Origin IGS: acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttcattttctgtttaatggttctacatatatgtgaggaaaggcgcatgcacgccttggtttgtttccttgaaaaggcagatatggaaacgagcctgtgagggaataaatccccggacaggcatatggtaaggtattccaataagggaacatagacccgcctttcgggatatgtccctccctgtataagggtggagctatatgacgggaggcatacttgcacacgtccacccatccatatgtccacacttgtacccttgcgcaagggtgtgaggggtgacgggtgcaataacttgtacacattctcattcctatttccatttccgtaattgtctgttcaaatggctgcggacaggttttcagacttgggcaggcaggtatgtccaccaacctgcaaacacctgtttgacggggcaaatatagaagcctttatccccttgtttctgccctttttgctaactgcgtacttgtggcttcgatttgcgtatttgtggctccgtaagaggtaagtgctttaggcacaacgcttagtaactttgcacccaagcggtaagccgaagccttcgaggcataaagcgttcttttttgagggactcgacttccgtccttcacggacggatttatttgcgccggagcaaatagcaagatatgtcctgcgttactcgaaataaattcggtaactcagaactcttgctcttgcagagggctgaaaaaactccgaagtcgttttttcttggaagagagaatgccttctcttgaatgccggtgatgccgtgaaacactaaaatccaaagtaaaatgacaaagagacatgtggggagacccctgaaaaaagagaaagccagccattgttgt Mask Tandem Repeat Region ================================================ acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Find is-nt database================================================ Query_seq: PI0246:PI0247|PI0246:PI0247:conserved hypothetical protein:conjugative transposon protein, TraA:->->:244660..245512 853 acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0246:PI0247|PI0246:PI0247:conserved hypothetical protein:conjugative transposon protein, TraA:->->:244660..245512 853 acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0246:PI0247|PI0246:PI0247:conserved hypothetical protein:conjugative transposon protein, TraA:->->:244660..245512 853 acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Predict ORF larger than 30AA ================================================ Protein_Len: 55 Strand: + Start: 7 End: 171 ...... M A G F L F F Q G S P H M S L C H F T L D F S V S R H H R H S R E G I L S S K K K R L R S F F S P L Q E Q E F .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 11 End: 232 .......... M A F S F F R G L P T C L F V I L L W I L V F H G I T G I Q E K A F S L P R K N D F G V F S A L C K S K S S E L P N L F ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 41 Strand: + Start: 216 End: 338 ....................................................................................................................................................................................................................... M C S G A N K S V R E G R K S S P S K K N A L C L E G F G L P L G C K V T K R C A ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 187 End: 423 .......................................................................................................................................................................................... M S S N A G H I L L F A P A Q I N P S V K D G S R V P Q K R T L Y A S K A S A Y R L G A K L L S V V P K A L T S Y G A T .............................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 31 Strand: + Start: 423 End: 515 ...................................................................................................................................................................................................................................................................................................................................................................................................................................... M K A S I F A P S N R C L Q V G G H T C L P K S E N L S A A I .................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 30 Strand: + Start: 566 End: 655 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M A P V T P H T L A Q G Y K C G H M D G W T C A S M P P V I ...................................................................................................................................................................................................... Protein_Len: 49 Strand: + Start: 683 End: 829 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S R K A G L C S L I G I P Y H M P V R G F I P S Q A R F H I C L F K E T N Q G V H A P F L T Y M ........................ Protein_Len: 60 Strand: + Start: 555 End: 851 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Y K L L H P S P L T P L R K G T S V D I W M G G R V Q V C L P S Y S S T L I Q G G T Y P E R R V Y V P L L E Y L T I C .. Protein_Len: 60 Strand: + Start: 439 End: 849 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................... M P R Q T G V C R L V D I P A C P S L K T C P Q P F E Q T I T E M E I G M R M C T S Y C T R H P S H P C A R V Q V W T Y .... Protein_Len: 31 Strand: - Start: 758 End: 850 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... L S T E M D A K E L F C V L A H M R R E E C I H L V M L C F M ... Protein_Len: 34 Strand: - Start: 727 End: 828 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... W I G T R P N I G E C A R K W I Q R K L S V F W P T C A G K R V Y M ......................... Protein_Len: 47 Strand: - Start: 530 End: 670 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. P F P F L F S F T Y L N N C G D G R V G K R L P V L T S M H I P P R T C T H R G D Y L E V R M ....................................................................................................................................................................................... Protein_Len: 35 Strand: - Start: 450 End: 554 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................. V P T Q L N T S M G A Q G L R F V Q G C G N S C V I V S I S I P I L M ........................................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: - Start: 436 End: 579 ................................................................................................................................................................................................................................................................................................................................................................................................................................................... I Q G T L C T N A P Q H V Y R G A W T Q F G T R L W K F L C N R F H F Y S H S H T C T I A G T M .................................................................................................................................................................................................................................................................................. Protein_Len: 59 Strand: - Start: 350 End: 526 ............................................................................................................................................................................................................................................................................................................................................................. R K R L W L Y A F R L W L Y A T L L F P L F L P I F A E I N A G D F L H K C T P P C V Q R G L D S F R D A A M Q V S M ....................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: - Start: 188 End: 319 ........................................................................................................................................................................................... K S Y R L V Y R A I Q E P A F L D T R S P R F D L G E F F F A K H R S P K P K G S P H M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 36 Strand: - Start: 100 End: 207 ................................................................................................... S F A N E R G L F F S K P T K E A R Q L L L L E S N G F K N R T V C S M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: - Start: 90 End: 233 ......................................................................................... R C E L S P M R E E L F F R S R L K K L G R C S C S N Q T V S N I E L L A P C I K S N A G A C M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 52 HP_score: -5.1 Tail_Score: -3.20955 Start: 167 End: 206 Full_Region: CTCTGCAAGAGCAAG AGTTCTGAGTTACCGAA TTTAT TTCGAGTAACGCAGGACA TATCTTGCTATTTGC ......................................................................................................................................................................AGTTCTGAGTTACCGAATTTATTTCGAGTAACGCAGGACA....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 57 HP_score: -6.4 Tail_Score: -3.25073 Start: 168 End: 205 Full_Region: caaatagcaagatat gtcctgcgttactcgaa ataaa ttcggtaactcagaac tcttgctcttgcaga .......................................................................................................................................................................gtcctgcgttactcgaaataaattcggtaactcagaac........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 60 HP_score: -8.6 Tail_Score: -2.5185 Start: 168 End: 205 Full_Region: TCTGCAAGAGCAAGA GTTCTGAGTTACCGAA TTTAT TTCGAGTAACGCAGGAC ATATCTTGCTATTTG .......................................................................................................................................................................GTTCTGAGTTACCGAATTTATTTCGAGTAACGCAGGAC........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 90 HP_score: -10.3 Tail_Score: -4.41348 Start: 237 End: 255 Full_Region: ttgagggactcgact tccgtcc ttcac ggacgga tttatttgcgccgga ............................................................................................................................................................................................................................................tccgtccttcacggacgga...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 53 HP_score: -3.5 Tail_Score: -3.6579 Start: 678 End: 710 Full_Region: ACCCTTATACAGGGA GGGACATATCCCG AAAGG CGGGTCTATGTTCCC TTATTGGAATACCTT .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGGACATATCCCGAAAGGCGGGTCTATGTTCCC............................................................................................................................................... Find igs database================================================ Query_seq: PI0246:PI0247|PI0246:PI0247:conserved hypothetical protein:conjugative transposon protein, TraA:->->:244660..245512 853 acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Intra-Species Hit: Count: 2 Min: 1 Max: 853 Len: 853 Subject: pint_PI0246_PI0247|conserved hypothetical protein:conjugative transposon protein, TraA|POSITIVE:POSITIVE|[244660,245512]|853 HSP 1 e-value: 0.0 bit: 1691.0 Len: 853 Query Start:1 Query End:853 Subject Strand: POSITIVE Subject Start: 1 Subject End: 853 acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa acaacaatggctggctttctcttttttcaggggtctccccacatgtctctttgtcattttactttggattttagtgtttcacggcatcaccggcattcaagagaaggcattctctcttccaagaaaaaacgacttcggagttttttcagccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaatccgtccgtgaaggacggaagtcgagtccctcaaaaaagaacgctttatgcctcgaaggcttcggcttaccgcttgggtgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgcagttagcaaaaagggcagaaacaaggggataaaggcttctatatttgccccgtcaaacaggtgtttgcaggttggtggacatacctgcctgcccaagtctgaaaacctgtccgcagccatttgaacagacaattacggaaatggaaataggaatgagaatgtgtacaagttattgcacccgtcacccctcacacccttgcgcaagggtacaagtgtggacatatggatgggtggacgtgtgcaagtatgcctcccgtcatatagctccacccttatacagggagggacatatcccgaaaggcgggtctatgttcccttattggaataccttaccatatgcctgtccggggatttattccctcacaggctcgtttccatatctgccttttcaaggaaacaaaccaaggcgtgcatgcgcctttcctcacatatatgtagaaccattaaacagaaaatgaa Subject: pint_PI0397_PI0398|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[362960,363517]|558 HSP 1 e-value: 6.0E-19 bit: 97.6 Len: 89 Query Start:149 Query End:237 Subject Strand: POSITIVE Subject Start: 150 Subject End: 238 ....................................................................................................................................................gccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaat........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ....................................................................................................................................................gccctctgcaagagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaat........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 6.0E-19 bit: 97.6 Len: 81 Query Start:315 Query End:395 Subject Strand: POSITIVE Subject Start: 314 Subject End: 394 ..........................................................................................................................................................................................................................................................................................................................tgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgca.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ..........................................................................................................................................................................................................................................................................................................................tgcaaagttactaagtgttgtgcctaaatcccttacctcatacgaagccacaactatccaaatcgaagccacaagtacgca.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 1 Min: 149 Max: 395 Len: 247 Subject: NC_002950_PG1442_PG1444|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1528121,1528885]|765 HSP 1 e-value: 3.0E-21 bit: 105.0 Len: 81 Query Start:315 Query End:395 Subject Strand: NEGATIVE Subject Start: 372 Subject End: 452 ..........................................................................................................................................................................................................................................................................................................................tgcaaagttactaagcgttgtgcctaaagcacttacctcttacggagccacaaatacgcaaatcgaagccacaagtacgca.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ..........................................................................................................................................................................................................................................................................................................................tgcaaagttactaagcgttgtgcctaaatcccttacctcatacgaagccacaactatccaaatcgaagccacaagtacgca.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-16 bit: 89.7 Len: 89 Query Start:149 Query End:237 Subject Strand: NEGATIVE Subject Start: 528 Subject End: 616 ....................................................................................................................................................gccctctgcaagagcaagagttctgagttaccgaatttatttcgagtaacgcaggacatatcttgctatttgctccggcgcaaataaat........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ....................................................................................................................................................gccctctgcaggagcaagggttttgagttaccgaaataatttcgggtaacgcaagacataccttgctatttgcactgacgcaaataaat........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCCUCUGCAAGAGCAAGAGUUCUGAGUUACCGAAUUUAUUUCGAGUAACGCAGGACAUAUCUUGCUAUUUGCUCCGGCGCAAAUAAAUCCGUCCGUGAAGGACGGAAGUCGAGUCCCUCAAAAAAGAACGCUUUAUGCCUCGAAGGCUUCGGCUUACCGCUUGGGUGCAAAGUUACUAAGCGUUGUGCCUAAAGCACUUACCUCUUACGGAGCCACAAAUACGCAAAUCGAAGCCACAAGUACGCA (((....((((((((((((((((((.(((((((((.....)))).))))).))))))...))))))).)))))...)))((.......(((((((.....)))))))..(((((....((......))..((.....))))))).(((((((..((...(((((((..(((.(((....))).)))..))).))))......(((.....)))............))..)))))))........)). (-86.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCGUACUUGUGGCUUCGAUUUGCGUAUUUGUGGCUCCGUAAGAGGUAAGUGCUUUAGGCACAACGCUUAGUAACUUUGCACCCAAGCGGUAAGCCGAAGCCUUCGAGGCAUAAAGCGUUCUUUUUUGAGGGACUCGACUUCCGUCCUUCACGGACGGAUUUAUUUGCGCCGGAGCAAAUAGCAAGAUAUGUCCUGCGUUACUCGAAAUAAAUUCGGUAACUCAGAACUCUUGCUCUUGCAGAGGGC ((((((.(((......)))..))))))....(((((((((..(.(((((((...((((((.....))))))..))))...))))..))))..)))))..((((((..(((.....(.(((((((...))))))).)....(((((((.....))))))).........)))...((((..(((((((...((.(((.(((((.((((.....))))))))).))).))))))))).)))).)))))) (-83.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 386 189 142 116 60 pint:PI1985|5end_hypothetical_protein_1940291..1940521_POSITIVE 383 168 126 109 69 pint:PI2021|5end_DNA_repair_protein_1977925..1978155_POSITIVE 382 204 163 147 101 pint:PI1642|5end_conserved_hypothetical_protein_1558800..1559030_POSITIVE 371 204 163 147 101 pint:PI0348|5end_conserved_hypothetical_protein_321572..321802_POSITIVE 368 177 137 168 128 pint:PI0435|5end_30S_ribosomal_protein_S21_406997..407227_POSITIVE 366 200 153 54 6 pint:PI0614|5end_glucose-6-phosphate_isomerase_563170..563400_POSITIVE 365 189 142 168 116 pint:PI0539|5end_hypothetical_protein_493406..493636_POSITIVE 355 220 179 63 23 pint:PI1823|5end_inorganic_polyphosphate/ATP-NAD_kinase_(Poly(P)/ATP_NAD_kinase)_1763238..1763468_POSITIVE 349 180 131 94 46 pint:PI2053|5end_conserved_hypothetical_protein;_possible_outer_membrane_receptor_protein_2010673..2010903_POSITIVE 347 169 129 66 30 pint:PI0529|5end_hypothetical_protein_483026..483256_POSITIVE 347 168 131 223 174 pint:PI0250|5end_hypothetical_protein_246946..247176_POSITIVE 346 166 127 151 105 pint:PI1591|5end_ISPg3-related_transposase_1518747..1518977_POSITIVE 345 168 126 211 166 pint:PI1187|5end_conserved_hypothetical_protein_1106692..1106922_POSITIVE 342 189 146 76 20 pint:PI1659|5end_hypothetical_protein_1574725..1574955_POSITIVE 340 190 147 177 135 pint:PI0092|5end_regulatory_protein_RecX_89080..89310_POSITIVE 339 169 128 58 15 pint:PI0485|5end_30S_ribosomal_protein_S4_444450..444680_POSITIVE 333 170 129 184 138 pint:PI2170|5end_RNA_polymerase_sigma-70_factor,_ECF_subfamily_2112238..2112468_POSITIVE 333 197 153 184 146 pint:PI1831|5end_conserved_hypothetical_protein_1770298..1770528_POSITIVE 332 170 130 216 179 pint:PI2123|5end_adenine_phosphoribosyltransferase_2071711..2071941_POSITIVE 332 189 149 166 129 pint:PI1858|5end_conserved_hypothetical_protein_1804400..1804630_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 382 204 163 150 104 pint:PI1641|3end_hypothetical_protein_1558883..1559033_POSITIVE 371 204 163 150 104 pint:PI0347|3end_hypothetical_protein_321655..321805_POSITIVE 347 169 129 82 46 pint:PI0528|3end_hypothetical_protein_483122..483272_POSITIVE 342 166 130 147 107 pint:PI0091|3end_hypothetical_protein_89774..89924_POSITIVE 336 170 127 98 43 pint:PI1734|3end_possible_carboxyl-terminal_protease-related_protein_1653829..1653979_POSITIVE 332 189 149 82 45 pint:PI1857|3end_guanylate_kinase_1804396..1804546_POSITIVE 331 189 145 70 24 pint:PI0273|3end_hypothetical_protein_263284..263434_POSITIVE 318 113 71 106 57 pint:PI1663|3end_hypothetical_protein_1577347..1577497_POSITIVE 315 120 71 148 101 pint:PI2096|3end_conserved_hypothetical_protein;_possible_phosphoesterase_2050884..2051034_POSITIVE 311 106 62 85 32 pint:PI1440|3end_conserved_hypothetical_protein_1363009..1363159_POSITIVE 309 103 62 85 42 pint:PI0028|3end_aminopeptidase_32727..32877_POSITIVE 307 214 181 68 37 pint:PI0316|3end_conserved_hypothetical_protein_299082..299232_POSITIVE 305 189 146 147 100 pint:PI1684|3end_dimethyladenosine_transferase_1601773..1601923_POSITIVE 304 148 101 134 83 pint:PI0242|3end_hypothetical_protein_240227..240377_POSITIVE 301 82 44 98 61 pint:PI1408|3end_glucose-1-phosphate_thymidylyltransferase_1329761..1329911_POSITIVE 301 169 130 85 39 pint:PI0728|3end_integrase_661508..661658_POSITIVE 300 170 125 147 94 pint:PI0121|3end_outer_membrane_protein,_ompH_family_113208..113358_POSITIVE 295 168 140 25 1 pint:PI0843|3end_carboxyl-terminal_protease_777862..778012_POSITIVE 295 190 141 126 73 pint:PI0687|3end_hypothetical_protein_625652..625802_POSITIVE 294 177 135 54 3 pint:PI0520|3end_hypothetical_protein_478951..479101_POSITIVE