Origin IGS: tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagccgaagtagtcataccaagcctccttcctatttttttgctccctccgtttacggggggagctgccgaaggctgagggggggattttgcccccttccgtccttcggacaccttcccccgcaagcaggggaaggcaaaaatttttcta Mask Tandem Repeat Region ================================================ tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Find is-nt database================================================ Query_seq: AA02947:AA02949|AA02947:AA02949:riboflavin biosynthesis protein:hypothetical protein:->->:2061935..2062083 149 tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02947:AA02949|AA02947:AA02949:riboflavin biosynthesis protein:hypothetical protein:->->:2061935..2062083 149 tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02947:AA02949|AA02947:AA02949:riboflavin biosynthesis protein:hypothetical protein:->->:2061935..2062083 149 tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 22 End: 147 ..................... M L A G E G V R R T E G G K I P P S A F G S S P R K R R E Q K N R K E A W Y D Y F G .. Protein_Len: 47 Strand: + Start: 8 End: 148 ....... M F A F P C L R G K V S E G R K G A K S P P Q P S A A P P V N G G S K K I G R R L G M T T S A . tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02947:AA02949|AA02947:AA02949:riboflavin biosynthesis protein:hypothetical protein:->->:2061935..2062083 149 tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Intra-Species Hit: Count: 1 Min: 1 Max: 149 Len: 149 Subject: aact_AA02947_AA02949|riboflavin biosynthesis protein:hypothetical protein|POSITIVE:POSITIVE|[2061935,2062083]|149 HSP 1 e-value: 2.0E-54 bit: 212.0 Len: 149 Query Start:1 Query End:149 Subject Strand: POSITIVE Subject Start: 1 Subject End: 149 tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatnnnnnnntcagccttcggcagctccccccgtaaacggagggagcnnnnnnntaggaaggaggcttggtatgactacttcggcta tagaaaaatttttgccttcccctgcttgcgggggaaggtgtccgaaggacggaagggggcaaaatccccccctcagccttcggcagctccccccgtaaacggagggagcaaaaaaataggaaggaggcttggtatgactacttcggcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAAAAUUUUUGCCUUCCCCUGCUUGCGGGGGAAGGUGUCCGAAGGACGGAAGGGGGCAAAAUCCCCCCCUCAGCCUUCGGCAGCUCCCCCCGUAAACGGAGGGAGCAAAAAAAUAGGAAGGAGGCUUGGUAUGACUACUUCGGCUA ........(((((((..(((((((.(((((((((.((.((.(((((((...((.(((((.......))))).))..))))))))).)).))))))))).))).)))))))))))...........((((.((((....))))..)))). (-69.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCCGAAGUAGUCAUACCAAGCCUCCUUCCUAUUUUUUUGCUCCCUCCGUUUACGGGGGGAGCUGCCGAAGGCUGAGGGGGGGAUUUUGCCCCCUUCCGUCCUUCGGACACCUUCCCCCGCAAGCAGGGGAAGGCAAAAAUUUUUCUA .....((((.((.(.......).)).)))).....((((((((..((((((((.(((((((((.((((((((((.((((((((.......)))))))).).))))))).)).))))))))).)))).))))..))))))))........ (-69.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 498 60 15 183 141 aact:AA01379|5end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_933029..933259_POSITIVE 463 59 16 164 123 aact:AA01422|5end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956009..956239_POSITIVE 419 68 21 111 46 aact:AA01473|5end_type_I_restriction-modification_system_S_(specificity_subunit)_989025..989255_POSITIVE 418 59 11 181 115 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 407 60 14 218 175 aact:AA00161|5end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_113384..113614_POSITIVE 406 53 11 57 15 aact:AA01642|5end_possible_iron(III)_ABC_transporter_1110970..1111200_POSITIVE 402 60 11 107 28 aact:AA01546|5end_deoxyribose_operon_repressor_1035494..1035724_POSITIVE 399 90 46 230 171 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 390 44 11 135 95 aact:AA01891|5end_pyridoxine_kinase_1275922..1276152_POSITIVE 390 59 17 204 161 aact:AA00862|5end_conserved_hypothetical_protein_596465..596695_POSITIVE 380 51 16 146 102 aact:AA01695|5end_carbohydrate_transport_protein_1150445..1150675_POSITIVE 377 60 12 119 60 aact:AA00142|5end_glycerate_kinase_101309..101539_POSITIVE 376 59 15 198 144 aact:AA00366|5end_50S_ribosomal_protein_L2_246215..246445_POSITIVE 375 69 21 129 66 aact:AA01877|5end_conserved_hypothetical_protein_1264115..1264345_POSITIVE 372 45 5 205 164 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 370 107 64 77 20 aact:AA01845|5end_gamma-glutamyltranspeptidase_precursor_1239841..1240071_POSITIVE 367 70 24 187 128 aact:AA01730|5end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_1168725..1168955_POSITIVE 365 75 32 87 51 aact:AA02632|5end_rhamnosyltransferase_1831850..1832080_POSITIVE 365 69 21 192 150 aact:AA00199|5end_hypothetical_protein_138575..138805_POSITIVE 360 107 62 224 167 aact:AA00979|5end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_684645..684875_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 496 110 68 78 32 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 414 70 22 80 15 aact:AA01675|3end_probable_multidrug_resistance_protein;_multidrug-efflux_transporter_1133589..1133739_POSITIVE 414 60 14 85 23 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 406 53 11 43 1 aact:AA01641|3end_hypothetical_protein_1111036..1111186_POSITIVE 402 60 20 142 92 aact:AA00212|3end_ribokinase_147047..147197_POSITIVE 399 90 46 135 76 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 390 44 11 100 60 aact:AA01890|3end_cell-cycle_protein_1275967..1276117_POSITIVE 380 51 16 88 44 aact:AA01694|3end_PTS_system,_IIA_component_1150467..1150617_POSITIVE 375 69 21 65 2 aact:AA01876|3end_PTS_system,_IIBC_components_1264131..1264281_POSITIVE 373 49 10 79 48 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 367 49 15 41 13 aact:AA01421|3end_phosphoglycolate_phosphatase_955806..955956_POSITIVE 365 75 32 121 85 aact:AA02631|3end_undecaprenyl-phosphate_galactosephosphotransferase_homolog_1831964..1832114_POSITIVE 360 107 62 84 27 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 359 59 15 148 99 aact:AA01472|3end_hypothetical_protein_989158..989308_POSITIVE 357 108 61 84 15 aact:AA01873|3end_mannose-6-phosphate_isomerase_1261940..1262090_POSITIVE 348 67 23 149 99 aact:AA01467|3end_mannose-specific_phosphotransferase_system_IID_987214..987364_POSITIVE 340 60 14 102 56 aact:AA02601|3end_ATP-dependent_helicase_1813309..1813459_POSITIVE 339 70 26 81 36 aact:AA02179|3end_anthanilate_phosphoribosyltransferase_1490445..1490595_POSITIVE 337 68 21 59 2 aact:AA00820|3end_succinyl-diaminopimelate_desuccinylase_564536..564686_POSITIVE 329 83 47 106 61 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE