Origin IGS: taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagcgattgtataaccacctcgttaccagcgtattacaaaacctattgttttgcattccaaaagcgtaggttttgcacggtaaaagcggctgttttgcgatgcaaaacagccgctttcgcaatgccaaatcgaaattgtcattttctctacgaattatttttacagaacgagggctttcttctcaagcaataacaagattttttactgcaaaagaaaagagggtgtatcaaaacttagagttctgatacatcctcttttattctatcttgttggaagtcttttaaacctccgtttattcttgtttcggctta Mask Tandem Repeat Region ================================================ taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Find is-nt database================================================ Query_seq: PI0195:PI0196|PI0195:PI0196:epimerase/reductase:hypothetical protein:->->:188263..188574 312 taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0195:PI0196|PI0195:PI0196:epimerase/reductase:hypothetical protein:->->:188263..188574 312 taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0195:PI0196|PI0195:PI0196:epimerase/reductase:hypothetical protein:->->:188263..188574 312 taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 171 End: 287 .......................................................................................................................................................................... M T I S I W H C E S G C F A S Q N S R F Y R A K P T L L E C K T I G F V I R W ......................... Protein_Len: 52 Strand: + Start: 155 End: 310 .......................................................................................................................................................... M I R R E N D N F D L A L R K R L F C I A K Q P L L P C K T Y A F G M Q N N R F C N T L V T R W L Y N R .. Protein_Len: 60 Strand: - Start: 123 End: 302 .......................................................................................................................... Q K L L F G E N Q L F L E Y L F H C N R N P M A F A A T K C R L V A A K V T C F R R K Q F A F C Y T K Y Y A P L S T T M .......... Protein_Len: 57 Strand: - Start: 70 End: 240 ..................................................................... F E L N Q Y V R K E K A T F F R T I A Q S S L G R E T F I I R L S F S L K S K A N R F R S N Q M A F C G S K G H M ........................................................................ Protein_Len: 60 Strand: - Start: 2 End: 223 . L Y F L L P H I L V R L K S V G E K R K C Y F I K N N S S F F A R T R Y F Y N T S F I V I E I Q C Q S L P Q K A D C F M ......................................................................................... taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -16.2 Tail_Score: -4.13767 Start: 53 End: 99 Full_Region: TCCAACAAGATAGAA TAAAAGAGGATGTATCAGAACT CTA AGTTTTGATACACCCTCTTTTC TTTTGCAGTAAAAAA ....................................................TAAAAGAGGATGTATCAGAACTCTAAGTTTTGATACACCCTCTTTTC..................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -16.2 Tail_Score: -3.76835 Start: 53 End: 99 Full_Region: ttttttactgcaaaa gaaaagagggtgtatcaaaact tag agttctgatacatcctctttta ttctatcttgttgga ....................................................gaaaagagggtgtatcaaaacttagagttctgatacatcctctttta..................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -16.1 Tail_Score: -5.09259 Start: 58 End: 94 Full_Region: CAAGATAGAATAAAA GAGGATGTATCAGAACT CTA AGTTTTGATACACCCTC TTTTCTTTTGCAGTA .........................................................GAGGATGTATCAGAACTCTAAGTTTTGATACACCCTC.......................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -16.1 Tail_Score: -4.90002 Start: 58 End: 94 Full_Region: tactgcaaaagaaaa gagggtgtatcaaaact tag agttctgatacatcctc ttttattctatcttg .........................................................gagggtgtatcaaaacttagagttctgatacatcctc.......................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -21.9 Tail_Score: -4.13767 Start: 199 End: 227 Full_Region: TTTGGCATTGCGAAA GCGGCTGTTTTGC ATC GCAAAACAGCCGC TTTTACCGTGCAAAA ......................................................................................................................................................................................................GCGGCTGTTTTGCATCGCAAAACAGCCGC..................................................................................... TransTerm Strand: - Conf: 100 HP_score: -21.9 Tail_Score: -3.57144 Start: 199 End: 227 Full_Region: ttttgcacggtaaaa gcggctgttttgc gat gcaaaacagccgc tttcgcaatgccaaa ......................................................................................................................................................................................................gcggctgttttgcgatgcaaaacagccgc..................................................................................... Find igs database================================================ Query_seq: PI0195:PI0196|PI0195:PI0196:epimerase/reductase:hypothetical protein:->->:188263..188574 312 taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Intra-Species Hit: Count: 35 Min: 1 Max: 312 Len: 312 Subject: pint_PI0195_PI0196|epimerase/reductase:hypothetical protein|POSITIVE:POSITIVE|[188263,188574]|312 HSP 1 e-value: 1.0E-176 bit: 618.0 Len: 312 Query Start:1 Query End:312 Subject Strand: POSITIVE Subject Start: 1 Subject End: 312 taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta taagccgaaacaagaataaacggaggtttaaaagacttccaacaagatagaataaaagaggatgtatcagaactctaagttttgatacaccctcttttcttttgcagtaaaaaatcttgttattgcttgagaagaaagccctcgttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatacaatcgcta Subject: pint_PI1391_PI1392|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1311755,1312040]|286 HSP 1 e-value: 2.0E-47 bit: 190.0 Len: 104 Query Start:176 Query End:279 Subject Strand: NEGATIVE Subject Start: 53 Subject End: 156 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa................................. ...............................................................................................................................................................................aatttcgatttggcgttgcgaaagcggctgttttgcagcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa................................. Subject: pint_PI0948_PI0949|hypothetical protein:endonuclease|POSITIVE:POSITIVE|[882039, 882403]|365 HSP 1 e-value: 3.0E-46 bit: 186.0 Len: 122 Query Start:155 Query End:276 Subject Strand: POSITIVE Subject Start: 151 Subject End: 272 ..........................................................................................................................................................ataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttg.................................... ..........................................................................................................................................................ataattcgtaaggaaaatgataatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgctttgaccgtgtaaaacctacgcttttacaatgcaaaacaataggttttg.................................... Subject: pint_PI1424_PI1425|probable peptidase family M48:conserved hypothetical protein|POSITIVE:POSITIVE|[1344105,1344627]|523 HSP 1 e-value: 1.0E-45 bit: 184.0 Len: 109 Query Start:168 Query End:276 Subject Strand: NEGATIVE Subject Start: 106 Subject End: 214 .......................................................................................................................................................................aaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttg.................................... .......................................................................................................................................................................aaaatgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacagccgcttttgccgtgtaaaacctacgcttttggaatgcaaaacaataggttttg.................................... HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:196 Query End:230 Subject Strand: POSITIVE Subject Start: 152 Subject End: 186 ...................................................................................................................................................................................................aaagcggctgttttgcatcgcaaaacagccgcttt.................................................................................. ...................................................................................................................................................................................................aaagcggctgttttgcgttgcaaaacagccgcttt.................................................................................. Subject: pint_PI0854_PI0855|MiaB-like tRNA modifying enzyme:hypothetical protein|POSITIVE:POSITIVE|[788060,789233]|1174 HSP 1 e-value: 2.0E-41 bit: 170.0 Len: 114 Query Start:168 Query End:281 Subject Strand: POSITIVE Subject Start: 764 Subject End: 877 .......................................................................................................................................................................aaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaata............................... .......................................................................................................................................................................aaaatgataatttcgatttggcgttgcgaaagcgtaggttttgcaacgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttataata............................... Subject: pint_PI0962_PI0963|phosphoglycerate mutase:hypothetical protein|POSITIVE:POSITIVE|[892763,893337]|575 HSP 1 e-value: 7.0E-41 bit: 168.0 Len: 129 Query Start:148 Query End:276 Subject Strand: POSITIVE Subject Start: 147 Subject End: 275 ...................................................................................................................................................tgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttg.................................... ...................................................................................................................................................tgtaaagataattcgttgaaaaaatgataatttcgatttgacattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttg.................................... Subject: pint_PI1981_PI1982|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1938199,1938578]|380 HSP 1 e-value: 1.0E-39 bit: 165.0 Len: 107 Query Start:175 Query End:281 Subject Strand: POSITIVE Subject Start: 174 Subject End: 280 ..............................................................................................................................................................................caatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaata............................... ..............................................................................................................................................................................caatttcggtttgacattgcgaaagcggctgttttgcatttcaaaacagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggttttataata............................... Subject: pint_PI1958_PI1959|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1917430,1917847]|418 HSP 1 e-value: 1.0E-39 bit: 165.0 Len: 107 Query Start:175 Query End:281 Subject Strand: POSITIVE Subject Start: 212 Subject End: 318 ..............................................................................................................................................................................caatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaata............................... ..............................................................................................................................................................................caatttcggtttgacattgcaaaagcggctgttttgcattgcaaaacagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggttttataata............................... HSP 2 e-value: 7.0E-7 bit: 56.0 Len: 36 Query Start:196 Query End:231 Subject Strand: NEGATIVE Subject Start: 232 Subject End: 267 ...................................................................................................................................................................................................aaagcggctgttttgcatcgcaaaacagccgctttt................................................................................. ...................................................................................................................................................................................................aaagcggctgttttgcaatgcaaaacagccgctttt................................................................................. Subject: pint_PI0145_PI0146|secDF export membrane protein:transcription elongation factor|POSITIVE:POSITIVE|[139785,140477]|693 HSP 1 e-value: 3.0E-37 bit: 157.0 Len: 107 Query Start:176 Query End:282 Subject Strand: NEGATIVE Subject Start: 226 Subject End: 332 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatac.............................. ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcgtaggttttgcaatgcaaaacagccgcttttaccgtgcaaaacctacacttttggaatgcaaaacaataggttttataatac.............................. Subject: pint_PI1688_PI1689|possible GTPase:conserved hypothetical protein|POSITIVE:POSITIVE|[1603964,1604449]|486 HSP 1 e-value: 7.0E-35 bit: 149.0 Len: 139 Query Start:148 Query End:286 Subject Strand: NEGATIVE Subject Start: 163 Subject End: 301 ...................................................................................................................................................tgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctg.......................... ...................................................................................................................................................tgtaaagataattcggagggaaaatgataatttcgatttgacgttgcgaaagcgtatgttttgcgatgcaaaagagccgcttttaccgtgcaaaacctacgcttttacaatgcaaaacaataggttttataacacgctg.......................... Subject: pint_PI0210_PI0211|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[203859,204206]|348 HSP 1 e-value: 3.0E-34 bit: 147.0 Len: 106 Query Start:184 Query End:289 Subject Strand: POSITIVE Subject Start: 240 Subject End: 344 .......................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggta....................... .......................................................................................................................................................................................tttggcgttgcgaaagcggctgtttgcgat-gcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatgcgttggta....................... HSP 2 e-value: 3.0E-6 bit: 54.0 Len: 35 Query Start:196 Query End:230 Subject Strand: NEGATIVE Subject Start: 252 Subject End: 285 ...................................................................................................................................................................................................aaagcggctgttttgcatcgcaaaacagccgcttt.................................................................................. ...................................................................................................................................................................................................aaagcggctgttttgcatcgc-aaacagccgcttt.................................................................................. Subject: pint_PI1563_PI1564|thiol:disulfide interchange protein dsbD:surface antigen BspA|POSITIVE:POSITIVE|[1482876,1483594]|719 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 113 Query Start:168 Query End:280 Subject Strand: NEGATIVE Subject Start: 382 Subject End: 494 .......................................................................................................................................................................aaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaat................................ .......................................................................................................................................................................aaaatgataatttcgatttggcattgcgaaagcggctgttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttgtaat................................ Subject: pint_PI0023_PI0024|internalin-related protein:hypothetical protein|POSITIVE:POSITIVE|[28131,28517]|387 HSP 1 e-value: 4.0E-33 bit: 143.0 Len: 100 Query Start:176 Query End:275 Subject Strand: POSITIVE Subject Start: 246 Subject End: 345 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt..................................... ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt..................................... Subject: pint_PI2088_PI2089|conserved hypothetical protein; possible PAP2 superfamily protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2045876,2046339]|464 HSP 1 e-value: 3.0E-31 bit: 137.0 Len: 133 Query Start:148 Query End:280 Subject Strand: POSITIVE Subject Start: 158 Subject End: 290 ...................................................................................................................................................tgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaat................................ ...................................................................................................................................................tgtaaagataattcgttaagaaaacgataatttcgatttgacgttgcgaaagctgctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttataat................................ Subject: pint_PI1441_PI1442|hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme)|POSITIVE:POSITIVE|[1363734,1364088]|355 HSP 1 e-value: 2.0E-29 bit: 131.0 Len: 102 Query Start:178 Query End:279 Subject Strand: NEGATIVE Subject Start: 82 Subject End: 183 .................................................................................................................................................................................tttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa................................. .................................................................................................................................................................................tttcgatttgacgttgcgaaagcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaacgcaaaacaataggttttgtaa................................. Subject: pint_PI1332_PI1333|aminotransferase, possible pyridoxal-phosphate-dependent aminotransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[1250627,1250916]|290 HSP 1 e-value: 2.0E-28 bit: 127.0 Len: 108 Query Start:176 Query End:283 Subject Strand: POSITIVE Subject Start: 122 Subject End: 229 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacg............................. ...............................................................................................................................................................................aatttcgatttgacattgcgaaaacggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggagtgcaaaacaataggttttgcaatacg............................. Subject: pint_PI0990_PI0991|GDP-mannose 4,6-dehydratase:conserved hypothetical protein|POSITIVE:POSITIVE|[915115,915448]|334 HSP 1 e-value: 1.0E-27 bit: 125.0 Len: 103 Query Start:184 Query End:286 Subject Strand: POSITIVE Subject Start: 207 Subject End: 309 .......................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctg.......................... .......................................................................................................................................................................................tttggcattgcgaaagcggctcttttgcattgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggttttgtaatgcgctg.......................... Subject: pint_PI0574_PI0575|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[519505,519700]|196 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 113 Query Start:168 Query End:280 Subject Strand: POSITIVE Subject Start: 33 Subject End: 145 .......................................................................................................................................................................aaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaat................................ .......................................................................................................................................................................aaaatgataatttcgatttgacgttgcgaaagtggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttgcaatgcaaaacaataggttttgtaat................................ Subject: pint_PI1803_PI1804|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1737852,1738248]|397 HSP 1 e-value: 6.0E-26 bit: 119.0 Len: 100 Query Start:176 Query End:275 Subject Strand: POSITIVE Subject Start: 297 Subject End: 396 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt..................................... ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttt..................................... Subject: pint_PI1485_PI1486|hypothetical protein:alginate O-acetylation protein|POSITIVE:POSITIVE|[1405400,1405646]|247 HSP 1 e-value: 2.0E-25 bit: 117.0 Len: 103 Query Start:184 Query End:286 Subject Strand: NEGATIVE Subject Start: 87 Subject End: 189 .......................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctg.......................... .......................................................................................................................................................................................tttgacattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaacgcaaaacaataggttttgcaatacgctg.......................... Subject: pint_PI0874_PI0875|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[810342,810747]|406 HSP 1 e-value: 2.0E-25 bit: 117.0 Len: 103 Query Start:184 Query End:286 Subject Strand: NEGATIVE Subject Start: 180 Subject End: 282 .......................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctg.......................... .......................................................................................................................................................................................tttggcattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaacacgttttataacacgctg.......................... Subject: pint_PI0051_PI0054|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[53449,54718]|1270 HSP 1 e-value: 2.0E-25 bit: 117.0 Len: 111 Query Start:166 Query End:276 Subject Strand: NEGATIVE Subject Start: 87 Subject End: 197 .....................................................................................................................................................................agaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttg.................................... .....................................................................................................................................................................agaaaatggtaatttcgatttgacattgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttg.................................... Subject: pint_PI2056_PI2057|conserved hypothetical protein:conserved hypothetical protein; possible metal-dependent membrane protease|POSITIVE:POSITIVE|[2014489,2015078]|590 HSP 1 e-value: 4.0E-24 bit: 113.0 Len: 141 Query Start:148 Query End:288 Subject Strand: POSITIVE Subject Start: 344 Subject End: 484 ...................................................................................................................................................tgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggt........................ ...................................................................................................................................................tgtaaagataattcgttaagaaaacaataatttcgatttgacgttgcgaaagcggctcttttgtaatgcaaaacttacgcttttaccatgcaaaacagccgcttttggaatgcgaaacaataggttttgtaatacactggt........................ Subject: pint_PI1090_PI1091|tRNA uracil 5-methyltransferase:transcriptional regulator, AsnC family|POSITIVE:POSITIVE|[1015841,1016319]|479 HSP 1 e-value: 4.0E-24 bit: 113.0 Len: 121 Query Start:184 Query End:304 Subject Strand: POSITIVE Subject Start: 150 Subject End: 270 .......................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacgaggtggttatac........ .......................................................................................................................................................................................tttgacattgcaaaagcagctgttttacgatgcaaaacaaccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggttttataacacactgatgatgaggtagttatac........ Subject: pint_PI0694_PI0695|Na+/dicarboxylate or sulfate symporter:xanthine/uracil permease family protein|POSITIVE:POSITIVE|[631238,631552]|315 HSP 1 e-value: 4.0E-24 bit: 113.0 Len: 101 Query Start:176 Query End:276 Subject Strand: POSITIVE Subject Start: 119 Subject End: 219 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttg.................................... ...............................................................................................................................................................................aatttcgatttgacactgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaatgcaaaacaataggttttg.................................... Subject: pint_PI2051_PI2052|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2007896,2008733]|838 HSP 1 e-value: 1.0E-23 bit: 111.0 Len: 132 Query Start:148 Query End:279 Subject Strand: NEGATIVE Subject Start: 328 Subject End: 459 ...................................................................................................................................................tgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa................................. ...................................................................................................................................................tgtaaagataattcgttaagaaaacgataatttcgatttgacgttgcgaaagcagctcttttgcaacgcaaaacctatgcttttaccgtgcaaaacagccgcttttaggatgcaaaacaataggttttgtaa................................. Subject: pint_PI1577_PI1578|conserved hypothetical protein:rod shape-determining protein|POSITIVE:POSITIVE|[1503940,1504327]|388 HSP 1 e-value: 6.0E-23 bit: 109.0 Len: 131 Query Start:145 Query End:275 Subject Strand: POSITIVE Subject Start: 69 Subject End: 199 ................................................................................................................................................ttctgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt..................................... ................................................................................................................................................ttctgtaaagataattcattaagaaaactataatttcgatttgacgttgcgaaagcggctcttttgcgctgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt..................................... Subject: pint_PI0141_PI0142|glutamine amidotransferase involved in pyridoxine biosynthesis:DNA-binding protein|POSITIVE:POSITIVE|[133369,133978]|610 HSP 1 e-value: 6.0E-23 bit: 109.0 Len: 103 Query Start:184 Query End:286 Subject Strand: POSITIVE Subject Start: 205 Subject End: 307 .......................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctg.......................... .......................................................................................................................................................................................tttgacattgcgaaagcggctgttttgcgctgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggttttgtaatgcgctg.......................... Subject: pint_PI1930_PI1931|conserved hypothetical protein; possible ankyrin repeat protein (eukaryotic-like):alpha glycan phosphorylase, maltodextrin phosphorylase|POSITIVE:POSITIVE|[1875115,1875595]|481 HSP 1 e-value: 9.0E-22 bit: 105.0 Len: 105 Query Start:176 Query End:280 Subject Strand: POSITIVE Subject Start: 144 Subject End: 248 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaat................................ ...............................................................................................................................................................................aatttcgatttgacgttgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggttttgtaat................................ Subject: pint_PI0932_PI0933|conserved hypothetical protein; possible hemin receptor:hypothetical protein|POSITIVE:POSITIVE|[865425,865839]|415 HSP 1 e-value: 9.0E-22 bit: 105.0 Len: 101 Query Start:175 Query End:275 Subject Strand: NEGATIVE Subject Start: 179 Subject End: 279 ..............................................................................................................................................................................caatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt..................................... ..............................................................................................................................................................................caattgcgttttgacattgcaaaagcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaacgcgaaacaataggtttt..................................... Subject: pint_PI1051_PI1052|hypothetical protein:fructose-1, 6-bisphosphatase|POSITIVE:POSITIVE|[970289,970631]|343 HSP 1 e-value: 4.0E-21 bit: 103.0 Len: 104 Query Start:176 Query End:279 Subject Strand: NEGATIVE Subject Start: 37 Subject End: 140 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa................................. ...............................................................................................................................................................................aatttcgttttgacgttgcgaaagcggctcttttacaacgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaatgcaaaacaataggttttgtaa................................. Subject: pint_PI1583_PI1584|phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR)/IMP cyclohydrolase (ATIC):A/G-specific adenine glycosylase|POSITIVE:POSITIVE|[1511261,1512037]|777 HSP 1 e-value: 9.0E-19 bit: 95.6 Len: 104 Query Start:176 Query End:279 Subject Strand: NEGATIVE Subject Start: 407 Subject End: 510 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaa................................. ...............................................................................................................................................................................aatttcgttttgacgttgcgaaagcggctgttttgcgaggtaaaacctacgcttttgccgtgcaaaacagccgcttttggaatgcaaaacaataggttttgtaa................................. Subject: pint_PI1915_PI1916|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1859967,1860350]|384 HSP 1 e-value: 5.0E-17 bit: 89.7 Len: 105 Query Start:176 Query End:280 Subject Strand: POSITIVE Subject Start: 145 Subject End: 249 ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaat................................ ...............................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcacggtaaaagcgtatgttttacgttgcaaaacagccgcttttggaatgcaaaacaataggttttataat................................ HSP 2 e-value: 3.0E-9 bit: 63.9 Len: 48 Query Start:196 Query End:243 Subject Strand: NEGATIVE Subject Start: 174 Subject End: 221 ...................................................................................................................................................................................................aaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaac..................................................................... ...................................................................................................................................................................................................aaagcggctgttttgcaacgtaaaacatacgcttttaccgtgcaaaac..................................................................... Subject: pint_PI1728_PI1729|ATP-dependent Clp protease, proteolytic subunit:FKBP-type peptidyl-prolyl cis-trans isomerase, trigger factor|POSITIVE:POSITIVE|[1647719,1648555]|837 HSP 1 e-value: 2.0E-16 bit: 87.7 Len: 128 Query Start:148 Query End:275 Subject Strand: NEGATIVE Subject Start: 416 Subject End: 543 ...................................................................................................................................................tgtaaaaataattcgtagagaaaatgacaatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt..................................... ...................................................................................................................................................tgtaaagataattcgttgagaaaacggtaatttcgatttgacgttgcaaaagcgtaggttttgcaaggcaaaagagccgcttttaccgtgcaaaagcggctcttttacaatgcgaaacaataggtttt..................................... Subject: pint_PI1271_PI1272|transcriptional regulator, AraC/XylS family:possible transcriptional regulator|POSITIVE:POSITIVE|[1191345,1191841]|497 HSP 1 e-value: 3.0E-6 bit: 54.0 Len: 103 Query Start:191 Query End:293 Subject Strand: POSITIVE Subject Start: 302 Subject End: 404 ..............................................................................................................................................................................................ttgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgtaatacgctggtaacga................... ..............................................................................................................................................................................................ttgcgaaagcggttcttttgcgatgcaaaacctacgcttttaccgtacaaaacagccgcttttggaacgcaaaagcgtaggttttgcaatacactgataacga................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCCGAAACAAGAAUAAACGGAGGUUUAAAAGACUUCCAACAAGAUAGAAUAAAAGAGGAUGUAUCAGAACUCUAAGUUUUGAUACACCCUCUUUUCUUUUGCAGUAAAAAAUCUUGUUAUUGCUUGAGAAGAAAGCCCUCGUUCUGUAAAAAUAAUUCGUAGAGAAAAUGACAAUUUCGAUUUGGCAUUGCGAAAGCGGCUGUUUUGCAUCGCAAAACAGCCGCUUUUACCGUGCAAAACCUACGCUUUUGGAAUGCAAAACAAUAGGUUUUGUAAUACGCUGGUAACGAGGUGGUUAUACAAUCGCUA ..(((.((.....((((....(((((((.....)))))))......(((((((..((((((.(((((((((((.....))))))))))).))))))(((((..(((((((.........)))))))..)))))..........))))))).......)))).........(((((.((((((..(..((.....(((((((((((((((((...)))))))))))))))))....(((((((((((...(((((.....)))))...)))))))))))....))..)...)))))).)))))....))))). (-108.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCGAUUGUAUAACCACCUCGUUACCAGCGUAUUACAAAACCUAUUGUUUUGCAUUCCAAAAGCGUAGGUUUUGCACGGUAAAAGCGGCUGUUUUGCGAUGCAAAACAGCCGCUUUCGCAAUGCCAAAUCGAAAUUGUCAUUUUCUCUACGAAUUAUUUUUACAGAACGAGGGCUUUCUUCUCAAGCAAUAACAAGAUUUUUUACUGCAAAAGAAAAGAGGGUGUAUCAAAACUUAGAGUUCUGAUACAUCCUCUUUUAUUCUAUCUUGUUGGAAGUCUUUUAAACCUCCGUUUAUUCUUGUUUCGGCUUA .................(((((((....((((....((((((((((.(((((........)))))))))))))))....(..((((((((((((((((...))))))))))))))))..).))))......((((.......))))....................)))))))...........((((...(((((((................((((((((((((((((.(((.....))).)))))))))))))))).............(((.((.......)).)))......)))))))...)))). (-97.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 907 270 221 121 72 pint:PI0851|5end_hypothetical_protein_784310..784540_POSITIVE 907 270 221 121 72 pint:PI0841|5end_hypothetical_protein_775406..775636_POSITIVE 881 270 221 77 28 pint:PI0755|5end_hypothetical_protein_686691..686921_POSITIVE 880 270 222 66 18 pint:PI0200|5end_hypothetical_protein_191545..191775_POSITIVE 880 270 222 66 18 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 879 270 221 231 182 pint:PI0159|5end_hypothetical_protein_151273..151503_POSITIVE 879 270 221 143 94 pint:PI0157|5end_hypothetical_protein_151185..151415_POSITIVE 819 230 181 180 131 pint:PI2111|5end_conserved_hypothetical_protein_2062771..2063001_POSITIVE 797 240 191 64 15 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 797 240 191 64 15 pint:PI1567|5end_hypothetical_protein_1489229..1489459_POSITIVE 797 240 191 206 157 pint:PI1486|5end_alginate_O-acetylation_protein_1405507..1405737_POSITIVE 797 240 191 190 141 pint:PI0788|5end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726152..726382_POSITIVE 770 270 222 66 18 pint:PI1733|5end_hypothetical_protein_1652160..1652390_POSITIVE 757 250 205 228 183 pint:PI0842|5end_5-formyltetrahydrofolate_cyclo-ligase_775533..775763_POSITIVE 739 240 191 64 15 pint:PI1934|5end_hypothetical_protein_1882363..1882593_POSITIVE 739 240 191 64 15 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 739 240 191 72 23 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 739 240 191 64 15 pint:PI1167|5end_hypothetical_protein_1089688..1089918_POSITIVE 739 240 191 113 64 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 725 240 191 195 146 pint:PI0771|5end_conserved_hypothetical_protein_706524..706754_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 875 240 191 146 97 pint:PI0158|3end_hypothetical_protein_151298..151448_POSITIVE 803 240 191 140 91 pint:PI0182|3end_hypothetical_protein_174277..174427_POSITIVE 771 220 171 147 98 pint:PI1905|3end_hypothetical_protein_1847854..1848004_POSITIVE 739 240 191 150 101 pint:PI1922|3end_hypothetical_protein_1865454..1865604_POSITIVE 739 240 191 146 97 pint:PI1340|3end_hypothetical_protein_1256872..1257022_POSITIVE 725 240 191 146 97 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 712 240 191 106 57 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 697 240 191 120 71 pint:PI1356|3end_hypothetical_protein_1273340..1273490_POSITIVE 697 279 231 58 10 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 693 279 231 58 10 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 693 279 231 52 4 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 693 279 231 86 38 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 692 240 191 146 97 pint:PI2011|3end_hypothetical_protein_1970730..1970880_POSITIVE 686 240 191 146 97 pint:PI0689|3end_hypothetical_protein_625877..626027_POSITIVE 681 280 231 146 97 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 667 240 191 146 97 pint:PI0061|3end_hypothetical_protein_60690..60840_POSITIVE 665 230 184 150 104 pint:PI1528|3end_hypothetical_protein_1449878..1450028_POSITIVE 663 279 231 54 6 pint:PI1051|3end_hypothetical_protein_970228..970378_POSITIVE 662 220 171 148 99 pint:PI1829|3end_hypothetical_protein_1769439..1769589_POSITIVE 658 240 191 146 97 pint:PI0004|3end_hypothetical_protein_6389..6539_POSITIVE