Origin IGS: ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agaagcggaataaggtgaagaaaaacagaaaaaatggagcttacaaagataaataatagaatttatgaaaataaaataaggttatatcaaagcaaataagtacatttgcagtcgtatacttttgtttgacaacgtacaaggtaagcgcgcctatgcatacgtattaacaaacagaaaaacag Mask Tandem Repeat Region ================================================ ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct Find is-nt database================================================ Query_seq: PI0101:PI0102|PI0101:PI0102:lauroyl/myristoyl acyltransferase:hypothetical protein:->->:97814..97995 182 ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0101:PI0102|PI0101:PI0102:lauroyl/myristoyl acyltransferase:hypothetical protein:->->:97814..97995 182 ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0101:PI0102|PI0101:PI0102:lauroyl/myristoyl acyltransferase:hypothetical protein:->->:97814..97995 182 ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 1 End: 93 M F F C L L I R M H R R A Y L V R C Q T K V Y D C K C T Y L L ......................................................................................... Protein_Len: 37 Strand: + Start: 29 End: 139 ............................ M G A L T L Y V V K Q K Y T T A N V L I C F D I T L F Y F H K F Y Y L S L ........................................... Protein_Len: 32 Strand: - Start: 2 End: 97 . T K R N T L V Y A Y A R K G Q V N D F L L I R S C I Y K N A K M ..................................................................................... ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0101:PI0102|PI0101:PI0102:lauroyl/myristoyl acyltransferase:hypothetical protein:->->:97814..97995 182 ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataaccttattttattttcataaattctattatttatctttgtaagctccattttttctgtttttcttcaccttattccgcttct Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: pint_PI0101_PI0102|lauroyl/myristoyl acyltransferase:hypothetical protein|POSITIVE:POSITIVE|[97814,97995]|182 HSP 1 e-value: 7.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataacc............................................................................... ctgtttttctgtttgttaatacgtatgcataggcgcgcttaccttgtacgttgtcaaacaaaagtatacgactgcaaatgtacttatttgctttgatataacc............................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGUUUUUCUGUUUGUUAAUACGUAUGCAUAGGCGCGCUUACCUUGUACGUUGUCAAACAAAAGUAUACGACUGCAAAUGUACUUAUUUGCUUUGAUAUAACC .........((((((.....((((((....(((........))).))))))...))))))...((((.(((..(((((((....))))))).))))))).... (-20.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUAUAUCAAAGCAAAUAAGUACAUUUGCAGUCGUAUACUUUUGUUUGACAACGUACAAGGUAAGCGCGCCUAUGCAUACGUAUUAACAAACAGAAAAACAG ...(((((.(..((((((......))))))..).))))).(((((((((...(((((((((((......)))).))..))))).....)))))))))...... (-20.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 43 3 211 174 pint:PI1000|5end_conserved_hypothetical_protein_923332..923562_POSITIVE 314 54 11 223 162 pint:PI2169|5end_hypothetical_protein_2111706..2111936_POSITIVE 314 54 11 107 46 pint:PI2167|5end_hypothetical_protein_2111590..2111820_POSITIVE 310 59 14 172 133 pint:PI0817|5end_glycosyl_transferase,_group_1_family_protein_756861..757091_POSITIVE 310 70 26 65 26 pint:PI0814|5end_conserved_hypothetical_protein_752946..753176_POSITIVE 306 57 22 45 8 pint:PI1791|5end_conserved_hypothetical_protein_1718489..1718719_POSITIVE 302 59 19 66 18 pint:PI0028|5end_aminopeptidase_31559..31789_POSITIVE 297 56 20 222 187 pint:PI0537|5end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_489756..489986_POSITIVE 284 75 32 208 148 pint:PI0672|5end_conserved_hypothetical_protein_611746..611976_POSITIVE 279 70 23 219 174 pint:PI1172|5end_sensor_histidine_kinase_1091850..1092080_POSITIVE 277 54 11 177 137 pint:PI1831|5end_conserved_hypothetical_protein_1770298..1770528_POSITIVE 273 38 9 161 131 pint:PI1754|5end_conserved_hypothetical_protein_1681202..1681432_POSITIVE 272 57 23 37 1 pint:PI1788|5end_hypothetical_protein_1718481..1718711_POSITIVE 270 50 1 231 185 pint:PI2005|5end_potassium_uptake_protein_1962950..1963180_POSITIVE 270 55 12 182 143 pint:PI0707|5end_conserved_hypothetical_protein;_possible_integral_membrane_protein_643358..643588_POSITIVE 270 35 1 68 30 pint:PI0108|5end_L-lactate_permease_104892..105122_POSITIVE 267 50 2 141 90 pint:PI1980|5end_conserved_hypothetical_protein_1937470..1937700_POSITIVE 267 64 21 192 152 pint:PI1078|5end_conserved_hypothetical_protein_999500..999730_POSITIVE 267 80 31 74 21 pint:PI0250|5end_hypothetical_protein_246946..247176_POSITIVE 266 72 31 63 22 pint:PI0752|5end_conserved_hypothetical_protein_681882..682112_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 43 3 135 98 pint:PI0998|3end_possible_flavin_reductase_domain_protein_923336..923486_POSITIVE 310 59 14 151 112 pint:PI0816|3end_glycogen_debranching_enzyme_756920..757070_POSITIVE 295 60 11 81 32 pint:PI0101|3end_lauroyl/myristoyl_acyltransferase_97753..97903_POSITIVE 289 60 11 128 83 pint:PI0889|3end_pyruvate_kinase_820355..820505_POSITIVE 280 36 1 150 108 pint:PI1245|3end_hypothetical_protein_1171492..1171642_POSITIVE 279 70 23 125 80 pint:PI1171|3end_hypothetical_protein_1091836..1091986_POSITIVE 276 47 2 104 59 pint:PI1862|3end_hypothetical_protein_1806391..1806541_POSITIVE 276 47 1 149 105 pint:PI1514|3end_tRNA_nucleotidyltransferase/poly(A)_polymerase_1434072..1434222_POSITIVE 273 38 9 149 119 pint:PI1752|3end_conserved_hypothetical_protein_1681270..1681420_POSITIVE 270 55 12 138 99 pint:PI0706|3end_pyridoxal_phosphate_biosynthetic_protein_(4-hydroxythreonine-4-phosphate_dehydrogenase)_643394..643544_POSITIVE 267 80 31 74 21 pint:PI0249|3end_conserved_hypothetical_protein_247026..247176_POSITIVE 263 55 23 129 94 pint:PI0675|3end_hypothetical_protein_613957..614107_POSITIVE 260 70 22 66 8 pint:PI0704|3end_conserved_hypothetical_protein_641364..641514_POSITIVE 258 77 31 70 24 pint:PI0117|3end_ribonuclease_HI_111314..111464_POSITIVE 257 54 21 132 101 pint:PI0896|3end_2-oxoglutarate_oxidoreductase,_alpha_subunit_828276..828426_POSITIVE 255 66 27 147 99 pint:PI0028|3end_aminopeptidase_32727..32877_POSITIVE 253 43 1 143 101 pint:PI2103|3end_hypothetical_protein_2057934..2058084_POSITIVE 252 55 26 151 126 pint:PI1629|3end_hypothetical_protein_1551966..1552116_POSITIVE 251 55 26 48 12 pint:PI1825|3end_2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase_1765705..1765855_POSITIVE 251 49 1 96 44 pint:PI0297|3end_hypothetical_protein_277916..278066_POSITIVE