Origin IGS: taagatttctgcttgctgtggtccttcaataatacgttcttctttgtaaagacctgcgtctttaagtcctgcgagtgcatcacagagatgttgtttcattttaccatacatagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attattattgaatagatttttcgtttcgtgctgcaaagttaaatacttttttgattaatttaggaattagaagtattaatgttgtataaaaaaacagcgttactataaaataatctgcttttaagtttcttttctcgaaaaatatcaagacctttgtagttgaataattaaaaactttatgctatgtatggtaaaatgaaacaacatctctgtgatgcactcgcaggacttaaagacgcaggtctttacaaagaagaacgtattattgaaggaccacagcaagcagaaatctta Mask Tandem Repeat Region ================================================ taagatttctgcttgctgtggtccttcaataatacgttcttctttgtaaagacctgcgtctttaagtcctgcgagtgcatcacagagatgttgtttcattttaccatacatagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Find is-nt database================================================ Query_seq: PI0194:PI0195|PI0194:PI0195:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase):epimerase/reductase:->->:187018..187311 294 taagatttctgcttgctgtggtccttcaataatacgttcttctttgtaaagacctgcgtctttaagtcctgcgagtgcatcacagagatgttgtttcattttaccatacatagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0194:PI0195|PI0194:PI0195:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase):epimerase/reductase:->->:187018..187311 294 taagatttctgcttgctgtggtccttcaataatacgttcttctttgtaaagacctgcgtctttaagtcctgcgagtgcatcacagagatgttgtttcattttaccatacatagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0194:PI0195|PI0194:PI0195:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase):epimerase/reductase:->->:187018..187311 294 taagatttctgcttgctgtggtccttcaataatacgttcttctttgtaaagacctgcgtctttaagtcctgcgagtgcatcacagagatgttgtttcattttaccatacatagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 4 Max: 111 Len: 108 Subject: UniRef90_Q64RZ5 Cluster: 2-amino-3-ketobutyrate coenzyme A ligase; n=3; Bacteroides|Rep: 2-amino-3-ketobutyrate coenzyme A ligase - Bacteroides fragilis HSP 1 e-value: 2.0E-6 bit: 53.9 Len: 108 Query Start:4 Query End:111 Subject Strand: null Subject Start: 1 Subject End: 36 ... M Y G K M K Q H L C D A L A G L K D A G L Y K E E R I I E G P Q Q A E I ....................................................................................................................................................................................... ... M Y G K M K E H L S N T I A E I K E A G L Y K E E R L I E S A Q Q A A I ....................................................................................................................................................................................... taannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 13 End: 147 ............ M L W S F N N T F F F V K T C V F K S C E C I T E M L F H F T I H S I K F L I I Q L Q R S ................................................................................................................................................... Protein_Len: 47 Strand: + Start: 147 End: 287 .................................................................................................................................................. M I F F E K R N L K A D Y F I V T L F F Y T T L I L L I P K L I K K V F N F A A R N E K S I Q ....... Protein_Len: 37 Strand: - Start: 1 End: 111 L I E A Q Q P G E I I R E E K Y L G A D K L G A L A D C L H Q K M K G Y M ....................................................................................................................................................................................... taannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0194:PI0195|PI0194:PI0195:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase):epimerase/reductase:->->:187018..187311 294 taannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Intra-Species Hit: Count: 1 Min: 112 Max: 294 Len: 183 Subject: pint_PI0194_PI0195|2-amino-3-ketobutyrate coenzyme A ligase (glycine C- acetyltransferase):epimerase/reductase|POSITIVE:POSITIVE |[187018,187311]|294 HSP 1 e-value: 8.0E-87 bit: 321.0 Len: 183 Query Start:112 Query End:294 Subject Strand: POSITIVE Subject Start: 112 Subject End: 294 ...............................................................................................................agcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgnnnnnnnatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat ...............................................................................................................agcataaagtttttaattattcaactacaaaggtcttgatatttttcgagaaaagaaacttaaaagcagattattttatagtaacgctgtttttttatacaacattaatacttctaattcctaaattaatcaaaaaagtatttaactttgcagcacgaaacgaaaaatctattcaataataat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCAUAAAGUUUUUAAUUAUUCAACUACAAAGGUCUUGAUAUUUUUCGAGAAAAGAAACUUAAAAGCAGAUUAUUUUAUAGUAACGCUGUUUUUUUAUACAACAUUAAUACUUCUAAUUCCUAAAUUAAUCAAAAAAGUAUUUAACUUUGCAGCACGAAACGAAAAAUCUAUUCAAUAAUAAU ........((((((((...(((..((....)).((((((......))))))...)))..))))))))..((((((..((((......((((((......(((....(((((((......................))))))).....)))......))))))......))))..))))))... (-21.05) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAUUAUUGAAUAGAUUUUUCGUUUCGUGCUGCAAAGUUAAAUACUUUUUUGAUUAAUUUAGGAAUUAGAAGUAUUAAUGUUGUAUAAAAAAACAGCGUUACUAUAAAAUAAUCUGCUUUUAAGUUUCUUUUCUCGAAAAAUAUCAAGACCUUUGUAGUUGAAUAAUUAAAAACUUUAUGCU ...........((((((((((.(((....(((((((((........((((((((.......((((((((((((((.(((((((((........))))))))).............))))))).)))))))....)))))))).........))))))))).....))).))))).)))))... (-30.87) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 221 172 146 203 174 pint:PI0375|5end_hypothetical_protein_345634..345864_POSITIVE 211 70 28 210 167 pint:PI0370|5end_conserved_hypothetical_protein_340453..340683_POSITIVE 206 90 41 128 74 pint:PI0929|5end_copper_homeostasis_protein_860294..860524_POSITIVE 202 78 31 191 152 pint:PI0101|5end_lauroyl/myristoyl_acyltransferase_96729..96959_POSITIVE 201 41 2 131 89 pint:PI1591|5end_ISPg3-related_transposase_1518747..1518977_POSITIVE 201 89 67 58 35 pint:PI1025|5end_tRNA_modification_GTPase_940752..940982_POSITIVE 201 172 146 114 84 pint:PI0301|5end_probable_transposase_285672..285902_POSITIVE 200 92 59 182 145 pint:PI0988|5end_hypothetical_protein_912347..912577_POSITIVE 199 80 31 215 156 pint:PI1426|5end_flavodoxin_1344881..1345111_POSITIVE 197 88 43 174 124 pint:PI1871|5end_glycosyl_transferase,_group_1_family_protein_1815788..1816018_POSITIVE 194 177 134 217 168 pint:PI2109|5end_surface_antigen_BspA_2060553..2060783_POSITIVE 191 71 31 92 52 pint:PI0525|5end_transcriptional_regulator,_Arac_family_481332..481562_POSITIVE 190 157 135 67 47 pint:PI1380|5end_conserved_hypothetical_protein_1302587..1302817_POSITIVE 188 164 130 174 145 pint:PI0181|5end_conserved_hypothetical_protein_173435..173665_POSITIVE 187 70 27 125 81 pint:PI0786|5end_conserved_hypothetical_protein;_possible_glycosyltransferase_723240..723470_POSITIVE 183 160 146 162 148 pint:PI1753|5end_conserved_hypothetical_protein_1680291..1680521_POSITIVE 182 168 137 144 112 pint:PI0698|5end_conserved_hypothetical_protein_635372..635602_POSITIVE 181 87 54 154 124 pint:PI0148|5end_mannose-1-phosphate_guanylyltransferase_141423..141653_POSITIVE 180 161 147 45 31 pint:PI1076|5end_hypothetical_protein_998893..999123_POSITIVE 180 170 131 80 30 pint:PI0177|5end_2-oxoglutarate_synthase,_subunit_korB_168499..168729_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 227 70 21 99 57 pint:PI1543|3end_hypothetical_protein_1463281..1463431_POSITIVE 211 70 28 86 43 pint:PI0369|3end_hypothetical_protein_340409..340559_POSITIVE 202 78 31 108 69 pint:PI0100|3end_LPS_biosynthesis_protein_(lipopolysaccharide_biosynthesis_protein)_96726..96876_POSITIVE 201 172 146 123 93 pint:PI0300|3end_transposase_285761..285911_POSITIVE 200 92 59 121 84 pint:PI0986|3end_conserved_hypothetical_protein_912366..912516_POSITIVE 200 92 59 39 2 pint:PI0987|3end_hypothetical_protein_912284..912434_POSITIVE 199 80 31 103 44 pint:PI1425|3end_conserved_hypothetical_protein_1344849..1344999_POSITIVE 199 106 66 100 52 pint:PI0141|3end_glutamine_amidotransferase_involved_in_pyridoxine_biosynthesis_133308..133458_POSITIVE 190 110 61 49 11 pint:PI1021|3end_conserved_hypothetical_protein;_possible_methyltransferase_939634..939784_POSITIVE 188 164 130 89 60 pint:PI0180|3end_TonB_protein_173430..173580_POSITIVE 187 70 27 108 64 pint:PI0784|3end_possible_amidase_enhancer_precursor_723303..723453_POSITIVE 183 160 146 71 57 pint:PI1751|3end_desulfoferrodoxin_1680280..1680430_POSITIVE 182 96 52 68 19 pint:PI0145|3end_secDF_export_membrane_protein_139724..139874_POSITIVE 180 161 147 137 123 pint:PI1076|3end_hypothetical_protein_999065..999215_POSITIVE 178 163 132 57 27 pint:PI1271|3end_transcriptional_regulator,__AraC/XylS_family_1191284..1191434_POSITIVE 177 175 132 56 10 pint:PI1306|3end_dipeptidyl_peptidase_IV_1222452..1222602_POSITIVE 177 46 26 122 99 pint:PI0124|3end_outer_membrane_protein/protective_antigen_OMA87_117182..117332_POSITIVE 175 46 4 123 73 pint:PI0442|3end_50S_ribosomal_protein_L1_412413..412563_POSITIVE 172 89 63 64 27 pint:PI0912|3end_conserved_hypothetical_protein_842692..842842_POSITIVE 171 88 47 111 75 pint:PI0034|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_37833..37983_POSITIVE