Origin IGS: tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatgcagcattcggcataagacattgataggtcaatgattttttagcgtcaaaatgaacgtcggcttgcaggccattttttctaacaggttttgttttattaaaatttcgacattcgattgattttaataggtagtca Mask Tandem Repeat Region ================================================ tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Find is-nt database================================================ Query_seq: AA02911:AA02912|AA02911:AA02912:hypothetical protein:hypothetical protein:->->:2039296..2039434 139 tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02911:AA02912|AA02911:AA02912:hypothetical protein:hypothetical protein:->->:2039296..2039434 139 tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02911:AA02912|AA02911:AA02912:hypothetical protein:hypothetical protein:->->:2039296..2039434 139 tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 20 End: 139 ................... M E C R N F N K T K P V R K N G L Q A D V H F D A K K S L T Y Q C L M P N A A L tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 33 End: 62 ................................TTTTTCTAACAGGTTTTGTTTTATTAAAAT............................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02911:AA02912|AA02911:AA02912:hypothetical protein:hypothetical protein:->->:2039296..2039434 139 tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Intra-Species Hit: Count: 1 Min: 1 Max: 139 Len: 139 Subject: aact_AA02911_AA02912|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2039296,2039434]|139 HSP 1 e-value: 2.0E-73 bit: 276.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta tgactacctattaaaatcaatcgaatgtcgaaattttaataaaacaaaacctgttagaaaaaatggcctgcaagccgacgttcattttgacgctaaaaaatcattgacctatcaatgtcttatgccgaatgctgcatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACUACCUAUUAAAAUCAAUCGAAUGUCGAAAUUUUAAUAAAACAAAACCUGUUAGAAAAAAUGGCCUGCAAGCCGACGUUCAUUUUGACGCUAAAAAAUCAUUGACCUAUCAAUGUCUUAUGCCGAAUGCUGCAUUA ................((((..(((((((....((((((((..........)))))))).....(((......))))))))))...))))...........((((((....))))))....((((.(....).)))).. (-21.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGCAGCAUUCGGCAUAAGACAUUGAUAGGUCAAUGAUUUUUUAGCGUCAAAAUGAACGUCGGCUUGCAGGCCAUUUUUUCUAACAGGUUUUGUUUUAUUAAAAUUUCGACAUUCGAUUGAUUUUAAUAGGUAGUCA .............(((.((((((.(((.((((..............(((((.....).)))).((((....))))......)))).))))))))).(((((((((((((((.....)))..))))))))))))..))). (-25.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 110 64 222 160 aact:AA02267|5end_conserved_hypothetical_protein_1545416..1545646_POSITIVE 305 97 61 52 11 aact:AA01151|5end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_788323..788553_POSITIVE 297 80 50 227 199 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 295 93 64 42 1 aact:AA00452|5end_phosphomannomutase;_phosphoglucomutase_315288..315518_POSITIVE 287 93 65 209 178 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 285 106 61 189 134 aact:AA02934|5end_hypothetical_protein_2054242..2054472_POSITIVE 282 119 74 101 47 aact:AA01767|5end_conserved_hypothetical_protein_1192528..1192758_POSITIVE 282 110 70 178 130 aact:AA01340|5end_possible_lipoprotein;_possible_HlyD_family_secretion_domain_protein;_possible_membrane_fusion_protein_905746..905976_POSITIVE 280 106 65 223 186 aact:AA01880|5end_N-acetylglucosamine_repressor_1267083..1267313_POSITIVE 276 117 71 134 70 aact:AA00596|5end_uridylate_kinase_407496..407726_POSITIVE 275 89 52 167 124 aact:AA00235|5end_hypothetical_protein_168596..168826_POSITIVE 274 109 66 228 171 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 273 119 75 135 80 aact:AA00882|5end_hypothetical_protein_613452..613682_POSITIVE 272 93 66 56 35 aact:AA01069|5end_translation_elongation_factor_(EF-Ts)_739775..740005_POSITIVE 270 95 51 211 156 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 266 128 82 102 61 aact:AA00666|5end_hypothetical_protein_457470..457700_POSITIVE 263 93 68 114 81 aact:AA02712|5end_tail_specific_protease_precursor_1892853..1893083_POSITIVE 263 97 64 177 140 aact:AA01630|5end_threonine_efflux_protein_1102381..1102611_POSITIVE 263 95 51 62 22 aact:AA01423|5end_cell_division_protein_X,_ABC_system_permease_956668..956898_POSITIVE 262 94 51 209 173 aact:AA02270|5end_endopeptidase_La;_lon_protease;_protease_La_homolog;_ATP-dependent_protease_LA_(lon-1)_1546870..1547100_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 110 64 148 86 aact:AA02266|3end_exodeoxyribonuclease_V_beta_chain_1545422..1545572_POSITIVE 297 80 50 123 95 aact:AA00196|3end_conserved_hypothetical_protein_138015..138165_POSITIVE 282 119 74 129 75 aact:AA01765|3end_Mu-like_prophage_FluMu_transposase_A_1192636..1192786_POSITIVE 282 110 70 149 101 aact:AA01339|3end_transcriptional_regulator,_AcrR/TetR_family_905797..905947_POSITIVE 275 89 52 132 89 aact:AA00234|3end_collagen_adhesin_168641..168791_POSITIVE 273 119 75 88 33 aact:AA00881|3end_hypothetical_protein_613485..613635_POSITIVE 272 93 66 51 30 aact:AA01068|3end_hypothetical_protein_739850..740000_POSITIVE 266 128 82 102 61 aact:AA00665|3end_plasmid_protein_457550..457700_POSITIVE 263 95 51 50 10 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 262 94 51 63 27 aact:AA02269|3end_3-hydroxydecanoyl-(acyl-carrier_protein)_dehydratase_1546804..1546954_POSITIVE 258 93 51 151 117 aact:AA01146|3end_conserved_hypothetical_protein_784770..784920_POSITIVE 249 94 57 147 116 aact:AA01879|3end_glutamyl-tRNA_reductase_1267098..1267248_POSITIVE 247 110 66 101 60 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE 245 93 53 142 96 aact:AA00117|3end_hypothetical_protein_88418..88568_POSITIVE 244 93 54 147 101 aact:AA01476|3end_hypothetical_protein_992261..992411_POSITIVE 242 90 42 62 9 aact:AA01369|3end_rod_shape-determining_protein_925513..925663_POSITIVE 242 89 45 69 29 aact:AA00344|3end_preprotein_translocase_secE_subunit_231505..231655_POSITIVE 241 127 85 78 36 aact:AA01954|3end_excinuclease_ABC,_subunit_C_1322314..1322464_POSITIVE 241 96 51 91 25 aact:AA01876|3end_PTS_system,_IIBC_components_1264131..1264281_POSITIVE 239 95 63 129 91 aact:AA02236|3end_stringent_starvation_protein_A_1522637..1522787_POSITIVE