Origin IGS: aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cacaggctcacgtttggctaaatcgccacgcatcggaaagcccgtttcgggaaggttaagggtatttttataatcagtcattttctattccagttttgtgtaaaaatttaaatttattttaagccacttgtt Mask Tandem Repeat Region ================================================ aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Find is-nt database================================================ Query_seq: AA02949:AA02950|AA02949:AA02950:hypothetical protein:isoleucyl-tRNA synthetase:->->:2062303..2062434 132 aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02949:AA02950|AA02949:AA02950:hypothetical protein:isoleucyl-tRNA synthetase:->->:2062303..2062434 132 aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02949:AA02950|AA02949:AA02950:hypothetical protein:isoleucyl-tRNA synthetase:->->:2062303..2062434 132 aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Predict ORF larger than 30AA ================================================ aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02949:AA02950|AA02949:AA02950:hypothetical protein:isoleucyl-tRNA synthetase:->->:2062303..2062434 132 aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Intra-Species Hit: Count: 1 Min: 1 Max: 132 Len: 132 Subject: aact_AA02949_AA02950|hypothetical protein:isoleucyl-tRNA synthetase|POSITIVE:POSITIVE|[2062303,2062434]|132 HSP 1 e-value: 3.0E-69 bit: 262.0 Len: 132 Query Start:1 Query End:132 Subject Strand: POSITIVE Subject Start: 1 Subject End: 132 aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg aacaagtggcttaaaataaatttaaatttttacacaaaactggaatagaaaatgactgattataaaaatacccttaaccttcccgaaacgggctttccgatgcgtggcgatttagccaaacgtgagcctgtg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAAGUGGCUUAAAAUAAAUUUAAAUUUUUACACAAAACUGGAAUAGAAAAUGACUGAUUAUAAAAAUACCCUUAACCUUCCCGAAACGGGCUUUCCGAUGCGUGGCGAUUUAGCCAAACGUGAGCCUGUG .....(((....(((((........)))))..)))....(.((((..................................)))).)..((((((((....(((..((((......))))..))))))))))). (-22.78) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CACAGGCUCACGUUUGGCUAAAUCGCCACGCAUCGGAAAGCCCGUUUCGGGAAGGUUAAGGGUAUUUUUAUAAUCAGUCAUUUUCUAUUCCAGUUUUGUGUAAAAAUUUAAAUUUAUUUUAAGCCACUUGUU ....((((...((.((((......)))).))...((((..((((...))))..(((((..((....))..)))))............))))..............................))))....... (-24.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 404 129 82 55 9 aact:AA01790|5end_riboflavin_bifunctional_biosynthesis_protein_1206934..1207164_POSITIVE 386 130 83 182 127 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 368 118 72 141 100 aact:AA02688|5end_hydrogenase_4_component_E_1873977..1874207_POSITIVE 365 130 82 214 148 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 362 127 82 173 123 aact:AA02705|5end_hypothetical_protein_1886717..1886947_POSITIVE 361 130 83 189 138 aact:AA01072|5end_GTP_pyrophosphokinase_(ppGpp_synthetase_I)_/_ATP:GTP3'-pyrophosphotransferase_742934..743164_POSITIVE 355 130 83 65 9 aact:AA02609|5end_ABC_transporter,_ATP-binding_protein/permease_1818446..1818676_POSITIVE 340 130 87 174 112 aact:AA02141|5end_ABC_transporter,_permease_protein_1464890..1465120_POSITIVE 340 128 91 57 20 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 339 130 88 69 33 aact:AA00578|5end_DNA_topoisomerase_394576..394806_POSITIVE 336 129 82 130 85 aact:AA02078|5end_hypothetical_protein_1412612..1412842_POSITIVE 333 130 82 66 19 aact:AA00608|5end_protective_surface_antigen_D-15,_85_-kilodalton_outermembrane_protein_413161..413391_POSITIVE 330 117 71 141 101 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 328 128 85 163 116 aact:AA00012|5end_conserved_hypothetical_protein_6433..6663_POSITIVE 326 120 71 180 131 aact:AA00521|5end_phosphatase_357744..357974_POSITIVE 324 129 81 201 145 aact:AA02678|5end_formate_dehydrogenase,_gamma_subunit_1865021..1865251_POSITIVE 324 129 81 225 167 aact:AA01973|5end_acetate_kinase_1334424..1334654_POSITIVE 323 127 84 108 60 aact:AA01426|5end_threonine_deaminase_(dehydratase)_958207..958437_POSITIVE 322 110 69 71 20 aact:AA01906|5end_repressor_protein_CI_1286305..1286535_POSITIVE 321 129 85 191 125 aact:AA00579|5end_probable_DNA_recombination_protein_395265..395495_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 128 82 149 101 aact:AA01467|3end_mannose-specific_phosphotransferase_system_IID_987214..987364_POSITIVE 371 130 83 63 3 aact:AA01585|3end_tRNA_delta(2)-isopentenylpyrophosphate_1067073..1067223_POSITIVE 368 118 72 127 86 aact:AA02687|3end_hydrogenase-4_component_D_1874043..1874193_POSITIVE 365 130 82 116 50 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 364 117 71 151 107 aact:AA00987|3end_tetracenomycin_polyketide_synthesis_O-methyltransferase_690586..690736_POSITIVE 339 130 88 61 25 aact:AA00576|3end_conserved_hypothetical_protein_(possible_translation_factor)_394648..394798_POSITIVE 336 129 82 146 101 aact:AA02077|3end_probable_outer_membrane_protein_1412708..1412858_POSITIVE 330 117 71 138 98 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 329 120 72 73 21 aact:AA01829|3end_hypothetical_protein_1231662..1231812_POSITIVE 324 128 87 103 62 aact:AA01989|3end_hypothetical_protein_1352241..1352391_POSITIVE 323 127 84 54 6 aact:AA01424|3end_threonine_deaminase_(dehydratase)_958233..958383_POSITIVE 323 123 82 133 95 aact:AA01368|3end_50S_ribosomal_protein_L19_924912..925062_POSITIVE 319 130 81 66 4 aact:AA01001|3end_conserved_hypothetical_protein_699974..700124_POSITIVE 317 128 82 71 19 aact:AA01359|3end_conserved_hypothetical_protein_921109..921259_POSITIVE 317 130 82 66 16 aact:AA00530|3end_hypothetical_protein_362668..362818_POSITIVE 315 113 72 71 27 aact:AA02560|3end_integral_membrane_protein_1783630..1783780_POSITIVE 315 117 71 150 85 aact:AA01423|3end_cell_division_protein_X,_ABC_system_permease_957680..957830_POSITIVE 314 109 65 96 38 aact:AA02674|3end_formate_dehydrogenase-related_protein_1860732..1860882_POSITIVE 313 129 97 43 6 aact:AA01789|3end_protease_DegS_precursor;_periplasmic_serine_endoprotease_1207002..1207152_POSITIVE 313 130 81 65 10 aact:AA00538|3end_hypothetical_protein_369111..369261_POSITIVE