Origin IGS: aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attaaatgcacgataaacctcataaagtgtgctgccgattgggacttctaaagtttttttattatttttgcaacggatttgtaatacttgtttcatcttgtatgaacgtttaacgatttaaatcagggtataaagttagcaagttctcattaaagagcaaaaaaaattgcattacaatacaattaatt Mask Tandem Repeat Region ================================================ aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Find is-nt database================================================ Query_seq: PI0074:PI0075|PI0074:PI0075:Na+/phosphate symporter (sodium-dependent inorganic phosphate (Pi) transporter):uridine kinase:->->:73700..73887 188 aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0074:PI0075|PI0074:PI0075:Na+/phosphate symporter (sodium-dependent inorganic phosphate (Pi) transporter):uridine kinase:->->:73700..73887 188 aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0074:PI0075|PI0074:PI0075:Na+/phosphate symporter (sodium-dependent inorganic phosphate (Pi) transporter):uridine kinase:->->:73700..73887 188 aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 72 End: 188 ....................................................................... M V K R S Y K M K Q V L Q I R C K N N K K T L E V P I G S T L Y E V Y R A F N Protein_Len: 35 Strand: - Start: 79 End: 183 .............................................................................. V N M C S S V L I V F G N C F Y Y F F K L L G L R C C V K H P K D H M ..... Protein_Len: 30 Strand: - Start: 71 End: 160 ...................................................................... I T L R E Y L I F C T N C I R Q L F L L F V K S T G I P L M ............................ aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0074:PI0075|PI0074:PI0075:Na+/phosphate symporter (sodium-dependent inorganic phosphate (Pi) transporter):uridine kinase:->->:73700..73887 188 aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Intra-Species Hit: Count: 1 Min: 1 Max: 188 Len: 188 Subject: pint_PI0074_PI0075|Na+/phosphate symporter (sodium-dependent inorganic phosphate (Pi) transporter):uridine kinase|POSITIVE:POSITIVE|[73700,73887]|188 HSP 1 e-value: 1.0E-75 bit: 283.0 Len: 188 Query Start:1 Query End:188 Subject Strand: POSITIVE Subject Start: 1 Subject End: 188 aattaattgtattgtaatgcaannnnnnnngctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataatnnnnnnnctttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat aattaattgtattgtaatgcaattttttttgctctttaatgagaacttgctaactttataccctgatttaaatcgttaaacgttcatacaagatgaaacaagtattacaaatccgttgcaaaaataataaaaaaactttagaagtcccaatcggcagcacactttatgaggtttatcgtgcatttaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUAAUUGUAUUGUAAUGCAAUUUUUUUUGCUCUUUAAUGAGAACUUGCUAACUUUAUACCCUGAUUUAAAUCGUUAAACGUUCAUACAAGAUGAAACAAGUAUUACAAAUCCGUUGCAAAAAUAAUAAAAAAACUUUAGAAGUCCCAAUCGGCAGCACACUUUAUGAGGUUUAUCGUGCAUUUAAU .(((((.(((((.....((((((....(((((((......))).(((((...............(((....)))........(((((.....))))).)))))....))))...))))))............(((((((((((((.((....))......))))).))))))))...))))).))))) (-26.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAAAUGCACGAUAAACCUCAUAAAGUGUGCUGCCGAUUGGGACUUCUAAAGUUUUUUUAUUAUUUUUGCAACGGAUUUGUAAUACUUGUUUCAUCUUGUAUGAACGUUUAACGAUUUAAAUCAGGGUAUAAAGUUAGCAAGUUCUCAUUAAAGAGCAAAAAAAAUUGCAUUACAAUACAAUUAAUU ....((((((...........((((((...(((...((........))...)))..))))))..(((((((...((((((((...((((.....((((((..((((((.....)).))))...))))))...))))..)))))))).(......).)))))))....))))))............... (-29.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 288 180 136 73 25 pint:PI0141|5end_glutamine_amidotransferase_involved_in_pyridoxine_biosynthesis_132710..132940_POSITIVE 278 179 141 180 151 pint:PI0706|5end_pyridoxal_phosphate_biosynthetic_protein_(4-hydroxythreonine-4-phosphate_dehydrogenase)_642226..642456_POSITIVE 271 180 137 203 144 pint:PI2119|5end_conserved_hypothetical_protein;_possible_cytochrome_B_subunit_2068031..2068261_POSITIVE 266 89 47 135 85 pint:PI0478|5end_50S_ribosomal_protein_L30_440923..441153_POSITIVE 261 169 135 141 105 pint:PI0279|5end_hypothetical_protein_265600..265830_POSITIVE 260 178 135 47 5 pint:PI0149|5end_conserved_hypothetical_protein_142735..142965_POSITIVE 252 180 132 59 28 pint:PI2107|5end_hypothetical_protein_2059704..2059934_POSITIVE 249 179 132 122 71 pint:PI1222|5end_hypothetical_protein_1142834..1143064_POSITIVE 245 178 139 104 57 pint:PI1381|5end_conserved_hypothetical_protein_1303060..1303290_POSITIVE 241 70 35 166 122 pint:PI0487|5end_50S_ribosomal_protein_L17_446144..446374_POSITIVE 234 179 143 199 159 pint:PI1936|5end_conserved_hypothetical_protein;_possible_serine-type_protease_1887039..1887269_POSITIVE 233 179 133 91 44 pint:PI0538|5end_transcriptional_regulator_492561..492791_POSITIVE 231 179 135 57 14 pint:PI1630|5end_hypothetical_protein_1552127..1552357_POSITIVE 230 180 136 70 26 pint:PI2067|5end_hypothetical_protein_2027760..2027990_POSITIVE 226 178 135 65 26 pint:PI2123|5end_adenine_phosphoribosyltransferase_2071711..2071941_POSITIVE 222 89 43 228 186 pint:PI0783|5end_hypothetical_protein_723144..723374_POSITIVE 216 91 51 217 175 pint:PI1422|5end_hypothetical_protein_1340690..1340920_POSITIVE 213 179 139 185 140 pint:PI1299|5end_glycosyltransferase_1212191..1212421_POSITIVE 212 55 11 94 44 pint:PI0497|5end_predicted_recombinase,_tnpA_protein_458741..458971_POSITIVE 210 54 24 219 185 pint:PI1947|5end_hypothetical_protein_1902534..1902764_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 278 179 141 145 116 pint:PI0705|3end_conserved_hypothetical_protein;_possible_lipoprotein_642271..642421_POSITIVE 271 180 137 89 30 pint:PI2118|3end_fumarate_reductase/succinate_dehydrogenase_flavoprotein_subunit,_N-terminal_2067997..2068147_POSITIVE 266 89 47 113 63 pint:PI0477|3end_30S_ribosomal_protein_S5_440981..441131_POSITIVE 261 169 135 86 50 pint:PI0278|3end_hypothetical_protein_265625..265775_POSITIVE 254 180 146 93 60 pint:PI0872|3end_hypothetical_protein_807807..807957_POSITIVE 249 179 132 63 12 pint:PI1221|3end_penicillin-binding_protein_1A_1142855..1143005_POSITIVE 244 179 144 100 63 pint:PI1572|3end_hypothetical_protein_1498215..1498365_POSITIVE 241 70 35 90 46 pint:PI0486|3end_DNA-directed_RNA_polymerase,_alpha_subunit_446148..446298_POSITIVE 233 179 133 73 26 pint:PI0536|3end_hypothetical_protein_492623..492773_POSITIVE 218 180 146 64 24 pint:PI1924|3end_cytidine_deaminase_1866495..1866645_POSITIVE 216 119 72 76 30 pint:PI1890|3end_cell_division_protein_FtsW_1834072..1834222_POSITIVE 216 91 51 99 57 pint:PI1420|3end_probable_membrane_protein_1340652..1340802_POSITIVE 215 180 151 139 108 pint:PI1933|3end_hypothetical_protein_1880463..1880613_POSITIVE 212 179 150 146 121 pint:PI0195|3end_epimerase/reductase_188202..188352_POSITIVE 207 180 140 79 27 pint:PI0059|3end_peptide_chain_release_factor_RF-2_58171..58321_POSITIVE 203 178 149 146 113 pint:PI1153|3end_histidine_kinase_sensor_protein_1078638..1078788_POSITIVE 202 60 18 92 55 pint:PI0093|3end_riboflavin_biosynthesis_protein_91537..91687_POSITIVE 201 69 28 93 57 pint:PI1777|3end_conserved_hypothetical_protein_1710081..1710231_POSITIVE 200 174 140 39 11 pint:PI1849|3end_conserved_hypothetical_protein;_possible_acyltransferase_family_protein_1796039..1796189_POSITIVE 200 172 147 75 56 pint:PI0375|3end_hypothetical_protein_346082..346232_POSITIVE