Origin IGS: aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtaaaacctcttaaaattttgcctcagtatagcgaaattaaccaattaagtgtattaataagtactaaatgtgctaatctaacggaaaaatttcttgaattaaattcct Mask Tandem Repeat Region ================================================ aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Find is-nt database================================================ Query_seq: AA02976:AA02977|AA02976:AA02977:UDP-2,3-diacylglucosamine hydrolase:1-acyl-sn-glycerol-3-phosphate acyltransferase:->->:2081098..2081207 110 aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02976:AA02977|AA02976:AA02977:UDP-2,3-diacylglucosamine hydrolase:1-acyl-sn-glycerol-3-phosphate acyltransferase:->->:2081098..2081207 110 aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02976:AA02977|AA02976:AA02977:UDP-2,3-diacylglucosamine hydrolase:1-acyl-sn-glycerol-3-phosphate acyltransferase:->->:2081098..2081207 110 aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 2 End: 94 . S N L E L F K E T L N A C K T S I L V S L Q N I E S Y Q P L M ................ aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02976:AA02977|AA02976:AA02977:UDP-2,3-diacylglucosamine hydrolase:1-acyl-sn-glycerol-3-phosphate acyltransferase:->->:2081098..2081207 110 aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Intra-Species Hit: Count: 1 Min: 1 Max: 110 Len: 110 Subject: aact_AA02976_AA02977|UDP-2,3-diacylglucosamine hydrolase:1-acyl-sn-glycerol-3-phosphate acyltransferase|POSITIVE:POSITIVE|[2081098,2081207]|110 HSP 1 e-value: 3.0E-56 bit: 218.0 Len: 110 Query Start:1 Query End:110 Subject Strand: POSITIVE Subject Start: 1 Subject End: 110 aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc aggaatttaattcaagaaatttttccgttagattagcacatttagtacttattaatacacttaattggttaatttcgctatactgaggcaaaattttaagaggttttacc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGAAUUUAAUUCAAGAAAUUUUUCCGUUAGAUUAGCACAUUUAGUACUUAUUAAUACACUUAAUUGGUUAAUUUCGCUAUACUGAGGCAAAAUUUUAAGAGGUUUUACC .((.......(((..((((((((.((.((((..((((....(((..(((.(((((.....))))).))))))....))))..)))))).))))))))..)))......)) (-15.82) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUAAAACCUCUUAAAAUUUUGCCUCAGUAUAGCGAAAUUAACCAAUUAAGUGUAUUAAUAAGUACUAAAUGUGCUAAUCUAACGGAAAAAUUUCUUGAAUUAAAUUCCU (((((((..........)))))))..........((((((..((.(((((.....))))).(((((.....)))))........))...))))))............... (-14.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 232 89 45 116 74 aact:AA01460|5end_hypothetical_protein_981412..981642_POSITIVE 212 90 65 139 118 aact:AA00349|5end_hypothetical_protein_233274..233504_POSITIVE 206 110 75 221 187 aact:AA02621|5end_hypothetical_protein_1827104..1827334_POSITIVE 197 70 24 211 171 aact:AA00003|5end_DNA_replication_and_repair_protein_2337..2567_POSITIVE 196 108 65 225 184 aact:AA02384|5end_2-dehydro-3-deoxygluconokinase_1632112..1632342_POSITIVE 195 86 45 231 192 aact:AA01462|5end_xylose_operon_regulatory_protein_981530..981760_POSITIVE 195 89 59 97 70 aact:AA00040|5end_thiol:disulfide_interchange_protein_31358..31588_POSITIVE 193 50 14 148 116 aact:AA01802|5end_hypothetical_protein_1214432..1214662_POSITIVE 193 109 65 105 56 aact:AA00723|5end_peptidase_B_(leucyl_aminopeptidase)_496457..496687_POSITIVE 191 91 61 64 34 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 190 106 65 102 57 aact:AA00643|5end_hypothetical_protein_440432..440662_POSITIVE 188 79 31 192 134 aact:AA01153|5end_sec-independent_protein_translocase_790166..790396_POSITIVE 186 90 54 35 3 aact:AA01508|5end_anaerobic_C4-dicarboxylate_transporter_1013198..1013428_POSITIVE 184 104 76 219 197 aact:AA00214|5end_glutamyl-tRNA_synthetase_147727..147957_POSITIVE 183 110 76 102 65 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 179 104 66 75 31 aact:AA00388|5end_uridine_phosphorylase;_possible_5'-methylthioadenosine_phosphorylase;_purine_nucleoside_phosphorylase_262791..263021_POSITIVE 178 107 65 141 87 aact:AA02873|5end_conserved_hypothetical_protein_2016165..2016395_POSITIVE 178 78 31 125 79 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 177 92 56 112 71 aact:AA02109|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436563..1436793_POSITIVE 177 110 67 191 147 aact:AA01404|5end_hypothetical_protein_948578..948808_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 232 89 45 116 74 aact:AA01459|3end_Na(+)/H(+)_antiporter_981492..981642_POSITIVE 212 90 65 121 100 aact:AA00348|3end_50S_ribosomal_protein_L1_233336..233486_POSITIVE 196 80 40 146 106 aact:AA01722|3end_conserved_hypothetical_protein_1163639..1163789_POSITIVE 195 89 59 97 70 aact:AA00039|3end_cytochrome_c-type_biogenesis_protein_31438..31588_POSITIVE 193 109 65 90 41 aact:AA00722|3end_nucleoside_diphosphate_kinase_496522..496672_POSITIVE 192 69 22 57 13 aact:AA01272|3end_hypothetical_protein_864211..864361_POSITIVE 190 106 65 52 7 aact:AA00642|3end_conserved_hypothetical_protein_440462..440612_POSITIVE 188 79 31 151 93 aact:AA01152|3end_sec-independent_protein_translocase_protein_790205..790355_POSITIVE 178 107 65 78 24 aact:AA02872|3end_conserved_hypothetical_protein_2016182..2016332_POSITIVE 178 78 31 144 98 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 177 92 56 112 71 aact:AA02108|3end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436643..1436793_POSITIVE 177 104 73 70 33 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 177 109 65 90 32 aact:AA00390|3end_permease_264827..264977_POSITIVE 175 39 1 131 98 aact:AA00619|3end_hypothetical_protein_424324..424474_POSITIVE 175 53 20 113 75 aact:AA00169|3end_conserved_hypothetical_protein_120943..121093_POSITIVE 172 90 43 136 80 aact:AA02316|3end_exodeoxyribonuclease_VII_large_subunit_1583093..1583243_POSITIVE 172 58 19 128 92 aact:AA01533|3end_conserved_hypothetical_protein_1029451..1029601_POSITIVE 169 110 67 142 91 aact:AA02802|3end_probable_myo-inositol_2-dehydrogenase_1970086..1970236_POSITIVE 169 105 66 150 113 aact:AA02516|3end_ferric_uptake_regulation_protein_1749781..1749931_POSITIVE 169 89 61 127 104 aact:AA02490|3end_hypothetical_protein_1726896..1727046_POSITIVE