Origin IGS: atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatcgcagacactatcagtataatataataaggtggatttaagacaacgttggttgtgttaaacccaccttttttgttttgcgcatctacactttttcttctgtctacttacagattagcagaactttattgaagagaatgtctcatacaagcaaaatgtcaactatttaaatatcgaacaaatat Mask Tandem Repeat Region ================================================ atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Find is-nt database================================================ Query_seq: PI0193:PI0194|PI0193:PI0194:conserved hypothetical protein:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase):->->:185756..185943 188 atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0193:PI0194|PI0193:PI0194:conserved hypothetical protein:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase):->->:185756..185943 188 atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0193:PI0194|PI0193:PI0194:conserved hypothetical protein:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase):->->:185756..185943 188 atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 19 End: 108 .................. I T S M K S T H S M R K L L T R S I Q L Y V S S F T Y I R M ................................................................................ atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -15.1 Tail_Score: -4.01089 Start: 118 End: 155 Full_Region: GCGCAAAACAAAAAA GGTGGGTTTAACACAAC CAAC GTTGTCTTAAATCCACC TTATTATATTATACT .....................................................................................................................GGTGGGTTTAACACAACCAACGTTGTCTTAAATCCACC................................. TransTerm Strand: - Conf: 100 HP_score: -12.9 Tail_Score: -5.79595 Start: 118 End: 155 Full_Region: agtataatataataa ggtggatttaagacaac gttg gttgtgttaaacccacc ttttttgttttgcgc .....................................................................................................................ggtggatttaagacaacgttggttgtgttaaacccacc................................. Find igs database================================================ Query_seq: PI0193:PI0194|PI0193:PI0194:conserved hypothetical protein:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase):->->:185756..185943 188 atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Intra-Species Hit: Count: 1 Min: 1 Max: 188 Len: 188 Subject: pint_PI0193_PI0194|conserved hypothetical protein:2-amino-3-ketobutyrate coenzyme A ligase (glycine C-acetyltransferase)|POSITIVE:POSITIVE|[185756, 185943]|188 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 188 Query Start:1 Query End:188 Subject Strand: POSITIVE Subject Start: 1 Subject End: 188 atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta atatttgttcgatatttaaatagttgacattttgcttgtatgagacattctcttcaataaagttctgctaatctgtaagtagacagaagaaaaagtgtagatgcgcaaaacaaaaaaggtgggtttaacacaaccaacgttgtcttaaatccaccttattatattatactgatagtgtctgcgattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUUUGUUCGAUAUUUAAAUAGUUGACAUUUUGCUUGUAUGAGACAUUCUCUUCAAUAAAGUUCUGCUAAUCUGUAAGUAGACAGAAGAAAAAGUGUAGAUGCGCAAAACAAAAAAGGUGGGUUUAACACAACCAACGUUGUCUUAAAUCCACCUUAUUAUAUUAUACUGAUAGUGUCUGCGAUUUA ((((((.(((..........((((.((.((((((.(((...(((.....)))..))))))))))).)))).(((((......))))).))).)))))).....((((........(((((((((((((.(((((....))))).)))))))))))))...(((((((....))))))).))))..... (-47.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUCGCAGACACUAUCAGUAUAAUAUAAUAAGGUGGAUUUAAGACAACGUUGGUUGUGUUAAACCCACCUUUUUUGUUUUGCGCAUCUACACUUUUUCUUCUGUCUACUUACAGAUUAGCAGAACUUUAUUGAAGAGAAUGUCUCAUACAAGCAAAAUGUCAACUAUUUAAAUAUCGAACAAAUAU (((((.(..((((...((((((.........(((((((.(((((.(((((....))))).))))).)))))))....(((((((.................(((((......)))))...)))))))..)))))).(((.....)))............))))..).)))))................ (-41.85) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 267 70 22 90 47 pint:PI1222|5end_hypothetical_protein_1142834..1143064_POSITIVE 256 83 47 67 31 pint:PI0195|5end_epimerase/reductase_187172..187402_POSITIVE 253 107 65 86 35 pint:PI0063|5end_transcriptional_regulator,_GntR_family_63181..63411_POSITIVE 247 124 81 156 109 pint:PI0060|5end_long-chain-fatty-acid--CoA_ligase_(acyl-CoA_synthetase)_58281..58511_POSITIVE 242 107 66 88 49 pint:PI0275|5end_hypothetical_protein_263138..263368_POSITIVE 241 108 61 164 125 pint:PI0980|5end_conserved_hypothetical_protein_905703..905933_POSITIVE 241 157 116 160 124 pint:PI0033|5end_peptidylprolyl_isomerase_36215..36445_POSITIVE 237 107 61 66 13 pint:PI1789|5end_hypothetical_protein_1718727..1718957_POSITIVE 237 119 72 135 84 pint:PI1460|5end_ribonucleotide_reductase,_alpha_subunit,_N-terminal_region_1382215..1382445_POSITIVE 236 69 33 50 16 pint:PI0812|5end_conserved_hypothetical_protein;_possible_ferredoxin_750185..750415_POSITIVE 234 149 118 210 178 pint:PI0816|5end_glycogen_debranching_enzyme_754930..755160_POSITIVE 232 125 81 161 117 pint:PI0644|5end_dnaJ_protein_585704..585934_POSITIVE 232 104 62 112 51 pint:PI0584|5end_hypothetical_protein_527352..527582_POSITIVE 232 127 84 199 146 pint:PI0151|5end_ISPg4-related_transposase_(IS5_family)_143805..144035_POSITIVE 231 137 91 225 174 pint:PI1949|5end_aminotransferase,_class_V_1903218..1903448_POSITIVE 231 137 91 91 40 pint:PI1948|5end_hypothetical_protein_1903084..1903314_POSITIVE 231 154 111 56 16 pint:PI1850|5end_prenyltransferase,_UbiA_family_1795967..1796197_POSITIVE 227 146 118 63 26 pint:PI1641|5end_hypothetical_protein_1558495..1558725_POSITIVE 226 144 105 154 114 pint:PI0042|5end_hypothetical_protein_42981..43211_POSITIVE 226 144 105 138 98 pint:PI0044|5end_thiamine_biosynthetic_enzyme_42965..43195_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 107 66 57 18 pint:PI0274|3end_conserved_hypothetical_protein_263187..263337_POSITIVE 241 77 48 54 25 pint:PI1915|3end_conserved_hypothetical_protein_1859906..1860056_POSITIVE 241 108 61 59 20 pint:PI0979|3end_transcriptional_regulator,_tetR_family_905678..905828_POSITIVE 241 157 116 119 83 pint:PI0032|3end_hypothetical_protein_36254..36404_POSITIVE 238 105 66 151 115 pint:PI0273|3end_hypothetical_protein_263284..263434_POSITIVE 237 119 72 148 97 pint:PI1459|3end_ribonucleotide_reductase,_alpha_subunit,_C-terminal_region_1382308..1382458_POSITIVE 236 109 63 142 112 pint:PI1738|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_1660112..1660262_POSITIVE 233 143 102 115 70 pint:PI1844|3end_hypothetical_protein_1790279..1790429_POSITIVE 232 127 84 147 94 pint:PI0150|3end_hypothetical_protein_143833..143983_POSITIVE 232 110 61 110 72 pint:PI0062|3end_endopeptidase_63022..63172_POSITIVE 231 154 111 48 8 pint:PI1849|3end_conserved_hypothetical_protein;_possible_acyltransferase_family_protein_1796039..1796189_POSITIVE 227 146 118 46 9 pint:PI1640|3end_hypothetical_protein_1558558..1558708_POSITIVE 223 107 62 83 40 pint:PI1487|3end_hypothetical_protein_1407940..1408090_POSITIVE 218 106 61 147 105 pint:PI1008|3end_cytochrome_c_nitrite_reductase,_small_subunit_929331..929481_POSITIVE 217 124 85 77 41 pint:PI0006|3end_hypothetical_protein_8268..8418_POSITIVE 215 142 102 45 12 pint:PI1813|3end_hypothetical_protein_1753086..1753236_POSITIVE 215 105 61 58 22 pint:PI1221|3end_penicillin-binding_protein_1A_1142855..1143005_POSITIVE 214 108 62 67 15 pint:PI0226|3end_ABC_transporter,_ATP-binding_/_permease_protein_223884..224034_POSITIVE 212 103 78 47 25 pint:PI1540|3end_hypothetical_protein_1461771..1461921_POSITIVE 212 156 129 52 27 pint:PI0386|3end_integrase_354640..354790_POSITIVE