Origin IGS: gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgattgggtagtaaaaataccttaatatctcagccgcttaccccgtggtaagcggtctgattttttctgtcatttaaacatgttggggaaagtaaaatgattaccgtcgtactttttcgctatttatgacgatctattggggaatac Mask Tandem Repeat Region ================================================ gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Find is-nt database================================================ Query_seq: AA02982:AA02983|AA02982:AA02983:pyruvate formate-lyase activating enzyme:formate acetyltransferase:->->:2085188..2085334 147 gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02982:AA02983|AA02982:AA02983:pyruvate formate-lyase activating enzyme:formate acetyltransferase:->->:2085188..2085334 147 gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02982:AA02983|AA02982:AA02983:pyruvate formate-lyase activating enzyme:formate acetyltransferase:->->:2085188..2085334 147 gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Predict ORF larger than 30AA ================================================ gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -14.9 Tail_Score: -5.43487 Start: 88 End: 118 Full_Region: aaataccttaatatc tcagccgcttacc ccgt ggtaagcggtctga ttttttctgtcattt .......................................................................................tcagccgcttaccccgtggtaagcggtctga............................. Find igs database================================================ Query_seq: AA02982:AA02983|AA02982:AA02983:pyruvate formate-lyase activating enzyme:formate acetyltransferase:->->:2085188..2085334 147 gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Intra-Species Hit: Count: 1 Min: 1 Max: 147 Len: 147 Subject: aact_AA02982_AA02983|pyruvate formate-lyase activating enzyme:formate acetyltransferase|POSITIVE:POSITIVE|[2085188, 2085334]|147 HSP 1 e-value: 3.0E-78 bit: 291.0 Len: 147 Query Start:1 Query End:147 Subject Strand: POSITIVE Subject Start: 1 Subject End: 147 gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca gtattccccaatagatcgtcataaatagcgaaaaagtacgacggtaatcattttactttccccaacatgtttaaatgacagaaaaaatcagaccgcttaccacggggtaagcggctgagatattaaggtatttttactacccaatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUAUUCCCCAAUAGAUCGUCAUAAAUAGCGAAAAAGUACGACGGUAAUCAUUUUACUUUCCCCAACAUGUUUAAAUGACAGAAAAAAUCAGACCGCUUACCACGGGGUAAGCGGCUGAGAUAUUAAGGUAUUUUUACUACCCAAUCA .............(((.(((((.....((......))..((.(((((.....))))).))..............)))))........((((.(((((((((....)))))))))))))........((((.......))))..))). (-33.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUGGGUAGUAAAAAUACCUUAAUAUCUCAGCCGCUUACCCCGUGGUAAGCGGUCUGAUUUUUUCUGUCAUUUAAACAUGUUGGGGAAAGUAAAAUGAUUACCGUCGUACUUUUUCGCUAUUUAUGACGAUCUAUUGGGGAAUAC ...................((((((((..(((((((((((((....))))))))).)))).......((((((........((..(..((((((..(((.....)))..))))))..))).....))))))...))))))))..... (-41.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 356 119 76 160 106 aact:AA01472|5end_hypothetical_protein_988896..989126_POSITIVE 324 115 91 67 41 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 316 122 89 93 55 aact:AA00506|5end_ATP-dependent_RNA_helicase_protein_351681..351911_POSITIVE 305 118 79 156 118 aact:AA00534|5end_hypothetical_protein_365419..365649_POSITIVE 305 118 79 60 22 aact:AA00533|5end_conserved_hypothetical_protein_365323..365553_POSITIVE 295 130 90 167 132 aact:AA02407|5end_thymidine_kinase_1652666..1652896_POSITIVE 292 120 71 118 69 aact:AA02826|5end_conserved_hypothetical_protein_1986336..1986566_POSITIVE 292 120 89 56 28 aact:AA00290|5end_conserved_hypothetical_protein_201272..201502_POSITIVE 288 137 93 41 6 aact:AA00251|5end_lipoate_biosynthesis_protein_B_176523..176753_POSITIVE 284 130 87 123 68 aact:AA01159|5end_phosphatase_792828..793058_POSITIVE 281 117 86 217 184 aact:AA02337|5end_DNA_mismatch_repair_protein_1597350..1597580_POSITIVE 278 120 90 228 186 aact:AA00790|5end_recombination_DNA_repair_protein_542455..542685_POSITIVE 276 128 102 38 13 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 272 115 73 89 49 aact:AA02965|5end_hypothetical_protein_2074069..2074299_POSITIVE 271 138 94 172 127 aact:AA00675|5end_glutaredoxin_2_462524..462754_POSITIVE 270 117 73 69 24 aact:AA02846|5end_crossover_junction_endodeoxyribonuclease_1998648..1998878_POSITIVE 269 119 71 70 20 aact:AA00835|5end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_575212..575442_POSITIVE 269 69 27 150 108 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 269 130 89 98 64 aact:AA00012|5end_conserved_hypothetical_protein_6433..6663_POSITIVE 267 129 90 67 15 aact:AA01835|5end_conserved_hypothetical_protein_1234896..1235126_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 316 136 91 145 100 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 316 122 89 65 27 aact:AA00505|3end_hypothetical_protein_351733..351883_POSITIVE 305 118 79 42 4 aact:AA00531|3end_DNA_polymerase_I_365385..365535_POSITIVE 301 128 86 147 102 aact:AA00785|3end_conserved_hypothetical_protein_(possible_integrase)_539672..539822_POSITIVE 293 120 79 151 103 aact:AA02404|3end_hypothetical_protein_1652736..1652886_POSITIVE 292 120 71 101 52 aact:AA02824|3end_conserved_hypothetical_protein_1986399..1986549_POSITIVE 292 120 89 51 23 aact:AA00288|3end_conserved_hypothetical_protein_201347..201497_POSITIVE 284 130 87 107 52 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 272 115 73 68 28 aact:AA02964|3end_opacity_associated_protein_A_2074128..2074278_POSITIVE 269 126 85 142 104 aact:AA00860|3end_phosphoheptose_isomerase_592527..592677_POSITIVE 269 69 27 147 105 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 265 118 75 138 91 aact:AA03010|3end_thiophene_and_furan_oxidation_protein_2102534..2102684_POSITIVE 263 120 78 151 104 aact:AA00976|3end_conserved_hypothetical_protein_682278..682428_POSITIVE 261 139 92 131 80 aact:AA00970|3end_uroporphyrinogen_decarboxylase_(uroporphyrinogen_III_decarboxylase)_677267..677417_POSITIVE 261 120 92 98 68 aact:AA00744|3end_aldose_1-epimerase_(mutarotase)_511246..511396_POSITIVE 261 115 78 60 4 aact:AA00314|3end_conserved_hypothetical_protein_(possible_Kmt1_protein)_214326..214476_POSITIVE 259 117 83 137 110 aact:AA01877|3end_conserved_hypothetical_protein_1264890..1265040_POSITIVE 257 121 87 37 3 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 256 133 94 71 19 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 255 122 81 115 71 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE