Origin IGS: gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taactattatggcaaaaaatacaaataacagaacactggtacatactccttatggcacactgaagcttgcaaaaaacgacgcaaagcaattcaggaaaacagtc Mask Tandem Repeat Region ================================================ gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Find is-nt database================================================ Query_seq: PI0345:PI0346|PI0345:PI0346:conserved hypothetical protein:hypothetical protein:->->:320870..320973 104 gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0345:PI0346|PI0345:PI0346:conserved hypothetical protein:hypothetical protein:->->:320870..320973 104 gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0345:PI0346|PI0345:PI0346:conserved hypothetical protein:hypothetical protein:->->:320870..320973 104 gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 1 End: 99 V T K R F Q K A D N K A L K L T G Y P T H V L T R N N T N K A M M ..... gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0345:PI0346|PI0345:PI0346:conserved hypothetical protein:hypothetical protein:->->:320870..320973 104 gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Intra-Species Hit: Count: 1 Min: 1 Max: 104 Len: 104 Subject: pint_PI0345_PI0346|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[320870,320973]|104 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 104 Query Start:1 Query End:104 Subject Strand: POSITIVE Subject Start: 1 Subject End: 104 gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta gactgttttcctgaattgctttgcgtcgttttttgcaagcttcagtgtgccataaggagtatgtaccagtgttctgttatttgtattttttgccataatagtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACUGUUUUCCUGAAUUGCUUUGCGUCGUUUUUUGCAAGCUUCAGUGUGCCAUAAGGAGUAUGUACCAGUGUUCUGUUAUUUGUAUUUUUUGCCAUAAUAGUUA (((((((...(((((..((((.(((........)))))))))))).......(((((((((((((.(((....))).)))..))))))))))....))))))). (-21.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUAUUAUGGCAAAAAAUACAAAUAACAGAACACUGGUACAUACUCCUUAUGGCACACUGAAGCUUGCAAAAAACGACGCAAAGCAAUUCAGGAAAACAGUC ..................................((((...((((.....)))).....(((((((((((..........)).))))..))))).....)))). (-13.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 313 48 3 86 30 pint:PI0410|5end_glutamyl-tRNA_synthetase_(glutamate--tRNA_ligase)_375947..376177_POSITIVE 307 70 25 177 120 pint:PI0701|5end_hypothetical_protein_638356..638586_POSITIVE 305 78 31 230 183 pint:PI0206|5end_TPR-repeat-containing_protein_198112..198342_POSITIVE 304 47 3 103 62 pint:PI1653|5end_hypothetical_protein_1565158..1565388_POSITIVE 301 58 19 207 157 pint:PI1772|5end_pullulanase_1702257..1702487_POSITIVE 296 59 16 220 179 pint:PI0423|5end_hypothetical_protein_388101..388331_POSITIVE 295 50 3 110 42 pint:PI1363|5end_hypothetical_protein_1281666..1281896_POSITIVE 294 53 11 178 134 pint:PI2133|5end_collagenase_2081106..2081336_POSITIVE 291 50 3 157 100 pint:PI0968|5end_hypothetical_protein_895946..896176_POSITIVE 291 67 21 198 143 pint:PI0036|5end_glutathione_peroxidase_38598..38828_POSITIVE 289 68 27 40 1 pint:PI0588|5end_hypothetical_protein_535016..535246_POSITIVE 288 70 24 215 177 pint:PI2155|5end_hypothetical_protein_2100664..2100894_POSITIVE 288 73 32 135 90 pint:PI1036|5end_ATP-dependent__Lon_protease_954446..954676_POSITIVE 286 76 33 164 119 pint:PI0461|5end_50S_ribosomal_protein_L4_433055..433285_POSITIVE 284 65 22 60 19 pint:PI0009|5end_NAD-dependent_epimerase/dehydratase_family_protein_9653..9883_POSITIVE 281 54 16 92 46 pint:PI0093|5end_riboflavin_biosynthesis_protein_90489..90719_POSITIVE 279 50 4 186 130 pint:PI1846|5end_hypothetical_protein_1791705..1791935_POSITIVE 276 57 11 231 174 pint:PI2105|5end_hypothetical_protein_2059357..2059587_POSITIVE 276 57 11 95 38 pint:PI2106|5end_hypothetical_protein_2059221..2059451_POSITIVE 276 50 1 129 79 pint:PI2062|5end_type_I_restriction-modification_system_2021658..2021888_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 305 78 31 133 86 pint:PI0205|3end_deoxyuridine_5'-triphosphate_nucleotidohydrolase_198095..198245_POSITIVE 304 47 3 100 59 pint:PI1652|3end_hypothetical_protein_1565235..1565385_POSITIVE 301 58 19 144 94 pint:PI1771|3end_alpha-glucosidase_1702274..1702424_POSITIVE 296 59 16 64 23 pint:PI0422|3end_conserved_hypothetical_protein_388025..388175_POSITIVE 294 53 11 131 87 pint:PI2132|3end_hypothetical_protein_2081139..2081289_POSITIVE 289 55 11 85 31 pint:PI0888|3end_hypothetical_protein_818613..818763_POSITIVE 288 73 32 142 97 pint:PI1035|3end_queuine_tRNA-ribosyltransferase_(tRNA-guanine_transglycosylase)_954533..954683_POSITIVE 287 67 25 148 97 pint:PI0700|3end_UDP-glucose_4-epimerase_638413..638563_POSITIVE 277 42 4 136 97 pint:PI1845|3end_hypothetical_protein_1791752..1791902_POSITIVE 277 51 19 129 100 pint:PI1527|3end_immunoreactive_46_kDa_antigen_PG99_1449520..1449670_POSITIVE 276 46 4 61 3 pint:PI0501|3end_excisionase_461863..462013_POSITIVE 274 60 15 55 8 pint:PI2036|3end_hypothetical_protein_1989516..1989666_POSITIVE 274 56 12 136 96 pint:PI1586|3end_possible_HesA/MoeB/ThiF_family_protein_1515183..1515333_POSITIVE 274 62 24 41 6 pint:PI1381|3end_conserved_hypothetical_protein_1303676..1303826_POSITIVE 270 68 22 129 77 pint:PI1768|3end_conserved_hypothetical_protein_1696639..1696789_POSITIVE 269 51 11 139 98 pint:PI1628|3end_hypothetical_protein_1551782..1551932_POSITIVE 268 58 23 113 77 pint:PI1372|3end_thiamine_monophosphate_kinase_1293971..1294121_POSITIVE 268 60 17 68 21 pint:PI1363|3end_hypothetical_protein_1281922..1282072_POSITIVE 267 60 12 139 85 pint:PI0258|3end_conjugative_transposon_protein,_TraG_252285..252435_POSITIVE 266 58 22 36 2 pint:PI1402|3end_3-deoxy-7-phosphoheptulonate_synthase_1323980..1324130_POSITIVE