Origin IGS: ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaaacaaataggagagaaaatgtccgtgaatttaattgaaatgggagaacaggcaaaacaagccgctttcccaactttctcaacgggaaaaaaatcaagccttgacaatcattgccgagtaattggag Mask Tandem Repeat Region ================================================ ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Find is-nt database================================================ Query_seq: AA02922:AA02925|AA02922:AA02925:gamma-glutamylphosphate reductase:conserved hypothetical protein:->->:2047502..2047632 131 ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02922:AA02925|AA02922:AA02925:gamma-glutamylphosphate reductase:conserved hypothetical protein:->->:2047502..2047632 131 ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02922:AA02925|AA02922:AA02925:gamma-glutamylphosphate reductase:conserved hypothetical protein:->->:2047502..2047632 131 ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 31 End: 129 .............................. M I F F P L R K L G K R L V L P V L P F Q L N S R T F S L L F V F .. Protein_Len: 37 Strand: + Start: 21 End: 131 .................... M S R L D F F P V E K V G K A A C F A C S P I S I K F T D I F S P I C F L Protein_Len: 40 Strand: - Start: 2 End: 121 . G I V R C H N D L S S K K G T S F T P F A A Q K A Q E G M E I L N V S M K E G M .......... ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 53 HP_score: -3.0 Tail_Score: -3.78409 Start: 56 End: 87 Full_Region: TCCCGTTGAGAAAGT TGGGAAAGCGGC TTGTTTT GCCTGTTCTCCCA TTTCAATTAAATTCA .......................................................TGGGAAAGCGGCTTGTTTTGCCTGTTCTCCCA............................................ Find igs database================================================ Query_seq: AA02922:AA02925|AA02922:AA02925:gamma-glutamylphosphate reductase:conserved hypothetical protein:->->:2047502..2047632 131 ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Intra-Species Hit: Count: 1 Min: 1 Max: 131 Len: 131 Subject: aact_AA02922_AA02925|gamma-glutamylphosphate reductase:conserved hypothetical protein|POSITIVE:POSITIVE|[2047502,2047632]|131 HSP 1 e-value: 3.0E-56 bit: 218.0 Len: 131 Query Start:1 Query End:131 Subject Strand: POSITIVE Subject Start: 1 Subject End: 131 ctccaattactcggcaatgattgtcaaggcttgannnnnnncccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta ctccaattactcggcaatgattgtcaaggcttgatttttttcccgttgagaaagttgggaaagcggcttgttttgcctgttctcccatttcaattaaattcacggacattttctctcctatttgtttttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCCAAUUACUCGGCAAUGAUUGUCAAGGCUUGAUUUUUUUCCCGUUGAGAAAGUUGGGAAAGCGGCUUGUUUUGCCUGUUCUCCCAUUUCAAUUAAAUUCACGGACAUUUUCUCUCCUAUUUGUUUUUUA .(((.....((((((...((..((((.....))))..)).....)))))).....(((((.((((((.......))).))).)))))................)))......................... (-25.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAACAAAUAGGAGAGAAAAUGUCCGUGAAUUUAAUUGAAAUGGGAGAACAGGCAAAACAAGCCGCUUUCCCAACUUUCUCAACGGGAAAAAAAUCAAGCCUUGACAAUCAUUGCCGAGUAAUUGGAG .......(((...(((.(.....).)))..........((((..(((((((...(((.......)))..)))))))..(((((.....)))))....))))...)))..........((((....)))).. (-27.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 325 83 42 147 105 aact:AA00492|5end_conserved_hypothetical_protein_343578..343808_POSITIVE 317 59 12 79 30 aact:AA00832|5end_D-alanine-D-alanine_ligase_B_572769..572999_POSITIVE 317 80 43 187 146 aact:AA00259|5end_conserved_hypothetical_protein_182788..183018_POSITIVE 315 108 63 190 142 aact:AA02696|5end_formate_hydrogenlyase_maturation_protein_1879476..1879706_POSITIVE 315 69 21 72 13 aact:AA00066|5end_NADP-dependent_malic_enzyme_(NADP-ME)_49002..49232_POSITIVE 309 87 42 104 41 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 296 70 27 228 181 aact:AA02146|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1468828..1469058_POSITIVE 292 77 34 204 159 aact:AA00864|5end_conserved_hypothetical_protein_598605..598835_POSITIVE 291 83 42 62 24 aact:AA01368|5end_50S_ribosomal_protein_L19_924485..924715_POSITIVE 290 90 41 152 93 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 289 80 40 177 147 aact:AA02736|5end_conserved_hypothetical_protein_1917350..1917580_POSITIVE 289 105 65 208 165 aact:AA02368|5end_conserved_hypothetical_protein_1623544..1623774_POSITIVE 288 69 25 213 162 aact:AA01590|5end_hypothetical_protein_1070766..1070996_POSITIVE 288 59 12 224 180 aact:AA00202|5end_conserved_hypothetical_protein_(possible_membrane_protein)_140546..140776_POSITIVE 285 78 33 208 158 aact:AA02119|5end_hypothetical_protein_1446372..1446602_POSITIVE 284 89 43 71 28 aact:AA01631|5end_dihydrodipicolinate_reductase_1103026..1103256_POSITIVE 284 65 21 226 179 aact:AA00738|5end_hypothetical_protein_505087..505317_POSITIVE 282 85 42 103 42 aact:AA00343|5end_conserved_hypothetical_protein_(possible_cytosolic_protein)_229908..230138_POSITIVE 279 86 48 87 38 aact:AA01832|5end_formate_dehydrogenase_formation_protein_E_1233609..1233839_POSITIVE 277 55 11 132 91 aact:AA02581|5end_conserved_hypothetical_protein_1796966..1797196_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 351 89 42 143 92 aact:AA01901|3end_hypothetical_protein_1282870..1283020_POSITIVE 323 89 41 58 5 aact:AA02944|3end_30S_ribosomal_protein_S20_2059028..2059178_POSITIVE 317 59 12 79 30 aact:AA00831|3end_cell_division_protein_Q_572849..572999_POSITIVE 315 108 63 52 4 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 315 69 21 76 17 aact:AA00065|3end_hypothetical_protein_49086..49236_POSITIVE 309 87 42 98 35 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE 297 85 46 113 67 aact:AA02727|3end_hypothetical_protein_1909925..1910075_POSITIVE 293 79 46 103 61 aact:AA01041|3end_hypothetical_protein_725055..725205_POSITIVE 284 89 43 46 3 aact:AA01630|3end_threonine_efflux_protein_1103081..1103231_POSITIVE 283 80 40 123 67 aact:AA02837|3end_conserved_hypothetical_protein_1992977..1993127_POSITIVE 277 55 11 132 91 aact:AA02579|3end_aminotransferase_nifS_protein_1797046..1797196_POSITIVE 276 80 35 83 36 aact:AA00708|3end_cysteine_desulfurase_487395..487545_POSITIVE 274 81 43 68 35 aact:AA01069|3end_translation_elongation_factor_(EF-Ts)_740700..740850_POSITIVE 272 47 2 111 51 aact:AA00085|3end_menaquinone-specific_isochorismate;_isochorismate_mutase_63523..63673_POSITIVE 269 87 45 86 43 aact:AA02609|3end_ABC_transporter,_ATP-binding_protein/permease_1820184..1820334_POSITIVE 268 90 44 62 13 aact:AA00960|3end_possible_outer_membrane_protein_667228..667378_POSITIVE 267 86 41 53 3 aact:AA01756|3end_cobalt_membrane_transport_protein_1187217..1187367_POSITIVE 266 76 41 123 82 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 264 79 37 79 43 aact:AA01725|3end_hypothetical_protein_1168182..1168332_POSITIVE 264 79 35 108 72 aact:AA01174|3end_hypothetical_protein_804923..805073_POSITIVE