Origin IGS: agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagacaggtttggatagccccgtaatgagcagaaaagaggcaaaatacgtgtaaatatttttcacaagcagaatcgttacgtaacaaccttactatcaatact Mask Tandem Repeat Region ================================================ agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Find is-nt database================================================ Query_seq: PI0089:PI0090|PI0089:PI0090:hypothetical protein:amidophosphoribosyltransferase:->->:88416..88518 103 agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0089:PI0090|PI0089:PI0090:hypothetical protein:amidophosphoribosyltransferase:->->:88416..88518 103 agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0089:PI0090|PI0089:PI0090:hypothetical protein:amidophosphoribosyltransferase:->->:88416..88518 103 agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 5 End: 103 .... M I V R L L R N D S A C E K Y L H V F C L F S A H Y G A I Q T C L agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0089:PI0090|PI0089:PI0090:hypothetical protein:amidophosphoribosyltransferase:->->:88416..88518 103 agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: pint_PI0089_PI0090|hypothetical protein:amidophosphoribosyltransferase|POSITIVE:POSITIVE |[88416,88518]|103 HSP 1 e-value: 4.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta agtattgatagtaaggttgttacgtaacgattctgcttgtgaaaaatatttacacgtattttgcctcttttctgctcattacggggctatccaaacctgtcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAUUGAUAGUAAGGUUGUUACGUAACGAUUCUGCUUGUGAAAAAUAUUUACACGUAUUUUGCCUCUUUUCUGCUCAUUACGGGGCUAUCCAAACCUGUCUA ......(((((...((..((..(((((......(((.((((((.....)))))).)))....((.........))...)))))..))...))....))))).. (-17.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGACAGGUUUGGAUAGCCCCGUAAUGAGCAGAAAAGAGGCAAAAUACGUGUAAAUAUUUUUCACAAGCAGAAUCGUUACGUAACAACCUUACUAUCAAUACU (((..(((((.((....)).((((((((((.........)).......(((.(((....))))))........))))))))....)))))..)))........ (-14.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 485 59 11 123 75 pint:PI0104|5end_hypothetical_protein_99105..99335_POSITIVE 471 75 34 58 19 pint:PI1814|5end_hypothetical_protein_1752953..1753183_POSITIVE 469 78 34 112 68 pint:PI0928|5end_hypothetical_protein_859851..860081_POSITIVE 451 70 21 146 96 pint:PI0200|5end_hypothetical_protein_191545..191775_POSITIVE 421 80 34 145 98 pint:PI0099|5end_hypothetical_protein_95530..95760_POSITIVE 391 76 34 88 46 pint:PI0689|5end_hypothetical_protein_625624..625854_POSITIVE 373 70 21 201 151 pint:PI0841|5end_hypothetical_protein_775406..775636_POSITIVE 363 74 34 107 68 pint:PI1922|5end_hypothetical_protein_1865219..1865449_POSITIVE 359 59 11 136 83 pint:PI0182|5end_hypothetical_protein_174093..174323_POSITIVE 359 70 28 146 100 pint:PI0061|5end_hypothetical_protein_60500..60730_POSITIVE 353 50 2 125 77 pint:PI2131|5end_hypothetical_protein_2080678..2080908_POSITIVE 341 54 14 157 117 pint:PI1694|5end_hypothetical_protein_1609717..1609947_POSITIVE 337 76 36 187 147 pint:PI2128|5end_hypothetical_protein_2076512..2076742_POSITIVE 320 66 27 152 110 pint:PI0755|5end_hypothetical_protein_686691..686921_POSITIVE 305 54 11 129 83 pint:PI1733|5end_hypothetical_protein_1652160..1652390_POSITIVE 300 57 11 160 113 pint:PI1601|5end_hypothetical_protein_1530282..1530512_POSITIVE 296 57 19 48 9 pint:PI0759|5end_hypothetical_protein_693763..693993_POSITIVE 294 76 34 138 98 pint:PI1057|5end_hypothetical_protein_977286..977516_POSITIVE 291 75 33 205 161 pint:PI0851|5end_hypothetical_protein_784310..784540_POSITIVE 290 100 54 64 19 pint:PI1779|5end_16S_rRNA_processing_protein_1711335..1711565_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 471 75 34 111 72 pint:PI1813|3end_hypothetical_protein_1753086..1753236_POSITIVE 421 80 34 67 20 pint:PI0098|3end_conserved_hypothetical_protein_(possible_glycosyl/glycerophosphate_transferase)_95532..95682_POSITIVE 391 76 34 134 92 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 358 55 11 52 8 pint:PI1485|3end_hypothetical_protein_1405339..1405489_POSITIVE 353 50 2 78 30 pint:PI2130|3end_DNA_polymerase_I_2080711..2080861_POSITIVE 352 69 24 48 3 pint:PI0103|3end_conserved_hypothetical_protein;_possible_permease_99100..99250_POSITIVE 321 77 37 75 35 pint:PI0412|3end_primosomal_protein_N',_replication_factor_Y_382152..382302_POSITIVE 310 76 33 86 41 pint:PI0051|3end_hypothetical_protein_53388..53538_POSITIVE 300 57 11 73 26 pint:PI1600|3end_conserved_hypothetical_protein_1530275..1530425_POSITIVE 300 56 19 44 7 pint:PI1424|3end_probable_peptidase_family_M48_1344044..1344194_POSITIVE 290 100 54 48 3 pint:PI1778|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1711399..1711549_POSITIVE 286 87 55 74 36 pint:PI0261|3end_conjugative_transposon_protein,_TraK_254765..254915_POSITIVE 285 87 54 149 115 pint:PI0601|3end_hypothetical_protein_549020..549170_POSITIVE 278 92 55 125 92 pint:PI1690|3end_transcription_regulator;_possible_Fe2+/Zn2+_uptake_regulation_proteins_1605627..1605777_POSITIVE 276 75 33 127 86 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 271 93 56 63 33 pint:PI0042|3end_hypothetical_protein_43444..43594_POSITIVE 270 74 33 62 6 pint:PI0160|3end_conserved_hypothetical_protein_153268..153418_POSITIVE 259 54 17 38 1 pint:PI0770|3end_hypothetical_protein_706354..706504_POSITIVE 257 76 38 77 38 pint:PI2172|3end_hypothetical_protein_2113991..2114141_POSITIVE 257 76 38 50 11 pint:PI2173|3end_hypothetical_protein_2113964..2114114_POSITIVE