Origin IGS: ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttctaatcttttcatacagttttacctttttattcgatacttagaccgttctcaataatttgttgtaaaagtagtatttttttctgattgtcgtttcctttgctgctttaataattcttaatgggctgcgttttcagaaatccggcaaagtataataaaaataactaaaaaaccatctctattccgtaattttgcagtacttttgtaatttaaaataattggataagaa Mask Tandem Repeat Region ================================================ ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Find is-nt database================================================ Query_seq: PI0013:PI0014|PI0013:PI0014:auxin-regulated protein:hypothetical protein:->->:17167..17395 229 ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: PI0013:PI0014|PI0013:PI0014:auxin-regulated protein:hypothetical protein:->->:17167..17395 229 ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: PI0013:PI0014|PI0013:PI0014:auxin-regulated protein:hypothetical protein:->->:17167..17395 229 ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 11 End: 109 .......... M I L N Y K S T A K L R N R D G F L V I F I I L C R I S E N A A H ........................................................................................................................ ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: PI0013:PI0014|PI0013:PI0014:auxin-regulated protein:hypothetical protein:->->:17167..17395 229 ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Intra-Species Hit: Count: 1 Min: 1 Max: 229 Len: 229 Subject: pint_PI0013_PI0014|auxin-regulated protein:hypothetical protein|POSITIVE:POSITIVE|[17167,17395]|229 HSP 1 e-value: 1.0E-114 bit: 412.0 Len: 229 Query Start:1 Query End:229 Subject Strand: POSITIVE Subject Start: 1 Subject End: 229 ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagnnnnnnntactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa ttcttatccaattattttaaattacaaaagtactgcaaaattacggaatagagatggttttttagttatttttattatactttgccggatttctgaaaacgcagcccattaagaattattaaagcagcaaaggaaacgacaatcagaaaaaaatactacttttacaacaaattattgagaacggtctaagtatcgaataaaaaggtaaaactgtatgaaaagattagaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUUAUCCAAUUAUUUUAAAUUACAAAAGUACUGCAAAAUUACGGAAUAGAGAUGGUUUUUUAGUUAUUUUUAUUAUACUUUGCCGGAUUUCUGAAAACGCAGCCCAUUAAGAAUUAUUAAAGCAGCAAAGGAAACGACAAUCAGAAAAAAAUACUACUUUUACAACAAAUUAUUGAGAACGGUCUAAGUAUCGAAUAAAAAGGUAAAACUGUAUGAAAAGAUUAGAA ......((.((((.(((((...((((....((((.....(((.((.(((((((((..((....))..))))))))).(((((.((((..(((((((....((.((...((((......)))).)).))...(....).....)))))))..................(((....)))....))))..))))).))))).....))))....))))))))).)))).)). (-35.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUAAUCUUUUCAUACAGUUUUACCUUUUUAUUCGAUACUUAGACCGUUCUCAAUAAUUUGUUGUAAAAGUAGUAUUUUUUUCUGAUUGUCGUUUCCUUUGCUGCUUUAAUAAUUCUUAAUGGGCUGCGUUUUCAGAAAUCCGGCAAAGUAUAAUAAAAAUAACUAAAAAACCAUCUCUAUUCCGUAAUUUUGCAGUACUUUUGUAAUUUAAAAUAAUUGGAUAAGAA .................((((.....((((((((..((((((.(.(((..((((.(((...(((((.(((((((((........((.....)).......))))))))).)))))..))).))))(((......))).....)))).)))))))))))))).))))...........(((..((((..((((((...(((....)))...))))))...))))..))). (-34.36) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 300 149 101 230 175 pint:PI1419|5end_conserved_hypothetical_protein;_membrane_protease_subunits,_stomatin/prohibitin_homologs_1339136..1339366_POSITIVE 287 139 99 127 90 pint:PI1476|5end_acyl-CoA_synthase/O-succinylbenzoic_acid-CoA_ligase_1397699..1397929_POSITIVE 267 125 83 97 50 pint:PI1763|5end_two-component_system_sensor_histidine_kinase/response_regulator_1685877..1686107_POSITIVE 262 106 80 199 175 pint:PI1000|5end_conserved_hypothetical_protein_923332..923562_POSITIVE 245 128 81 88 33 pint:PI0207|5end_conserved_hypothetical_protein_199797..200027_POSITIVE 243 115 76 83 41 pint:PI0171|5end_hypothetical_protein_163850..164080_POSITIVE 236 139 101 198 152 pint:PI1753|5end_conserved_hypothetical_protein_1680291..1680521_POSITIVE 233 140 93 55 13 pint:PI0723|5end_conserved_hypothetical_protein_656043..656273_POSITIVE 232 148 101 91 25 pint:PI1887|5end_cell_division_protein_1829115..1829345_POSITIVE 232 106 86 26 4 pint:PI1801|5end_30S_ribosomal_protein_S9_1733949..1734179_POSITIVE 229 129 81 136 79 pint:PI0688|5end_hypothetical_protein_625458..625688_POSITIVE 228 130 82 47 1 pint:PI0666|5end_chorismate_synthase_609006..609236_POSITIVE 227 106 69 147 101 pint:PI1501|5end_GTP-binding_elongation_factor_family_protein_TypA/BipA_1421424..1421654_POSITIVE 223 148 101 63 26 pint:PI1729|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase,_trigger_factor_1648416..1648646_POSITIVE 223 89 44 127 95 pint:PI1009|5end_cytochrome_c_nitrite_reductase,_large_subunit_929342..929572_POSITIVE 222 186 143 171 122 pint:PI1192|5end_carboxynorspermidine_decarboxylase_1111113..1111343_POSITIVE 222 117 82 56 12 pint:PI0806|5end_heavy_metal_efflux_pump_743679..743909_POSITIVE 219 75 32 97 48 pint:PI0492|5end_hypothetical_protein_451701..451931_POSITIVE 218 140 91 77 33 pint:PI0843|5end_carboxyl-terminal_protease_776145..776375_POSITIVE 218 142 104 212 166 pint:PI0030|5end_transcriptional_regulator,_AsnC_family_34509..34739_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 106 80 123 99 pint:PI0998|3end_possible_flavin_reductase_domain_protein_923336..923486_POSITIVE 255 105 65 119 79 pint:PI0572|3end_tRNA_(5-methylaminomethyl-2-thiouridylate)-methyltransferase_517589..517739_POSITIVE 246 163 122 111 74 pint:PI0792|3end_conserved_hypothetical_protein_729627..729777_POSITIVE 245 128 81 67 12 pint:PI0206|3end_TPR-repeat-containing_protein_199856..200006_POSITIVE 244 159 127 134 101 pint:PI1587|3end_conserved_hypothetical_protein;_possible_permease_1516266..1516416_POSITIVE 243 115 76 44 2 pint:PI0170|3end_hypothetical_protein_163891..164041_POSITIVE 241 88 41 98 45 pint:PI1634|3end_conserved_hypothetical_protein_1553819..1553969_POSITIVE 236 139 101 107 61 pint:PI1751|3end_desulfoferrodoxin_1680280..1680430_POSITIVE 223 89 44 36 4 pint:PI1008|3end_cytochrome_c_nitrite_reductase,_small_subunit_929331..929481_POSITIVE 222 117 82 104 60 pint:PI0805|3end_conserved_hypothetical_protein_743807..743957_POSITIVE 222 147 104 76 30 pint:PI0156|3end_conserved_hypothetical_protein_150913..151063_POSITIVE 221 87 43 151 102 pint:PI1497|3end_LuxS_protein,_autoinducer_AI-2_production_protein_1418319..1418469_POSITIVE 217 146 104 131 93 pint:PI1448|3end_hypothetical_protein_1371832..1371982_POSITIVE 217 130 84 91 51 pint:PI0605|3end_chloromuconate_cycloisomerase_553026..553176_POSITIVE 215 115 78 128 97 pint:PI1517|3end_AcrB/AcrD_family_multidrug_resistance_protein_1438973..1439123_POSITIVE 215 219 178 95 52 pint:PI0952|3end_conserved_hypothetical_protein_884775..884925_POSITIVE 213 150 101 101 56 pint:PI1198|3end_acetyltransferase_1115219..1115369_POSITIVE 212 119 74 135 87 pint:PI0459|3end_30S_ribosomal_protein_S10_432467..432617_POSITIVE 210 110 66 92 42 pint:PI1590|3end_transcriptional_regulator_1518498..1518648_POSITIVE 210 136 100 75 30 pint:PI0276|3end_anti-restriction_protein_265021..265171_POSITIVE