Origin IGS: cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgacagtttcatcggttcaaaaccaggcatttttcactccaaaaaatgcccaaatacactccgttttcacctcaaaacagcactcttcgtaactcattggtaattaacaagatacaaaaccaataaactttcgctaaaaaacgatagtactttttgggcaaaacgatagtactttttacctaaaatctatcatttaggtctaagtaattggctatgaacagccttatgagttatgaaatgcagtaggtaatgatttagcgacctatcttcaacagtgatatacaacgctttgcataactcgaataacctgttgcaaagataaaggtatatcgtgagaaaaaagaattttctccaaattattttcatagagccgtccgttggggcggctttttgttttccgtgtattccgttttatgataagatgcagtccccgaagactgcgttttttgttttcgccccgcttctccgtctgcacatcctccacttatgcccgtgtattatgtcgggatcggttgttcgttgtcagcggcaaaggtagttccgggcttctacggacaaaaaaggtcggggcggcaagccgtttagacgacaatctccacacttccatttcattgcaggtagtattctcgccgtaaaaccttgtttgtcctaagccctacctttttacgccactgaaacgaaaacgaccggcccgacggaaagacgcataaaaaaaatgtcggatatgcgagaagcagggaaaaagaaaatggaaactcaactccctcacctctaaaatctgcataaaatcaaaaatcagacaag Mask Tandem Repeat Region ================================================ cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Find is-nt database================================================ Query_seq: PI0282:PI0283|PI0282:PI0283:hypothetical protein:Zn-dependent peptidase:->->:266887..267693 807 cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0282:PI0283|PI0282:PI0283:hypothetical protein:Zn-dependent peptidase:->->:266887..267693 807 cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0282:PI0283|PI0282:PI0283:hypothetical protein:Zn-dependent peptidase:->->:266887..267693 807 cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 6 End: 146 ..... M I F D F M Q I L E V R E L S F H F L F P C F S H I R H F F Y A S F R R A G R F R F S G V K R ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 68 End: 193 ................................................................... M L L A Y P T F F L C V F P S G R S F S F Q W R K K V G L R T N K V L R R E Y Y L Q ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 181 End: 279 .................................................................................................................................................................................... M L P A M K W K C G D C R L N G L P P R P F L S V E A R N Y L C R ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 40 Strand: + Start: 198 End: 317 ..................................................................................................................................................................................................... M E V W R L S S K R L A A P T F F V R R S P E L P L P L T T N N R S R H N T R A .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 41 Strand: + Start: 317 End: 439 ............................................................................................................................................................................................................................................................................................................................ M S G G C A D G E A G R K Q K T Q S S G T A S Y H K T E Y T E N K K P P Q R T A L ................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 321 End: 533 ................................................................................................................................................................................................................................................................................................................................ M E D V Q T E K R G E N K K R S L R G L H L I I K R N T R K T K S R P N G R L Y E N N L E K I L F S H D I P L S L Q Q V .................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 400 End: 612 ............................................................................................................................................................................................................................................................................................................................................................................................................... M H G K Q K A A P T D G S M K I I W R K F F F L T I Y L Y L C N R L F E L C K A L Y I T V E D R S L N H Y L L H F I T H ................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 619 End: 708 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L G K K Y Y R F A Q K V L S F F S E S L L V L Y L V N Y Q ................................................................................................... Protein_Len: 31 Strand: + Start: 638 End: 730 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M V L P K K Y Y R F L A K V Y W F C I L L I T N E L R R V L F ............................................................................. Protein_Len: 30 Strand: - Start: 705 End: 794 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ W H T V F L A T K L H F R L T N P C K K S H F I G P K S G M ............. Protein_Len: 32 Strand: - Start: 391 End: 486 ...................................................................................................................................................................................................................................................................................................................................................................................................... L V S Y V S F L F G G W R V A R H F Y N P S F E K K E R Y V K M ................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 270 End: 476 ............................................................................................................................................................................................................................................................................. G S M I C P C L H L I H L R L L P A F V F F A T K P S Q M K D Y F P I C P F C F A A G V S P E I F I I Q L F N K K R V M ........................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 223 End: 318 .............................................................................................................................................................................................................................. V A Q R G S R K Q G Y F G P V V K A A S L S C G I G V Y Y V P M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 173 End: 427 ............................................................................................................................................................................ T R L L G S S G K G S V V F L R D R C L V R A Y T S S T C V S F R P S F L F R L R R P S C R I M F R F V R F V F L R G M ............................................................................................................................................................................................................................................................................................................................................................................................ cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -5.7 Tail_Score: -5.79595 Start: 363 End: 382 Full_Region: cgttttatgataaga tgcagtc cccgaa gactgcg ttttttgttttcgcc ..........................................................................................................................................................................................................................................................................................................................................................................tgcagtccccgaagactgcg......................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -10.7 Tail_Score: -4.28375 Start: 412 End: 441 Full_Region: tctccaaattatttt catagagccgtcc gttg gggcggctttttg ttttccgtgtattcc ...........................................................................................................................................................................................................................................................................................................................................................................................................................catagagccgtccgttggggcggctttttg.............................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -10.5 Tail_Score: -5.48213 Start: 418 End: 435 Full_Region: aattattttcataga gccgtcc gttg gggcggc tttttgttttccgtg .................................................................................................................................................................................................................................................................................................................................................................................................................................gccgtccgttggggcggc.................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: PI0282:PI0283|PI0282:PI0283:hypothetical protein:Zn-dependent peptidase:->->:266887..267693 807 cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Intra-Species Hit: Count: 2 Min: 1 Max: 807 Len: 807 Subject: pint_PI0282_PI0283|hypothetical protein:Zn-dependent peptidase|POSITIVE:POSITIVE|[266887,267693]|807 HSP 1 e-value: 0.0 bit: 1552.0 Len: 807 Query Start:1 Query End:807 Subject Strand: POSITIVE Subject Start: 1 Subject End: 807 cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacannnnnnnnatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta cttgtctgatttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacattttttttatgcgtctttccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataaaacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggctgttcatagccaattacttagacctaaatgatagattttaggtaaaaagtactatcgttttgcccaaaaagtactatcgttttttagcgaaagtttattggttttgtatcttgttaattaccaatgagttacgaagagtgctgttttgaggtgaaaacggagtgtatttgggcattttttggagtgaaaaatgcctggttttgaaccgatgaaactgtcgtta Subject: pint_PI0401_PI0402|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[367168,368031]|864 HSP 1 e-value: 4.0E-29 bit: 131.0 Len: 122 Query Start:10 Query End:131 Subject Strand: POSITIVE Subject Start: 477 Subject End: 597 .........tttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacannnnnnnnatgcgtctttccgtcgggccggtcgttttcgtttc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .........tttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgac-ctttttttatgcgtctttccgtcgggtcggtcgttttcgtttc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 4 Min: 10 Max: 584 Len: 575 Subject: tfor_TF0934_TF0936|hypothetical protein:subtilisin-like serine protease|POSITIVE:POSITIVE|[975268,976007]|740 HSP 1 e-value: 1.0E-120 bit: 432.0 Len: 481 Query Start:104 Query End:584 Subject Strand: POSITIVE Subject Start: 108 Subject End: 587 .......................................................................................................tttccgtcgggccggtcgttttcgtttcagtggcgtaaaaag-gtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataa-aacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggag--aaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggc............................................................................................................................................................................................................................... .......................................................................................................tttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggac-aaaacaaaaaacacagtctttaaggact-cattttatcataagaatagaatacac-aaaaacaaaaagctgccccaa-tgacggct-taaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggc............................................................................................................................................................................................................................... Subject: NC_002950_PG0831_PG0832|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[887972,888769]|798 HSP 1 e-value: 1.0E-117 bit: 424.0 Len: 421 Query Start:14 Query End:434 Subject Strand: POSITIVE Subject Start: 230 Subject End: 651 .............tgattttatgcagattttagaggtgagggagttgagtttcca--ttttcttttt-ccctgcttctcgcatatccgacannnnnnnnatgcgtcttt-ccgtcgggccggtcgttttcgtttcagtggcgtaaaaaggtagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataa-aacggaatacacggaaaacaaaaagccgccccaacggacgg..................................................................................................................................................................................................................................................................................................................................................................................... .............tgattttatgcggattccagaggtgagggagttgagtttcaaccttttcttttttccctgcttcttgcatatccaacatttttt--atgcgttttttccgtcggggcggtcgttctcgtttcaggggcgaaaaaaggtagggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacac-ggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcat-ttaccataagaatagaatacacagaaaacaaaaagccgccctaacggacgg..................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG1436_PG1439|ATPase, putative:hypothetical protein|POSITIVE:NEGATIVE|[1523815,1524674]|860 HSP 1 e-value: 4.0E-29 bit: 131.0 Len: 122 Query Start:10 Query End:131 Subject Strand: NEGATIVE Subject Start: 265 Subject End: 385 .........tttttgattttatgcagattttagaggtgagggagttgagtttccattttctttttccctgcttctcgcatatccgacannnnnnnnatgcgtctttccgtcgggccggtcgttttcgtttc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .........tttttgattttatgcggattccagaggagagggagttgagtttccattttctttttacctgcttctcgcatatccgac-ctttttttatgcgtctttccgtcgggtcggtcgttttcgtttc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG0453_PG0456|hypothetical protein:PHP N-terminal domain protein|POSITIVE:POSITIVE|[496731,497854]|1124 HSP 1 e-value: 5.0E-7 bit: 58.0 Len: 37 Query Start:178 Query End:214 Subject Strand: POSITIVE Subject Start: 647 Subject End: 683 .................................................................................................................................................................................agaatactacctgcaatgaaatggaagtgtggagatt................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. .................................................................................................................................................................................agaatacgacctgtaatgaaatggaagtgtggagatt................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 2.0E-6 bit: 56.0 Len: 36 Query Start:97 Query End:132 Subject Strand: POSITIVE Subject Start: 567 Subject End: 602 ................................................................................................atgcgtctttccgtcgggccggtcgttttcgtttca................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ................................................................................................atgcgtatttccatcgggccggtcgttttcgtttca................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUGAUUUUAUGCAGAUUUUAGAGGUGAGGGAGUUGAGUUUCCAUUUUCUUUUUCCCUGCUUCUCGCAUAUCCGACAUUUUUUUUAUGCGUCUUUCCGUCGGGCCGGUCGUUUUCGUUUCAGUGGCGUAAAAAGGUAGGGCUUAGGACAAACAAGGUUUUACGGCGAGAAUACUACCUGCAAUGAAAUGGAAGUGUGGAGAUUGUCGUCUAAACGGCUUGCCGCCCCGACCUUUUUUGUCCGUAGAAGCCCGGAACUACCUUUGCCGCUGACAACGAACAACCGAUCCCGACAUAAUACACGGGCAUAAGUGGAGGAUGUGCAGACGGAGAAGCGGGGCGAAAACAAAAAACGCAGUCUUCGGGGACUGCAUCUUAUCAUAAAACGGAAUACACGGAAAACAAAAAGCCGCCCCAACGGACGGCUCUAUGAAAAUAAUUUGGAGAAAAUUCUUUUUUCUCACGAUAUACCUUUAUCUUUGCAACAGGUUAUUCGAGUUAUGCAAAGCGUUGUAUAUCACUGUUGAAGAUAGGUCGCUAAAUCAUUACCUACUGCAUUUCAUAACUCAUAAGGC ....(((....((((((......((((.(((((((((...((((((.....((((.(((((((((((((((((((..(((((......((.((.((...((((((.((((.((..((((((((((((((....((((((((((((.(((((((.((((((...(((((((.(((((.((...........)).)))))..((((....))))......)))))))....)))))).)))))))....)))))).....))))))))))))))).))))))).))))..))))))..)).)).)).....)))))..)))))))).....))))))))))).)))).........(((((((....)))))))......(((((...((.......))..........(((((((.....)).))))).)))))........)))))).))))))))).)))).......((((..((((...((((((..(((.((((((......))).))).)))...)))))).)))))))).................)))))).)))............. (-194.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCUUAUGAGUUAUGAAAUGCAGUAGGUAAUGAUUUAGCGACCUAUCUUCAACAGUGAUAUACAACGCUUUGCAUAACUCGAAUAACCUGUUGCAAAGAUAAAGGUAUAUCGUGAGAAAAAAGAAUUUUCUCCAAAUUAUUUUCAUAGAGCCGUCCGUUGGGGCGGCUUUUUGUUUUCCGUGUAUUCCGUUUUAUGAUAAGAUGCAGUCCCCGAAGACUGCGUUUUUUGUUUUCGCCCCGCUUCUCCGUCUGCACAUCCUCCACUUAUGCCCGUGUAUUAUGUCGGGAUCGGUUGUUCGUUGUCAGCGGCAAAGGUAGUUCCGGGCUUCUACGGACAAAAAAGGUCGGGGCGGCAAGCCGUUUAGACGACAAUCUCCACACUUCCAUUUCAUUGCAGGUAGUAUUCUCGCCGUAAAACCUUGUUUGUCCUAAGCCCUACCUUUUUACGCCACUGAAACGAAAACGACCGGCCCGACGGAAAGACGCAUAAAAAAAAUGUCGGAUAUGCGAGAAGCAGGGAAAAAGAAAAUGGAAACUCAACUCCCUCACCUCUAAAAUCUGCAUAAAAUCAAAAA .................((((((.((((((((((((....................(((((((....(((((((.................)))))))......)))))))..(((((((.....))))))).))))))))..((.((((((((((....)))))))))).))......(.((.(((((((((.....(((((((((((......)))))))))))....(((..(((.(((((((.....(((.((((..((.........((((....(((((((.(((((((((((((.((((.((((((((((.....((((((....(((((((.(((((....(((((..(.(((....))).).......((((((.((......)).)))).))......)))))...))))).))))))).)))))))))))))....))).)))))))))..))))))))))))))).....))))..........))..)))).)))))))))).)))..))).))))))))))).).........)))).......))))))........... (-183.24) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 404 343 310 172 136 pint:PI1162|5end_hemolysin-related_protein,_with_CBS_domain_1086443..1086673_POSITIVE 375 255 215 51 12 pint:PI0540|5end_conserved_hypothetical_protein_492955..493185_POSITIVE 375 110 61 203 142 pint:PI0337|5end_hypothetical_protein_314732..314962_POSITIVE 374 148 101 176 132 pint:PI1476|5end_acyl-CoA_synthase/O-succinylbenzoic_acid-CoA_ligase_1397699..1397929_POSITIVE 370 249 203 79 31 pint:PI0812|5end_conserved_hypothetical_protein;_possible_ferredoxin_750185..750415_POSITIVE 367 128 89 193 155 pint:PI1894|5end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino_pimelate_ligase_1836947..1837177_POSITIVE 366 243 204 226 177 pint:PI1000|5end_conserved_hypothetical_protein_923332..923562_POSITIVE 365 119 74 92 56 pint:PI1637|5end_hypothetical_protein_1554500..1554730_POSITIVE 363 220 171 146 99 pint:PI1818|5end_hypothetical_protein_1756891..1757121_POSITIVE 362 128 87 220 168 pint:PI0288|5end_hypothetical_protein_(possible_ISPg3-related_transposase)_272272..272502_POSITIVE 360 250 209 219 168 pint:PI0171|5end_hypothetical_protein_163850..164080_POSITIVE 358 148 103 217 166 pint:PI1721|5end_peptidyl-prolyl_cis-trans_isomerase,_PPIC-type_1638086..1638316_POSITIVE 357 349 301 87 38 pint:PI0668|5end_peptidyl-prolyl_cis-trans_isomerase_SlyD,_FKBP-type_(peptidylprolyl_isomerase)_610216..610446_POSITIVE 355 240 191 61 2 pint:PI1666|5end_conserved_hypothetical_protein_1579231..1579461_POSITIVE 351 130 87 202 164 pint:PI0596|5end_hypothetical_protein_546400..546630_POSITIVE 351 130 88 64 18 pint:PI0262|5end_conserved_hypothetical_protein_254685..254915_POSITIVE 349 270 221 197 132 pint:PI0092|5end_regulatory_protein_RecX_89080..89310_POSITIVE 349 269 221 79 18 pint:PI0052|5end_hypothetical_protein_52312..52542_POSITIVE 346 252 215 65 28 pint:PI0207|5end_conserved_hypothetical_protein_199797..200027_POSITIVE 345 270 225 228 166 pint:PI2126|5end_conserved_hypothetical_protein_2074907..2075137_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 449 337 292 81 33 pint:PI1966|3end_conserved_hypothetical_protein_1924091..1924241_POSITIVE 412 340 291 76 27 pint:PI1475|3end_possible_short_chain_dehydrogenase_1397905..1398055_POSITIVE 367 128 89 138 100 pint:PI1893|3end_phospho-N-acetylmuramoyl-pentapeptide-transferase__1836972..1837122_POSITIVE 366 243 204 150 101 pint:PI0998|3end_possible_flavin_reductase_domain_protein_923336..923486_POSITIVE 360 349 303 91 41 pint:PI0047|3end_phosphomethylpyrimidine_kinase_46781..46931_POSITIVE 351 159 112 88 26 pint:PI1789|3end_hypothetical_protein_1719037..1719187_POSITIVE 351 130 87 128 90 pint:PI0595|3end_conserved_hypothetical_protein_546406..546556_POSITIVE 351 130 88 64 18 pint:PI0261|3end_conjugative_transposon_protein,_TraK_254765..254915_POSITIVE 349 341 302 66 18 pint:PI0947|3end_conserved_hypothetical_protein_880709..880859_POSITIVE 346 252 215 44 7 pint:PI0206|3end_TPR-repeat-containing_protein_199856..200006_POSITIVE 342 253 216 52 18 pint:PI1363|3end_hypothetical_protein_1281922..1282072_POSITIVE 342 130 89 107 69 pint:PI0410|3end_glutamyl-tRNA_synthetase_(glutamate--tRNA_ligase)_377490..377640_POSITIVE 338 337 291 109 63 pint:PI1736|3end_topoisomerase_IV,_subunit_A_1657090..1657240_POSITIVE 337 343 304 131 81 pint:PI2095|3end_hypothetical_protein_2050216..2050366_POSITIVE 335 339 291 107 63 pint:PI1973|3end_conserved_hypothetical_protein_1931171..1931321_POSITIVE 333 231 197 137 99 pint:PI0203|3end_conserved_hypothetical_protein_196064..196214_POSITIVE 332 270 221 117 64 pint:PI2103|3end_hypothetical_protein_2057934..2058084_POSITIVE 330 228 181 99 48 pint:PI0747|3end_possible_mobilization_protein_676702..676852_POSITIVE 328 107 62 150 100 pint:PI0616|3end_electron_transfer_flavoprotein,_beta-subunit_566440..566590_POSITIVE 327 426 386 133 94 pint:PI2170|3end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_2112788..2112938_POSITIVE