Origin IGS: taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatatacttttctctttcaccgatattatcctactacattactacaagaagggataatacagtgaaagagaaagggttggtattgtagtcagaaagtaggaataggttattactaccgtctaactacacacaactatgtttttgaggccttcaagtataccgaaagccttataagtagttattctccaagtagtctatcaacagtagagatacgcatctgtttta Mask Tandem Repeat Region ================================================ taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Find is-nt database================================================ Query_seq: PI0384:PI0385|PI0384:PI0385:probable radical SAM domain protein:hypothetical protein:->->:353882..354107 226 taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0384:PI0385|PI0384:PI0385:probable radical SAM domain protein:hypothetical protein:->->:353882..354107 226 taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0384:PI0385|PI0384:PI0385:probable radical SAM domain protein:hypothetical protein:->->:353882..354107 226 taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: - Start: 2 End: 124 . F C I R I E V T S L S S P S Y S S I L S E T Y K F A E F V Y N H T T L R Y Y Y G M ...................................................................................................... taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0384:PI0385|PI0384:PI0385:probable radical SAM domain protein:hypothetical protein:->->:353882..354107 226 taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Intra-Species Hit: Count: 1 Min: 1 Max: 226 Len: 226 Subject: pint_PI0384_PI0385|probable radical SAM domain protein:hypothetical protein|POSITIVE:POSITIVE|[353882,354107]|226 HSP 1 e-value: 1.0E-125 bit: 448.0 Len: 226 Query Start:1 Query End:226 Subject Strand: POSITIVE Subject Start: 1 Subject End: 226 taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca taaaacagatgcgtatctctactgttgatagactacttggagaataactacttataaggctttcggtatacttgaaggcctcaaaaacatagttgtgtgtagttagacggtagtaataacctattcctactttctgactacaataccaaccctttctctttcactgtattatcccttcttgtagtaatgtagtaggataatatcggtgaaagagaaaagtatatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACAGAUGCGUAUCUCUACUGUUGAUAGACUACUUGGAGAAUAACUACUUAUAAGGCUUUCGGUAUACUUGAAGGCCUCAAAAACAUAGUUGUGUGUAGUUAGACGGUAGUAAUAACCUAUUCCUACUUUCUGACUACAAUACCAACCCUUUCUCUUUCACUGUAUUAUCCCUUCUUGUAGUAAUGUAGUAGGAUAAUAUCGGUGAAAGAGAAAAGUAUAUCA ...(((((.............)))))((((.(((..((((...(((((((......((((((((((.....))))))))))........))))))).((((((((((.(((((.((((...)))).))))).))))))))))...))))....(((((((((((((((((((((...((...........))..)))))))).))))))))))))).))).)))). (-70.96) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUAUACUUUUCUCUUUCACCGAUAUUAUCCUACUACAUUACUACAAGAAGGGAUAAUACAGUGAAAGAGAAAGGGUUGGUAUUGUAGUCAGAAAGUAGGAAUAGGUUAUUACUACCGUCUAACUACACACAACUAUGUUUUUGAGGCCUUCAAGUAUACCGAAAGCCUUAUAAGUAGUUAUUCUCCAAGUAGUCUAUCAACAGUAGAGAUACGCAUCUGUUUUA .(((...((((((((((((((.(.(((((((((..................)))))))))).))))))))))))))..(((...((((((.(((..((((.((((...)))).))))..))).))))))...((((((.....((((((.(((.........))).))))))...)))))).....))).(((.(((((.....)))))..)))..)))....... (-63.27) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 290 109 62 169 115 pint:PI1487|5end_hypothetical_protein_1407624..1407854_POSITIVE 288 122 88 42 8 pint:PI0048|5end_conserved_hypothetical_protein_47119..47349_POSITIVE 262 66 22 173 120 pint:PI1443|5end_conserved_hypotehtical_protein;_possible_tonB-dependent_outer_membrane_receptor_1364560..1364790_POSITIVE 262 186 146 213 170 pint:PI0328|5end_conserved_hypothetical_protein_309476..309706_POSITIVE 261 219 188 173 142 pint:PI1559|5end_hypothetical_protein_1476506..1476736_POSITIVE 260 160 111 160 100 pint:PI0809|5end_hypothetical_protein_746630..746860_POSITIVE 259 118 72 149 91 pint:PI1005|5end_hypothetical_protein_926201..926431_POSITIVE 259 82 51 185 157 pint:PI0334|5end_hypothetical_protein_313234..313464_POSITIVE 253 50 10 94 55 pint:PI0387|5end_hypothetical_protein_354631..354861_POSITIVE 253 155 120 137 95 pint:PI0279|5end_hypothetical_protein_265600..265830_POSITIVE 250 97 59 51 20 pint:PI0529|5end_hypothetical_protein_483026..483256_POSITIVE 246 110 70 51 2 pint:PI0676|5end_hypothetical_protein_614576..614806_POSITIVE 244 80 33 181 133 pint:PI1824|5end_ThiJ/PfpI_family_protein_1764479..1764709_POSITIVE 244 66 23 216 164 pint:PI1185|5end_hypothetical_protein_1106076..1106306_POSITIVE 243 139 92 223 174 pint:PI1647|5end_hypothetical_protein_1561564..1561794_POSITIVE 243 95 59 176 129 pint:PI1069|5end_hypothetical_protein_988067..988297_POSITIVE 241 198 152 100 52 pint:PI0378|5end_conserved_hypothetical_protein;_possible_helicase_348026..348256_POSITIVE 241 196 153 165 131 pint:PI0099|5end_hypothetical_protein_95530..95760_POSITIVE 240 175 134 228 186 pint:PI0697|5end_5'-nucleotidase_family_protein_633560..633790_POSITIVE 239 215 176 228 190 pint:PI1142|5end_S1_RNA_binding_domain_protein,_S1_ribosomal_protein_1069892..1070122_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 458 220 175 84 40 pint:PI0730|3end_hypothetical_protein_662166..662316_POSITIVE 327 194 151 87 43 pint:PI0502|3end_conserved_hypothetical_protein;_possible_helicase_463089..463239_POSITIVE 276 43 7 82 46 pint:PI1681|3end_Mg2+/Co2+_transporter_1598129..1598279_POSITIVE 262 66 21 115 72 pint:PI1180|3end_ferric_uptake_regulation_protein_1102409..1102559_POSITIVE 259 118 72 85 27 pint:PI1004|3end_conserved_hypothetical_protein_926217..926367_POSITIVE 259 179 142 73 30 pint:PI0082|3end_conserved_hypothetical_protein_(possible_phosphoglycerol_transferase)_82711..82861_POSITIVE 253 155 120 82 40 pint:PI0278|3end_hypothetical_protein_265625..265775_POSITIVE 250 97 59 67 36 pint:PI0528|3end_hypothetical_protein_483122..483272_POSITIVE 247 96 57 47 10 pint:PI0384|3end_probable_radical_SAM_domain_protein_353821..353971_POSITIVE 243 189 141 142 91 pint:PI1527|3end_immunoreactive_46_kDa_antigen_PG99_1449520..1449670_POSITIVE 242 60 13 58 10 pint:PI0183|3end_hypothetical_protein_174810..174960_POSITIVE 241 196 153 87 53 pint:PI0098|3end_conserved_hypothetical_protein_(possible_glycosyl/glycerophosphate_transferase)_95532..95682_POSITIVE 236 43 6 98 41 pint:PI1106|3end_conserved_hypothetical_protein_1030869..1031019_POSITIVE 236 179 133 131 96 pint:PI0380|3end_conserved_hypothetical_protein_350541..350691_POSITIVE 234 107 64 74 36 pint:PI2083|3end_hypothetical_protein_2040661..2040811_POSITIVE 234 79 31 71 6 pint:PI0834|3end_conserved_hypothetical_protein_771473..771623_POSITIVE 233 209 163 78 22 pint:PI0271|3end_hypothetical_protein_262391..262541_POSITIVE 231 102 63 58 12 pint:PI1871|3end_glycosyl_transferase,_group_1_family_protein_1817133..1817283_POSITIVE 231 111 73 47 1 pint:PI1273|3end_anthranilate_synthase_component_II_1192955..1193105_POSITIVE 228 178 134 130 82 pint:PI1651|3end_hypothetical_protein_1564512..1564662_POSITIVE