Origin IGS: taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atcgaaattatcgttttcttaacgaattatctttacaaaacaagggctatgctttactatttcaaagcaaaaattctaccaaccgcaacagctatacactaaccgtgaagatgtggctgcggtggcttttttccatctttctta Mask Tandem Repeat Region ================================================ taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Find is-nt database================================================ Query_seq: PI0036:PI0037|PI0036:PI0037:glutathione peroxidase:hypothetical protein:->->:39350..39493 144 taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0036:PI0037|PI0036:PI0037:glutathione peroxidase:hypothetical protein:->->:39350..39493 144 taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0036:PI0037|PI0036:PI0037:glutathione peroxidase:hypothetical protein:->->:39350..39493 144 taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 46 End: 144 ............................................. H I A T A T P L I K A K F Y Y L M A R T K Y L Y N T L F V I I E M Protein_Len: 39 Strand: - Start: 3 End: 119 .. S L H F F A V A A V D E R N T Y L Q Q P Q Y F K Q K S I T F C L G Q K T F I M ......................... taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 85 End: 114 ....................................................................................CTTTACAAAACAAGGGCTATGCTTTACTAT.............................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0036:PI0037|PI0036:PI0037:glutathione peroxidase:hypothetical protein:->->:39350..39493 144 taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Intra-Species Hit: Count: 1 Min: 1 Max: 144 Len: 144 Subject: pint_PI0036_PI0037|glutathione peroxidase:hypothetical protein|POSITIVE:POSITIVE|[39350,39493]|144 HSP 1 e-value: 2.0E-76 bit: 285.0 Len: 144 Query Start:1 Query End:144 Subject Strand: POSITIVE Subject Start: 1 Subject End: 144 taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat taagaaagatggaaaaaagccaccgcagccacatcttcacggttagtgtatagctgttgcggttggtagaatttttgctttgaaatagtaaagcatagcccttgttttgtaaagataattcgttaagaaaacgataatttcgat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAAAGAUGGAAAAAAGCCACCGCAGCCACAUCUUCACGGUUAGUGUAUAGCUGUUGCGGUUGGUAGAAUUUUUGCUUUGAAAUAGUAAAGCAUAGCCCUUGUUUUGUAAAGAUAAUUCGUUAAGAAAACGAUAAUUUCGAU ...((((...((......(((((((((((........(((.....))).......))))))).))))........(((((((......)))))))...)).(((((((.(((.((....)).)))..)))))))...))))... (-32.16) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCGAAAUUAUCGUUUUCUUAACGAAUUAUCUUUACAAAACAAGGGCUAUGCUUUACUAUUUCAAAGCAAAAAUUCUACCAACCGCAACAGCUAUACACUAACCGUGAAGAUGUGGCUGCGGUGGCUUUUUUCCAUCUUUCUUA ...((((...(((((.....)))))..................(((...((((((........)))))).............((((..(((((((((((.....)))....))))))))..))))......)))...))))... (-29.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 456 139 91 204 151 pint:PI0056|5end_conserved_hypothetical_protein;_possible_DNA_uptake_protein_54967..55197_POSITIVE 428 139 93 52 1 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 396 70 21 214 148 pint:PI1653|5end_hypothetical_protein_1565158..1565388_POSITIVE 368 68 21 189 133 pint:PI0959|5end_uridine_kinase_890495..890725_POSITIVE 352 140 92 230 179 pint:PI0183|5end_hypothetical_protein_174347..174577_POSITIVE 336 140 91 136 87 pint:PI1870|5end_hypothetical_protein_1815302..1815532_POSITIVE 324 78 36 50 11 pint:PI0405|5end_hypothetical_protein_372534..372764_POSITIVE 319 59 11 205 155 pint:PI0667|5end_hypothetical_protein_610113..610343_POSITIVE 315 66 23 184 136 pint:PI1493|5end_conserved_hypothetical_protein_1412268..1412498_POSITIVE 313 139 99 211 169 pint:PI0875|5end_conserved_hypothetical_protein_810608..810838_POSITIVE 312 69 23 226 177 pint:PI0418|5end_hypothetical_protein_386666..386896_POSITIVE 312 69 23 111 62 pint:PI0420|5end_hypothetical_protein_386551..386781_POSITIVE 312 69 23 97 48 pint:PI0417|5end_hypothetical_protein_386537..386767_POSITIVE 306 100 53 71 26 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 306 100 53 79 34 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 306 100 53 71 26 pint:PI1167|5end_hypothetical_protein_1089688..1089918_POSITIVE 305 56 23 227 195 pint:PI0537|5end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_489756..489986_POSITIVE 305 139 91 183 138 pint:PI0202|5end_DNA_helicase_191753..191983_POSITIVE 304 67 22 157 109 pint:PI1141|5end_hypothetical_protein_1069463..1069693_POSITIVE 304 68 21 208 148 pint:PI0737|5end_hypothetical_protein_671182..671412_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 373 140 93 86 43 pint:PI0670|3end_hypothetical_protein_611280..611430_POSITIVE 358 116 72 128 82 pint:PI1679|3end_hypothetical_protein_1594964..1595114_POSITIVE 352 140 92 80 29 pint:PI0182|3end_hypothetical_protein_174277..174427_POSITIVE 347 140 100 138 97 pint:PI1129|3end_hypothetical_protein_1055603..1055753_POSITIVE 343 140 98 107 65 pint:PI1905|3end_hypothetical_protein_1847854..1848004_POSITIVE 331 68 24 91 48 pint:PI0654|3end_K+-dependent_Na+/Ca+_exchanger_related-protein_597223..597373_POSITIVE 318 140 91 86 39 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 317 139 98 99 58 pint:PI0755|3end_hypothetical_protein_686884..687034_POSITIVE 315 66 23 146 98 pint:PI1492|3end_triosephosphate_isomerase_1412310..1412460_POSITIVE 314 66 23 85 52 pint:PI1651|3end_hypothetical_protein_1564512..1564662_POSITIVE 310 70 25 114 79 pint:PI1983|3end_possible_xylanase_1940004..1940154_POSITIVE 305 139 91 85 40 pint:PI0200|3end_hypothetical_protein_191735..191885_POSITIVE 298 79 32 125 69 pint:PI1143|3end_hypothetical_protein_1070504..1070654_POSITIVE 297 140 95 126 81 pint:PI1167|3end_hypothetical_protein_1089890..1090040_POSITIVE 293 129 97 151 116 pint:PI2153|3end_hypothetical_protein_2099630..2099780_POSITIVE 291 69 22 75 8 pint:PI0157|3end_hypothetical_protein_151387..151537_POSITIVE 290 139 91 151 102 pint:PI1281|3end_hypothetical_protein_1196991..1197141_POSITIVE 287 56 19 138 93 pint:PI0087|3end_conserved_hypothetical_protein_86733..86883_POSITIVE 285 70 24 49 5 pint:PI0520|3end_hypothetical_protein_478951..479101_POSITIVE 283 69 23 63 23 pint:PI1649|3end_hypothetical_protein_1563441..1563591_POSITIVE