Origin IGS: tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagataaaacattaataagagagtaatcgaagctttgagtttcgattactctcttttcttttgcagcaaaaaatctttctgttgcccagcaaaaacgctcttattctgtaaagataattcgtaggaaaaatgataatttcgatttggca Mask Tandem Repeat Region ================================================ tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Find is-nt database================================================ Query_seq: PI0182:PI0183|PI0182:PI0183:hypothetical protein:hypothetical protein:->->:174338..174486 149 tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0182:PI0183|PI0182:PI0183:hypothetical protein:hypothetical protein:->->:174338..174486 149 tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0182:PI0183|PI0182:PI0183:hypothetical protein:hypothetical protein:->->:174338..174486 149 tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 7 End: 135 ...... M E I I I F P T N Y L Y R I R A F L L G N R K I F C C K R K E S N R N S K L R L L S Y .............. Protein_Len: 37 Strand: - Start: 29 End: 139 ............................ S N D K C F L L T K A P C C F S K K S C F F L S Y D F S L A E I V R K N M .......... Protein_Len: 35 Strand: - Start: 1 End: 105 A L D F N D N K R R I I K V S Y S R K Q Q A V S L N K A A F S F L T M ............................................ tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -17.4 Tail_Score: -3.90315 Start: 97 End: 131 Full_Region: TGCTGCAAAAGAAAA GAGAGTAATCGAAACT CAA AGCTTCGATTACTCTC TTATTAATGTTTTAT ................................................................................................GAGAGTAATCGAAACTCAAAGCTTCGATTACTCTC.................. Find igs database================================================ Query_seq: PI0182:PI0183|PI0182:PI0183:hypothetical protein:hypothetical protein:->->:174338..174486 149 tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Intra-Species Hit: Count: 1 Min: 1 Max: 149 Len: 149 Subject: pint_PI0182_PI0183|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[174338,174486]|149 HSP 1 e-value: 2.0E-79 bit: 295.0 Len: 149 Query Start:1 Query End:149 Subject Strand: POSITIVE Subject Start: 1 Subject End: 149 tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta tgccaaatcgaaattatcatttttcctacgaattatctttacagaataagagcgtttttgctgggcaacagaaagattttttgctgcaaaagaaaagagagtaatcgaaactcaaagcttcgattactctcttattaatgttttatcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCCAAAUCGAAAUUAUCAUUUUUCCUACGAAUUAUCUUUACAGAAUAAGAGCGUUUUUGCUGGGCAACAGAAAGAUUUUUUGCUGCAAAAGAAAAGAGAGUAAUCGAAACUCAAAGCUUCGAUUACUCUCUUAUUAAUGUUUUAUCUA (((......((((........))))...((((..((((((..........((((....)))).(....)..))))))..))))..)))......(((((((((((((((.(.....).)))))))))))))))................ (-35.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAUAAAACAUUAAUAAGAGAGUAAUCGAAGCUUUGAGUUUCGAUUACUCUCUUUUCUUUUGCAGCAAAAAAUCUUUCUGUUGCCCAGCAAAAACGCUCUUAUUCUGUAAAGAUAAUUCGUAGGAAAAAUGAUAAUUUCGAUUUGGCA ................(((((((((((((((((.....)))))))))))))))))...((((((.((((............))))...))))))..(((....(((((..((.....))..)))))....(((.....)))....))). (-36.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 530 50 1 135 86 pint:PI0037|5end_hypothetical_protein_39354..39584_POSITIVE 509 49 1 94 46 pint:PI0815|5end_hypothetical_protein_754475..754705_POSITIVE 492 47 1 132 86 pint:PI0322|5end_hypothetical_protein_303508..303738_POSITIVE 435 49 1 75 26 pint:PI1182|5end_hypothetical_protein_1104025..1104255_POSITIVE 431 50 1 115 64 pint:PI0638|5end_hypothetical_protein_582218..582448_POSITIVE 407 46 1 80 35 pint:PI2141|5end_hypothetical_protein_2087169..2087399_POSITIVE 399 133 95 154 116 pint:PI0184|5end_possible_fibronectin_type_III_domain_protein_174355..174585_POSITIVE 385 45 1 130 86 pint:PI1436|5end_hypothetical_protein_1355961..1356191_POSITIVE 366 46 1 223 178 pint:PI1804|5end_hypothetical_protein_1738109..1738339_POSITIVE 358 49 1 76 28 pint:PI1041|5end_hypothetical_protein_960443..960673_POSITIVE 357 60 11 188 136 pint:PI0119|5end_hypothetical_protein_111506..111736_POSITIVE 345 50 1 63 13 pint:PI1540|5end_hypothetical_protein_1461362..1461592_POSITIVE 340 47 1 111 65 pint:PI1786|5end_hypothetical_protein_1718124..1718354_POSITIVE 331 48 1 60 13 pint:PI0970|5end_hypothetical_protein_897254..897484_POSITIVE 323 71 32 229 180 pint:PI1542|5end_mobilization_protein_1461728..1461958_POSITIVE 323 71 32 105 56 pint:PI1541|5end_hypothetical_protein_1461604..1461834_POSITIVE 315 48 1 119 72 pint:PI0659|5end_hypothetical_protein_603203..603433_POSITIVE 311 43 4 128 89 pint:PI0926|5end_hypothetical_protein_856824..857054_POSITIVE 307 49 1 82 34 pint:PI1064|5end_hypothetical_protein_983071..983301_POSITIVE 299 45 1 193 148 pint:PI1371|5end_hypothetical_protein_1292359..1292589_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 436 48 4 83 39 pint:PI0574|3end_hypothetical_protein_519444..519594_POSITIVE 387 46 1 95 50 pint:PI1520|3end_hypothetical_protein_1441370..1441520_POSITIVE 289 72 51 33 11 pint:PI0526|3end_hypothetical_protein_482439..482589_POSITIVE 277 76 33 60 14 pint:PI1063|3end_excinuclease_ABC,_subunit_B_983099..983249_POSITIVE 274 64 30 47 6 pint:PI1577|3end_conserved_hypothetical_protein_1503879..1504029_POSITIVE 267 79 49 151 127 pint:PI2041|3end_conserved_hypothetical_protein_1994660..1994810_POSITIVE 265 69 25 68 33 pint:PI1380|3end_conserved_hypothetical_protein_1303242..1303392_POSITIVE 263 94 52 89 47 pint:PI0071|3end_hypothetical_protein_70229..70379_POSITIVE 258 86 50 96 53 pint:PI0056|3end_conserved_hypothetical_protein;_possible_DNA_uptake_protein_55907..56057_POSITIVE 255 130 82 51 1 pint:PI0647|3end_potassium_uptake_protein_589276..589426_POSITIVE 249 54 17 39 3 pint:PI1379|3end_hypothetical_protein_1302292..1302442_POSITIVE 247 87 52 47 19 pint:PI0891|3end_transketolase_822905..823055_POSITIVE 245 77 34 89 41 pint:PI1388|3end_transcriptional_regulator,_LuxR_family_1310024..1310174_POSITIVE 240 89 43 105 49 pint:PI1722|3end_possible_peptidyl-prolyl_cis-trans_isomerase_1640995..1641145_POSITIVE 239 100 58 80 21 pint:PI1140|3end_ABC_transporter,_ATP-binding_protein_1069417..1069567_POSITIVE 238 70 36 36 9 pint:PI1261|3end_ISPg3-related_transposase,_N-terminal_region_1184074..1184224_POSITIVE 238 89 41 132 69 pint:PI0660|3end_conserved_hypothetical_protein_603997..604147_POSITIVE 236 72 50 145 123 pint:PI0285|3end_succinyltransferase_protein_271292..271442_POSITIVE 236 75 36 79 39 pint:PI0194|3end_2-amino-3-ketobutyrate_coenzyme_A_ligase_(glycine_C-acetyltransferase)_186957..187107_POSITIVE 234 87 50 56 21 pint:PI1366|3end_conserved_hypothetical_protein;_possible_hydrolase,_HAD_superfamily_1286647..1286797_POSITIVE