Origin IGS: tagcctgcacgctccaattcctcctgaaacttttctgcaatctcctttaaaacgctcttggcaccatttgtaagataaggtgcagagtgggtcaatgggtctatcagataatactcgttcgactctatcttcttcagtcttctatggttatcttccagcttctcccttgtgtcaatgggcgtaactccatatttctgtgcagcatacagctgttcgccatgcaggtctttgaagtggtaagcactctgcatctttacattgtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taactatgcaaataagaaaaagatacaacacaaagaagaattggcgaaacgtactctcaggaggtgtatttgcgacagtagtggtgtttgctgcatcgtgtgcaagcagcagaatcagcagtgaaggagaaaccttcagagacgacaatgtaaagatgcagagtgcttaccacttcaaagacctgcatggcgaacagctgtatgctgcacagaaatatggagttacgcccattgacacaagggagaagctggaagataaccatagaagactgaagaagatagagtcgaacgagtattatctgatagacccattgacccactctgcaccttatcttacaaatggtgccaagagcgttttaaaggagattgcagaaaagtttcaggaggaattggagcgtgcaggcta Mask Tandem Repeat Region ================================================ tagcctgcacgctccaattcctcctgaaacttttctgcaatctcctttaaaacgctcttggcaccatttgtaagataaggtgcagagtgggtcaatgggtctatcagataatactcgttcgactctatcttcttcagtcttctatggttatcttccagcttctcccttgtgtcaatgggcgtaactccatatttctgtgcagcatacagctgttcgccatgcaggtctttgaagtggtaagcactctgcatctttacattgtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Find is-nt database================================================ Query_seq: PI0421:PI0423|PI0421:PI0423:hypothetical protein:hypothetical protein:->->:387835..388240 406 tagcctgcacgctccaattcctcctgaaacttttctgcaatctcctttaaaacgctcttggcaccatttgtaagataaggtgcagagtgggtcaatgggtctatcagataatactcgttcgactctatcttcttcagtcttctatggttatcttccagcttctcccttgtgtcaatgggcgtaactccatatttctgtgcagcatacagctgttcgccatgcaggtctttgaagtggtaagcactctgcatctttacattgtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0421:PI0423|PI0421:PI0423:hypothetical protein:hypothetical protein:->->:387835..388240 406 tagcctgcacgctccaattcctcctgaaacttttctgcaatctcctttaaaacgctcttggcaccatttgtaagataaggtgcagagtgggtcaatgggtctatcagataatactcgttcgactctatcttcttcagtcttctatggttatcttccagcttctcccttgtgtcaatgggcgtaactccatatttctgtgcagcatacagctgttcgccatgcaggtctttgaagtggtaagcactctgcatctttacattgtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0421:PI0423|PI0421:PI0423:hypothetical protein:hypothetical protein:->->:387835..388240 406 tagcctgcacgctccaattcctcctgaaacttttctgcaatctcctttaaaacgctcttggcaccatttgtaagataaggtgcagagtgggtcaatgggtctatcagataatactcgttcgactctatcttcttcagtcttctatggttatcttccagcttctcccttgtgtcaatgggcgtaactccatatttctgtgcagcatacagctgttcgccatgcaggtctttgaagtggtaagcactctgcatctttacattgtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 2 Min: 3 Max: 260 Len: 258 Subject: UniRef90_Q64XV5 Cluster: Hypothetical protein; n=2; Bacteroides fragilis|Rep: Hypothetical protein - Bacteroides fragilis HSP 1 e-value: 8.0E-9 bit: 61.6 Len: 258 Query Start:3 Query End:260 Subject Strand: null Subject Start: 40 Subject End: 127 .. N V K M Q S A Y H - - F K D L H G E Q L Y A A Q K Y G V T P I D T R E K L E D N H R R L K K I E S N E Y Y L I D P L T H S A P Y L T N G A K S V L K E I A E K F Q E E L E R A G .................................................................................................................................................. .. N I K G V V S Y K R S F G D L N D V Q L K A A H A W G I A P L A S R E E A E E M D G K L V H I V D N D F Y V V D S L T H S I P Y L V P R A S A L L D T I G A N F L D S L T A K G .................................................................................................................................................. Subject: UniRef90_Q8A002 Cluster: Hypothetical protein; n=1; Bacteroides thetaiotaomicron|Rep: Hypothetical protein - Bacteroides thetaiotaomicron HSP 1 e-value: 7.0E-7 bit: 55.1 Len: 231 Query Start:3 Query End:233 Subject Strand: null Subject Start: 50 Subject End: 126 .. F K D L H G E Q L Y A A Q K Y G V T P I D T R E K L E D N H R R L K K I E S N E Y Y L I D P L T H S A P Y L T N G A K S V L K E I A E K F Q E E L E R A G ............................................................................................................................................................................. .. F P D L N D K H L A V A Q A V G I C P P E D R D A A E K M K E Q L I H I T D N Q F Y T V D S L T H S I P Y L V P R A S E L L D T I G S N F L D S L T A K G ............................................................................................................................................................................. tannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 80 End: 181 ............................................................................... M Q S G S M G L S D N T R S T L S S S V F Y G Y L P A S P L C Q W A ................................................................................................................................................................................................................................. Protein_Len: 37 Strand: + Start: 127 End: 237 .............................................................................................................................. M F F S L L W L S S S F S L V S M G V T P Y F C A A Y S C S P C R S L K W ......................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 66 End: 347 ................................................................. M C K I R C R V G Q W V Y Q I I L V R L Y L L Q S S M V I F Q L L P C V N G R N S I F L C S I Q L F A M Q V F E V V S T ........................................................... Protein_Len: 33 Strand: + Start: 302 End: 400 ............................................................................................................................................................................................................................................................................................................. M H T M Q Q T P L L S Q I H L L R V R F A N S S L C C I F F L F A ...... Protein_Len: 52 Strand: + Start: 250 End: 405 ......................................................................................................................................................................................................................................................... M F T L S S L K V S P S L L I L L L A H D A A N T T T V A N T P P E S T F R Q F F F V L Y L F L I C I V . Protein_Len: 33 Strand: - Start: 286 End: 384 ............................................................................................................................................................................................................................................................................................. Q Q N Q Q K C V I C C V G S S D C I C R R L T R K A L E E K H Q M ...................... Protein_Len: 36 Strand: - Start: 137 End: 244 ........................................................................................................................................ D E I T I K W S R G Q T L P R L E M N R H L M C S N A M C T K S T T L M .................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 3 End: 401 .. L D K F H Y A S Q M K V N D D R F T E G E S S I R S S A C S A A F V V V T A F V G G S L V N R W N K K T N Y R K R I Q M ..... Protein_Len: 32 Strand: - Start: 1 End: 96 L R C A G I G G S V K R C D G K F R E Q C W K Y S L T C L P D M ...................................................................................................................................................................................................................................................................................................................... tannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0421:PI0423|PI0421:PI0423:hypothetical protein:hypothetical protein:->->:387835..388240 406 tannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Intra-Species Hit: Count: 1 Min: 262 Max: 406 Len: 145 Subject: pint_PI0421_PI0423|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[387835,388240]|406 HSP 1 e-value: 1.0E-76 bit: 287.0 Len: 145 Query Start:262 Query End:406 Subject Strand: POSITIVE Subject Start: 262 Subject End: 406 .....................................................................................................................................................................................................................................................................tcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta .....................................................................................................................................................................................................................................................................tcgtctctgaaggtttctccttcactgctgattctgctgcttgcacacgatgcagcaaacaccactactgtcgcaaatacacctcctgagagtacgtttcgccaattcttctttgtgttgtatctttttcttatttgcatagtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCGUCUCUGAAGGUUUCUCCUUCACUGCUGAUUCUGCUGCUUGCACACGAUGCAGCAAACACCACUACUGUCGCAAAUACACCUCCUGAGAGUACGUUUCGCCAAUUCUUCUUUGUGUUGUAUCUUUUUCUUAUUUGCAUAGUUA (((.(..((((((.....))))))..).)))...(((((((((....))).)))))).........(((((.(((((((........((((.((((...(((.((......)).))).)))))))).....)))))))))))).. (-32.72) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUAUGCAAAUAAGAAAAAGAUACAACACAAAGAAGAAUUGGCGAAACGUACUCUCAGGAGGUGUAUUUGCGACAGUAGUGGUGUUUGCUGCAUCGUGUGCAAGCAGCAGAAUCAGCAGUGAAGGAGAAACCUUCAGAGACGA .................................................(((.((((..((((((...(((.(..((.(..((((.((((((((...........))))))))))))..).))..).))).))))))))))))). (-31.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 351 70 21 89 37 pint:PI2017|5end_conserved_hypothetical_protein_1974862..1975092_POSITIVE 348 57 16 57 1 pint:PI0527|5end_outer_membrane_efflux_protein_482375..482605_POSITIVE 339 70 23 213 160 pint:PI1675|5end_TonB-dependent_outer_membrane_receptor_1588107..1588337_POSITIVE 336 89 43 128 84 pint:PI1381|5end_conserved_hypothetical_protein_1303060..1303290_POSITIVE 329 51 20 166 140 pint:PI1945|5end_ribonuclease_HII_1901932..1902162_POSITIVE 329 49 1 225 164 pint:PI0209|5end_hypothetical_protein_202723..202953_POSITIVE 327 59 11 60 3 pint:PI2158|5end_glycosyl_transferase,_group_2_family_protein_2104392..2104622_POSITIVE 326 130 81 40 1 pint:PI2155|5end_hypothetical_protein_2100664..2100894_POSITIVE 321 110 64 73 24 pint:PI0572|5end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_516382..516612_POSITIVE 321 70 26 126 78 pint:PI0108|5end_L-lactate_permease_104892..105122_POSITIVE 318 49 1 188 139 pint:PI1811|5end_conserved_hypothetical_protein_1749518..1749748_POSITIVE 318 58 12 65 19 pint:PI0837|5end_conserved_hypothetical_protein_772308..772538_POSITIVE 314 70 24 120 72 pint:PI0539|5end_hypothetical_protein_493406..493636_POSITIVE 311 49 1 195 142 pint:PI1413|5end_hypothetical_protein_1333727..1333957_POSITIVE 310 89 42 192 133 pint:PI0817|5end_glycosyl_transferase,_group_1_family_protein_756861..757091_POSITIVE 308 55 14 172 129 pint:PI1985|5end_hypothetical_protein_1940291..1940521_POSITIVE 308 55 14 65 22 pint:PI1984|5end_MutT/nudix_family_protein_1940184..1940414_POSITIVE 306 70 27 52 1 pint:PI1640|5end_hypothetical_protein_1558062..1558292_POSITIVE 298 56 16 64 21 pint:PI0522|5end_50S_ribosomal_protein_L19_479004..479234_POSITIVE 297 58 12 165 103 pint:PI1747|5end_membrane-bound_lytic_murein_transglycosylase_D_1667483..1667713_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 351 70 21 80 28 pint:PI2016|3end_ribosomal_large_subunit_pseudouridine_synthase_D_1974933..1975083_POSITIVE 329 51 20 144 118 pint:PI1944|3end_hypothetical_protein_1901990..1902140_POSITIVE 328 57 12 81 40 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 321 110 64 60 11 pint:PI0571|3end_conserved_hypothetical_protein;_possible_permease_516449..516599_POSITIVE 318 58 12 50 4 pint:PI0836|3end_conserved_hypothetical_protein_772373..772523_POSITIVE 316 60 12 136 71 pint:PI1575|3end_LemA_protein_1501852..1502002_POSITIVE 311 98 52 126 85 pint:PI0639|3end_small_heat_shock_protein_583165..583315_POSITIVE 296 58 17 75 32 pint:PI0345|3end_conserved_hypothetical_protein_320809..320959_POSITIVE 292 110 61 139 90 pint:PI1574|3end_conserved_hypothetical_protein_1501226..1501376_POSITIVE 290 70 21 118 64 pint:PI1722|3end_possible_peptidyl-prolyl_cis-trans_isomerase_1640995..1641145_POSITIVE 287 85 42 151 92 pint:PI0087|3end_conserved_hypothetical_protein_86733..86883_POSITIVE 286 74 36 142 105 pint:PI0258|3end_conjugative_transposon_protein,_TraG_252285..252435_POSITIVE 285 56 17 149 109 pint:PI1239|3end_possible_peptidase_C1-like_family_(aminopeptidase_C)_1164780..1164930_POSITIVE 284 58 19 59 12 pint:PI2158|3end_glycosyl_transferase,_group_2_family_protein_2105347..2105497_POSITIVE 284 60 20 74 37 pint:PI0028|3end_aminopeptidase_32727..32877_POSITIVE 283 56 11 92 40 pint:PI1652|3end_hypothetical_protein_1565235..1565385_POSITIVE 282 76 35 73 29 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 278 49 2 120 67 pint:PI1711|3end_ABC_transporter,_ATP-binding_protein_1628429..1628579_POSITIVE 277 75 36 67 33 pint:PI2166|3end_hypothetical_protein_2112073..2112223_POSITIVE 277 56 12 56 7 pint:PI0341|3end_conserved_hypothetical_protein_317615..317765_POSITIVE