Origin IGS: aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggactaaataatggagcaaggttactgaaaataccgggctacgtaattggggtctaactagtgtgcattttctagtcttactaactgcaaatcccgaattaattttcgggattt Mask Tandem Repeat Region ================================================ aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Find is-nt database================================================ Query_seq: AA02876:AA02878|AA02876:AA02878:conserved hypothetical protein:conserved hypothetical protein:->->:2018814..2018929 116 aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02876:AA02878|AA02876:AA02878:conserved hypothetical protein:conserved hypothetical protein:->->:2018814..2018929 116 aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02876:AA02878|AA02876:AA02878:conserved hypothetical protein:conserved hypothetical protein:->->:2018814..2018929 116 aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 3 End: 116 .. M P K I N S G F A V S K T R K C T L V R P Q L R S P V F S V T L L H Y L V L aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02876:AA02878|AA02876:AA02878:conserved hypothetical protein:conserved hypothetical protein:->->:2018814..2018929 116 aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Intra-Species Hit: Count: 1 Min: 1 Max: 116 Len: 116 Subject: aact_AA02876_AA02878|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2018814,2018929]|116 HSP 1 e-value: 8.0E-60 bit: 230.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta aaatcccgaaaattaattcgggatttgcagttagtaagactagaaaatgcacactagttagaccccaattacgtagcccggtattttcagtaaccttgctccattatttagtccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUCCCGAAAAUUAAUUCGGGAUUUGCAGUUAGUAAGACUAGAAAAUGCACACUAGUUAGACCCCAAUUACGUAGCCCGGUAUUUUCAGUAACCUUGCUCCAUUAUUUAGUCCUA ((((((((((......))))))))))......(((((((((.((((((((...(((((............)).)))....)))))))))))...))))))................ (-26.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGACUAAAUAAUGGAGCAAGGUUACUGAAAAUACCGGGCUACGUAAUUGGGGUCUAACUAGUGUGCAUUUUCUAGUCUUACUAACUGCAAAUCCCGAAUUAAUUUUCGGGAUUU ((((((((...((((.(((..(((..........)))..))).....(((((.......)))))...))))...))))))))........((((((((((......)))))))))) (-31.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 337 103 72 205 176 aact:AA02368|5end_conserved_hypothetical_protein_1623544..1623774_POSITIVE 318 90 42 66 11 aact:AA02961|5end_pyrroline-5-carboxylate_reductase_2070785..2071015_POSITIVE 305 99 52 229 175 aact:AA01338|5end_hypothetical_protein_904967..905197_POSITIVE 286 88 47 138 96 aact:AA01355|5end_large_subunit_pseudouridylate_synthase_D_917228..917458_POSITIVE 284 100 52 60 20 aact:AA01473|5end_type_I_restriction-modification_system_S_(specificity_subunit)_989025..989255_POSITIVE 280 85 42 230 185 aact:AA02545|5end_conserved_hypothetical_protein_1773965..1774195_POSITIVE 274 106 63 54 24 aact:AA02250|5end_possible_cytochrome_c-type_protein_1530125..1530355_POSITIVE 259 99 57 93 49 aact:AA02586|5end_lipid_A_biosynthesis_lauroyl_acyltransferase_1799207..1799437_POSITIVE 251 99 56 180 133 aact:AA01340|5end_possible_lipoprotein;_possible_HlyD_family_secretion_domain_protein;_possible_membrane_fusion_protein_905746..905976_POSITIVE 245 86 44 188 145 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 243 104 65 107 61 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 242 100 51 74 19 aact:AA01386|5end_30S_ribosomal_protein_S1_935974..936204_POSITIVE 241 105 65 48 3 aact:AA01509|5end_ABC_system_transport_protein,_ATP_binding/permease_1014692..1014922_POSITIVE 237 100 57 175 138 aact:AA02354|5end_conserved_hypothetical_protein_1612236..1612466_POSITIVE 237 99 51 80 20 aact:AA01301|5end_competence_protein_B_881467..881697_POSITIVE 237 98 51 80 28 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 236 109 69 89 38 aact:AA02081|5end_periplasmic_protein_1414698..1414928_POSITIVE 235 100 53 126 74 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 234 50 11 45 6 aact:AA02635|5end_conserved_hypothetical_protein_1833678..1833908_POSITIVE 234 92 63 41 12 aact:AA02146|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1468828..1469058_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 288 99 52 146 100 aact:AA02544|3end_ATP-dependent_RNA_helicase_1773945..1774095_POSITIVE 286 88 47 151 109 aact:AA01354|3end_conserved_hypothetical_protein_917321..917471_POSITIVE 284 100 52 113 73 aact:AA01472|3end_hypothetical_protein_989158..989308_POSITIVE 282 69 21 118 67 aact:AA02585|3end_hypothetical_protein_1799093..1799243_POSITIVE 268 27 1 91 65 aact:AA02876|3end_conserved_hypothetical_protein_2018753..2018903_POSITIVE 251 99 56 151 104 aact:AA01339|3end_transcriptional_regulator,_AcrR/TetR_family_905797..905947_POSITIVE 239 100 54 55 3 aact:AA01385|3end_50S_ribosomal_protein_L9_936035..936185_POSITIVE 237 100 57 124 87 aact:AA02353|3end_spermidine_/_putrescine_transport_ATP-binding_1612265..1612415_POSITIVE 237 98 51 73 21 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 236 109 69 105 54 aact:AA02080|3end_ABC_transporter,_ATP-binding/permease_1414794..1414944_POSITIVE 235 100 53 53 1 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 234 87 44 149 96 aact:AA02656|3end_possible_polysaccharide_polymerization_protein_1851980..1852130_POSITIVE 234 50 11 49 10 aact:AA02634|3end_rhamnosyltransferase;_glycosyltransferase_homolog_2_1833762..1833912_POSITIVE 231 78 34 147 103 aact:AA01889|3end_conserved_hypothetical_protein_(possible_permease)_1274590..1274740_POSITIVE 226 79 37 100 47 aact:AA00172|3end_conserved_hypothetical_protein_(possible_regulator_protein_/_nitrogen_fixation_protein)_122904..123054_POSITIVE 224 102 62 121 85 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 222 99 64 80 46 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 221 86 42 105 66 aact:AA01308|3end_cyclic_AMP_receptor_protein_888732..888882_POSITIVE 220 105 63 85 47 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 218 102 71 85 56 aact:AA01952|3end_ABC_transporter,_substrate_binding_protein_1320155..1320305_POSITIVE