Origin IGS: attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagtaagattaaagtgcggttggaaaaaatatcgtttttttgaatattatctggaatattttttatagagatagcatgttaaattacctttttacagatcgtaataattatcaaatcacatttcccctttcagatttttaat Mask Tandem Repeat Region ================================================ attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Find is-nt database================================================ Query_seq: AA02929:AA02930|AA02929:AA02930:hypothetical protein:fructose-1,6-bisphosphatase:->->:2049431..2049573 143 attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02929:AA02930|AA02929:AA02930:hypothetical protein:fructose-1,6-bisphosphatase:->->:2049431..2049573 143 attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02929:AA02930|AA02929:AA02930:hypothetical protein:fructose-1,6-bisphosphatase:->->:2049431..2049573 143 attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 5 End: 112 .... * F D S L P F T I Q Y N N R D T F L Y N L M S D R Y F I N W I I N L F V M ............................... attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02929:AA02930|AA02929:AA02930:hypothetical protein:fructose-1,6-bisphosphatase:->->:2049431..2049573 143 attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Intra-Species Hit: Count: 1 Min: 1 Max: 143 Len: 143 Subject: aact_AA02929_AA02930|hypothetical protein:fructose-1, 6-bisphosphatase|POSITIVE:POSITIVE|[2049431,2049573]|143 HSP 1 e-value: 3.0E-63 bit: 242.0 Len: 143 Query Start:1 Query End:143 Subject Strand: POSITIVE Subject Start: 1 Subject End: 143 attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcnnnnnnncgatattttttccaaccgcactttaatcttactta attaaaaatctgaaaggggaaatgtgatttgataattattacgatctgtaaaaaggtaatttaacatgctatctctataaaaaatattccagataatattcaaaaaaacgatattttttccaaccgcactttaatcttactta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAAAAAUCUGAAAGGGGAAAUGUGAUUUGAUAAUUAUUACGAUCUGUAAAAAGGUAAUUUAACAUGCUAUCUCUAUAAAAAAUAUUCCAGAUAAUAUUCAAAAAAACGAUAUUUUUUCCAACCGCACUUUAAUCUUACUUA ...............((..((((((..(((((...........((((......))))............(((((..(((.....)))...)))))....)))))...)))....)))..))...................... (-11.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUAAGAUUAAAGUGCGGUUGGAAAAAAUAUCGUUUUUUUGAAUAUUAUCUGGAAUAUUUUUUAUAGAGAUAGCAUGUUAAAUUACCUUUUUACAGAUCGUAAUAAUUAUCAAAUCACAUUUCCCCUUUCAGAUUUUUAAU .......(((.....(((((((.(((((..((((........(((((((......)))))))........))))....((......)).)))))...)))))))......))).............................. (-14.29) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 132 100 115 84 aact:AA02703|5end_hypothetical_protein_1885365..1885595_POSITIVE 243 129 103 231 205 aact:AA00533|5end_conserved_hypothetical_protein_365323..365553_POSITIVE 239 133 105 214 186 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 236 132 97 132 97 aact:AA01554|5end_conserved_hypothetical_protein;_hypothetical_membrane_protein_1042061..1042291_POSITIVE 236 137 104 126 93 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 236 140 103 140 106 aact:AA00091|5end_short-patch_reverse_transcription_protein_67741..67971_POSITIVE 235 137 104 163 130 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 235 132 103 179 150 aact:AA02101|5end_penicillin-binding_protein_7_1430830..1431060_POSITIVE 234 135 101 173 140 aact:AA02486|5end_topoisomerase_IV,_subunit_B_1724337..1724567_POSITIVE 233 132 103 94 65 aact:AA00233|5end_hypothetical_protein_162339..162569_POSITIVE 230 130 84 123 69 aact:AA00134|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_(menaquinone_biosynthetic_protein)_95317..95547_POSITIVE 228 138 100 159 123 aact:AA02113|5end_conserved_hypothetical_protein_1442183..1442413_POSITIVE 228 138 99 212 175 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 226 56 14 66 21 aact:AA02523|5end_conserved_hypothetical_protein_1752990..1753220_POSITIVE 225 132 103 226 196 aact:AA02776|5end_conserved_hypothetical_protein_1939693..1939923_POSITIVE 220 140 114 158 132 aact:AA02872|5end_conserved_hypothetical_protein_2015539..2015769_POSITIVE 216 132 100 194 161 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 216 130 87 172 124 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 215 139 99 150 112 aact:AA02449|5end_hypothetical_protein_1687575..1687805_POSITIVE 214 132 102 162 132 aact:AA02128|5end_DNA_topoisomerase_I_1451395..1451625_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 132 100 55 24 aact:AA02702|3end_succinyl-CoA_synthetase,_beta_chain_1885385..1885535_POSITIVE 236 137 104 65 32 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 236 140 103 68 34 aact:AA00089|3end_S-adenosylmethionine_synthase;_methionine_adenosyltransferase_67749..67899_POSITIVE 233 132 103 74 45 aact:AA00231|3end_long-chain-fatty-acid--CoA_ligase_162399..162549_POSITIVE 228 138 100 80 44 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 224 137 119 32 15 aact:AA00809|3end_possible_oxidoreductase_555435..555585_POSITIVE 220 140 114 91 65 aact:AA02870|3end_conserved_hypothetical_protein_2015552..2015702_POSITIVE 217 132 105 28 1 aact:AA02100|3end_lipoprotein_1430759..1430909_POSITIVE 216 132 100 81 48 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 215 139 99 78 40 aact:AA02446|3end_conserved_hypothetical_protein_1687583..1687733_POSITIVE 214 132 102 71 41 aact:AA02126|3end_conserved_hypothetical_protein_1451384..1451534_POSITIVE 214 135 99 147 109 aact:AA00507|3end_hypothetical_protein_353201..353351_POSITIVE 214 132 92 132 94 aact:AA00500|3end_hypothetical_protein_347356..347506_POSITIVE 214 132 92 55 17 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE 206 132 106 59 33 aact:AA00234|3end_collagen_adhesin_168641..168791_POSITIVE 205 134 103 75 44 aact:AA01880|3end_N-acetylglucosamine_repressor_1268383..1268533_POSITIVE 205 132 103 112 83 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 204 132 103 112 83 aact:AA02621|3end_hypothetical_protein_1827288..1827438_POSITIVE 204 132 103 36 7 aact:AA02620|3end_orf14;_possible_membrane_protein_1827212..1827362_POSITIVE 204 140 109 83 59 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE