Origin IGS: taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatataggtgtgtaagtagaactgtttcagactacaataaaagtagtttaacacaattctgtttacattctattcctcacattctattatatgttttcgcatcgaatcattcgtatcgttttcttcggcaaaggtagtttcgttgtactgcgggcaacaaggtcaagcgacaagccgttagggcaacaatctccacacttcattcttcaggtagtattcttgccctaaaccttgttgtcctagccacctaacctttttattgcctcgaaacgaaaacgaccgacccgacggaaagacgcataaaaaaaggtcggatatgcgagaagcaggtaaaaagaaaatggaaactcaactccctctcctctggaatccgcataaaatcaaaaaaaatcagacaagatgaaacagagatacaaaatatcaatttgggtggcacttgcactctcaggctctctcctttggggcggaagcgtctggctttagctggcagggatatgtgtggaaaatttcctcattcggctgctccttaccatcgctttggctatcgcagtgtacattctgacttatgccctcgttatggttgcactttatcccctatttcatgtataaagaatgaacccaattggaacgtaaattgggctttctccactcaccccgaagtaaggacgggacggataactgttcaagataaacagacggagtaaagcggtcggcggatttgccgaccgctcctctctttcgtgagcatcgggcggacaaagaaaggagcaaagaaaccccaatgaaaaacacttcgttacttttgacagtgagttgagacagctcaaaagtaacaaaaagccaaaattagagatataaaatta Mask Tandem Repeat Region ================================================ taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Find is-nt database================================================ Query_seq: PI0401:PI0402|PI0401:PI0402:conserved hypothetical protein:conserved hypothetical protein:->->:367168..368031 864 taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0401:PI0402|PI0401:PI0402:conserved hypothetical protein:conserved hypothetical protein:->->:367168..368031 864 taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0401:PI0402|PI0401:PI0402:conserved hypothetical protein:conserved hypothetical protein:->->:367168..368031 864 taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 10 End: 261 ......... M S N F G F L L L L S C L N S L S K V T K C F S L G F L C S F L C P P D A H E R E E R S A N P P T A L L R L F I L N S Y ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 42 End: 266 ......................................... M S Q L T V K S N E V F F I G V S L L L S L S A R C S R K R G A V G K S A D R F T P S V Y L E Q L S V P S L L R G E W R ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: + Start: 273 End: 416 ................................................................................................................................................................................................................................................................................ M K C N H N E G I S Q N V H C D S Q S D G K E Q P N E E I F H T Y P C Q L K P D A S A P K E R A ................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 368 End: 604 ............................................................................................................................................................................................................................................................................................................................................................................... M S L P A K A R R F R P K G E S L R V Q V P P K L I F C I S V S S C L I F F D F M R I P E E R E L S F H F L F T C F S H .................................................................................................................................................................................................................................................................... Protein_Len: 54 Strand: + Start: 522 End: 683 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M F F L P A S R I S D L F L C V F P S G R S F S F R G N K K V R W L G Q Q G L G Q E Y Y L K N E V W R L L P ..................................................................................................................................................................................... Protein_Len: 38 Strand: + Start: 661 End: 774 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K C G D C C P N G L S L D L V A R S T T K L P L P K K T I R M I R C E N I .......................................................................................... Protein_Len: 41 Strand: - Start: 686 End: 808 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. R S T A Q G Q Q G C Y L S V V K A S S F S V F S E I R F C I I S H S S Y F T F L M ........................................................ Protein_Len: 53 Strand: - Start: 639 End: 797 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. P L F V V Q L I F H P S Q Q G L P K D S S R T A R L V V F S G K G F F V I R I I R H S F M Y Y F T L F L M ................................................................... Protein_Len: 47 Strand: - Start: 464 End: 604 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................... R T Q N K K I K H P N W L L P L Q T E M K K K G A E R M D S R K K H T K G D P R D N E N R P M .................................................................................................................................................................................................................................................................... Protein_Len: 41 Strand: - Start: 305 End: 427 ................................................................................................................................................................................................................................................................................................................ F T C Q S L W L S P L S C G F S S I K W V Y G Q W S F G S A E A G F S L A Q S H M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 41 Strand: - Start: 258 End: 380 ................................................................................................................................................................................................................................................................. V H F L P Y L A V M V L A Y T L I Y V A I A L A I T L L L R I L F N E V C I G A M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 163 End: 564 .................................................................................................................................................................. A L R K R G L P S L R L T C T G G L N I N Q I E T E D Q R I K K S K I R I G S S L S N L K W K R K V Q K E C I R G K K M ............................................................................................................................................................................................................................................................................................................ Protein_Len: 32 Strand: - Start: 50 End: 145 ................................................. S V T L L L S T N K M P T E K S R E K D A R H E R F L P A T P M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 774 End: 803 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTTTACATTCTATTCCTCACATTCTATTAT............................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 66 HP_score: -2.1 Tail_Score: -4.87893 Start: 24 End: 74 Full_Region: TATCTCTAATTTTGG CTTTTTGTTACTTTTGAGCTGT CTCAACTC ACTGTCAAAAGTAACGAAGTG TTTTTCATTGGGGTT .......................CTTTTTGTTACTTTTGAGCTGTCTCAACTCACTGTCAAAAGTAACGAAGTG...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 56 HP_score: -3.3 Tail_Score: -4.05402 Start: 26 End: 72 Full_Region: TCTCTAATTTTGGCT TTTTGTTACTTTTGAGCTGT CTCAACTC ACTGTCAAAAGTAACGAAG TGTTTTTCATTGGGG .........................TTTTGTTACTTTTGAGCTGTCTCAACTCACTGTCAAAAGTAACGAAG........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 50 HP_score: -4.6 Tail_Score: -3.02518 Start: 27 End: 71 Full_Region: CTCTAATTTTGGCTT TTTGTTACTTTTGAGCTGT CTCAACTC ACTGTCAAAAGTAACGAA GTGTTTTTCATTGGG ..........................TTTGTTACTTTTGAGCTGTCTCAACTCACTGTCAAAAGTAACGAA......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 83 HP_score: -9.0 Tail_Score: -4.19177 Start: 121 End: 171 Full_Region: TGTCCGCCCGATGCT CACGAAAGAGAGGAGCGGTCGGC AAATCC GCCGACCGCTTTACTCCGTCTG TTTATCTTGAACAGT ........................................................................................................................CACGAAAGAGAGGAGCGGTCGGCAAATCCGCCGACCGCTTTACTCCGTCTG..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 93 HP_score: -11.9 Tail_Score: -3.73775 Start: 128 End: 165 Full_Region: caagataaacagacg gagtaaagcggtcggcg gatt tgccgaccgctcctctc tttcgtgagcatcgg ...............................................................................................................................gagtaaagcggtcggcggatttgccgaccgctcctctc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -15.3 Tail_Score: -3.79138 Start: 135 End: 158 Full_Region: TCACGAAAGAGAGGA GCGGTCGGC AAATCC GCCGACCGC TTTACTCCGTCTGTT ......................................................................................................................................GCGGTCGGCAAATCCGCCGACCGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -16.0 Tail_Score: -2.82279 Start: 135 End: 158 Full_Region: aacagacggagtaaa gcggtcggcg gatt tgccgaccgc tcctctctttcgtga ......................................................................................................................................gcggtcggcggatttgccgaccgc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 57 HP_score: -4.5 Tail_Score: -3.65699 Start: 225 End: 246 Full_Region: GGTGAGTGGAGAAAG CCCAATT TACGTTCC AATTGGG TTCATTCTTTATACA ................................................................................................................................................................................................................................CCCAATTTACGTTCCAATTGGG.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 50 HP_score: -4.5 Tail_Score: -2.93393 Start: 225 End: 246 Full_Region: tgtataaagaatgaa cccaatt ggaacgta aattggg ctttctccactcacc ................................................................................................................................................................................................................................cccaattggaacgtaaattggg.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 57 HP_score: -4.7 Tail_Score: -3.71552 Start: 797 End: 853 Full_Region: tgatataggtg tgtaagtagaactgtttcagactac aataaaa gtagtttaacacaattctgtttaca ttctattcctcacat ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgtaagtagaactgtttcagactacaataaaagtagtttaacacaattctgtttaca........... Find igs database================================================ Query_seq: PI0401:PI0402|PI0401:PI0402:conserved hypothetical protein:conserved hypothetical protein:->->:367168..368031 864 taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Intra-Species Hit: Count: 3 Min: 1 Max: 864 Len: 864 Subject: pint_PI0401_PI0402|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[367168,368031]|864 HSP 1 e-value: 0.0 bit: 1624.0 Len: 864 Query Start:1 Query End:864 Subject Strand: POSITIVE Subject Start: 1 Subject End: 864 taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgannnnnnnngattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgaccnnnnnnnatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca taattttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgattttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctatatca Subject: pint_PI0271_PI0272|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[262452,262760]|309 HSP 1 e-value: 8.0E-37 bit: 157.0 Len: 143 Query Start:20 Query End:162 Subject Strand: POSITIVE Subject Start: 168 Subject End: 309 ...................ttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgcttta.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ...................ttggctttttgttacttttgagccgtcccaactcactgtcaaaagtaacgaggtacttttc-ttcgggtttctttgctactttctttgtccgctcgaagctcacgaaagaaagtagcggtcggcagatttgccgaccgcttta.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Subject: pint_PI0282_PI0283|hypothetical protein:Zn-dependent peptidase|POSITIVE:POSITIVE|[266887,267693]|807 HSP 1 e-value: 2.0E-25 bit: 119.0 Len: 116 Query Start:482 Query End:597 Subject Strand: POSITIVE Subject Start: 15 Subject End: 131 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgac-cnnnnnnnatgcgtctttccgtcgggtcggtcgttttcgtttc........................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttc........................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 3 Min: 4 Max: 861 Len: 858 Subject: NC_002950_PG1436_PG1439|ATPase, putative:hypothetical protein|POSITIVE:NEGATIVE|[1523815,1524674]|860 HSP 1 e-value: 0.0 bit: 1584.0 Len: 858 Query Start:4 Query End:861 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 860 ...ttttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaatagggg-ataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctga-nnnnnnnngattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgaccnnnnnnnatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctata... ...ttttatatctctaattttggctttttgttacttttgagctgtctcaactcactgtcaaaagtaacgaagtgtttttcattggggtttctttgctcctttctttgtccgcccgatgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcggggtgagtggagaaagcccaatttacgttccaattgggttcattctttatacatgaaataggggcataaagtgcaaccataacgagggcataagtcagaatgtacactgcgatagccaaagcgatggtaaggagcagccgaatgaggaaattttccacacatatccctgccagctaaagccagacgcttccgccccaaaggagagagcctgagagtgcaagtgccacccaaattgatattttgtatctctgtttcatcttgtctgatttttttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgacctttttttatgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaaaggttaggtggctaggacaacaaggtttagggcaagaatactacctgaagaatgaagtgtggagattgttgccctaacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacgatacgaatgattcgatgcgaaaacatataatagaatgtgaggaatagaatgtaaacagaattgtgttaaactacttttattgtagtctgaaacagttctacttacacacctata... Subject: NC_002950_PG0831_PG0832|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[887972,888769]|798 HSP 1 e-value: 2.0E-12 bit: 75.8 Len: 70 Query Start:482 Query End:551 Subject Strand: POSITIVE Subject Start: 231 Subject End: 303 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gattttatgcggattccagaggagagggagttgagtttc--cattttc-tttttacctgcttctcgcatatcc......................................................................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatcc......................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 9.0E-12 bit: 73.8 Len: 93 Query Start:117 Query End:209 Subject Strand: POSITIVE Subject Start: 121 Subject End: 213 ....................................................................................................................tgctcacgaaagagaggagcggtcggcaaatccgccgaccgctttactccgtctgtttatcttgaacagttatccgtcccgtccttacttcgg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ....................................................................................................................tgctcatgaaagaaagtagcggtggacaaatctgccgaccgctttactctgtctgttcttcttgaacggttacttgttccgtccttacttcgg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG0453_PG0456|hypothetical protein:PHP N-terminal domain protein|POSITIVE:POSITIVE|[496731,497854]|1124 HSP 1 e-value: 4.0E-11 bit: 71.9 Len: 64 Query Start:685 Query End:748 Subject Strand: POSITIVE Subject Start: 685 Subject End: 748 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aacggcttgtcgcttgaccttgttgcccgcagtacaacgaaactacctttgccgaagaaaacga.................................................................................................................... ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aacggcttgccgtttgacctcgttgccggcagtactccgaaactgcctttgccgaagaaaacga.................................................................................................................... HSP 2 e-value: 8.0E-6 bit: 54.0 Len: 47 Query Start:563 Query End:609 Subject Strand: POSITIVE Subject Start: 567 Subject End: 613 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atgcgtctttccgtcgggtcggtcgttttcgtttcgaggcaataaaa............................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atgcgtatttccatcgggccggtcgttttcgtttcaagacaataaaa............................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAUAUCUCUAAUUUUGGCUUUUUGUUACUUUUGAGCUGUCUCAACUCACUGUCAAAAGUAACGAAGUGUUUUUCAUUGGGGUUUCUUUGCUCCUUUCUUUGUCCGCCCGAUGCUCACGAAAGAGAGGAGCGGUCGGCAAAUCCGCCGACCGCUUUACUCCGUCUGUUUAUCUUGAACAGUUAUCCGUCCCGUCCUUACUUCGGGGUGAGUGGAGAAAGCCCAAUUUACGUUCCAAUUGGGUUCAUUCUUUAUACAUGAAAUAGGGGAUAAAGUGCAACCAUAACGAGGGCAUAAGUCAGAAUGUACACUGCGAUAGCCAAAGCGAUGGUAAGGAGCAGCCGAAUGAGGAAAUUUUCCACACAUAUCCCUGCCAGCUAAAGCCAGACGCUUCCGCCCCAAAGGAGAGAGCCUGAGAGUGCAAGUGCCACCCAAAUUGAUAUUUUGUAUCUCUGUUUCAUCUUGUCUGAUUUUUUUUGAUUUUAUGCGGAUUCCAGAGGAGAGGGAGUUGAGUUUCCAUUUUCUUUUUACCUGCUUCUCGCAUAUCCGACCUUUUUUUAUGCGUCUUUCCGUCGGGUCGGUCGUUUUCGUUUCGAGGCAAUAAAAAGGUUAGGUGGCUAGGACAACAAGGUUUAGGGCAAGAAUACUACCUGAAGAAUGAAGUGUGGAGAUUGUUGCCCUAACGGCUUGUCGCUUGACCUUGUUGCCCGCAGUACAACGAAACUACCUUUGCCGAAGAAAACGAUACGAAUGAUUCGAUGCGAAAACAUAUAAUAGAAUGUGAGGAAUAGAAUGUAAACAGAAUUGUGUUAAACUACUUUUAUUGUAGUCUGAAACAGUUCUACUUACACACCUAUA ....(((((........((((..(((((.((((((..(((.((((.....(((......)))...(((((((((......(((((....(((((((((.((((((((.((....((((((....(((..(((((((((((......)))))))))))..)))...((((((.....))))))........((((.........))))))))))(((((((((((((((........)))))))))..))))))............))))))))))....(((((..(...(((....(.(((........))))....)))...)..)))))))))))))).....((.(((.....))).))...))))).....((....)).)))))))))..........)))).)))..))))))))))).))))........)))))........((((((((.((...((((..................(((((....((((((((((((..(((........)))..))).))).))))))....)))))........(((((.((.((((((((((((((.((((..(((((((..(((((......((((..((.(((.((.((((((((((((((((((.(((.((((((.........)).))))..))).))))))))..(((.....))).)))))))))).))..)))...))..))))....)))))...).)))))).)))).)))))))))).)))))).)))))))))....)))))))))).(((((..((((((((.(((.(((((.......))))).))).))))))))..)))))........ (-270.09) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAGGUGUGUAAGUAGAACUGUUUCAGACUACAAUAAAAGUAGUUUAACACAAUUCUGUUUACAUUCUAUUCCUCACAUUCUAUUAUAUGUUUUCGCAUCGAAUCAUUCGUAUCGUUUUCUUCGGCAAAGGUAGUUUCGUUGUACUGCGGGCAACAAGGUCAAGCGACAAGCCGUUAGGGCAACAAUCUCCACACUUCAUUCUUCAGGUAGUAUUCUUGCCCUAAACCUUGUUGUCCUAGCCACCUAACCUUUUUAUUGCCUCGAAACGAAAACGACCGACCCGACGGAAAGACGCAUAAAAAAAGGUCGGAUAUGCGAGAAGCAGGUAAAAAGAAAAUGGAAACUCAACUCCCUCUCCUCUGGAAUCCGCAUAAAAUCAAAAAAAAUCAGACAAGAUGAAACAGAGAUACAAAAUAUCAAUUUGGGUGGCACUUGCACUCUCAGGCUCUCUCCUUUGGGGCGGAAGCGUCUGGCUUUAGCUGGCAGGGAUAUGUGUGGAAAAUUUCCUCAUUCGGCUGCUCCUUACCAUCGCUUUGGCUAUCGCAGUGUACAUUCUGACUUAUGCCCUCGUUAUGGUUGCACUUUAUCCCCUAUUUCAUGUAUAAAGAAUGAACCCAAUUGGAACGUAAAUUGGGCUUUCUCCACUCACCCCGAAGUAAGGACGGGACGGAUAACUGUUCAAGAUAAACAGACGGAGUAAAGCGGUCGGCGGAUUUGCCGACCGCUCCUCUCUUUCGUGAGCAUCGGGCGGACAAAGAAAGGAGCAAAGAAACCCCAAUGAAAAACACUUCGUUACUUUUGACAGUGAGUUGAGACAGCUCAAAAGUAACAAAAAGCCAAAAUUAGAGAUAUAAAA .(((((.((((((((((((.(((.(.(((((((.......))))))).).))).)))))))))))))))))..(((............((((((((((..........(((..((((((((((((((((..(((((........)))))((((((((((((...((.....))..(((((((((.(((((((...............)).)).))).)))))))))))))))))))))...................)))))..)))..)))))))).)))(((((.(((.(((.((..........(((.((((((((.((((.(((............(....)............)))))....)))))))).............)).))).............((((.....))))....((((.(((....)).).)))).))))).))).))))))))))))))).(((((((........(((....(((.(((.....))).)))((...(((((((((((((.((...(((((...(((........)))...)).)))...)).)))))....((((.((((((((((((((...(((((.....(((((((........)))))))....((((......((((.........))))........(((((.......)))))..))))...(((((((((((....)))))))))))....))))))))))).)).))).))).)))).))))))))..))...)))...............((((((((......(((((((...)))))))))))))))..)))))))......)))........ (-240.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 561 170 124 137 88 pint:PI0272|5end_conserved_hypothetical_protein_262621..262851_POSITIVE 417 170 129 210 169 pint:PI0652|5end_conserved_hypothetical_protein_592878..593108_POSITIVE 417 710 662 230 175 pint:PI0051|5end_hypothetical_protein_52607..52837_POSITIVE 407 346 305 60 12 pint:PI1801|5end_30S_ribosomal_protein_S9_1733949..1734179_POSITIVE 407 160 111 197 132 pint:PI0831|5end_hypothetical_protein_768129..768359_POSITIVE 403 165 122 102 46 pint:PI0943|5end_conserved_hypothetical_protein_875153..875383_POSITIVE 399 710 662 75 14 pint:PI0812|5end_conserved_hypothetical_protein;_possible_ferredoxin_750185..750415_POSITIVE 389 118 77 197 156 pint:PI0431|5end_Xaa-Pro_aminopeptidase_403064..403294_POSITIVE 387 158 111 227 179 pint:PI1231|5end_hypothetical_protein_1153175..1153405_POSITIVE 374 710 662 65 15 pint:PI1945|5end_ribonuclease_HII_1901932..1902162_POSITIVE 367 599 561 37 3 pint:PI0686|5end_glycosyl_transferase,_group_2_family_protein_623477..623707_POSITIVE 366 414 372 124 86 pint:PI1575|5end_LemA_protein_1501143..1501373_POSITIVE 360 190 147 47 2 pint:PI0335|5end_hypothetical_protein_313708..313938_POSITIVE 360 148 101 46 1 pint:PI0260|5end_conjugative_transposon_protein,_TraJ_253023..253253_POSITIVE 359 154 111 201 157 pint:PI2112|5end_phosphopantetheine_adenylyltransferase_2063194..2063424_POSITIVE 355 688 643 146 99 pint:PI1818|5end_hypothetical_protein_1756891..1757121_POSITIVE 355 429 387 199 154 pint:PI1000|5end_conserved_hypothetical_protein_923332..923562_POSITIVE 350 430 388 193 143 pint:PI0721|5end_hypothetical_protein_654615..654845_POSITIVE 350 430 388 51 1 pint:PI0722|5end_RNA_polymerase_sigma-70_factor_654473..654703_POSITIVE 350 428 382 62 4 pint:PI0536|5end_hypothetical_protein_492163..492393_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 417 170 129 147 106 pint:PI0651|3end_HD_superfamily_hydrolase_592895..593045_POSITIVE 403 165 122 118 62 pint:PI0944|3end_hypothetical_protein_875249..875399_POSITIVE 371 428 385 116 68 pint:PI1720|3end_conserved_hypothetical_protein_1637992..1638142_POSITIVE 366 414 372 127 89 pint:PI1574|3end_conserved_hypothetical_protein_1501226..1501376_POSITIVE 360 190 147 47 2 pint:PI0333|3end_hypothetical_protein_313788..313938_POSITIVE 355 429 387 123 78 pint:PI0998|3end_possible_flavin_reductase_domain_protein_923336..923486_POSITIVE 350 138 91 85 46 pint:PI0684|3end_coproporphyrinogen_III_oxidase_620507..620657_POSITIVE 349 155 131 50 27 pint:PI1924|3end_cytidine_deaminase_1866495..1866645_POSITIVE 348 618 573 80 32 pint:PI1487|3end_hypothetical_protein_1407940..1408090_POSITIVE 346 150 107 106 59 pint:PI1794|3end_conserved_hypothetical_protein_1726696..1726846_POSITIVE 345 430 385 63 2 pint:PI1827|3end_peptidase,_M23/M37_family,_N-terminal_1768602..1768752_POSITIVE 344 130 81 94 48 pint:PI1572|3end_hypothetical_protein_1498215..1498365_POSITIVE 342 150 107 129 72 pint:PI1918|3end_uracil-DNA_glycosylase_1862224..1862374_POSITIVE 342 432 398 67 25 pint:PI1439|3end_hypothetical_protein_1360346..1360496_POSITIVE 338 710 664 97 45 pint:PI0570|3end_exsB_protein;_possible_aluminum_resistance_protein_515395..515545_POSITIVE 336 160 111 86 33 pint:PI0734|3end_ABC_transporter,_ATP-binding_/_permease_668760..668910_POSITIVE 334 107 63 73 24 pint:PI0093|3end_riboflavin_biosynthesis_protein_91537..91687_POSITIVE 333 456 411 144 101 pint:PI2075|3end_conserved_hypothetical_protein_2035355..2035505_POSITIVE 333 140 95 62 10 pint:PI0040|3end_conserved_hypothetical_protein_(possible_integral_membrane_protein)_41872..42022_POSITIVE 332 427 382 65 24 pint:PI0354|3end_hypothetical_protein_326241..326391_POSITIVE