Origin IGS: tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctaatgatttgttataatggagatatgcggcagtttgccatagtgccttcgtctttgcgtagcattaactttcttcactccttaatgggcgtgtatgctattttactttttgtgtgtatcgtaagccaacacctgcctataggtcgtgtca Mask Tandem Repeat Region ================================================ tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Find is-nt database================================================ Query_seq: PI0102:PI0103|PI0102:PI0103:hypothetical protein:conserved hypothetical protein; possible permease:->->:98125..98275 151 tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0102:PI0103|PI0102:PI0103:hypothetical protein:conserved hypothetical protein; possible permease:->->:98125..98275 151 tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0102:PI0103|PI0102:PI0103:hypothetical protein:conserved hypothetical protein; possible permease:->->:98125..98275 151 tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Predict ORF larger than 30AA ================================================ Protein_Len: 49 Strand: - Start: 2 End: 148 . V R G I P L H Q S V I C V F L L I A Y V G M L S H L F N I S R L S S P V I A F Q R M D G N Y C I M ... tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0102:PI0103|PI0102:PI0103:hypothetical protein:conserved hypothetical protein; possible permease:->->:98125..98275 151 tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Intra-Species Hit: Count: 1 Min: 1 Max: 151 Len: 151 Subject: pint_PI0102_PI0103|hypothetical protein:conserved hypothetical protein; possible permease|POSITIVE:POSITIVE|[98125,98275]|151 HSP 1 e-value: 1.0E-80 bit: 299.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag tgacacgacctataggcaggtgttggcttacgatacacacaaaaagtaaaatagcatacacgcccattaaggagtgaagaaagttaatgctacgcaaagacgaaggcactatggcaaactgccgcatatctccattataacaaatcattag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACACGACCUAUAGGCAGGUGUUGGCUUACGAUACACACAAAAAGUAAAAUAGCAUACACGCCCAUUAAGGAGUGAAGAAAGUUAAUGCUACGCAAAGACGAAGGCACUAUGGCAAACUGCCGCAUAUCUCCAUUAUAACAAAUCAUUAG ...(((..(((((.(((..((((((..((((..............))))..))))))....))).))..))).)))......((((.((((.((......))..))))....(((.....)))..............)))).......... (-25.74) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAAUGAUUUGUUAUAAUGGAGAUAUGCGGCAGUUUGCCAUAGUGCCUUCGUCUUUGCGUAGCAUUAACUUUCUUCACUCCUUAAUGGGCGUGUAUGCUAUUUUACUUUUUGUGUGUAUCGUAAGCCAACACCUGCCUAUAGGUCGUGUCA ...(((((((........((((......(((.....))).((((((...(((....)))..))))))..........))))...((((((((((..(((((..(((.........)))..)).)))..))))..))))))))))))).... (-35.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 368 117 71 71 11 pint:PI0780|5end_DNA_gyrase,_subunit_A_(DNA_topoisomerase_(ATP-hydrolyzing))_715902..716132_POSITIVE 339 126 86 61 16 pint:PI1676|5end_phosphatidate_cytidylyltransferase_1590896..1591126_POSITIVE 334 130 91 43 9 pint:PI0751|5end_conserved_hypothetical_protein_680623..680853_POSITIVE 327 124 87 115 75 pint:PI0529|5end_hypothetical_protein_483026..483256_POSITIVE 312 126 88 83 35 pint:PI0668|5end_peptidyl-prolyl_cis-trans_isomerase_SlyD,_FKBP-type_(peptidylprolyl_isomerase)_610216..610446_POSITIVE 306 135 94 79 43 pint:PI0009|5end_NAD-dependent_epimerase/dehydratase_family_protein_9653..9883_POSITIVE 304 126 81 67 16 pint:PI0774|5end_phosphoserine_aminotransferase_(phosphoserine_transaminase)_709497..709727_POSITIVE 299 96 54 109 61 pint:PI0690|5end_uracil_permease_626025..626255_POSITIVE 291 130 83 196 151 pint:PI1822|5end_ABC_transporter,_ATP-binding_/_permease_1761452..1761682_POSITIVE 287 130 83 188 131 pint:PI0683|5end_hypothetical_protein_619292..619522_POSITIVE 287 128 83 180 143 pint:PI0528|5end_hypothetical_protein_482791..483021_POSITIVE 285 128 100 166 139 pint:PI1189|5end_conserved_hypothetical_protein_1107114..1107344_POSITIVE 284 109 62 188 132 pint:PI2006|5end_potassium_uptake_system_protein_1964480..1964710_POSITIVE 283 126 98 120 94 pint:PI0171|5end_hypothetical_protein_163850..164080_POSITIVE 282 107 61 216 176 pint:PI1419|5end_conserved_hypothetical_protein;_membrane_protease_subunits,_stomatin/prohibitin_homologs_1339136..1339366_POSITIVE 281 128 89 109 74 pint:PI0073|5end_dihydropteroate_synthase_70956..71186_POSITIVE 280 108 61 111 47 pint:PI0645|5end_hypothetical_protein_586419..586649_POSITIVE 280 108 61 90 26 pint:PI0643|5end_hypothetical_protein_586398..586628_POSITIVE 278 108 62 132 92 pint:PI1009|5end_cytochrome_c_nitrite_reductase,_large_subunit_929342..929572_POSITIVE 278 110 69 154 113 pint:PI0996|5end_hypothetical_protein_920458..920688_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 344 124 89 136 98 pint:PI2025|3end_conserved_hypothetical_protein_1979452..1979602_POSITIVE 327 124 87 131 91 pint:PI0528|3end_hypothetical_protein_483122..483272_POSITIVE 324 100 51 115 39 pint:PI1535|3end_response_regulator_1455501..1455651_POSITIVE 303 100 53 147 103 pint:PI0803|3end_DNA-damage-inducible_protein_F_741171..741321_POSITIVE 287 130 83 72 15 pint:PI0681|3end_hypothetical_protein_619256..619406_POSITIVE 285 150 101 122 58 pint:PI0183|3end_hypothetical_protein_174810..174960_POSITIVE 283 126 98 81 55 pint:PI0170|3end_hypothetical_protein_163891..164041_POSITIVE 281 128 89 65 30 pint:PI0072|3end_conserved_hypothetical_protein_70992..71142_POSITIVE 278 108 62 41 1 pint:PI1008|3end_cytochrome_c_nitrite_reductase,_small_subunit_929331..929481_POSITIVE 276 135 92 47 2 pint:PI1943|3end_hypothetical_protein_1902206..1902356_POSITIVE 274 40 1 120 73 pint:PI0788|3end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726789..726939_POSITIVE 269 124 81 142 89 pint:PI0782|3end_beta-lactamase_induction_signal_transducer,_AmpG_protein_721927..722077_POSITIVE 266 133 94 66 17 pint:PI0838|3end_DNA_replication_and_repair_protein_773927..774077_POSITIVE 265 130 89 129 76 pint:PI2137|3end_RNA_methylase_SpoU_family_2083807..2083957_POSITIVE 263 127 88 77 37 pint:PI1647|3end_hypothetical_protein_1562531..1562681_POSITIVE 263 128 93 150 109 pint:PI0873|3end_conserved_hypothetical_protein;_possible_permease_808956..809106_POSITIVE 263 127 88 77 37 pint:PI0353|3end_hypothetical_protein_325337..325487_POSITIVE 263 125 88 73 35 pint:PI0300|3end_transposase_285761..285911_POSITIVE 262 38 1 78 28 pint:PI1777|3end_conserved_hypothetical_protein_1710081..1710231_POSITIVE 261 126 83 114 57 pint:PI0060|3end_long-chain-fatty-acid--CoA_ligase_(acyl-CoA_synthetase)_60166..60316_POSITIVE