Origin IGS: aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatcggtttatggtgggatggaaagaaaagaagagggtatgtttttctttctttcccggtcgtagataatttgttatcaataatctaaaagaggaatgtt Mask Tandem Repeat Region ================================================ aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Find is-nt database================================================ Query_seq: AA02983:AA02984|AA02983:AA02984:formate acetyltransferase:formate transporter:->->:2087645..2087745 101 aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02983:AA02984|AA02983:AA02984:formate acetyltransferase:formate transporter:->->:2087645..2087745 101 aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02983:AA02984|AA02983:AA02984:formate acetyltransferase:formate transporter:->->:2087645..2087745 101 aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Predict ORF larger than 30AA ================================================ aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02983:AA02984|AA02983:AA02984:formate acetyltransferase:formate transporter:->->:2087645..2087745 101 aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Intra-Species Hit: Count: 1 Min: 1 Max: 101 Len: 101 Subject: aact_AA02983_AA02984|formate acetyltransferase:formate transporter|POSITIVE:POSITIVE|[2087645,2087745]|101 HSP 1 e-value: 6.0E-51 bit: 200.0 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 1 Subject End: 101 aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta aacattcctcttttagattattgataacaaattatctacgaccgggaaagaaagaaaaacataccctcttcttttctttccatcccaccataaaccgatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAUUCCUCUUUUAGAUUAUUGAUAACAAAUUAUCUACGACCGGGAAAGAAAGAAAAACAUACCCUCUUCUUUUCUUUCCAUCCCACCAUAAACCGAUUA .............(((((.(((.......))).))))).....((((..(((((((((.............)))))))))..))))............... (-17.02) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCGGUUUAUGGUGGGAUGGAAAGAAAAGAAGAGGGUAUGUUUUUCUUUCUUUCCCGGUCGUAGAUAAUUUGUUAUCAAUAAUCUAAAAGAGGAAUGUU ......(((((((((((((.(((((((((((...........))))))))))).)))).)))))))))...........((((.(((......))).)))) (-21.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 261 75 36 220 176 aact:AA01947|5end_iron(III)-transport_ATP-binding_protein_1316390..1316620_POSITIVE 251 91 56 203 167 aact:AA01967|5end_hypothetical_protein_1331511..1331741_POSITIVE 228 89 60 227 188 aact:AA02375|5end_D-3-phosphoglycerate_dehydrogenase_1626514..1626744_POSITIVE 222 96 64 222 183 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 218 73 39 104 75 aact:AA02434|5end_membrane_protein_1678547..1678777_POSITIVE 214 84 41 123 80 aact:AA01299|5end_competence_protein_C_880946..881176_POSITIVE 211 65 27 192 147 aact:AA01675|5end_probable_multidrug_resistance_protein;_multidrug-efflux_transporter_1132115..1132345_POSITIVE 211 65 27 94 49 aact:AA01674|5end_hypothetical_protein_1132017..1132247_POSITIVE 205 95 62 227 193 aact:AA01635|5end_ribonucleoside_diphosphate_reductase,_beta_chain_1104615..1104845_POSITIVE 201 89 41 228 172 aact:AA02910|5end_ribonuclease_E_2036137..2036367_POSITIVE 199 98 67 190 154 aact:AA00416|5end_D-methionine_transport_system_permease_protein_(ABC_transport_system)_282677..282907_POSITIVE 196 80 38 115 65 aact:AA02526|5end_conserved_hypothetical_protein_1755225..1755455_POSITIVE 196 57 26 72 42 aact:AA01154|5end_sec-independent_protein_translocase_protein_790832..791062_POSITIVE 194 45 27 18 1 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 193 97 78 189 170 aact:AA00979|5end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_684645..684875_POSITIVE 192 99 75 31 7 aact:AA01158|5end_ribosome_associated_heat_shock_protein_15_homolog_792399..792629_POSITIVE 191 97 64 212 182 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 190 98 60 73 33 aact:AA02075|5end_conserved_hypothetical_protein_1409654..1409884_POSITIVE 190 100 79 105 74 aact:AA00218|5end_argininosuccinate_lyase_150758..150988_POSITIVE 189 41 3 86 48 aact:AA02683|5end_hydrogenase_4_Fe-S_subunit_1868882..1869112_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 91 56 126 90 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 238 68 26 75 40 aact:AA02674|3end_formate_dehydrogenase-related_protein_1860732..1860882_POSITIVE 222 96 64 106 67 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 214 84 41 120 77 aact:AA01298|3end_competence_protein_D_881023..881173_POSITIVE 212 97 66 119 86 aact:AA01402|3end_beta_1-4_glucosyltransferase_948382..948532_POSITIVE 211 65 27 90 45 aact:AA01673|3end_riboflavin_synthase,_alpha_chain_1132093..1132243_POSITIVE 198 66 23 142 85 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 196 80 38 112 62 aact:AA02525|3end_conserved_hypothetical_protein_1755302..1755452_POSITIVE 196 57 26 65 35 aact:AA01153|3end_sec-independent_protein_translocase_790905..791055_POSITIVE 193 97 78 49 30 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 191 97 64 62 32 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 190 100 79 92 61 aact:AA00217|3end_hypothetical_protein_150825..150975_POSITIVE 189 49 1 131 94 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 188 90 42 105 58 aact:AA00734|3end_citrate_lyase,_gamma_chain_(acyl_carrier_protein)_502754..502904_POSITIVE 183 86 42 139 79 aact:AA02270|3end_endopeptidase_La;_lon_protease;_protease_La_homolog;_ATP-dependent_protease_LA_(lon-1)_1548668..1548818_POSITIVE 180 99 58 120 73 aact:AA02687|3end_hydrogenase-4_component_D_1874043..1874193_POSITIVE 180 55 24 106 71 aact:AA01689|3end_conserved_hypothetical_protein_(possible_inner_membrane_protein)_1146665..1146815_POSITIVE 180 86 41 121 71 aact:AA01396|3end_saccharide_biosynthesis_regulatory_protein_944880..945030_POSITIVE 179 99 67 113 81 aact:AA00603|3end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410992..411142_POSITIVE 177 72 31 73 30 aact:AA03015|3end_hypothetical_protein_2105251..2105401_POSITIVE