Origin IGS: aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagcttgtgtaatcaattcgttataaaatttccttttatcaagggggagtttatgccagaatgaagcacattttttataaaaagtgcggtataaaaatatgttgtttcgtggaggattt Mask Tandem Repeat Region ================================================ aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Find is-nt database================================================ Query_seq: AA02917:AA02918|AA02917:AA02918:cystathionine gamma-synthase:hypothetical protein:->->:2043341..2043459 119 aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02917:AA02918|AA02917:AA02918:cystathionine gamma-synthase:hypothetical protein:->->:2043341..2043459 119 aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02917:AA02918|AA02917:AA02918:cystathionine gamma-synthase:hypothetical protein:->->:2043341..2043459 119 aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 3 End: 104 .. M L H E T T Y F Y T A L F I K N V L H S G I N S P L I K G N F I T N ............... Protein_Len: 33 Strand: + Start: 20 End: 118 ................... M F L Y R T F Y K K C A S F W H K L P L D K R K F Y N E L I T Q A . Protein_Len: 34 Strand: - Start: 3 End: 104 .. D E V F C C I K I G C K K Y F I H K M R A Y V G G Q Y F S I K Y R M ............... aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02917:AA02918|AA02917:AA02918:cystathionine gamma-synthase:hypothetical protein:->->:2043341..2043459 119 aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Intra-Species Hit: Count: 1 Min: 1 Max: 119 Len: 119 Subject: aact_AA02917_AA02918|cystathionine gamma-synthase:hypothetical protein|POSITIVE:POSITIVE|[2043341,2043459]|119 HSP 1 e-value: 1.0E-61 bit: 236.0 Len: 119 Query Start:1 Query End:119 Subject Strand: POSITIVE Subject Start: 1 Subject End: 119 aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta aaatcctccacgaaacaacatatttttataccgcactttttataaaaaatgtgcttcattctggcataaactcccccttgataaaaggaaattttataacgaattgattacacaagcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUCCUCCACGAAACAACAUAUUUUUAUACCGCACUUUUUAUAAAAAAUGUGCUUCAUUCUGGCAUAAACUCCCCCUUGAUAAAAGGAAAUUUUAUAACGAAUUGAUUACACAAGCUA .................................((.(((((((((((..((((((.......)))))).......((((.....))))...)))))))).))).))............. (-13.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCUUGUGUAAUCAAUUCGUUAUAAAAUUUCCUUUUAUCAAGGGGGAGUUUAUGCCAGAAUGAAGCACAUUUUUUAUAAAAAGUGCGGUAUAAAAAUAUGUUGUUUCGUGGAGGAUUU ((((..((.......))..))))..(((((((((((.....)))))))))))...((...(((((((((((((((((((..........)))))))).))).))))))))...)).... (-22.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 300 75 31 153 111 aact:AA01209|5end_conserved_hypothetical_protein_826430..826660_POSITIVE 265 66 32 159 121 aact:AA01070|5end_DNA_repair_protein_740920..741150_POSITIVE 250 89 54 116 75 aact:AA02675|5end_formate_dehydrogenase_alpha_subunit_1861011..1861241_POSITIVE 248 76 31 159 114 aact:AA00809|5end_possible_oxidoreductase_553211..553441_POSITIVE 245 65 22 165 119 aact:AA02999|5end_conserved_hypothetical_protein_2094275..2094505_POSITIVE 241 79 31 200 142 aact:AA01640|5end_restriction_enzyme_type_I_helicase_subunit_1109230..1109460_POSITIVE 241 79 31 106 48 aact:AA01639|5end_hypothetical_protein_1109136..1109366_POSITIVE 241 39 3 102 62 aact:AA00915|5end_xanthine-guanine_phosphoribosyltransferase_629610..629840_POSITIVE 240 76 31 133 86 aact:AA00733|5end_hypothetical_protein_502335..502565_POSITIVE 239 74 31 176 135 aact:AA01248|5end_conserved_hypothetical_protein_(possible_phosphoesterase)_849734..849964_POSITIVE 239 74 31 106 65 aact:AA01247|5end_hypothetical_protein_849664..849894_POSITIVE 238 54 11 219 170 aact:AA00990|5end_conserved_hypothetical_protein_692559..692789_POSITIVE 237 80 31 178 132 aact:AA01530|5end_conserved_hypothetical_protein_1027308..1027538_POSITIVE 235 64 21 154 105 aact:AA00134|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_(menaquinone_biosynthetic_protein)_95317..95547_POSITIVE 233 68 23 168 124 aact:AA03010|5end_thiophene_and_furan_oxidation_protein_2101102..2101332_POSITIVE 233 68 23 67 23 aact:AA03009|5end_hypothetical_protein_2101001..2101231_POSITIVE 232 79 31 97 53 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 229 60 17 150 104 aact:AA00254|5end_penicillin-binding_protein_5_177579..177809_POSITIVE 228 80 31 167 117 aact:AA00109|5end_conserved_hypothetical_protein_78582..78812_POSITIVE 226 60 11 140 100 aact:AA02836|5end_colicin_transport_protein_1991777..1992007_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 300 75 31 106 64 aact:AA01207|3end_hypothetical_protein_826463..826613_POSITIVE 248 76 31 56 11 aact:AA00807|3end_fumarate_reductase_13_kD_hydrophobic_protein_553188..553338_POSITIVE 240 75 31 123 81 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 240 76 31 145 98 aact:AA00732|3end_citrate_lyase_ligase_502427..502577_POSITIVE 239 74 31 43 2 aact:AA01245|3end_tyrosine-specific_transport_protein_2_849681..849831_POSITIVE 237 80 31 50 4 aact:AA01528|3end_probable_oxidoreductase_1027260..1027410_POSITIVE 235 64 21 56 7 aact:AA00132|3end_conserved_hypothetical_protein_(possible_regulator_protein,_VirR_family)_95299..95449_POSITIVE 233 68 23 58 14 aact:AA03008|3end_GTP-binding_protein_era_homolog_(GTPase)_2101072..2101222_POSITIVE 232 79 31 125 81 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 229 97 63 92 56 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 229 60 17 127 81 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 227 67 30 140 102 aact:AA00108|3end_conserved_hypothetical_protein_78648..78798_POSITIVE 226 60 11 51 11 aact:AA02834|3end_colicin_transport_protein_1991768..1991918_POSITIVE 224 58 11 86 38 aact:AA02587|3end_conserved_hypothetical_protein_1800570..1800720_POSITIVE 223 39 3 127 97 aact:AA01247|3end_hypothetical_protein_849944..850094_POSITIVE 222 78 31 64 17 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 220 58 22 150 114 aact:AA02998|3end_conserved_hypothetical_protein_2094350..2094500_POSITIVE 219 57 11 96 33 aact:AA00488|3end_purine_nucleoside_phosphorylase_338871..339021_POSITIVE 218 66 29 36 2 aact:AA02909|3end_conserved_hypothetical_protein_2035813..2035963_POSITIVE 217 91 51 146 94 aact:AA01249|3end_conserved_hypothetical_protein_(possible_phosphoesterase)_850423..850573_POSITIVE