Origin IGS: gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatccggatcgaacattgcctgatctgaaaaatgttttgtaaaacgaccgcacttttagcacaaataaacccatttcctcgtttacgctgttatcaaagcgcactacaatagtcgctcacctcccttctttttaaggaatctc Mask Tandem Repeat Region ================================================ gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Find is-nt database================================================ Query_seq: AA02980:AA02982|AA02980:AA02982:conserved hypothetical protein:pyruvate formate-lyase activating enzyme:->->:2084306..2084449 144 gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02980:AA02982|AA02980:AA02982:conserved hypothetical protein:pyruvate formate-lyase activating enzyme:->->:2084306..2084449 144 gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02980:AA02982|AA02980:AA02982:conserved hypothetical protein:pyruvate formate-lyase activating enzyme:->->:2084306..2084449 144 gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 34 End: 144 ................................. M V V R F D N S V N E E M G L F V L K V R S F Y K T F F R S G N V R S G L gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02980:AA02982|AA02980:AA02982:conserved hypothetical protein:pyruvate formate-lyase activating enzyme:->->:2084306..2084449 144 gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Intra-Species Hit: Count: 1 Min: 1 Max: 144 Len: 144 Subject: aact_AA02980_AA02982|conserved hypothetical protein:pyruvate formate-lyase activating enzyme|POSITIVE:POSITIVE|[2084306,2084449]|144 HSP 1 e-value: 2.0E-76 bit: 285.0 Len: 144 Query Start:1 Query End:144 Subject Strand: POSITIVE Subject Start: 1 Subject End: 144 gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta gagattccttaaaaagaagggaggtgagcgactattgtagtgcgctttgataacagcgtaaacgaggaaatgggtttatttgtgctaaaagtgcggtcgttttacaaaacatttttcagatcaggcaatgttcgatccggatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGAUUCCUUAAAAAGAAGGGAGGUGAGCGACUAUUGUAGUGCGCUUUGAUAACAGCGUAAACGAGGAAAUGGGUUUAUUUGUGCUAAAAGUGCGGUCGUUUUACAAAACAUUUUUCAGAUCAGGCAAUGUUCGAUCCGGAUUA ....((((((......)))))).((((((((((........(((((((....((((.((((((..........))))))))))....)))))))))))).))))).........(((.((((..((...))..)))).)))... (-33.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCGGAUCGAACAUUGCCUGAUCUGAAAAAUGUUUUGUAAAACGACCGCACUUUUAGCACAAAUAAACCCAUUUCCUCGUUUACGCUGUUAUCAAAGCGCACUACAAUAGUCGCUCACCUCCCUUCUUUUUAAGGAAUCUC .....(((((((.........))))))).................((((.((.......))....................((...((((........))))....))....))))........((((......))))...... (-18.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 406 100 52 166 121 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 372 126 86 161 122 aact:AA00721|5end_probable_beta-N-acetylhexosaminidase_(lacto-N-biosidase);_dispersin_B_494739..494969_POSITIVE 372 126 86 49 10 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 367 70 21 179 128 aact:AA01756|5end_cobalt_membrane_transport_protein_1186475..1186705_POSITIVE 365 103 66 118 88 aact:AA02618|5end_phosphatidylglycerophosphatase_B_1824649..1824879_POSITIVE 353 140 91 179 125 aact:AA00356|5end_DNA-directed_RNA_polymerase,_beta'_chain_238937..239167_POSITIVE 342 128 89 38 1 aact:AA02415|5end_hypothetical_protein_1658443..1658673_POSITIVE 338 126 83 143 101 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 337 99 53 159 120 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 335 103 62 94 59 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 334 118 78 123 82 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 334 118 78 54 13 aact:AA02096|5end_hypothetical_protein_1428754..1428984_POSITIVE 333 80 31 172 127 aact:AA02082|5end_glutaminyl-tRNA_synthetase_1416101..1416331_POSITIVE 333 97 51 165 112 aact:AA00395|5end_conserved_hypothetical_protein_265278..265508_POSITIVE 330 97 54 80 29 aact:AA02482|5end_conserved_hypothetical_protein_1720131..1720361_POSITIVE 327 64 28 119 81 aact:AA01158|5end_ribosome_associated_heat_shock_protein_15_homolog_792399..792629_POSITIVE 323 128 84 149 102 aact:AA00458|5end_nickel-binding_periplasmic_protein_319374..319604_POSITIVE 322 101 79 140 118 aact:AA02836|5end_colicin_transport_protein_1991777..1992007_POSITIVE 322 101 79 64 42 aact:AA02835|5end_hypothetical_protein_1991701..1991931_POSITIVE 322 103 69 200 171 aact:AA02486|5end_topoisomerase_IV,_subunit_B_1724337..1724567_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 406 100 52 83 38 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 372 126 86 65 26 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 371 128 86 67 27 aact:AA02412|3end_cysteine_synthetase_1658552..1658702_POSITIVE 365 103 66 64 34 aact:AA02616|3end_transcriptional_regulator_1824675..1824825_POSITIVE 353 140 91 73 19 aact:AA00355|3end_DNA-directed_RNA_polymerase_beta_chain_(transcriptase_beta_chain)_238911..239061_POSITIVE 338 126 83 77 35 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 337 99 53 100 61 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 335 103 62 88 53 aact:AA00990|3end_conserved_hypothetical_protein_693265..693415_POSITIVE 333 97 51 55 2 aact:AA00391|3end_arsenate_reductase_265248..265398_POSITIVE 327 110 65 140 97 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 327 64 28 48 10 aact:AA01156|3end_chaperonin_heat_shock_protein_33_homolog_792408..792558_POSITIVE 325 120 71 135 76 aact:AA01597|3end_oligoribonuclease_1078936..1079086_POSITIVE 323 128 84 80 33 aact:AA00455|3end_lipoprotein_precursor_319385..319535_POSITIVE 322 101 79 51 29 aact:AA02834|3end_colicin_transport_protein_1991768..1991918_POSITIVE 314 98 56 82 40 aact:AA02063|3end_23S_rRNA_psedouridine_synthase,_Rlu_family_protein_1405217..1405367_POSITIVE 308 100 52 83 40 aact:AA01270|3end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863897..864047_POSITIVE 308 127 88 120 83 aact:AA00064|3end_ribosomal_small_subunit_pseudouridine_synthase_48946..49096_POSITIVE 306 99 51 118 79 aact:AA01474|3end_hypothetical_protein_990431..990581_POSITIVE 301 117 82 74 39 aact:AA01537|3end_possible_glucokinase_regulatory_protein_1031607..1031757_POSITIVE 299 99 55 75 36 aact:AA02296|3end_conserved_hypothetical_protein_(possible_cell_wall-associated_hydrolase)_1566581..1566731_POSITIVE