Origin IGS: tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gagttttctcctttcctgtttccgtagaaaggctagtcacttccttatttgacaatacatcaaactacaatgtcactagaatgttttcgcattttcctcattaaaattctgacgggatataagaagcccttactccgtttccgaagtaagggctgatatgacta Mask Tandem Repeat Region ================================================ tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Find is-nt database================================================ Query_seq: AA02972:AA02973|AA02972:AA02973:conserved hypothetical protein (tyrosine phenol lyase-related protein):tyrosine-specific transport protein:->->:2077683..2077846 164 tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02972:AA02973|AA02972:AA02973:conserved hypothetical protein (tyrosine phenol lyase-related protein):tyrosine-specific transport protein:->->:2077683..2077846 164 tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02972:AA02973|AA02972:AA02973:conserved hypothetical protein (tyrosine phenol lyase-related protein):tyrosine-specific transport protein:->->:2077683..2077846 164 tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 12 End: 116 ........... G K S R F R L L P K K Y G T L I K I L F I R F C E L S M T T Q H I T M ................................................ tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02972:AA02973|AA02972:AA02973:conserved hypothetical protein (tyrosine phenol lyase-related protein):tyrosine-specific transport protein:->->:2077683..2077846 164 tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Intra-Species Hit: Count: 1 Min: 1 Max: 164 Len: 164 Subject: aact_AA02972_AA02973|conserved hypothetical protein (tyrosine phenol lyase-related protein):tyrosine-specific transport protein|POSITIVE:POSITIVE|[2077683,2077846]|164 HSP 1 e-value: 3.0E-88 bit: 325.0 Len: 164 Query Start:1 Query End:164 Subject Strand: POSITIVE Subject Start: 1 Subject End: 164 tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc tagtcatatcagcccttacttcggaaacggagtaagggcttcttatatcccgtcagaattttaatgaggaaaatgcgaaaacattctagtgacattgtagtttgatgtattgtcaaataaggaagtgactagcctttctacggaaacaggaaaggagaaaactc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUCAUAUCAGCCCUUACUUCGGAAACGGAGUAAGGGCUUCUUAUAUCCCGUCAGAAUUUUAAUGAGGAAAAUGCGAAAACAUUCUAGUGACAUUGUAGUUUGAUGUAUUGUCAAAUAAGGAAGUGACUAGCCUUUCUACGGAAACAGGAAAGGAGAAAACUC (((((((...(((((((((((((....))))))))))))).......(((.((((..(((((.......)))))..(((....)))...))))......(((((((.....)))))))..))).))))))).(((((((..(....).)))))))......... (-52.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGUUUUCUCCUUUCCUGUUUCCGUAGAAAGGCUAGUCACUUCCUUAUUUGACAAUACAUCAAACUACAAUGUCACUAGAAUGUUUUCGCAUUUUCCUCAUUAAAAUUCUGACGGGAUAUAAGAAGCCCUUACUCCGUUUCCGAAGUAAGGGCUGAUAUGACUA .((((.((....(((.(((((((((((((...(((((.((.......(((((.......))))).......)).))))).((((....))))..............)))).)))))).))).)))(((((((((.((....)).))))))))).))...)))). (-36.94) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 328 56 12 38 2 aact:AA00914|5end_aminoacyl-histidine_dipeptidase;_Beta-alanyl-histidine_dipeptidase_628038..628268_POSITIVE 293 69 21 197 147 aact:AA00141|5end_hypoxanthine_phosphoribosyl_transferase_(HPRT)_100814..101044_POSITIVE 290 69 21 215 174 aact:AA00841|5end_UDP-MurNAc-tripeptide_synthetase_581318..581548_POSITIVE 278 69 23 42 2 aact:AA00209|5end_D-ribose_ABC_transporter,_periplasmic-binding_protein_145083..145313_POSITIVE 277 39 1 130 85 aact:AA01231|5end_30S_ribosomal_protein_S14_840103..840333_POSITIVE 272 70 21 88 38 aact:AA00654|5end_conserved_hypothetical_protein_447772..448002_POSITIVE 265 55 21 168 136 aact:AA01428|5end_acetolactate_synthase_958688..958918_POSITIVE 264 52 12 227 186 aact:AA01735|5end_hypothetical_protein_1172533..1172763_POSITIVE 263 93 52 185 147 aact:AA00552|5end_periplasmic_nitrate_reductase_precursor_377331..377561_POSITIVE 263 93 52 79 41 aact:AA00551|5end_hypothetical_protein_377225..377455_POSITIVE 253 59 22 229 191 aact:AA00196|5end_conserved_hypothetical_protein_137378..137608_POSITIVE 251 58 14 150 105 aact:AA00927|5end_ATP-dependent_DNA_helicase_635811..636041_POSITIVE 250 120 74 208 155 aact:AA02000|5end_2,3,4,5-tetrahydropyridine-2-carboxylate_1358897..1359127_POSITIVE 248 55 11 176 149 aact:AA01193|5end_AMP-binding_enzyme_817928..818158_POSITIVE 248 41 11 133 107 aact:AA00722|5end_nucleoside_diphosphate_kinase_496020..496250_POSITIVE 247 129 83 55 16 aact:AA01062|5end_ribonuclease_PH_(RNase_PH)_735706..735936_POSITIVE 244 77 31 179 124 aact:AA02145|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1468602..1468832_POSITIVE 243 99 51 212 165 aact:AA01573|5end_peptide_transport_periplasmic_protein_1059761..1059991_POSITIVE 242 156 124 72 38 aact:AA02319|5end_nitrogen_regulatory_IIA_protein_1583907..1584137_POSITIVE 242 55 12 224 162 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 428 40 11 80 51 aact:AA02972|3end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077622..2077772_POSITIVE 277 39 1 114 69 aact:AA01230|3end_50S_ribosomal_protein_L5_840167..840317_POSITIVE 272 70 21 96 46 aact:AA00653|3end_hypothetical_protein_447860..448010_POSITIVE 265 55 21 55 23 aact:AA01426|3end_threonine_deaminase_(dehydratase)_958655..958805_POSITIVE 251 58 14 150 105 aact:AA00926|3end_CyaY_protein_635891..636041_POSITIVE 250 120 74 120 67 aact:AA01999|3end_cys_regulon_transcriptional_activator;_cysteine_synthase_1358889..1359039_POSITIVE 248 55 11 145 118 aact:AA01192|3end_conserved_hypothetical_protein_(possible_membrane_protein)_817977..818127_POSITIVE 247 129 83 42 3 aact:AA01059|3end_orotate_phosphoribosyltransferase_735773..735923_POSITIVE 246 46 4 88 37 aact:AA02118|3end_hypothetical_protein_1445939..1446089_POSITIVE 244 157 113 56 12 aact:AA00782|3end_conserved_hypothetical_protein_539120..539270_POSITIVE 240 57 12 125 80 aact:AA02819|3end_DNA_polymerase_III,_psi_subunit_1982322..1982472_POSITIVE 239 59 13 54 13 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 239 59 13 54 13 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 235 44 11 143 109 aact:AA00700|3end_iron(III)_ABC_transporter,_ATP-binding_protein_481458..481608_POSITIVE 234 40 2 47 20 aact:AA02421|3end_conserved_hypothetical_protein_1667300..1667450_POSITIVE 234 40 2 76 49 aact:AA02297|3end_conserved_hypothetical_protein_1569900..1570050_POSITIVE 234 40 2 76 49 aact:AA00621|3end_conserved_hypothetical_protein_429007..429157_POSITIVE 234 40 2 76 49 aact:AA00412|3end_conserved_hypothetical_protein_279454..279604_POSITIVE 234 58 28 104 77 aact:AA00196|3end_conserved_hypothetical_protein_138015..138165_POSITIVE 233 49 5 150 105 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE