Origin IGS: tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaatttcgcctctaaataaggaccgatttcctgtgtaatgtgtttgagctcccttggctttccagtaatgcaagaaaattacgtatttcatcatgtaacccgttttaccaataatcggcgcta Mask Tandem Repeat Region ================================================ tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Find is-nt database================================================ Query_seq: AA02889:AA02890|AA02889:AA02890:hypothetical protein:hypothetical protein:->->:2025332..2025457 126 tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02889:AA02890|AA02889:AA02890:hypothetical protein:hypothetical protein:->->:2025332..2025457 126 tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02889:AA02890|AA02889:AA02890:hypothetical protein:hypothetical protein:->->:2025332..2025457 126 tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 9 End: 125 ........ M I G K T G Y M M K Y V I F L H Y W K A K G A Q T H Y T G N R S L F R G E I F . Protein_Len: 32 Strand: - Start: 13 End: 108 ............ Q Y F P N C S S I R L K R A N S S L W P L E F V N C L F D T R M .................. Protein_Len: 32 Strand: - Start: 3 End: 98 .. R R N N T F R T V H H F V Y N E Q M V P F G L S S L C M V C S M ............................ tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02889:AA02890|AA02889:AA02890:hypothetical protein:hypothetical protein:->->:2025332..2025457 126 tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Intra-Species Hit: Count: 1 Min: 1 Max: 126 Len: 126 Subject: aact_AA02889_AA02890|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2025332,2025457]|126 HSP 1 e-value: 9.0E-66 bit: 250.0 Len: 126 Query Start:1 Query End:126 Subject Strand: POSITIVE Subject Start: 1 Subject End: 126 tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta tagcgccgattattggtaaaacgggttacatgatgaaatacgtaattttcttgcattactggaaagccaagggagctcaaacacattacacaggaaatcggtccttatttagaggcgaaattttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCGCCGAUUAUUGGUAAAACGGGUUACAUGAUGAAAUACGUAAUUUUCUUGCAUUACUGGAAAGCCAAGGGAGCUCAAACACAUUACACAGGAAAUCGGUCCUUAUUUAGAGGCGAAAUUUUUA ....(((((((.(((((((..(.(((...(((..((((........))))...))).))).)..((((....).)))........)))).)))..)))))))((((.....))))........... (-21.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAUUUCGCCUCUAAAUAAGGACCGAUUUCCUGUGUAAUGUGUUUGAGCUCCCUUGGCUUUCCAGUAAUGCAAGAAAAUUACGUAUUUCAUCAUGUAACCCGUUUUACCAAUAAUCGGCGCUA .........((((........((((......))))(((.((((.(((.(((((.....)))))....(((((........)))))..............))).)))).)))........))))... (-18.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 117 71 135 83 aact:AA01195|5end_acyl_carrier_protein_819849..820079_POSITIVE 276 100 51 90 31 aact:AA02793|5end_inositol-1-monophosphatase;_extragenic_suppressor_protein_1960978..1961208_POSITIVE 276 118 71 156 92 aact:AA00099|5end_conserved_hypothetical_protein_72621..72851_POSITIVE 274 115 75 87 37 aact:AA02710|5end_conserved_hypothetical_protein_1890642..1890872_POSITIVE 270 49 3 43 4 aact:AA01177|5end_beta-ketoacyl-ACP_synthase_IV_806732..806962_POSITIVE 268 103 61 69 32 aact:AA01115|5end_carboxylesterase_766545..766775_POSITIVE 268 99 54 56 5 aact:AA00614|5end_ribonuclease_HII_(RNase_HII)_419890..420120_POSITIVE 267 117 71 108 47 aact:AA01763|5end_conserved_hypothetical_protein_(possible_transcriptional_regulatory_protein)_1190643..1190873_POSITIVE 266 117 71 150 104 aact:AA00633|5end_hypothetical_protein_434971..435201_POSITIVE 258 70 22 53 5 aact:AA01878|5end_hydroxyacylglutathione_hydrolase_1264831..1265061_POSITIVE 256 50 3 153 101 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 255 46 3 87 42 aact:AA01519.1|5end_conserved_hypothetical_protein_1022466..1022696_POSITIVE 255 46 3 48 3 aact:AA01520|5end_hypothetical_protein_1022427..1022657_POSITIVE 255 104 66 172 128 aact:AA01306|5end_glutathione_reductase_886538..886768_POSITIVE 253 28 2 218 193 aact:AA01198|5end_hypothetical_protein_821636..821866_POSITIVE 250 49 3 61 14 aact:AA01499|5end_fructose_1,6-bisphosphatase_protein_1008357..1008587_POSITIVE 246 117 71 49 2 aact:AA01327|5end_possible_LOS_biosynthesis_enzyme_(D-glycero-D-manno-heptosyl_transferase)_895568..895798_POSITIVE 244 119 75 217 168 aact:AA02731|5end_outer_membrane_lipoprotein_carrier_precursor_1911498..1911728_POSITIVE 243 100 55 88 22 aact:AA00461|5end_heat_shock_protein;_protease_322375..322605_POSITIVE 243 76 39 183 150 aact:AA00086|5end_2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy_late_synthase_63437..63667_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 117 71 129 77 aact:AA01194|3end_acyl_carrier_protein_819923..820073_POSITIVE 277 70 23 134 82 aact:AA00504|3end_ATPase_351287..351437_POSITIVE 276 100 51 69 10 aact:AA02792|3end_GlpT/UhpT_family_transporter_(hexosphosphate_transport)_1961037..1961187_POSITIVE 268 99 54 60 9 aact:AA00613|3end_lipid-A-disaccharide_synthase_419974..420124_POSITIVE 259 108 66 74 18 aact:AA01272|3end_hypothetical_protein_864211..864361_POSITIVE 255 46 3 80 35 aact:AA01519|3end_conserved_hypothetical_protein_1022539..1022689_POSITIVE 255 104 66 74 30 aact:AA01305|3end_conserved_hypothetical_protein_886520..886670_POSITIVE 247 118 71 86 49 aact:AA01989|3end_hypothetical_protein_1352241..1352391_POSITIVE 242 72 33 149 110 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 236 31 3 42 17 aact:AA01705|3end_beta-galactosidase_1157980..1158130_POSITIVE 234 50 4 111 71 aact:AA02237|3end_molybdopterin_biosynthesis_protein_E_chain_1523292..1523442_POSITIVE 231 118 71 42 8 aact:AA02281|3end_phosphoglycerate_transport_system_activator_protein_1553474..1553624_POSITIVE 229 104 67 114 74 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 228 105 61 136 93 aact:AA01673|3end_riboflavin_synthase,_alpha_chain_1132093..1132243_POSITIVE 228 45 5 130 100 aact:AA01510|3end_ABC_transporter,_ATP-binding_protein/permease_1018165..1018315_POSITIVE 227 35 3 84 55 aact:AA00382|3end_hypothetical_protein_258885..259035_POSITIVE 224 94 54 97 39 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 224 85 41 76 29 aact:AA00883|3end_hypothetical_protein_614467..614617_POSITIVE 223 103 62 62 6 aact:AA01523|3end_conserved_hypothetical_protein_1023569..1023719_POSITIVE 222 45 2 103 50 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE