Origin IGS: taaaatgtagctataatgatatataaaaagttgcacatatcgttgatttttagtaacttattgaagtcaaaaccattaagtacaaatcctcgtatatgcaacttatacacaaaattcgtcaaaatccttgagatatgcaagcaattctctcacaaccttgtcaataagcagggaaatataccccgtcgtggtcccatgccaaaattttcagatttggaggtcgttgccttgtccttgacagcggaatcagagagcattgatagtgaaaaatggctttttgattataagcttcaggagtacaaagacaaaatacctaacctcatatctcgcaggcaattcaacgaccgtagaaagaataccgcaggattatgcgaagacatacgaaagagaatcgctgcccaaatggacggtggtgaaactcaattcttcgtagattccaagccgatagccgtatgcagggttgccagagggaagcattgcaagatgggcagacgggagactgttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgactcgtttattttcaggtgttttcttattttgcaaagatactaaaaagtcttgaatatcaaagtgtacttatctaacaaaaaacaggcaaaaatgggctaatttggtaaaaatgagaaaaactcaaattactgattatcagcattatatattcttttcgtttctcttgttttcactatcttgtaccgggtctgggtaccaagtaaaaagctaagtagtttgtttccaaacacttagcttttttcttatttcataaatgttcccaaattgcctgggaacagtctcccgtctgcccatcttgcaatgcttccctctggcaaccctgcatacggctatcggcttggaatctacgaagaattgagtttcaccaccgtccatttgggcagcgattctctttcgtatgtcttcgcataatcctgcggtattctttctacggtcgttgaattgcctgcgagatatgaggttaggtattttgtctttgtactcctgaagcttataatcaaaaagccatttttcactatcaatgctctctgattccgctgtcaaggacaaggcaacgacctccaaatctgaaaattttggcatgggaccacgacggggtatatttccctgcttattgacaaggttgtgagagaattgcttgcatatctcaaggattttgacgaattttgtgtataagttgcatatacgaggatttgtacttaatggttttgacttcaataagttactaaaaatcaacgatatgtgcaactttttatatatcattatagctacatttta Mask Tandem Repeat Region ================================================ taaaatgtagctataatgatatataaaaagttgcacatatcgttgatttttagtaacttattgaagtcaaaaccattaagtacaaatcctcgtatatgcaacttatacacaaaattcgtcaaaatccttgagatatgcaagcaattctctcacaaccttgtcaataagcagggaaatataccccgtcgtggtcccatgccaaaattttcagatttggaggtcgttgccttgtccttgacagcggaatcagagagcattgatagtgaaaaatggctttttgattataagcttcaggagtacaaagacaaaatacctaacctcatatctcgcaggcaattcaacgaccgtagaaagaataccgcaggattatgcgaagacatacgaaagagaatcgctgcccaaatggacggtggtgaaactcaattcttcgtagattccaagccgatagccgtatgcagggttgccagagggaagcattgcaagatgggcagacgggagactgttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Find is-nt database================================================ Query_seq: PI0233:PI0234|PI0233:PI0234:membrane protein, possible exporter:integrase:->->:233832..234612 781 taaaatgtagctataatgatatataaaaagttgcacatatcgttgatttttagtaacttattgaagtcaaaaccattaagtacaaatcctcgtatatgcaacttatacacaaaattcgtcaaaatccttgagatatgcaagcaattctctcacaaccttgtcaataagcagggaaatataccccgtcgtggtcccatgccaaaattttcagatttggaggtcgttgccttgtccttgacagcggaatcagagagcattgatagtgaaaaatggctttttgattataagcttcaggagtacaaagacaaaatacctaacctcatatctcgcaggcaattcaacgaccgtagaaagaataccgcaggattatgcgaagacatacgaaagagaatcgctgcccaaatggacggtggtgaaactcaattcttcgtagattccaagccgatagccgtatgcagggttgccagagggaagcattgcaagatgggcagacgggagactgttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 24 Max: 150 Len: 127 Subject: IS|IS1187 HSP 1 e-value: 2.0E-18 bit: 93.7 Len: 127 Query Start:24 Query End:150 Subject Strand: POSITIVE Subject Start: 39 Subject End: 165 .......................taaaaagttgcacatatcgttgatttttagtaacttattgaagtcaaaaccattaagtacaaatcctcgtatatgcaacttatacacaaaattcgtcaaaatccttgagatatgcaagcaattctct....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .......................taaaaagttgtacatgtcgctgatttttagtaacttagtggtgattaaaacattaagttcaaaccatcgtatgtacaacctttatgcaaaattcgtcaaaatacttgagatatgcaagcaattctct....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find is-aa database================================================ Query_seq: PI0233:PI0234|PI0233:PI0234:membrane protein, possible exporter:integrase:->->:233832..234612 781 taaaatgtagctataatgatatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncacaaccttgtcaataagcagggaaatataccccgtcgtggtcccatgccaaaattttcagatttggaggtcgttgccttgtccttgacagcggaatcagagagcattgatagtgaaaaatggctttttgattataagcttcaggagtacaaagacaaaatacctaacctcatatctcgcaggcaattcaacgaccgtagaaagaataccgcaggattatgcgaagacatacgaaagagaatcgctgcccaaatggacggtggtgaaactcaattcttcgtagattccaagccgatagccgtatgcagggttgccagagggaagcattgcaagatgggcagacgggagactgttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 14 Min: 154 Max: 501 Len: 348 Subject: UniRef90_Q9X6Y4 Cluster: Transposase; n=1; Bacteroides fragilis|Rep: Transposase - Bacteroides fragilis HSP 1 e-value: 1.0E-53 bit: 206.0 Len: 339 Query Start:154 Query End:492 Subject Strand: null Subject Start: 46 Subject End: 158 ......................................................................................................................................................... N L V N K Q G N I P R R G P M P K F S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F F V D S K P I A V C R V A R G K H C K M G R ................................................................................................................................................................................................................................................................................................. ......................................................................................................................................................... N L V N D S G N V P R R G P V P K F S D L E V V A L S L T A E T E S I D S E K W L F D Y K L Q E Y K D S I P N L I S R R Q F N D R R K K T S G L C E E L R K R I A M E M D G G E E Q F F V D S K P I E V C R V A R G K R C K M G R ................................................................................................................................................................................................................................................................................................. Subject: IS|IS1187.aa1 HSP 1 e-value: 1.0E-53 bit: 206.0 Len: 339 Query Start:154 Query End:492 Subject Strand: null Subject Start: 46 Subject End: 158 ......................................................................................................................................................... N L V N K Q G N I P R R G P M P K F S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F F V D S K P I A V C R V A R G K H C K M G R ................................................................................................................................................................................................................................................................................................. ......................................................................................................................................................... N L V N D S G N V P R R G P V P K F S D L E V V A L S L T A E T E S I D S E K W L F D Y K L Q E Y K D S I P N L I S R R Q F N D R R K K T S G L C E E L R K R I A M E M D G G E E Q F F V D S K P I E V C R V A R G K R C K M G R ................................................................................................................................................................................................................................................................................................. Subject: gi|10956806|ref|NP_052841.1| transposase [Riemerella anatipestifer] HSP 1 e-value: 9.0E-12 bit: 67.4 Len: 303 Query Start:199 Query End:501 Subject Strand: null Subject Start: 31 Subject End: 127 ...................................................................................................................................................................................................... P K F S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F F V D S K P I A V C R V A R G K H C K M G R R E T ........................................................................................................................................................................................................................................................................................ ...................................................................................................................................................................................................... P K M S D L E V I S L N I T A E Y L S I D S E L Q L F - - - - R K L P N S L I N K I E R S V Y N K R K R R L S L Q T E Q I R Q R I S M E F N E F E D I F I V D S M P M K V C E N A R S T R S K I C K E Q S ........................................................................................................................................................................................................................................................................................ Subject: UniRef90_Q93T69 Cluster: Transposase; n=2; Riemerella anatipestifer|Rep: Transposase - Riemerella anatipestifer HSP 1 e-value: 9.0E-12 bit: 67.4 Len: 303 Query Start:199 Query End:501 Subject Strand: null Subject Start: 31 Subject End: 127 ...................................................................................................................................................................................................... P K F S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F F V D S K P I A V C R V A R G K H C K M G R R E T ........................................................................................................................................................................................................................................................................................ ...................................................................................................................................................................................................... P K M S D L E V I S L N I T A E Y L S I D S E L Q L F - - - - R K L P N S L I N K I E R S V Y N K R K R R L S L Q T E Q I R Q R I S M E F N E F E D I F I V D S M P M K V C E N A R S T R S K I C K E Q S ........................................................................................................................................................................................................................................................................................ Subject: IS|ISra1.aa1 HSP 1 e-value: 9.0E-12 bit: 67.4 Len: 303 Query Start:199 Query End:501 Subject Strand: null Subject Start: 31 Subject End: 127 ...................................................................................................................................................................................................... P K F S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F F V D S K P I A V C R V A R G K H C K M G R R E T ........................................................................................................................................................................................................................................................................................ ...................................................................................................................................................................................................... P K M S D L E V I S L N I T A E Y L S I D S E L Q L F - - - - R K L P N S L I N K I E R S V Y N K R K R R L S L Q T E Q I R Q R I S M E F N E F E D I F I V D S M P M K V C E N A R S T R S K I C K E Q S ........................................................................................................................................................................................................................................................................................ Subject: gi|67873395|ref|ZP_00503674.1| Transposase, IS4 [Clostridium thermocellum ATCC 27405] HSP 1 e-value: 1.0E-7 bit: 53.9 Len: 285 Query Start:208 Query End:492 Subject Strand: null Subject Start: 51 Subject End: 146 ............................................................................................................................................................................................................... S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F - F V D S K P I A V C R V A R G K H C K M G R ................................................................................................................................................................................................................................................................................................. ............................................................................................................................................................................................................... S D S E I I T I C I V G E L L T I D S E K A W L G F C K K N M R D L F P K F C D R T R F N R T R R N L H A V I E E I R K E L S N L T G Y A Q Q P Y R I V D S I P I P V C K F G R A K F H K T F R ................................................................................................................................................................................................................................................................................................. Subject: gi|67874253|ref|ZP_00504116.1| Transposase, IS4 [Clostridium thermocellum ATCC 27405] HSP 1 e-value: 1.0E-7 bit: 53.9 Len: 285 Query Start:208 Query End:492 Subject Strand: null Subject Start: 51 Subject End: 146 ............................................................................................................................................................................................................... S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F - F V D S K P I A V C R V A R G K H C K M G R ................................................................................................................................................................................................................................................................................................. ............................................................................................................................................................................................................... S D S E I I T I C I V G E L L T I D S E K A W L G F C K K N M R D L F P K F C D R T R F N R T R R N L H A V I E E I R K E L S N L T G Y A Q Q P Y R I V D S I P I P V C K F G R A K F H K T F R ................................................................................................................................................................................................................................................................................................. Subject: gi|67875175|ref|ZP_00504660.1| Transposase, IS4 [Clostridium thermocellum ATCC 27405] HSP 1 e-value: 1.0E-7 bit: 53.9 Len: 285 Query Start:208 Query End:492 Subject Strand: null Subject Start: 51 Subject End: 146 ............................................................................................................................................................................................................... S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F - F V D S K P I A V C R V A R G K H C K M G R ................................................................................................................................................................................................................................................................................................. ............................................................................................................................................................................................................... S D S E I I T I C I V G E L L T I D S E K A W L G F C K K N M R D L F P K F C D R T R F N R T R R N L H A V I E E I R K E L S N L T G Y A Q Q P Y R I V D S I P I P V C K F G R A K F H K T F R ................................................................................................................................................................................................................................................................................................. Subject: gi|67916593|ref|ZP_00510297.1| Transposase, IS4 [Clostridium thermocellum ATCC 27405] HSP 1 e-value: 1.0E-7 bit: 53.9 Len: 285 Query Start:208 Query End:492 Subject Strand: null Subject Start: 51 Subject End: 146 ............................................................................................................................................................................................................... S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F - F V D S K P I A V C R V A R G K H C K M G R ................................................................................................................................................................................................................................................................................................. ............................................................................................................................................................................................................... S D S E I I T I C I V G E L L T I D S E K A W L G F C K K N M R D L F P K F C D R T R F N R T R R N L H A V I E E I R K E L S N L T G Y A Q Q P Y R I V D S I P I P V C K F G R A K F H K T F R ................................................................................................................................................................................................................................................................................................. Subject: gi|67917102|ref|ZP_00510776.1| Transposase, IS4 [Clostridium thermocellum ATCC 27405] HSP 1 e-value: 1.0E-7 bit: 53.9 Len: 285 Query Start:208 Query End:492 Subject Strand: null Subject Start: 51 Subject End: 146 ............................................................................................................................................................................................................... S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F - F V D S K P I A V C R V A R G K H C K M G R ................................................................................................................................................................................................................................................................................................. ............................................................................................................................................................................................................... S D S E I I T I C I V G E L L T I D S E K A W L G F C K K N M R D L F P K F C D R T R F N R T R R N L H A V I E E I R K E L S N L T G Y A Q Q P Y R I V D S I P I P V C K F G R A K F H K T F R ................................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q4CII1 Cluster: Transposase, IS4; n=3; Clostridium thermocellum ATCC 27405|Rep: Transposase, IS4 - Clostridium thermocellum ATCC 27405 HSP 1 e-value: 1.0E-7 bit: 53.9 Len: 285 Query Start:208 Query End:492 Subject Strand: null Subject Start: 51 Subject End: 146 ............................................................................................................................................................................................................... S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F - F V D S K P I A V C R V A R G K H C K M G R ................................................................................................................................................................................................................................................................................................. ............................................................................................................................................................................................................... S D S E I I T I C I V G E L L T I D S E K A W L G F C K K N M R D L F P K F C D R T R F N R T R R N L H A V I E E I R K E L S N L T G Y A Q Q P Y R I V D S I P I P V C K F G R A K F H K T F R ................................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q9LAJ0 Cluster: Transposase; n=1; Clostridium cellulovorans|Rep: Transposase - Clostridium cellulovorans HSP 1 e-value: 5.0E-7 bit: 51.6 Len: 291 Query Start:208 Query End:498 Subject Strand: null Subject Start: 50 Subject End: 147 ............................................................................................................................................................................................................... S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D - G G E T Q F F V D S K P I A V C R V A R G K H C K M G R R E ........................................................................................................................................................................................................................................................................................... ............................................................................................................................................................................................................... T D S E I I T I S L V G E L L T I D S E K A W F G F C S K N L R D L F P N F C S R P R F H R V R K S L F R V I D E I R K E L T K F L N Y Q Y D R M R I A D S M P I P V C K F G R A H F H K A F K P E ........................................................................................................................................................................................................................................................................................... Subject: gi|93004795|ref|YP_579244.1| transposase, IS4 [Psychrobacter cryohalolentis K5] HSP 1 e-value: 3.0E-6 bit: 49.3 Len: 279 Query Start:190 Query End:468 Subject Strand: null Subject Start: 30 Subject End: 121 ............................................................................................................................................................................................. G P M P K F S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F F V D S K P I A V C R V A R ......................................................................................................................................................................................................................................................................................................................... ............................................................................................................................................................................................. G Y A P K L S D P E V I C M E M V G E F L H L D Q E K Q I W Q Y F T Q H W Q D W F P A I G S Y P N F A K H C A N L W Q V K Q Q I Q D K V - S Q I E G R D N I H F M D G F P I P V C H Y G R ......................................................................................................................................................................................................................................................................................................................... Subject: gi|17228494|ref|NP_485042.1| transposase [Nostoc sp. PCC 7120] HSP 1 e-value: 8.0E-6 bit: 47.8 Len: 306 Query Start:178 Query End:483 Subject Strand: null Subject Start: 28 Subject End: 130 ................................................................................................................................................................................. I P R R G P M P K F S D L E V V A L S L T A E S E S I D S E K W L F D Y K L Q E Y K D K I P N L I S R R Q F N D R R K N T A G L C E D I R K R I A A Q M D G G E T Q F - F V D S K P I A V C R V A R G K H C K .......................................................................................................................................................................................................................................................................................................... ................................................................................................................................................................................. I K R R G F A P S L R E S E V L T M E L V A E F L G I D A E K D I W K Y F H R H W L G L F P Q L K S R Y A F I R Q A A N C W Q Y K E L I Q Q K L A Q Y L G A Y S D Q L H L I D G L P I P L C G F S R A P N C R .......................................................................................................................................................................................................................................................................................................... Find nr database================================================ Query_seq: PI0233:PI0234|PI0233:PI0234:membrane protein, possible exporter:integrase:->->:233832..234612 781 taaaatgtagctataatgatatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaatgtagctataatgatatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: + Start: 86 End: 235 ..................................................................................... M L V Y A T Y T Q N S S K S L R Y A S N S L T T L S I S R E I Y P V V V P C Q N F Q I W R S L P C P .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 58 Strand: + Start: 257 End: 430 ................................................................................................................................................................................................................................................................ M I V K N G F L I I S F R S T K T K Y L T S Y L A G N S T T V E R I P Q D Y A K T Y E R E S L P K W T V V K L N S S ............................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 423 End: 527 ...................................................................................................................................................................................................................................................................................................................................................................................................................................... M L R R F Q A D S R M Q G C Q R E A L Q D G Q T G D C S Q A I W E H L .............................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 13 End: 567 ............ M M I Y K K L H I S L I F S N L L K S K P L S T N P R I C N L Y T K F V K I L E I C K Q F S H N L V N K Q G N I P R R G ...................................................................................................................................................................................................................... Protein_Len: 34 Strand: + Start: 548 End: 649 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M F G N K L L S F L L G T Q T R Y K I V K T R E T K R I Y N A D N Q .................................................................................................................................... Protein_Len: 52 Strand: - Start: 570 End: 725 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... S K V Q Y G S G T C S L S F L L F S F F I Y H Q Y D T I Q T K R M K V L N A W K Q R N K T L Y T C K S M ........................................................ Protein_Len: 34 Strand: - Start: 423 End: 524 ...................................................................................................................................................................................................................................................................................................................................................................................................................................... N K T S E L G I A T H L T A L P F C Q L I P L R S V T G L C N P F M ................................................................................................................................................................................................................................................................. Protein_Len: 53 Strand: - Start: 370 End: 528 ................................................................................................................................................................................................................................................................................................................................................................................. A F V Y S L S D S G L H V T T F S L E E Y I G L R Y G Y A P N G S P L M A L H A S P L S N G P L K P V N M ............................................................................................................................................................................................................................................................. Protein_Len: 58 Strand: - Start: 341 End: 514 .................................................................................................................................................................................................................................................................................................................................................... R G Y F S Y R L I I R L C V F S F R Q G F P R H H F E I R R L N W A S L R I C P Q W L S A N C S P C V P S Q E W A M ........................................................................................................................................................................................................................................................................... Protein_Len: 37 Strand: - Start: 294 End: 404 ..................................................................................................................................................................................................................................................................................................... S Y L S L I G L R M D R L C N L S R L F F V A P N H S S M R F L I A A W M ......................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 36 Strand: - Start: 250 End: 357 ......................................................................................................................................................................................................................................................... L A N I T F F P K K I I L K L L V F V F Y R V E Y R A P L E V V T S L M ........................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 36 Strand: - Start: 164 End: 271 ................................................................................................................................................................... Y A P F Y V G D H D W A L I K L N P P R Q R T R S L P I L S C Q Y H F M .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 35 Strand: - Start: 153 End: 257 ........................................................................................................................................................ L R T L L C P F I G R R P G M G F N E S K S T T A K D K V A S D S L M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 46 Strand: - Start: 3 End: 140 .. F T A I I I Y L F N C M D N I K L L K N F D F G N L V F G R I H L K Y V F N T L I R S I H M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. taaaatgtagctataatgatatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaatgtagctataatgatatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 81 HP_score: -9.2 Tail_Score: -3.89346 Start: 501 End: 523 Full_Region: GGGCAGACGGGAGAC TGTTCCCAGG CAA TTTGGGAACA TTTATGAAATAAGAA ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTTCCCAGGCAATTTGGGAACA.................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 93 HP_score: -9.8 Tail_Score: -4.90266 Start: 543 End: 571 Full_Region: TGAAATAAGAAAAAA GCTAAGTGTTTG GAAA CAAACTACTTAGC TTTTTACTTGGTACC ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GCTAAGTGTTTGGAAACAAACTACTTAGC.................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -9.8 Tail_Score: -5.72254 Start: 543 End: 571 Full_Region: ggtaccaagtaaaaa gctaagtagtttg tttc caaacacttagc ttttttcttatttca ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gctaagtagtttgtttccaaacacttagc.................................................................................................................................................................................................................. Find igs database================================================ Query_seq: PI0233:PI0234|PI0233:PI0234:membrane protein, possible exporter:integrase:->->:233832..234612 781 taaaatgtagctataatgatatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagtaatttgagtttttctcatttttaccaaattagcccatttttgcctgttttttgttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Intra-Species Hit: Count: 1 Min: 503 Max: 781 Len: 279 Subject: pint_PI0233_PI0234|membrane protein, possible exporter:integrase|POSITIVE:POSITIVE|[233832,234612]|781 HSP 1 e-value: 1.0E-78 bit: 295.0 Len: 149 Query Start:503 Query End:651 Subject Strand: POSITIVE Subject Start: 503 Subject End: 651 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagta.................................................................................................................................. ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttcccaggcaatttgggaacatttatgaaataagaaaaaagctaagtgtttggaaacaaactacttagctttttacttggtacccagacccggtacaagatagtgaaaacaagagaaacgaaaagaatatataatgctgataatcagta.................................................................................................................................. HSP 2 e-value: 7.0E-37 bit: 157.0 Len: 79 Query Start:703 Query End:781 Subject Strand: POSITIVE Subject Start: 703 Subject End: 781 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gttagataagtacactttgatattcaagactttttagtatctttgcaaaataagaaaacacctgaaaataaacgagtca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCCCAGGCAAUUUGGGAACAUUUAUGAAAUAAGAAAAAAGCUAAGUGUUUGGAAACAAACUACUUAGCUUUUUACUUGGUACCCAGACCCGGUACAAGAUAGUGAAAACAAGAGAAACGAAAAGAAUAUAUAAUGCUGAUAAUCAGUAAUUUGAGUUUUUCUCAUUUUUACCAAAUUAGCCCAUUUUUGCCUGUUUUUUGUUAGAUAAGUACACUUUGAUAUUCAAGACUUUUUAGUAUCUUUGCAAAAUAAGAAAACACCUGAAAAUAAACGAGUCA ....(((((((..((((...((((......((((.(((((((((((((((((....))))).)))))))))))).))))(((((.......)))))......(((((((..((((((((..(((..........(((((.....))))).)))..))))))))..))))))).))))...))))...)))))))..((((((.(((((.......((((.....)))).........)))))..))))))............................. (-72.19) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACUCGUUUAUUUUCAGGUGUUUUCUUAUUUUGCAAAGAUACUAAAAAGUCUUGAAUAUCAAAGUGUACUUAUCUAACAAAAAACAGGCAAAAAUGGGCUAAUUUGGUAAAAAUGAGAAAAACUCAAAUUACUGAUUAUCAGCAUUAUAUAUUCUUUUCGUUUCUCUUGUUUUCACUAUCUUGUACCGGGUCUGGGUACCAAGUAAAAAGCUAAGUAGUUUGUUUCCAAACACUUAGCUUUUUUCUUAUUUCAUAAAUGUUCCCAAAUUGCCUGGGAA ..((.(.......((((((..((((..(((((.....)))))..))))...)))))).......).)).................(((((((...((((......((((.((((..(((.((((........(((.....))).((.....)).......)))).)))..)))).))))....(((((.......))))).(((.((((((((((((.(((((....))))))))))))))))).)))..............))))..))))))).... (-59.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 119 78 145 100 pint:PI1490|5end_GTP_cyclohydrolase_I_1408571..1408801_POSITIVE 241 50 5 92 51 pint:PI1458|5end_dihydroneopterin_aldolase_1381166..1381396_POSITIVE 237 200 154 45 3 pint:PI1496|5end_nucleoside_phosphorylase_1417116..1417346_POSITIVE 237 116 84 114 82 pint:PI0314|5end_conserved_hypothetical_protein_295552..295782_POSITIVE 235 130 85 112 67 pint:PI0280|5end_conserved_hypothetical_protein_266001..266231_POSITIVE 234 196 151 113 71 pint:PI1825|5end_2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase_1764996..1765226_POSITIVE 233 99 60 130 92 pint:PI0985|5end_aspartyl-tRNA_synthetase_910174..910404_POSITIVE 233 220 180 150 104 pint:PI0388|5end_integrase_354927..355157_POSITIVE 233 240 196 196 144 pint:PI0053|5end_hypothetical_protein_54727..54957_POSITIVE 232 100 52 97 56 pint:PI1899|5end_conserved_hypothetical_protein_1842231..1842461_POSITIVE 230 110 64 220 162 pint:PI1532|5end_uridylate_kinase_1451644..1451874_POSITIVE 227 94 64 63 26 pint:PI1778|5end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1710009..1710239_POSITIVE 227 99 57 85 47 pint:PI0660|5end_conserved_hypothetical_protein_603438..603668_POSITIVE 227 225 184 123 81 pint:PI0290|5end_conserved_hypothetical_protein_273245..273475_POSITIVE 226 93 52 227 184 pint:PI1646|5end_hypothetical_protein_1561954..1562184_POSITIVE 225 83 44 146 111 pint:PI1989|5end_conserved_hypothetical_protein;_possible_thioredoxin_1944018..1944248_POSITIVE 223 104 64 44 2 pint:PI0727|5end_integrase_659261..659491_POSITIVE 223 108 62 88 28 pint:PI0288|5end_hypothetical_protein_(possible_ISPg3-related_transposase)_272272..272502_POSITIVE 222 226 186 216 169 pint:PI0140|5end_conserved_hypothetical_protein_131643..131873_POSITIVE 222 118 76 172 124 pint:PI0125|5end_undecaprenyl_pyrophosphate_synthase_117106..117336_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 252 92 68 151 125 pint:PI0601|3end_hypothetical_protein_549020..549170_POSITIVE 249 96 61 128 88 pint:PI2137|3end_RNA_methylase_SpoU_family_2083807..2083957_POSITIVE 247 119 78 81 36 pint:PI1489|3end_exodeoxyribonuclease_III_1408587..1408737_POSITIVE 247 257 211 138 84 pint:PI0611|3end_hypothetical_protein_559901..560051_POSITIVE 241 50 5 74 33 pint:PI1457|3end_N-acetylmuramoyl-L-alanine_amidase_1381228..1381378_POSITIVE 235 130 85 112 67 pint:PI0279|3end_hypothetical_protein_266081..266231_POSITIVE 227 94 64 55 18 pint:PI1777|3end_conserved_hypothetical_protein_1710081..1710231_POSITIVE 227 94 51 123 78 pint:PI0444|3end_50S_ribosomal_protein_L7/L12_413390..413540_POSITIVE 227 94 51 92 47 pint:PI0445|3end_conserved_hypothetical_protein_413359..413509_POSITIVE 227 225 184 48 6 pint:PI0289|3end_hypothetical_protein_273250..273400_POSITIVE 225 83 44 116 81 pint:PI1988|3end_conserved_hypothetical_protein_1944068..1944218_POSITIVE 225 196 155 42 7 pint:PI1824|3end_ThiJ/PfpI_family_protein_1765005..1765155_POSITIVE 225 208 161 73 25 pint:PI1610|3end_Na+/H+_anti-porter_1538002..1538152_POSITIVE 223 129 85 86 27 pint:PI1166|3end_hypothetical_protein_1089537..1089687_POSITIVE 223 108 62 88 28 pint:PI0287|3end_ISPg3-related_transposase_272352..272502_POSITIVE 221 55 13 58 3 pint:PI2167|3end_hypothetical_protein_2111891..2112041_POSITIVE 221 204 181 90 70 pint:PI1770|3end_4-alpha-glucanotransferase_1699998..1700148_POSITIVE 221 140 91 117 74 pint:PI1422|3end_hypothetical_protein_1341942..1342092_POSITIVE 221 149 102 106 58 pint:PI0717|3end_conserved_hypothetical_protein_653120..653270_POSITIVE 217 176 135 118 70 pint:PI0999|3end_hypothetical_protein_922812..922962_POSITIVE