Origin IGS: aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aattaaaattatatatttgattttttgagtttacaactctttatttaaagaataagcaattatcgaagctgccttatatctgtccttgcaactaaactaacttatacgaaaaattaaaaagaaaagttcatcaagacctcttttcttgtcgaaggtggcgaagtgtttctcgcacacgcacgtatattaaaatatttaaataaggatttaatcacaatgtatatcaagtatatcaacgaaatatctgctctaaccaaatacaaaatgttatagattgcagggaaaactctatttccttgcaacccatctatatctttattatttagtgggaaaagatacattcctaagttatttaacttcagaggcatacaacctatatatttacaaatggggtacaaaggtacttaaattatctgtacttacaaaataatattaaaaagtattgcacactttctgcgaaaacgattgctcaccgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattgcttacgtgtagaaaaatatttctttacgtgtaagtaattctttaattttacgctttcgagggagagacaaggcgttatcaaaagaaccttcgctacttattaatagtgggggttacttctttaaacaatacaaatttaactgtttaaagacacgttcagagatattttagcagatttaaactgatatcaaatagctatactatgcaaagaataataaacattttgtcatttaaatttggttcttaatgacgtttatcatatctttgcatatatcagaatttatctttaaaacatcacattatgagaaaagcctatttattcacattacttttacct Mask Tandem Repeat Region ================================================ aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt Find is-nt database================================================ Query_seq: PI0136:PI0137|PI0136:PI0137:surface antigen BspA:lectin-like adhesin precursor:->->:128145..129004 860 aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0136:PI0137|PI0136:PI0137:surface antigen BspA:lectin-like adhesin precursor:->->:128145..129004 860 aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0136:PI0137|PI0136:PI0137:surface antigen BspA:lectin-like adhesin precursor:->->:128145..129004 860 aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 237 End: 332 ............................................................................................................................................................................................................................................ M S S E G S F D N A L S L P R K R K I K E L L T R K E I F F Y T ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 463 End: 744 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M Y P I C K Y I G C M P L K L N N L G M Y L F P L N N K D I D G L Q G N R V F P A I Y N I L Y L V R A D I S L I Y L I Y .................................................................................................................... Protein_Len: 47 Strand: - Start: 591 End: 731 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. L M K Y K T L A S I E N I Y K I Y M T I L D K N L Y K L I R A H A L F V E G G E V L F S T K M ................................................................................................................................. Protein_Len: 44 Strand: - Start: 456 End: 587 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................... T G K Y G M Q L Y I P Q I G R F N F L K P I Y R K G S F L L S I S P N C P F L T K G A M ................................................................................................................................................................................................................................................................................. Protein_Len: 51 Strand: - Start: 434 End: 586 ................................................................................................................................................................................................................................................................................................................................................................................................................................................. K T L V S L K L V K T G W K Y I Y L N Y A E S T L Y S L F T D K G V L Y Y L Y L H T A L F Y L K G Q M .................................................................................................................................................................................................................................................................................. Protein_Len: 37 Strand: - Start: 373 End: 483 .................................................................................................................................................................................................................................................................................................................................................................................... T Y T I R H A I T K A S L T C Y K K I N N Q L Y L Y N L Y R Q V G N T F M ......................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 329 End: 418 ........................................................................................................................................................................................................................................................................................................................................ V Y A I I E M C T F L Y K K V H L Y N P S C D N E C F T H M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: - Start: 303 End: 452 .............................................................................................................................................................................................................................................................................................................. K C T F F Y K E V R L C Y N G Y V Y I F I E K R T L L E T L L R K R L F H A I S K L I I K Y T C I M ........................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 50 Strand: - Start: 170 End: 319 ......................................................................................................................................................................... F I E S R T K L C N F K Y Q K F F Y G G S N I L L S P E K S L A K D R G R F R L I L S N S V R L S M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 34 Strand: - Start: 2 End: 103 . T F T I H I F L S K E Y H S T K F I F E S I H L S I I F T M L F W M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 85 Start: 824 End: 853 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTTGTAAACTCAAAAAATCAAATATATAAT....... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 45 HP_score: -2.9 Tail_Score: -3.29892 Start: 553 End: 592 Full_Region: ccaaatacaaaatgt tatagattgcagggaaa actcta tttccttgcaacccatc tatatctttattatt ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatagattgcagggaaaactctatttccttgcaacccatc............................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 57 HP_score: -5.5 Tail_Score: -3.19051 Start: 555 End: 590 Full_Region: aaatacaaaatgtta tagattgcagggaaa actcta tttccttgcaaccca tctatatctttatta ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tagattgcagggaaaactctatttccttgcaaccca.............................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 64 HP_score: -7.1 Tail_Score: -2.95339 Start: 559 End: 586 Full_Region: AAAGATATAGATGGG TTGCAAGGAAA TAGAGT TTTCCCTGCAA TCTATAACATTTTGT ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTGCAAGGAAATAGAGTTTTCCCTGCAA.................................................................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: PI0136:PI0137|PI0136:PI0137:surface antigen BspA:lectin-like adhesin precursor:->->:128145..129004 860 aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt Intra-Species Hit: Count: 1 Min: 1 Max: 860 Len: 860 Subject: pint_PI0136_PI0137|surface antigen BspA:lectin-like adhesin precursor|POSITIVE:POSITIVE|[128145,129004]|860 HSP 1 e-value: 0.0 bit: 1705.0 Len: 860 Query Start:1 Query End:860 Subject Strand: POSITIVE Subject Start: 1 Subject End: 860 aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt aggtaaaagtaatgtgaataaataggcttttctcataatgtgatgttttaaagataaattctgatatatgcaaagatatgataaacgtcattaagaaccaaatttaaatgacaaaatgtttattattctttgcatagtatagctatttgatatcagtttaaatctgctaaaatatctctgaacgtgtctttaaacagttaaatttgtattgtttaaagaagtaacccccactattaataagtagcgaaggttcttttgataacgccttgtctctccctcgaaagcgtaaaattaaagaattacttacacgtaaagaaatatttttctacacgtaagcaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcggtgagcaatcgttttcgcagaaagtgtgcaatactttttaatattattttgtaagtacagataatttaagtacctttgtaccccatttgtaaatatataggttgtatgcctctgaagttaaataacttaggaatgtatcttttcccactaaataataaagatatagatgggttgcaaggaaatagagttttccctgcaatctataacattttgtatttggttagagcagatatttcgttgatatacttgatatacattgtgattaaatccttatttaaatattttaatatacgtgcgtgtgcgagaaacacttcgccaccttcgacaagaaaagaggtcttgatgaacttttctttttaatttttcgtataagttagtttagttgcaaggacagatataaggcagcttcgataattgcttattctttaaataaagagttgtaaactcaaaaaatcaaatatataattttaatt HSP 2 e-value: 8.0E-6 bit: 54.0 Len: 27 Query Start:360 Query End:386 Subject Strand: NEGATIVE Subject Start: 297 Subject End: 323 .......................................................................................................................................................................................................................................................................................................................................................................aaatatttctttacgtgtaagtaattc.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .......................................................................................................................................................................................................................................................................................................................................................................aaatatttctttacgtgtaagtaattc.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-6 bit: 54.0 Len: 27 Query Start:297 Query End:323 Subject Strand: NEGATIVE Subject Start: 360 Subject End: 386 ........................................................................................................................................................................................................................................................................................................gaattacttacacgtaaagaaatattt......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ........................................................................................................................................................................................................................................................................................................gaattacttacacgtaaagaaatattt......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGUAAAAGUAAUGUGAAUAAAUAGGCUUUUCUCAUAAUGUGAUGUUUUAAAGAUAAAUUCUGAUAUAUGCAAAGAUAUGAUAAACGUCAUUAAGAACCAAAUUUAAAUGACAAAAUGUUUAUUAUUCUUUGCAUAGUAUAGCUAUUUGAUAUCAGUUUAAAUCUGCUAAAAUAUCUCUGAACGUGUCUUUAAACAGUUAAAUUUGUAUUGUUUAAAGAAGUAACCCCCACUAUUAAUAAGUAGCGAAGGUUCUUUUGAUAACGCCUUGUCUCUCCCUCGAAAGCGUAAAAUUAAAGAAUUACUUACACGUAAAGAAAUAUUUUUCUACACGUAAGCAAUAAUUUCCAUACACGUAAAUAAAUAUUUCUUUACGUGUAAGUAAUUCGGUGAGCAAUCGUUUUCGCAGAAAGUGUGCAAUACUUUUUAAUAUUAUUUUGUAAGUACAGAUAAUUUAAGUACCUUUGUACCCCAUUUGUAAAUAUAUAGGUUGUAUGCCUCUGAAGUUAAAUAACUUAGGAAUGUAUCUUUUCCCACUAAAUAAUAAAGAUAUAGAUGGGUUGCAAGGAAAUAGAGUUUUCCCUGCAAUCUAUAACAUUUUGUAUUUGGUUAGAGCAGAUAUUUCGUUGAUAUACUUGAUAUACAUUGUGAUUAAAUCCUUAUUUAAAUAUUUUAAUAUACGUGCGUGUGCGAGAAACACUUCGCCACCUUCGACAAGAAAAGAGGUCUUGAUGAACUUUUCUUUUUAAUUUUUCGUAUAAGUUAGUUUAGUUGCAAGGACAGAUAUAAGGCAGCUUCGAUAAUUGCUUAUUCUUUAAAUAAAGAGUUGUAAACUCAAAAAAUCAAAUAUAUAAUUUUAAUU .(..((((((..(((......))).))))))..)............((((((((((..(((.((((((((((((((.(((((((((((((((.(((......))).)))))).....)))))))))))))))))))...((((.((((((.......))))))..))))...))))...)))..)))))))))).(((((((.(((((((((((((((((((((.......((((((.(((.(((....))))))))))))..(((((((.(((..((((((((((.....(((..(((((((((((((((((((((((((((...(((..(((.(.((....)).).)))..)))...)))))))))))))))))))))))))))..))).....)))))))).)).(((((((((((........((((.((((((((..(((((((.......((((....))))...)))))))..)))).)))).))))...(((.(((((....))))).)))..((((((.(((..((((((((...(((((....((((((((((.(((((......))))).))))))))))...))))).))))))))..))).))))))...)))).)))))))........(((..(((((((.(((((.................((...(((.(((((......))))))))...))..((((((((...((.....)).)))))))).............)))))...)))))))..)))....))).)))))))...........)))))...))))))))))..((((.....))))............)))))).))))))) (-215.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUAAAAUUAUAUAUUUGAUUUUUUGAGUUUACAACUCUUUAUUUAAAGAAUAAGCAAUUAUCGAAGCUGCCUUAUAUCUGUCCUUGCAACUAAACUAACUUAUACGAAAAAUUAAAAAGAAAAGUUCAUCAAGACCUCUUUUCUUGUCGAAGGUGGCGAAGUGUUUCUCGCACACGCACGUAUAUUAAAAUAUUUAAAUAAGGAUUUAAUCACAAUGUAUAUCAAGUAUAUCAACGAAAUAUCUGCUCUAACCAAAUACAAAAUGUUAUAGAUUGCAGGGAAAACUCUAUUUCCUUGCAACCCAUCUAUAUCUUUAUUAUUUAGUGGGAAAAGAUACAUUCCUAAGUUAUUUAACUUCAGAGGCAUACAACCUAUAUAUUUACAAAUGGGGUACAAAGGUACUUAAAUUAUCUGUACUUACAAAAUAAUAUUAAAAAGUAUUGCACACUUUCUGCGAAAACGAUUGCUCACCGAAUUACUUACACGUAAAGAAAUAUUUAUUUACGUGUAUGGAAAUUAUUGCUUACGUGUAGAAAAAUAUUUCUUUACGUGUAAGUAAUUCUUUAAUUUUACGCUUUCGAGGGAGAGACAAGGCGUUAUCAAAAGAACCUUCGCUACUUAUUAAUAGUGGGGGUUACUUCUUUAAACAAUACAAAUUUAACUGUUUAAAGACACGUUCAGAGAUAUUUUAGCAGAUUUAAACUGAUAUCAAAUAGCUAUACUAUGCAAAGAAUAAUAAACAUUUUGUCAUUUAAAUUUGGUUCUUAAUGACGUUUAUCAUAUCUUUGCAUAUAUCAGAAUUUAUCUUUAAAACAUCACAUUAUGAGAAAAGCCUAUUUAUUCACAUUACUUUUACCU .......(((((...((((((..((...(((((....(((((....))))).)))))))..))))))..((((((((....(((((((..............((((((..........((((((((.((.....))...)))))))).......(((((((........)))).)))...))))))..............)))))))........((((((((....((((..(.......(((((..(((.((.(((((..(((.(.(((((((((((((((((......))))))))))....))))))).))))..))))).)).)))..))))).....(((((((....))))).)).)..))))....)))))))).....))))))))...((((((...........)))))).....)))))....(((((((.((....((((((.(((((.((..........(((((((((((((((((((((((((((.((((((((((.(.((....)).).)))))))))).)))))))))))))))))))))))))))..........)).)))))..))))))...((((....(((....(((((((((((........)))))))))))...((((((((((.............))))))))))..........(((.((((((.((((.....((((((..............((((((((((((.((((((.....((((((..............)))))))))))).)).)))))))))))))))).....)))))))))).)))......))).....)))).........)).))))))).... (-201.53) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 653 387 341 168 122 pint:PI0130|5end_hypothetical_protein_122032..122262_POSITIVE 426 369 321 195 147 pint:PI0115|5end_hypothetical_protein_108062..108292_POSITIVE 426 369 321 181 133 pint:PI0111|5end_hypothetical_protein_108048..108278_POSITIVE 426 369 321 146 98 pint:PI0112|5end_hypothetical_protein_108013..108243_POSITIVE 404 334 293 130 85 pint:PI0983|5end_hypothetical_protein_909750..909980_POSITIVE 399 335 297 94 56 pint:PI1450|5end_hypothetical_protein_1372683..1372913_POSITIVE 385 383 344 132 93 pint:PI0619|5end_hypothetical_protein_569364..569594_POSITIVE 349 390 342 146 97 pint:PI0312|5end_hypothetical_protein_294591..294821_POSITIVE 346 336 296 139 95 pint:PI1462|5end_hypothetical_protein_1385094..1385324_POSITIVE 319 724 683 89 38 pint:PI1501|5end_GTP-binding_elongation_factor_family_protein_TypA/BipA_1421424..1421654_POSITIVE 313 398 352 225 182 pint:PI0173|5end_hypothetical_protein_165305..165535_POSITIVE 299 805 764 51 6 pint:PI0460|5end_50S_ribosomal_protein_L3_432471..432701_POSITIVE 293 289 241 77 15 pint:PI0351|5end_hypothetical_protein_324317..324547_POSITIVE 291 405 362 66 24 pint:PI1256|5end_conserved_hypothetical_protein_1177481..1177711_POSITIVE 291 724 682 202 158 pint:PI0060|5end_long-chain-fatty-acid--CoA_ligase_(acyl-CoA_synthetase)_58281..58511_POSITIVE 290 394 351 178 132 pint:PI1673|5end_hypothetical_protein_1587718..1587948_POSITIVE 286 724 681 66 10 pint:PI1895|5end_penicillin-binding_protein_2_1838460..1838690_POSITIVE 286 729 686 41 1 pint:PI0543|5end_adenylate_kinase_495073..495303_POSITIVE 283 284 243 198 165 pint:PI2115|5end_hypothetical_protein_2064966..2065196_POSITIVE 281 575 532 129 85 pint:PI0945|5end_sodium:solute_symporter_family_protein_876249..876479_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 687 390 341 62 12 pint:PI0598|3end_hypothetical_protein_547233..547383_POSITIVE 372 399 351 71 12 pint:PI0808|3end_ISPg3-related_transposase_746457..746607_POSITIVE 345 336 299 38 1 pint:PI1461|3end_conserved_hypothetical_protein_1385073..1385223_POSITIVE 313 398 352 78 35 pint:PI0172|3end_ISPg3-related_transposase_165238..165388_POSITIVE 298 711 679 151 117 pint:PI2019|3end_elongation_factor_P_1976774..1976924_POSITIVE 294 395 351 145 95 pint:PI0725|3end_hypothetical_protein_658933..659083_POSITIVE 280 416 375 78 36 pint:PI1733|3end_hypothetical_protein_1652437..1652587_POSITIVE 280 345 301 75 34 pint:PI0701|3end_hypothetical_protein_638537..638687_POSITIVE 279 380 349 51 20 pint:PI1314|3end_hypothetical_protein_1231423..1231573_POSITIVE 277 410 369 39 4 pint:PI0491|3end_hypothetical_protein_451227..451377_POSITIVE 276 620 578 74 34 pint:PI1610|3end_Na+/H+_anti-porter_1538002..1538152_POSITIVE 274 386 346 145 112 pint:PI2082|3end_hypothetical_protein_2040263..2040413_POSITIVE 274 723 681 54 17 pint:PI1815|3end_polyphosphate-selective_porin_O_1754510..1754660_POSITIVE 272 417 371 74 30 pint:PI0157|3end_hypothetical_protein_151387..151537_POSITIVE 270 805 772 113 84 pint:PI0202|3end_DNA_helicase_195189..195339_POSITIVE 269 288 244 126 78 pint:PI1882|3end_hypothetical_protein_1826487..1826637_POSITIVE 269 498 464 135 95 pint:PI1462|3end_hypothetical_protein_1385383..1385533_POSITIVE 266 416 374 62 12 pint:PI2122|3end_conserved_hypothetical_protein_2070480..2070630_POSITIVE 265 280 239 118 81 pint:PI1184|3end_conserved_hypothetical_protein_1106163..1106313_POSITIVE 264 729 697 145 116 pint:PI0838|3end_DNA_replication_and_repair_protein_773927..774077_POSITIVE