Origin IGS: taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagtataactattgtataacaatctgtatatcaacgtattgtaaaacctattgttttgcattccaaaagcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaacagccgctttcgcaatgccaaatcgaaattatcgtttttcagcgaaattatctttacaaaaccgaggcgattttcccacgtgttggctgaaagtttctagaaatcctagataaactagaattactatataacccctttcttattcagtgccaggaaacgcaataaaccacgcacctacctacactgagcggcataaaagcataaaaaaacgtgtagtcctgataggcacaatgccattcggaactacacgttttttatgttctgtacgctgttta Mask Tandem Repeat Region ================================================ taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Find is-nt database================================================ Query_seq: PI0023:PI0024|PI0023:PI0024:internalin-related protein:hypothetical protein:->->:28131..28517 387 taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0023:PI0024|PI0023:PI0024:internalin-related protein:hypothetical protein:->->:28131..28517 387 taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0023:PI0024|PI0023:PI0024:internalin-related protein:hypothetical protein:->->:28131..28517 387 taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 149 End: 244 .................................................................................................................................................... M V I L V Y L G F L E T F S Q H V G K S P R F C K D N F A E K R ............................................................................................................................................... Protein_Len: 37 Strand: + Start: 244 End: 354 ................................................................................................................................................................................................................................................... M I S I W H C E S G C F A T Q N L R F Y R A K Q P L L E C K T I G F T I R ................................. Protein_Len: 54 Strand: + Start: 225 End: 386 ................................................................................................................................................................................................................................ M I S L K N D N F D L A L R K R L F C N A K P T L L P C K T A A F G M Q N N R F Y N T L I Y R L L Y N S Y T . Protein_Len: 53 Strand: - Start: 179 End: 337 .................................................................................................................................................................................. F S E A L V H S F R R P K T F I I E S F F S L K S K A N R F R S N Q L A F G V S K G H L V A A K P I C F M .................................................. Protein_Len: 41 Strand: - Start: 166 End: 288 ..................................................................................................................................................................... R P N R S V K L W C T P F D G R N Q L S L K A S F R Y N R N P M A F A A T K C R M ................................................................................................... Protein_Len: 43 Strand: - Start: 1 End: 129 L C R V S C L F R T T G F P M T G I L V V R K K I S K H R E T Y T P A H N I A N G P M .................................................................................................................................................................................................................................................................. taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 321 End: 350 ................................................................................................................................................................................................................................................................................................................................TTTGGAATGCAAAACAATAGGTTTTACAAT..................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -13.3 Tail_Score: -5.77632 Start: 26 End: 66 Full_Region: ACAGAACATAAAAAA CGTGTAGTTCCGAATGGCA TTG TGCCTATCAGGACTACACG TTTTTTTATGCTTTT .........................CGTGTAGTTCCGAATGGCATTGTGCCTATCAGGACTACACG................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -13.3 Tail_Score: -5.53436 Start: 26 End: 66 Full_Region: aaaagcataaaaaaa cgtgtagtcctgataggca caa tgccattcggaactacacg ttttttatgttctgt .........................cgtgtagtcctgataggcacaatgccattcggaactacacg................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -17.0 Tail_Score: -4.13767 Start: 269 End: 319 Full_Region: TTTGGCATTGCGAAA GCGGCTGTTTTGCAACGCAAAACC TAC GCTTTTACCGTGCAAAACAGCCGC TTTTGGAATGCAAAA ............................................................................................................................................................................................................................................................................GCGGCTGTTTTGCAACGCAAAACCTACGCTTTTACCGTGCAAAACAGCCGC.................................................................... TransTerm Strand: - Conf: 100 HP_score: -19.2 Tail_Score: -3.57144 Start: 269 End: 319 Full_Region: ttttgcattccaaaa gcggctgttttgcacggtaaaagc gta ggttttgcgttgcaaaacagccgc tttcgcaatgccaaa ............................................................................................................................................................................................................................................................................gcggctgttttgcacggtaaaagcgtaggttttgcgttgcaaaacagccgc.................................................................... Find igs database================================================ Query_seq: PI0023:PI0024|PI0023:PI0024:internalin-related protein:hypothetical protein:->->:28131..28517 387 taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Intra-Species Hit: Count: 52 Min: 1 Max: 387 Len: 387 Subject: pint_PI0023_PI0024|internalin-related protein:hypothetical protein|POSITIVE:POSITIVE|[28131,28517]|387 HSP 1 e-value: 0.0 bit: 726.0 Len: 387 Query Start:1 Query End:387 Subject Strand: POSITIVE Subject Start: 1 Subject End: 387 taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgnnnnnnnatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt taaacagcgtacagaacataaaaaacgtgtagttccgaatggcattgtgcctatcaggactacacgtttttttatgcttttatgccgctcagtgtaggtaggtgcgtggtttattgcgtttcctggcactgaataagaaaggggttatatagtaattctagtttatctaggatttctagaaactttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagttatactt Subject: pint_PI1563_PI1564|thiol:disulfide interchange protein dsbD:surface antigen BspA|POSITIVE:POSITIVE|[1482876,1483594]|719 HSP 1 e-value: 4.0E-55 bit: 216.0 Len: 145 Query Start:216 Query End:360 Subject Strand: NEGATIVE Subject Start: 372 Subject End: 516 .......................................................................................................................................................................................................................tttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatat........................... .......................................................................................................................................................................................................................tttgtaaagataattctgatgaaaaatgataatttcgatttggcattgcgaaagcggctgttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttgtaatgcgttgatat........................... Subject: pint_PI2088_PI2089|conserved hypothetical protein; possible PAP2 superfamily protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2045876,2046339]|464 HSP 1 e-value: 3.0E-53 bit: 210.0 Len: 164 Query Start:183 Query End:346 Subject Strand: POSITIVE Subject Start: 125 Subject End: 286 ......................................................................................................................................................................................ctttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctga-aaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... ......................................................................................................................................................................................ctttaagccaacacgtg--aaaatcgcttcgattttgtaaagataatt-cgttaagaaaacgataatttcgatttgacgttgcgaaagctgctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... Subject: pint_PI0574_PI0575|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[519505,519700]|196 HSP 1 e-value: 4.0E-52 bit: 206.0 Len: 168 Query Start:214 Query End:381 Subject Strand: POSITIVE Subject Start: 9 Subject End: 176 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagtt...... .....................................................................................................................................................................................................................gttttgtaaagataattccgaagaaaaatgataatttcgatttgacgttgcgaaagtggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttgcaatgcaaaacaataggttttgtaatgcgttgatagaaagagagttatacaatagtt...... Subject: pint_PI1332_PI1333|aminotransferase, possible pyridoxal-phosphate-dependent aminotransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[1250627,1250916]|290 HSP 1 e-value: 2.0E-51 bit: 204.0 Len: 143 Query Start:215 Query End:357 Subject Strand: POSITIVE Subject Start: 91 Subject End: 233 ......................................................................................................................................................................................................................ttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttga.............................. ......................................................................................................................................................................................................................ttttgtaaagataatttctttaaaaagtaataatttcgatttgacattgcgaaaacggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggagtgcaaaacaataggttttgcaatacgttga.............................. Subject: pint_PI0694_PI0695|Na+/dicarboxylate or sulfate symporter:xanthine/uracil permease family protein|POSITIVE:POSITIVE|[631238,631552]|315 HSP 1 e-value: 4.0E-49 bit: 196.0 Len: 139 Query Start:207 Query End:345 Subject Strand: POSITIVE Subject Start: 80 Subject End: 218 ..............................................................................................................................................................................................................cgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... ..............................................................................................................................................................................................................cgccttggttttgtaaagataattccgacggaaaacggtaatttcgatttgacactgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaatgcaaaacaataggtttt.......................................... Subject: pint_PI1930_PI1931|conserved hypothetical protein; possible ankyrin repeat protein (eukaryotic-like):alpha glycan phosphorylase, maltodextrin phosphorylase|POSITIVE:POSITIVE|[1875115,1875595]|481 HSP 1 e-value: 4.0E-46 bit: 186.0 Len: 146 Query Start:214 Query End:359 Subject Strand: POSITIVE Subject Start: 112 Subject End: 257 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ .....................................................................................................................................................................................................................gttttgtaaagataatttctttgaaaaatggtaatttcgatttgacgttgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggttttgtaatgcgttgata............................ Subject: pint_PI1803_PI1804|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1737852,1738248]|397 HSP 1 e-value: 2.0E-45 bit: 184.0 Len: 109 Query Start:238 Query End:346 Subject Strand: POSITIVE Subject Start: 289 Subject End: 397 .............................................................................................................................................................................................................................................aaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... .............................................................................................................................................................................................................................................aaaacggtaatttcgatttggcattgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttacaatgcaaaacaataggtttta......................................... Subject: pint_PI1051_PI1052|hypothetical protein:fructose-1, 6-bisphosphatase|POSITIVE:POSITIVE|[970289,970631]|343 HSP 1 e-value: 2.0E-45 bit: 184.0 Len: 133 Query Start:213 Query End:345 Subject Strand: NEGATIVE Subject Start: 41 Subject End: 173 ....................................................................................................................................................................................................................ggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... ....................................................................................................................................................................................................................ggttttgtaaagataattctgatgaaaaacaataatttcgttttgacgttgcgaaagcggctcttttacaacgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaatgcaaaacaataggtttt.......................................... Subject: pint_PI0051_PI0054|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[53449,54718]|1270 HSP 1 e-value: 2.0E-45 bit: 184.0 Len: 145 Query Start:215 Query End:359 Subject Strand: NEGATIVE Subject Start: 74 Subject End: 218 ......................................................................................................................................................................................................................ttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ ......................................................................................................................................................................................................................ttttgtaaagattatttctttagaaaatggtaatttcgatttgacattgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttgcaacacgttgata............................ Subject: pint_PI0770_PI0771|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[706415,706663]|249 HSP 1 e-value: 2.0E-44 bit: 180.0 Len: 147 Query Start:213 Query End:359 Subject Strand: NEGATIVE Subject Start: 97 Subject End: 243 ....................................................................................................................................................................................................................ggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ ....................................................................................................................................................................................................................ggttttgtaaaaataattccgttaaaaagcgataactgcgctttgacgttgcgaaagcggctcttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttataacacattgata............................ Subject: pint_PI2051_PI2052|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2007896,2008733]|838 HSP 1 e-value: 4.0E-43 bit: 176.0 Len: 163 Query Start:183 Query End:345 Subject Strand: NEGATIVE Subject Start: 332 Subject End: 492 ......................................................................................................................................................................................ctttcagccaacacgtgggaaaatcgcctcggttttgtaaagataatttcgctga-aaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... ......................................................................................................................................................................................ctttaagccaacacgtg--aaaatcgctttggttttgtaaagataatt-cgttaagaaaacgataatttcgatttgacgttgcgaaagcagctcttttgcaacgcaaaacctatgcttttaccgtgcaaaacagccgcttttaggatgcaaaacaataggtttt.......................................... Subject: pint_PI1485_PI1486|hypothetical protein:alginate O-acetylation protein|POSITIVE:POSITIVE|[1405400,1405646]|247 HSP 1 e-value: 1.0E-42 bit: 174.0 Len: 140 Query Start:214 Query End:353 Subject Strand: NEGATIVE Subject Start: 90 Subject End: 229 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacg.................................. .....................................................................................................................................................................................................................gttttgtaaagataattctgttaaaaactgataactgcgttttgacattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaacgcaaaacaataggttttgcaatacg.................................. Subject: pint_PI1583_PI1584|phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR)/IMP cyclohydrolase (ATIC):A/G-specific adenine glycosylase|POSITIVE:POSITIVE|[1511261,1512037]|777 HSP 1 e-value: 6.0E-42 bit: 172.0 Len: 147 Query Start:213 Query End:359 Subject Strand: NEGATIVE Subject Start: 397 Subject End: 543 ....................................................................................................................................................................................................................ggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ ....................................................................................................................................................................................................................ggttttgtaaagataattacattgaaaattgataatttcgttttgacgttgcgaaagcggctgttttgcgaggtaaaacctacgcttttgccgtgcaaaacagccgcttttggaatgcaaaacaataggttttgtaacacgttgata............................ Subject: pint_PI1391_PI1392|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1311755,1312040]|286 HSP 1 e-value: 4.0E-40 bit: 167.0 Len: 132 Query Start:214 Query End:345 Subject Strand: NEGATIVE Subject Start: 57 Subject End: 188 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... .....................................................................................................................................................................................................................gttttgtaaagataattccgacgaaaaacggtaatttcgatttggcgttgcgaaagcggctgttttgcagcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt.......................................... Subject: pint_PI1577_PI1578|conserved hypothetical protein:rod shape-determining protein|POSITIVE:POSITIVE|[1503940,1504327]|388 HSP 1 e-value: 2.0E-38 bit: 161.0 Len: 109 Query Start:238 Query End:346 Subject Strand: POSITIVE Subject Start: 92 Subject End: 200 .............................................................................................................................................................................................................................................aaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... .............................................................................................................................................................................................................................................aaaactataatttcgatttgacgttgcgaaagcggctcttttgcgctgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... Subject: pint_PI0990_PI0991|GDP-mannose 4,6-dehydratase:conserved hypothetical protein|POSITIVE:POSITIVE|[915115,915448]|334 HSP 1 e-value: 2.0E-38 bit: 161.0 Len: 129 Query Start:217 Query End:345 Subject Strand: POSITIVE Subject Start: 170 Subject End: 298 ........................................................................................................................................................................................................................ttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... ........................................................................................................................................................................................................................ttgtaaagataattccgttaaaaagtgataactgcgctttggcattgcgaaagcggctcttttgcattgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggtttt.......................................... Subject: pint_PI2178_PI0001|1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, GcpE:possible Ton-B receptor|POSITIVE:POSITIVE|[1,539]|539 HSP 1 e-value: 9.0E-38 bit: 159.0 Len: 144 Query Start:215 Query End:358 Subject Strand: POSITIVE Subject Start: 111 Subject End: 254 ......................................................................................................................................................................................................................ttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgat............................. ......................................................................................................................................................................................................................ttttgtaaagataattctgttaaaaactgataactgcgctttggcgttgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggttttataatgcgttgat............................. Subject: pint_PI0780_PI0781|DNA gyrase, subunit A (DNA topoisomerase (ATP-hydrolyzing)):UDP-glucose/GDP-mannose dehydrogenase family; probable UDP-glucose 6-dehydrogenase|POSITIVE:POSITIVE|[718574,719399]|826 HSP 1 e-value: 9.0E-38 bit: 159.0 Len: 144 Query Start:216 Query End:359 Subject Strand: POSITIVE Subject Start: 480 Subject End: 623 .......................................................................................................................................................................................................................tttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ .......................................................................................................................................................................................................................tttgtaaagataattctgttaaaaagtgataactgcgctttggcattgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccatgcaaaacagccgcttttggaatgcaaaacaataggttttgtaatgcgttgata............................ Subject: pint_PI0412_PI0413|primosomal protein N', replication factor Y:outer membrane protein|POSITIVE:POSITIVE|[382213,382552]|340 HSP 1 e-value: 1.0E-36 bit: 155.0 Len: 146 Query Start:214 Query End:359 Subject Strand: NEGATIVE Subject Start: 83 Subject End: 228 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ .....................................................................................................................................................................................................................gttttgtaaagataattctgttaaaaactgataactgcgctttggcattgcgaaagcggctcttttgcgctgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggttttgcaatgcattgata............................ Subject: pint_PI0010_PI0011|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[11740,12281]|542 HSP 1 e-value: 5.0E-36 bit: 153.0 Len: 145 Query Start:214 Query End:358 Subject Strand: POSITIVE Subject Start: 49 Subject End: 193 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgat............................. .....................................................................................................................................................................................................................gttttgtaaagataattccgttaaaaagtgataactgcgctttgacgttgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggttttgtaatacgctgat............................. Subject: pint_PI2056_PI2057|conserved hypothetical protein:conserved hypothetical protein; possible metal-dependent membrane protease|POSITIVE:POSITIVE|[2014489,2015078]|590 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 143 Query Start:203 Query End:345 Subject Strand: POSITIVE Subject Start: 329 Subject End: 471 ..........................................................................................................................................................................................................aaatcgcctcggttttgtaaagataatttcgctga-aaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... ..........................................................................................................................................................................................................aaatcgccttgattttgtaaagataatt-cgttaagaaaacaataatttcgatttgacgttgcgaaagcggctcttttgtaatgcaaaacttacgcttttaccatgcaaaacagccgcttttggaatgcgaaacaataggtttt.......................................... Subject: pint_PI1503_PI1504|30S ribosomal protein S15:non-specific DNA-binding protein|POSITIVE:POSITIVE|[1423841,1424457]|617 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 132 Query Start:214 Query End:345 Subject Strand: POSITIVE Subject Start: 145 Subject End: 276 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... .....................................................................................................................................................................................................................gttttgtaaagataattctcttaaaaaatgataactgcgttttgacattgcgaaagcggctgttttgcgttgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggtttt.......................................... Subject: pint_PI1424_PI1425|probable peptidase family M48:conserved hypothetical protein|POSITIVE:POSITIVE|[1344105,1344627]|523 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 124 Query Start:236 Query End:359 Subject Strand: NEGATIVE Subject Start: 93 Subject End: 216 ...........................................................................................................................................................................................................................................gaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ ...........................................................................................................................................................................................................................................gaaaaatgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacagccgcttttgccgtgtaaaacctacgcttttggaatgcaaaacaataggttttgcaatgtgttgata............................ Subject: pint_PI1029_PI1030|seryl-tRNA synthetase:biotin carboxyl carrier protein|POSITIVE:POSITIVE|[947266,947985]|720 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 144 Query Start:216 Query End:359 Subject Strand: POSITIVE Subject Start: 310 Subject End: 453 .......................................................................................................................................................................................................................tttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ .......................................................................................................................................................................................................................tttgtaaagataatttctttaaaaagtgataactgcgctttggcgttgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggtcttgtaatgcgttgata............................ Subject: pint_PI0680_PI0682|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[618694,619111]|418 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 132 Query Start:214 Query End:345 Subject Strand: POSITIVE Subject Start: 113 Subject End: 244 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... .....................................................................................................................................................................................................................gttttgtaaagattattctgttaaaaagcgataactgcgctttgacattgcgaaagcggctcttttgcgatgcaaaacctacgcttttagcgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... Subject: pint_PI0145_PI0146|secDF export membrane protein:transcription elongation factor|POSITIVE:POSITIVE|[139785,140477]|693 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 140 Query Start:213 Query End:352 Subject Strand: NEGATIVE Subject Start: 226 Subject End: 364 ....................................................................................................................................................................................................................ggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatac................................... ....................................................................................................................................................................................................................ggttttgtaaagataatt-cattgaaaaacgataatttcgatttggcattgcgaaagcgtaggttttgcaatgcaaaacagccgcttttaccgtgcaaaacctacacttttggaatgcaaaacaataggttttataatac................................... HSP 2 e-value: 4.0E-6 bit: 54.0 Len: 35 Query Start:266 Query End:300 Subject Strand: POSITIVE Subject Start: 278 Subject End: 312 .........................................................................................................................................................................................................................................................................aaagcggctgttttgcaacgcaaaacctacgcttt....................................................................................... .........................................................................................................................................................................................................................................................................aaagcggctgttttgcattgcaaaacctacgcttt....................................................................................... Subject: pint_PI1915_PI1916|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1859967,1860350]|384 HSP 1 e-value: 8.0E-35 bit: 149.0 Len: 146 Query Start:201 Query End:346 Subject Strand: POSITIVE Subject Start: 100 Subject End: 245 ........................................................................................................................................................................................................gaaaatcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaa-cctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... ........................................................................................................................................................................................................gaaaatcgccttgattttgtaaaaataattctgacgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcacggtaaaagcgtatg-ttttacgttgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... HSP 2 e-value: 3.0E-19 bit: 97.6 Len: 57 Query Start:266 Query End:322 Subject Strand: NEGATIVE Subject Start: 165 Subject End: 221 .........................................................................................................................................................................................................................................................................aaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttt................................................................. .........................................................................................................................................................................................................................................................................aaagcggctgttttgcaacgtaaaacatacgcttttaccgtgcaaaacagccgcttt................................................................. Subject: pint_PI1494_PI1495|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1414029,1414571]|543 HSP 1 e-value: 8.0E-35 bit: 149.0 Len: 131 Query Start:216 Query End:346 Subject Strand: POSITIVE Subject Start: 200 Subject End: 330 .......................................................................................................................................................................................................................tttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... .......................................................................................................................................................................................................................tttgtaaagataattccgttaaaaagtgataactgcgctttgacattgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgtttttggaacgcaaaacaataggtttta......................................... Subject: pint_PI1499_PI1500|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1420241,1420765]|525 HSP 1 e-value: 3.0E-34 bit: 147.0 Len: 130 Query Start:216 Query End:345 Subject Strand: POSITIVE Subject Start: 149 Subject End: 278 .......................................................................................................................................................................................................................tttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... .......................................................................................................................................................................................................................tttgtaaagataattccgttaaaaagtgataaccgcgctttgacattgcgaaagcggctcttttgcgatgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggtttt.......................................... Subject: pint_PI0945_PI0946|sodium:solute symporter family protein:glutathione peroxidase|POSITIVE:POSITIVE|[877685,878112]|428 HSP 1 e-value: 3.0E-34 bit: 147.0 Len: 158 Query Start:215 Query End:372 Subject Strand: POSITIVE Subject Start: 79 Subject End: 236 ......................................................................................................................................................................................................................ttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttat............... ......................................................................................................................................................................................................................ttttgtaaagataatttcgttgaaaagtggtaattgcgctttggcattgcgaaagcggctgttttgcgatgcaaaagagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggttttgtaatgtgttgatacacagattgttat............... Subject: pint_PI0854_PI0855|MiaB-like tRNA modifying enzyme:hypothetical protein|POSITIVE:POSITIVE|[788060,789233]|1174 HSP 1 e-value: 3.0E-34 bit: 147.0 Len: 130 Query Start:236 Query End:365 Subject Strand: POSITIVE Subject Start: 762 Subject End: 891 ...........................................................................................................................................................................................................................................gaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacaga...................... ...........................................................................................................................................................................................................................................gaaaaatgataatttcgatttggcgttgcgaaagcgtaggttttgcaacgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttataatatgttgataaacaga...................... Subject: pint_PI0141_PI0142|glutamine amidotransferase involved in pyridoxine biosynthesis:DNA-binding protein|POSITIVE:POSITIVE|[133369,133978]|610 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 132 Query Start:214 Query End:345 Subject Strand: POSITIVE Subject Start: 165 Subject End: 296 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaac-gataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... .....................................................................................................................................................................................................................gttttgtaaagataatt-ctcttaaaaattgataactgcgttttgacattgcgaaagcggctgttttgcgctgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaacgcaaaacaataggtttt.......................................... Subject: pint_PI0195_PI0196|epimerase/reductase:hypothetical protein|POSITIVE:POSITIVE|[188263,188574]|312 HSP 1 e-value: 5.0E-33 bit: 143.0 Len: 100 Query Start:246 Query End:345 Subject Strand: POSITIVE Subject Start: 176 Subject End: 275 .....................................................................................................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... .....................................................................................................................................................................................................................................................aatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt.......................................... Subject: pint_PI2172_PI2174|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2114052,2114384]|333 HSP 1 e-value: 1.0E-30 bit: 135.0 Len: 144 Query Start:215 Query End:358 Subject Strand: NEGATIVE Subject Start: 82 Subject End: 225 ......................................................................................................................................................................................................................ttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgat............................. ......................................................................................................................................................................................................................ttttgtaaagataattctgttaaaaactgataactgcgctttgacgttgcgaaagcggctcttttacgatgcaaaacctacgcttttaccgtgcaaaagagccgcttttggaacgcaaaacaataggttttgtaatacgttgat............................. Subject: pint_PI0948_PI0949|hypothetical protein:endonuclease|POSITIVE:POSITIVE|[882039, 882403]|365 HSP 1 e-value: 2.0E-29 bit: 131.0 Len: 142 Query Start:238 Query End:379 Subject Strand: POSITIVE Subject Start: 164 Subject End: 305 .............................................................................................................................................................................................................................................aaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatag........ .............................................................................................................................................................................................................................................aaaatgataatttcgatttggcattgcgaaagcggctgttttgcatcgcaaaacagccgctttgaccgtgtaaaacctacgcttttacaatgcaaaacaataggttttgcattgcgttgataatgagaatgttatacaatag........ Subject: pint_PI1117_PI1118|glycosyl transferase, family 2:glycosyl transferases, group 1|POSITIVE:POSITIVE|[1042340,1042635]|296 HSP 1 e-value: 5.0E-27 bit: 123.0 Len: 110 Query Start:241 Query End:350 Subject Strand: NEGATIVE Subject Start: 84 Subject End: 193 ................................................................................................................................................................................................................................................acgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaat..................................... ................................................................................................................................................................................................................................................acgataaattcactttgacattccaaaagcggttgttttgcgatgcaaaacctacgcttttaccgtgtaaaacagccgcttttggaacgcgaaacaataggttttacaat..................................... Subject: pint_PI1044_PI1045|excinuclease ABC, subunit A:cytidine/deoxycytidylate deaminase family protein|POSITIVE:POSITIVE|[964700,965243]|544 HSP 1 e-value: 8.0E-26 bit: 119.0 Len: 128 Query Start:254 Query End:381 Subject Strand: POSITIVE Subject Start: 226 Subject End: 353 .............................................................................................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgttatacaatagtt...... .............................................................................................................................................................................................................................................................tttgacattgcgaaagtggctgttttgcaacgcaaaacagccgcttttatcgtgtaaaacctacgcttttggaatgcaaaacaataggttttgtaatacattgatgtatagatagttatgcaatagtt...... Subject: pint_PI1981_PI1982|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1938199,1938578]|380 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 138 Query Start:214 Query End:351 Subject Strand: POSITIVE Subject Start: 143 Subject End: 280 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaata.................................... .....................................................................................................................................................................................................................gttttgtaaagataattctgataaaaaacagcaatttcggtttgacattgcgaaagcggctgttttgcatttcaaaacagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggttttataata.................................... Subject: pint_PI1958_PI1959|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1917430,1917847]|418 HSP 1 e-value: 5.0E-24 bit: 113.0 Len: 137 Query Start:215 Query End:351 Subject Strand: POSITIVE Subject Start: 182 Subject End: 318 ......................................................................................................................................................................................................................ttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaata.................................... ......................................................................................................................................................................................................................ttttgtaaagataattctgataaaaaacagcaatttcggtttgacattgcaaaagcggctgttttgcattgcaaaacagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaataggttttataata.................................... Subject: pint_PI0962_PI0963|phosphoglycerate mutase:hypothetical protein|POSITIVE:POSITIVE|[892763,893337]|575 HSP 1 e-value: 1.0E-21 bit: 105.0 Len: 132 Query Start:214 Query End:345 Subject Strand: POSITIVE Subject Start: 143 Subject End: 274 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaac-gataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... .....................................................................................................................................................................................................................gttttgtaaagataatt-cgttgaaaaaatgataatttcgatttgacattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt.......................................... HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 35 Query Start:266 Query End:300 Subject Strand: NEGATIVE Subject Start: 195 Subject End: 229 .........................................................................................................................................................................................................................................................................aaagcggctgttttgcaacgcaaaacctacgcttt....................................................................................... .........................................................................................................................................................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt....................................................................................... Subject: pint_PI0831_PI0832|hypothetical protein:sigma-54-dependent transcriptional regulator|POSITIVE:POSITIVE|[768509,768973]|465 HSP 1 e-value: 7.0E-20 bit: 99.6 Len: 154 Query Start:206 Query End:359 Subject Strand: NEGATIVE Subject Start: 165 Subject End: 318 .............................................................................................................................................................................................................tcgcctcggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ .............................................................................................................................................................................................................tcgcttcggctttgtaaagataattccgttaaaaagtgacaactgcgctttgacgttgcgaaagcgtaggttttgcaatgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaacgcaaaacaataggttttacaaagcgttgata............................ HSP 2 e-value: 4.0E-6 bit: 54.0 Len: 35 Query Start:266 Query End:300 Subject Strand: POSITIVE Subject Start: 224 Subject End: 258 .........................................................................................................................................................................................................................................................................aaagcggctgttttgcaacgcaaaacctacgcttt....................................................................................... .........................................................................................................................................................................................................................................................................aaagcggctgttttgcattgcaaaacctacgcttt....................................................................................... Subject: pint_PI0210_PI0211|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[203859,204206]|348 HSP 1 e-value: 7.0E-20 bit: 99.6 Len: 133 Query Start:213 Query End:345 Subject Strand: POSITIVE Subject Start: 199 Subject End: 330 ....................................................................................................................................................................................................................ggttttgtaaagataatttcgctgaaaaac-gataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... ....................................................................................................................................................................................................................ggttttgtaaagataatt-ctcttaaaaagtgataactgcgctttggcgttgcgaaagcggctgttt-gcgatgcaaaacagccgcttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggtttt.......................................... Subject: pint_PI1909_PI1910|DNA gyrase, B subunit:hypothetical protein|POSITIVE:POSITIVE|[1855331,1855917]|587 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 113 Query Start:254 Query End:366 Subject Strand: NEGATIVE Subject Start: 326 Subject End: 438 .............................................................................................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagat..................... .............................................................................................................................................................................................................................................................tttgacattgcgaaagcgtaggttttgcgatgcaaaagcgccacttttaccgtgcaaaacctacgcttttggaatgcaaaacaataggttttgcaatgcgttgataaacagat..................... Subject: pint_PI1441_PI1442|hypothetical protein:radical-activating enzyme (pyruvate-formate lyase-activating enzyme)|POSITIVE:POSITIVE|[1363734,1364088]|355 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 133 Query Start:213 Query End:345 Subject Strand: NEGATIVE Subject Start: 86 Subject End: 218 ....................................................................................................................................................................................................................ggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... ....................................................................................................................................................................................................................ggttttgtaaagataatttacaaggaaaacggtattttcgatttgacgttgcgaaagcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaacgcaaaacaataggtttt.......................................... Subject: pint_PI1688_PI1689|possible GTPase:conserved hypothetical protein|POSITIVE:POSITIVE|[1603964,1604449]|486 HSP 1 e-value: 1.0E-18 bit: 95.6 Len: 132 Query Start:215 Query End:346 Subject Strand: NEGATIVE Subject Start: 173 Subject End: 304 ......................................................................................................................................................................................................................ttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... ......................................................................................................................................................................................................................ttttgtaaagataattcggagggaaaatgataatttcgatttgacgttgcgaaagcgtatgttttgcgatgcaaaagagccgcttttaccgtgcaaaacctacgcttttacaatgcaaaacaataggtttta......................................... Subject: pint_PI0652_PI0653|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[595853,596185]|333 HSP 1 e-value: 6.0E-14 bit: 79.8 Len: 132 Query Start:214 Query End:345 Subject Strand: POSITIVE Subject Start: 146 Subject End: 277 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... .....................................................................................................................................................................................................................gttttgtaaagataattctgaagaaaaacgataatttcgatttggcactgcgaaagtgtaggttttaccgtccaaaagcggctcttttgccttgcaaaacctacgcttttggaatgcaaaacaataggtttt.......................................... Subject: pint_PI1360_PI1361|conserved hypothetical protein:CTP synthase (UTP-ammonia lyase)|POSITIVE:POSITIVE|[1277656,1278384]|729 HSP 1 e-value: 3.0E-13 bit: 77.8 Len: 131 Query Start:215 Query End:345 Subject Strand: POSITIVE Subject Start: 191 Subject End: 321 ......................................................................................................................................................................................................................ttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttt.......................................... ......................................................................................................................................................................................................................ttttgtaaagataatttcgttaaaaagtgataactgcactttgacgttgcgaaagcggctgttttacgatgcaaaacagccgcttttaccgtgcaaaacctacgcttttaggacgcaaaacaatagatttt.......................................... Subject: pint_PI1728_PI1729|ATP-dependent Clp protease, proteolytic subunit:FKBP-type peptidyl-prolyl cis-trans isomerase, trigger factor|POSITIVE:POSITIVE|[1647719,1648555]|837 HSP 1 e-value: 1.0E-12 bit: 75.8 Len: 133 Query Start:214 Query End:346 Subject Strand: NEGATIVE Subject Start: 415 Subject End: 547 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctga-aaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggtttta......................................... .....................................................................................................................................................................................................................gttttgtaaagataatt-cgttgagaaaacggtaatttcgatttgacgttgcaaaagcgtaggttttgcaaggcaaaagagccgcttttaccgtgcaaaagcggctcttttacaatgcgaaacaataggtttta......................................... Subject: pint_PI0932_PI0933|conserved hypothetical protein; possible hemin receptor:hypothetical protein|POSITIVE:POSITIVE|[865425,865839]|415 HSP 1 e-value: 1.0E-12 bit: 75.8 Len: 138 Query Start:213 Query End:350 Subject Strand: NEGATIVE Subject Start: 174 Subject End: 311 ....................................................................................................................................................................................................................ggttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaat..................................... ....................................................................................................................................................................................................................ggttttgtaaagataattttattaaaaagtggcaattgcgttttgacattgcaaaagcgtaggttttgcaatgcaaaagagccgcttttaccgtgcaaaacctacgcttttggaacgcgaaacaataggttttacaat..................................... Subject: pint_PI0874_PI0875|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[810342,810747]|406 HSP 1 e-value: 1.0E-12 bit: 75.8 Len: 158 Query Start:214 Query End:371 Subject Strand: NEGATIVE Subject Start: 165 Subject End: 322 .....................................................................................................................................................................................................................gttttgtaaagataatttcgctgaaaaacgataatttcgatttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgatatacagattgtta................ .....................................................................................................................................................................................................................gttttgtaaagataattcccttaaaaaaggataactgcgctttggcattgcgaaagcgtaggttttgcgttgcaaaagagccgcttttaccgtgtaaaacctacgcttttggaatgcaaaacaacacgttttataacacgctgatgtatagatagtta................ HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 35 Query Start:266 Query End:300 Subject Strand: POSITIVE Subject Start: 236 Subject End: 270 .........................................................................................................................................................................................................................................................................aaagcggctgttttgcaacgcaaaacctacgcttt....................................................................................... .........................................................................................................................................................................................................................................................................aaagcggctcttttgcaacgcaaaacctacgcttt....................................................................................... Subject: pint_PI1324_PI1325|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1243086,1243554]|469 HSP 1 e-value: 2.0E-10 bit: 67.9 Len: 106 Query Start:254 Query End:359 Subject Strand: NEGATIVE Subject Start: 248 Subject End: 353 .............................................................................................................................................................................................................................................................tttggcattgcgaaagcggctgttttgcaacgcaaaacctacgcttttaccgtgcaaaacagccgcttttggaatgcaaaacaataggttttacaatacgttgata............................ .............................................................................................................................................................................................................................................................tttggcattgtaaaagcggctgttttgcatcgcaaaacagccgcttttggctggtaaaagagccgcttttacaatgtaaaacaataggttttgtaattcgttgata............................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACAGCGUACAGAACAUAAAAAACGUGUAGUUCCGAAUGGCAUUGUGCCUAUCAGGACUACACGUUUUUUUAUGCUUUUAUGCCGCUCAGUGUAGGUAGGUGCGUGGUUUAUUGCGUUUCCUGGCACUGAAUAAGAAAGGGGUUAUAUAGUAAUUCUAGUUUAUCUAGGAUUUCUAGAAACUUUCAGCCAACACGUGGGAAAAUCGCCUCGGUUUUGUAAAGAUAAUUUCGCUGAAAAACGAUAAUUUCGAUUUGGCAUUGCGAAAGCGGCUGUUUUGCAACGCAAAACCUACGCUUUUACCGUGCAAAACAGCCGCUUUUGGAAUGCAAAACAAUAGGUUUUACAAUACGUUGAUAUACAGAUUGUUAUACAAUAGUUAUACUU ....(((((((((((((..((((((((((((((((.((..(((.....)))..)).))))))))))))))))...((..((.(.((((((((((((((.(..((((........))))..)))).)))))))....(((((...((...(((.((((((((.....)))))))).))).)).))))).........)))).).))..))....))))))))..........))))).....(((.....))).....(((((.(((((((((((((((((((.((.((((.......))))..))))))))))))))))))))).)))))..((((((..((....(((....)))....))..))))))................. (-127.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUAUAACUAUUGUAUAACAAUCUGUAUAUCAACGUAUUGUAAAACCUAUUGUUUUGCAUUCCAAAAGCGGCUGUUUUGCACGGUAAAAGCGUAGGUUUUGCGUUGCAAAACAGCCGCUUUCGCAAUGCCAAAUCGAAAUUAUCGUUUUUCAGCGAAAUUAUCUUUACAAAACCGAGGCGAUUUUCCCACGUGUUGGCUGAAAGUUUCUAGAAAUCCUAGAUAAACUAGAAUUACUAUAUAACCCCUUUCUUAUUCAGUGCCAGGAAACGCAAUAAACCACGCACCUACCUACACUGAGCGGCAUAAAAGCAUAAAAAAACGUGUAGUCCUGAUAGGCACAAUGCCAUUCGGAACUACACGUUUUUUAUGUUCUGUACGCUGUUUA ..(((((...((((.....))))...)))))...(((.(((((((.....((((((.(((((.(.(((((((((((((((((((...(((((....))))).)).))))))))))))))))).).))))).))).))).....((((......)))).......))))))).......(((..(((((...(..((((..(((((..(((((.....))))).....(((.....)))........)))))....))))..)..))))))))........))).............((((((((.((..(((((((((((.((((((((.((((..(((.....)))..)))).)))))))))))))))))))...)).)))))))) (-112.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 979 320 271 74 25 pint:PI2153|5end_hypothetical_protein_2099392..2099622_POSITIVE 979 320 271 200 151 pint:PI0788|5end_3-methyladenine_DNA_glycosylase_(DNA-3-methyladenine_glycosylase_I)_726152..726382_POSITIVE 960 310 261 64 15 pint:PI1567|5end_hypothetical_protein_1489229..1489459_POSITIVE 960 310 261 206 157 pint:PI1486|5end_alginate_O-acetylation_protein_1405507..1405737_POSITIVE 948 330 281 215 166 pint:PI0771|5end_conserved_hypothetical_protein_706524..706754_POSITIVE 907 340 291 51 2 pint:PI1905|5end_hypothetical_protein_1847628..1847858_POSITIVE 907 340 291 94 45 pint:PI1346|5end_hypothetical_protein_1260814..1261044_POSITIVE 907 340 291 55 6 pint:PI1339|5end_hypothetical_protein_1256671..1256901_POSITIVE 907 340 291 82 33 pint:PI1129|5end_hypothetical_protein_1055377..1055607_POSITIVE 907 340 291 55 6 pint:PI0104|5end_hypothetical_protein_99105..99335_POSITIVE 907 340 291 66 17 pint:PI0061|5end_hypothetical_protein_60500..60730_POSITIVE 907 340 291 143 94 pint:PI0055|5end_hypothetical_protein_54815..55045_POSITIVE 907 340 291 55 6 pint:PI0053|5end_hypothetical_protein_54727..54957_POSITIVE 907 340 291 66 17 pint:PI0004|5end_hypothetical_protein_6199..6429_POSITIVE 881 340 291 94 45 pint:PI2002|5end_hypothetical_protein_1960662..1960892_POSITIVE 856 340 291 94 45 pint:PI1076|5end_hypothetical_protein_998893..999123_POSITIVE 855 320 271 74 25 pint:PI1829|5end_hypothetical_protein_1769255..1769485_POSITIVE 855 340 291 55 6 pint:PI0099|5end_hypothetical_protein_95530..95760_POSITIVE 851 310 261 64 15 pint:PI1934|5end_hypothetical_protein_1882363..1882593_POSITIVE 851 310 261 72 23 pint:PI1168|5end_hypothetical_protein_1089696..1089926_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 907 340 291 136 87 pint:PI1141|3end_hypothetical_protein_1069713..1069863_POSITIVE 907 340 291 77 28 pint:PI0054|3end_hypothetical_protein_54829..54979_POSITIVE 895 290 241 147 98 pint:PI1905|3end_hypothetical_protein_1847854..1848004_POSITIVE 888 310 261 146 97 pint:PI1814|3end_hypothetical_protein_1753233..1753383_POSITIVE 879 220 171 132 83 pint:PI2153|3end_hypothetical_protein_2099630..2099780_POSITIVE 878 340 291 136 87 pint:PI0759|3end_hypothetical_protein_693998..694148_POSITIVE 874 340 292 49 1 pint:PI0688|3end_hypothetical_protein_625750..625900_POSITIVE 874 340 292 49 1 pint:PI0186|3end_hypothetical_protein_178003..178153_POSITIVE 860 310 261 120 71 pint:PI1356|3end_hypothetical_protein_1273340..1273490_POSITIVE 851 310 261 150 101 pint:PI1922|3end_hypothetical_protein_1865454..1865604_POSITIVE 851 310 261 146 97 pint:PI1340|3end_hypothetical_protein_1256872..1257022_POSITIVE 830 340 291 77 28 pint:PI0996|3end_hypothetical_protein_920660..920810_POSITIVE 812 310 261 146 97 pint:PI0689|3end_hypothetical_protein_625877..626027_POSITIVE 780 349 301 52 4 pint:PI0669|3end_hypothetical_protein_611147..611297_POSITIVE 779 310 261 146 97 pint:PI0061|3end_hypothetical_protein_60690..60840_POSITIVE 777 300 254 150 104 pint:PI1528|3end_hypothetical_protein_1449878..1450028_POSITIVE 770 310 261 146 97 pint:PI0004|3end_hypothetical_protein_6389..6539_POSITIVE 739 310 261 146 97 pint:PI0158|3end_hypothetical_protein_151298..151448_POSITIVE 736 290 243 120 73 pint:PI0182|3end_hypothetical_protein_174277..174427_POSITIVE 731 359 312 48 1 pint:PI1813|3end_hypothetical_protein_1753086..1753236_POSITIVE