Origin IGS: ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaagaaatgtttttctacacgtaagtaataatttccatacacgtaaataaatatttctttacgtgtaagtaattcgacagacaaaacgtttcggaacacataccccactgtaaaatagaagaaaaggggcaatattatgctataaaacaaaaccttccgtttaagaggg Mask Tandem Repeat Region ================================================ ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Find is-nt database================================================ Query_seq: PI0129:PI0130|PI0129:PI0130:hypothetical protein:hypothetical protein:->->:122002..122171 170 ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0129:PI0130|PI0129:PI0130:hypothetical protein:hypothetical protein:->->:122002..122171 170 ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0129:PI0130|PI0129:PI0130:hypothetical protein:hypothetical protein:->->:122002..122171 170 ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 54 End: 152 ..................................................... M L Q W G M C S E T F C L S N Y L H V K K Y L F T C M E I I T Y V .................. Protein_Len: 50 Strand: + Start: 20 End: 169 ................... M F Y S I I L P L F F Y F T V G Y V F R N V L S V E L L T R K E I F I Y V Y G N Y Y L R V E K H F F . ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0129:PI0130|PI0129:PI0130:hypothetical protein:hypothetical protein:->->:122002..122171 170 ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Intra-Species Hit: Count: 1 Min: 1 Max: 170 Len: 170 Subject: pint_PI0129_PI0130|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[122002,122171]|170 HSP 1 e-value: 7.0E-92 bit: 337.0 Len: 170 Query Start:1 Query End:170 Subject Strand: POSITIVE Subject Start: 1 Subject End: 170 ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta ccctcttaaacggaaggttttgttttatagcataatattgccccttttcttctattttacagtggggtatgtgttccgaaacgttttgtctgtcgaattacttacacgtaaagaaatatttatttacgtgtatggaaattattacttacgtgtagaaaaacatttcttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCCUCUUAAACGGAAGGUUUUGUUUUAUAGCAUAAUAUUGCCCCUUUUCUUCUAUUUUACAGUGGGGUAUGUGUUCCGAAACGUUUUGUCUGUCGAAUUACUUACACGUAAAGAAAUAUUUAUUUACGUGUAUGGAAAUUAUUACUUACGUGUAGAAAAACAUUUCUUUA .........(((((......((((((..((((((....((((((...................))))))))))))..)))))).....)))))...............(((((((((.(((.((((((((((.(..........).)))))))))).))).))))))))) (-34.51) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGAAAUGUUUUUCUACACGUAAGUAAUAAUUUCCAUACACGUAAAUAAAUAUUUCUUUACGUGUAAGUAAUUCGACAGACAAAACGUUUCGGAACACAUACCCCACUGUAAAAUAGAAGAAAAGGGGCAAUAUUAUGCUAUAAAACAAAACCUUCCGUUUAAGAGGG ....(((((((((((((..((....))...........((((((((((.((....)).))))))))))...........))).))))))))))...........(((.((...(((..((((......((((......))))............))))..))).)).))) (-32.67) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 590 130 84 113 67 pint:PI0579|5end_hypothetical_protein_523419..523649_POSITIVE 548 150 102 133 85 pint:PI0648|5end_hypothetical_protein_589448..589678_POSITIVE 528 150 102 111 63 pint:PI1462|5end_hypothetical_protein_1385094..1385324_POSITIVE 474 138 94 211 168 pint:PI0702|5end_conserved_hypothetical_protein;_possible_tetracenomycin_C_synthesis_protein_homolog_638640..638870_POSITIVE 449 150 101 148 99 pint:PI0983|5end_hypothetical_protein_909750..909980_POSITIVE 426 129 86 114 69 pint:PI0535|5end_hypothetical_protein_489508..489738_POSITIVE 413 120 71 81 31 pint:PI1450|5end_hypothetical_protein_1372683..1372913_POSITIVE 392 150 101 155 107 pint:PI1673|5end_hypothetical_protein_1587718..1587948_POSITIVE 387 168 123 231 189 pint:PI0917|5end_phosphoglycolate_phosphatase_846371..846601_POSITIVE 370 137 98 176 137 pint:PI0619|5end_hypothetical_protein_569364..569594_POSITIVE 368 132 101 133 101 pint:PI0312|5end_hypothetical_protein_294591..294821_POSITIVE 327 90 47 216 168 pint:PI0796|5end_competence_protein_733324..733554_POSITIVE 320 119 78 231 190 pint:PI0592|5end_DNA_mismatch_repair_protein_540795..541025_POSITIVE 318 130 88 158 116 pint:PI0115|5end_hypothetical_protein_108062..108292_POSITIVE 318 130 88 144 102 pint:PI0111|5end_hypothetical_protein_108048..108278_POSITIVE 318 130 88 109 67 pint:PI0112|5end_hypothetical_protein_108013..108243_POSITIVE 300 114 71 203 153 pint:PI0750|5end_zinc_protease_677746..677976_POSITIVE 300 120 73 225 177 pint:PI0114|5end_hypothetical_protein_108139..108369_POSITIVE 290 88 42 167 120 pint:PI1628|5end_hypothetical_protein_1551082..1551312_POSITIVE 279 92 56 192 153 pint:PI1860|5end_hypothetical_protein_1805949..1806179_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 464 160 119 51 10 pint:PI0533|3end_ABC_transporter,_permease_protein_488421..488571_POSITIVE 429 156 111 47 2 pint:PI0701|3end_hypothetical_protein_638537..638687_POSITIVE 426 145 102 62 19 pint:PI1314|3end_hypothetical_protein_1231423..1231573_POSITIVE 335 160 114 106 60 pint:PI0725|3end_hypothetical_protein_658933..659083_POSITIVE 300 109 63 134 76 pint:PI0531|3end_ABC_transporter,_ATP-binding_protein;_possible_gliding_motility_related_486239..486389_POSITIVE 292 48 1 84 44 pint:PI1621|3end_hypothetical_protein_1545740..1545890_POSITIVE 287 48 1 84 44 pint:PI0327|3end_hypothetical_protein_309107..309257_POSITIVE 274 156 111 47 2 pint:PI0797|3end_tRNA_pseudouridine_synthase_A_(pseudouridylate_synthase)_735752..735902_POSITIVE 265 47 2 92 37 pint:PI2039|3end_hypothetical_protein_1992991..1993141_POSITIVE 261 95 62 127 91 pint:PI0242|3end_hypothetical_protein_240227..240377_POSITIVE 258 94 60 79 42 pint:PI2080|3end_hypothetical_protein_2038298..2038448_POSITIVE 258 80 35 124 69 pint:PI1807|3end_hypothetical_protein_1742481..1742631_POSITIVE 254 139 105 138 102 pint:PI2093|3end_hypothetical_protein_2049440..2049590_POSITIVE 254 139 105 104 68 pint:PI2092|3end_hypothetical_protein_2049406..2049556_POSITIVE 251 90 43 141 96 pint:PI1837|3end_aspartate_ammonia-lyase_1780406..1780556_POSITIVE 245 78 36 111 69 pint:PI1065|3end_hypothetical_protein_983650..983800_POSITIVE 242 95 61 51 11 pint:PI0261|3end_conjugative_transposon_protein,_TraK_254765..254915_POSITIVE 241 132 103 49 20 pint:PI0598|3end_hypothetical_protein_547233..547383_POSITIVE 240 84 43 68 24 pint:PI2075|3end_conserved_hypothetical_protein_2035355..2035505_POSITIVE 239 88 43 148 104 pint:PI0912|3end_conserved_hypothetical_protein_842692..842842_POSITIVE