Origin IGS: attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atggcttaaaaatcagaactgactgactcacaattcatttacgctattttgtcttttcttgaaccttgaggatagatttttatctaaaacgatagtactttttggacgaaaagtactatcgttttttctgaaaggtaataatattctttttaaaagctatataagaatagagaaaaacgatagtactttccaacttttatgaagtcttgataagccatatctatttatttcgttgaagcgaaatatgacaat Mask Tandem Repeat Region ================================================ attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Find is-nt database================================================ Query_seq: PI0214:PI0215|PI0214:PI0215:hypothetical protein:hypothetical protein:->->:208711..208962 252 attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0214:PI0215|PI0214:PI0215:hypothetical protein:hypothetical protein:->->:208711..208962 252 attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0214:PI0215|PI0214:PI0215:hypothetical protein:hypothetical protein:->->:208711..208962 252 attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 1 End: 93 M V I F R F N E I N R Y G L S R L H K S W K V L S F F S I L I ............................................................................................................................................................... Protein_Len: 38 Strand: + Start: 109 End: 222 ............................................................................................................ M L L P F R K N D S T F R P K S T I V L D K N L S S R F K K R Q N S V N E L .............................. Protein_Len: 59 Strand: - Start: 54 End: 230 ..................................................... L L Q F T S D N K E I R I Y S K F L I N N G K L F F S L V K R G F L V I T K S L F R D E L N L F L C F L T F S N H T M ...................... attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -14.0 Tail_Score: -5.24531 Start: 132 End: 163 Full_Region: gatttttatctaaaa cgatagtacttttt ggac gaaaagtactatcg ttttttctgaaaggt ...................................................................................................................................cgatagtactttttggacgaaaagtactatcg......................................................................................... Find igs database================================================ Query_seq: PI0214:PI0215|PI0214:PI0215:hypothetical protein:hypothetical protein:->->:208711..208962 252 attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Intra-Species Hit: Count: 1 Min: 1 Max: 252 Len: 252 Subject: pint_PI0214_PI0215|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[208711,208962]|252 HSP 1 e-value: 1.0E-140 bit: 500.0 Len: 252 Query Start:1 Query End:252 Subject Strand: POSITIVE Subject Start: 1 Subject End: 252 attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat attgtcatatttcgcttcaacgaaataaatagatatggcttatcaagacttcataaaagttggaaagtactatcgtttttctctattcttatatagcttttaaaaagaatattattacctttcagaaaaaacgatagtacttttcgtccaaaaagtactatcgttttagataaaaatctatcctcaaggttcaagaaaagacaaaatagcgtaaatgaattgtgagtcagtcagttctgatttttaagccat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGUCAUAUUUCGCUUCAACGAAAUAAAUAGAUAUGGCUUAUCAAGACUUCAUAAAAGUUGGAAAGUACUAUCGUUUUUCUCUAUUCUUAUAUAGCUUUUAAAAAGAAUAUUAUUACCUUUCAGAAAAAACGAUAGUACUUUUCGUCCAAAAAGUACUAUCGUUUUAGAUAAAAAUCUAUCCUCAAGGUUCAAGAAAAGACAAAAUAGCGUAAAUGAAUUGUGAGUCAGUCAGUUCUGAUUUUUAAGCCAU .......(((((((......))))))).......((((((((......(((..((((((((((((((.((....))))))))............))))))))..)))..............((((((.((((((((((((((((......))))))))))))))))(((((....)))))..((((..(((((.......((........))...)))))..))))........))))))....)))))))) (-55.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGGCUUAAAAAUCAGAACUGACUGACUCACAAUUCAUUUACGCUAUUUUGUCUUUUCUUGAACCUUGAGGAUAGAUUUUUAUCUAAAACGAUAGUACUUUUUGGACGAAAAGUACUAUCGUUUUUUCUGAAAGGUAAUAAUAUUCUUUUUAAAAGCUAUAUAAGAAUAGAGAAAAACGAUAGUACUUUCCAACUUUUAUGAAGUCUUGAUAAGCCAUAUCUAUUUAUUUCGUUGAAGCGAAAUAUGACAAU ((((((((....((((......))))....(((.........((((((..((.(((((((..(((((..(((.((((....))))((((((((((((((((((....)))))))))))))))))).)))..))))).....(((((((..............))))))))))))))))))))))........(((((...))))).))).)))))))).......((((((((....))))))))....... (-61.04) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 248 41 4 221 183 pint:PI1986|5end_hypothetical_protein_1940863..1941093_POSITIVE 235 150 111 186 138 pint:PI1475|5end_possible_short_chain_dehydrogenase_1397142..1397372_POSITIVE 233 163 122 178 139 pint:PI1380|5end_conserved_hypothetical_protein_1302587..1302817_POSITIVE 230 77 37 209 172 pint:PI0637|5end_hypothetical_protein_582117..582347_POSITIVE 230 77 37 124 87 pint:PI0636|5end_hypothetical_protein_582032..582262_POSITIVE 221 250 209 40 3 pint:PI0753|5end_hypothetical_protein_683391..683621_POSITIVE 220 166 124 70 27 pint:PI0550|5end_possible_glycosyl_hydrolase_500650..500880_POSITIVE 217 40 1 64 26 pint:PI1927|5end_hypothetical_protein_1871187..1871417_POSITIVE 211 48 10 127 95 pint:PI1542|5end_mobilization_protein_1461728..1461958_POSITIVE 211 250 201 231 169 pint:PI0074|5end_Na+/phosphate_symporter_(sodium-dependent_inorganic_phosphate_(Pi)_transporter)_71853..72083_POSITIVE 209 90 46 82 28 pint:PI1214|5end_peptide_chain_release_factor_1_1132631..1132861_POSITIVE 208 69 29 101 54 pint:PI1342|5end_conserved_hypothetical_protein_1257057..1257287_POSITIVE 208 49 14 114 79 pint:PI1006|5end_conserved_hypothetical_protein_926604..926834_POSITIVE 206 101 61 74 37 pint:PI0511|5end_hypothetical_protein_470434..470664_POSITIVE 204 163 123 67 21 pint:PI1720|5end_conserved_hypothetical_protein_1636146..1636376_POSITIVE 204 233 192 67 16 pint:PI0161|5end_possible_TonB_receptor_153364..153594_POSITIVE 203 228 185 59 19 pint:PI0113|5end_arginyl-tRNA_synthetase_108371..108601_POSITIVE 202 229 184 100 54 pint:PI1036|5end_ATP-dependent__Lon_protease_954446..954676_POSITIVE 202 88 47 110 73 pint:PI0915|5end_hypothetical_protein_845183..845413_POSITIVE 202 250 210 167 119 pint:PI0676|5end_hypothetical_protein_614576..614806_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 248 41 4 63 25 pint:PI1984|3end_MutT/nudix_family_protein_1940785..1940935_POSITIVE 227 146 105 144 99 pint:PI0653|3end_hypothetical_protein_596236..596386_POSITIVE 226 234 200 78 50 pint:PI0132|3end_hypothetical_protein_124519..124669_POSITIVE 226 234 200 48 20 pint:PI0131|3end_conserved_hypothetical_protein_(possible_outer_membrane_lipoprotein)_124489..124639_POSITIVE 219 240 196 63 19 pint:PI0191|3end_O-methyltransferase_181785..181935_POSITIVE 216 241 205 73 26 pint:PI0425|3end_hypothetical_protein_389722..389872_POSITIVE 212 250 201 94 27 pint:PI0447|3end_DNA-directed_RNA_polymerase,_beta'_subunit_421755..421905_POSITIVE 211 48 10 90 58 pint:PI1540|3end_hypothetical_protein_1461771..1461921_POSITIVE 211 145 124 29 7 pint:PI0214|3end_hypothetical_protein_208650..208800_POSITIVE 211 250 201 83 21 pint:PI0073|3end_dihydropteroate_synthase_71785..71935_POSITIVE 210 225 185 81 39 pint:PI1724|3end_inosine-5'-monophosphate_dehydrogenase_1642666..1642816_POSITIVE 209 90 46 58 4 pint:PI1213|3end_phosphoribosylformylglycinamidine_cyclo-ligase_(AIR_synthase)_1132687..1132837_POSITIVE 208 69 29 128 81 pint:PI1339|3end_hypothetical_protein_1257164..1257314_POSITIVE 204 163 123 48 2 pint:PI1719|3end_conserved_hypothetical_protein_1636207..1636357_POSITIVE 203 228 185 135 95 pint:PI0114|3end_hypothetical_protein_108527..108677_POSITIVE 202 229 184 107 61 pint:PI1035|3end_queuine_tRNA-ribosyltransferase_(tRNA-guanine_transglycosylase)_954533..954683_POSITIVE 202 166 125 69 19 pint:PI1005|3end_hypothetical_protein_926481..926631_POSITIVE 201 65 22 82 26 pint:PI0782|3end_beta-lactamase_induction_signal_transducer,_AmpG_protein_721927..722077_POSITIVE 198 54 14 74 25 pint:PI0494|3end_hypothetical_protein_455170..455320_POSITIVE 197 239 193 109 72 pint:PI0303|3end_hypothetical_protein_287610..287760_POSITIVE