAAAACGGCACACAGAACCUUUA ..............(((((((.(((((.((((((((.((....((((((((((((.((........)).)))).)))))))).............((((((......(((((((..............((((.(.(((((((((...........((((((.......))))))....((.....))..))))).)))).).)))))))))))..(((((.(............(((......))).(((((((.(((((.......((((((..((((........))))....(((((((((...(((((((..........)))))))...)))))))))........))))))...................((((((((((((..((((.....))))((....))....))))))))))))....))))).((((((....(((((((((((((..((((((..((....))......(((.(((((((...........))))))).))).....))))))....)))))....)))))))).)))))).........))))))).....((((......))))....).)))))..))))))............)).))))))))........((((((..((((...(((((.....)))))...))))..))))))....))))))))))))...((((((.....))))))............ (-158.33) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 609 347 304 229 189 pint:PI2046|5end_hypothetical_protein_1999030..1999260_POSITIVE 562 347 304 44 4 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 549 336 302 215 179 pint:PI1437|5end_hypothetical_protein_1356088..1356318_POSITIVE 549 336 302 88 52 pint:PI1436|5end_hypothetical_protein_1355961..1356191_POSITIVE 547 358 312 56 10 pint:PI1733|5end_hypothetical_protein_1652160..1652390_POSITIVE 544 340 294 76 30 pint:PI2144|5end_hypothetical_protein_2091954..2092184_POSITIVE 544 340 294 88 42 pint:PI1934|5end_hypothetical_protein_1882363..1882593_POSITIVE 537 332 294 68 25 pint:PI1528|5end_hypothetical_protein_1449685..1449915_POSITIVE 537 332 294 227 184 pint:PI1486|5end_alginate_O-acetylation_protein_1405507..1405737_POSITIVE 537 230 187 173 130 pint:PI1302|5end_hypothetical_protein_1214797..1215027_POSITIVE 537 332 294 46 3 pint:PI1057|5end_hypothetical_protein_977286..977516_POSITIVE 534 347 307 36 1 pint:PI1041|5end_hypothetical_protein_960443..960673_POSITIVE 514 358 311 89 42 pint:PI1666|5end_conserved_hypothetical_protein_1579231..1579461_POSITIVE 513 357 312 91 46 pint:PI2128|5end_hypothetical_protein_2076512..2076742_POSITIVE 510 347 304 221 183 pint:PI0991|5end_conserved_hypothetical_protein_915309..915539_POSITIVE 505 347 304 94 56 pint:PI2022|5end_hypothetical_protein_1978631..1978861_POSITIVE 505 347 304 48 10 pint:PI2023|5end_hypothetical_protein_1978585..1978815_POSITIVE 502 340 294 88 42 pint:PI1076|5end_hypothetical_protein_998893..999123_POSITIVE 500 336 304 37 5 pint:PI2141|5end_hypothetical_protein_2087169..2087399_POSITIVE 498 336 299 88 52 pint:PI0322|5end_hypothetical_protein_303508..303738_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 512 346 302 45 1 pint:PI1051|3end_hypothetical_protein_970228..970378_POSITIVE 489 358 312 148 102 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 489 358 312 61 15 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 484 336 303 143 110 pint:PI1521|3end_hypothetical_protein_1441461..1441611_POSITIVE 484 336 303 52 19 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 473 349 302 151 102 pint:PI0182|3end_hypothetical_protein_174277..174427_POSITIVE 472 340 294 145 98 pint:PI1356|3end_hypothetical_protein_1273340..1273490_POSITIVE 470 340 294 71 25 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 447 339 304 98 62 pint:PI0567|3end_hypothetical_protein_513055..513205_POSITIVE 445 360 312 65 15 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 434 332 294 127 84 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 433 357 311 120 69 pint:PI0560|3end_hypothetical_protein_508910..509060_POSITIVE 433 341 304 149 112 pint:PI0551|3end_hypothetical_protein_502683..502833_POSITIVE 431 358 312 55 9 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 428 339 304 125 89 pint:PI0819|3end_hypothetical_protein_759919..760069_POSITIVE 415 328 281 59 10 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 413 339 304 42 6 pint:PI0574|3end_hypothetical_protein_519444..519594_POSITIVE 404 341 304 149 112 pint:PI2131|3end_hypothetical_protein_2080868..2081018_POSITIVE 390 341 303 149 111 pint:PI2011|3end_hypothetical_protein_1970730..1970880_POSITIVE 385 341 303 149 111 pint:PI0200|3end_hypothetical_protein_191735..191885_POSITIVE