Origin IGS: tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gagtaaattatcggtttttcgtttttgttgataacgctacaacacccgatcatcggcaatattttcctactgccgaagtacagagcgtatgatctcaattaagactttca Mask Tandem Repeat Region ================================================ tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Find is-nt database================================================ Query_seq: AA02874:AA02875|AA02874:AA02875:hypothetical protein:conserved hypothetical protein (2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase):->->:2017960..2018069 110 tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02874:AA02875|AA02874:AA02875:hypothetical protein:conserved hypothetical protein (2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase):->->:2017960..2018069 110 tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02874:AA02875|AA02874:AA02875:hypothetical protein:conserved hypothetical protein (2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase):->->:2017960..2018069 110 tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 13 End: 108 ............ M R S Y A L Y F G S R K I L P M I G C C S V I N K N E K P I I Y .. Protein_Len: 31 Strand: - Start: 12 End: 104 ........... N L D Y A R Y K P L L F I N G I I P H Q L T I L L F S F G I M ...... tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02874:AA02875|AA02874:AA02875:hypothetical protein:conserved hypothetical protein (2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase):->->:2017960..2018069 110 tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Intra-Species Hit: Count: 1 Min: 1 Max: 110 Len: 110 Subject: aact_AA02874_AA02875|hypothetical protein:conserved hypothetical protein (2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase)|POSITIVE:POSITIVE|[2017960,2018069]|110 HSP 1 e-value: 3.0E-56 bit: 218.0 Len: 110 Query Start:1 Query End:110 Subject Strand: POSITIVE Subject Start: 1 Subject End: 110 tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc tgaaagtcttaattgagatcatacgctctgtacttcggcagtaggaaaatattgccgatgatcgggtgttgtagcgttatcaacaaaaacgaaaaaccgataatttactc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAGUCUUAAUUGAGAUCAUACGCUCUGUACUUCGGCAGUAGGAAAAUAUUGCCGAUGAUCGGGUGUUGUAGCGUUAUCAACAAAAACGAAAAACCGAUAAUUUACUC .....(((((....)))))...((((((.((...(((((((((......)))))))))..)).)))))).((((.((((((.................)))))))))).. (-26.03) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGUAAAUUAUCGGUUUUUCGUUUUUGUUGAUAACGCUACAACACCCGAUCAUCGGCAAUAUUUUCCUACUGCCGAAGUACAGAGCGUAUGAUCUCAAUUAAGACUUUCA ((((.((((...((((...(((((((((((.........)))))........((((((.((......)).)))))).....))))))...))))..))))...))))... (-18.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 367 76 36 212 168 aact:AA00937|5end_L-xylulokinase_646816..647046_POSITIVE 346 80 33 230 174 aact:AA01247|5end_hypothetical_protein_849664..849894_POSITIVE 332 67 24 130 92 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 327 67 22 170 129 aact:AA00365|5end_30S_ribosomal_protein_S19_245914..246144_POSITIVE 324 68 23 208 156 aact:AA01588|5end_N-acetylmuramoyl-L-alanine_amidase_II_1068816..1069046_POSITIVE 317 80 31 83 39 aact:AA02481|5end_conserved_hypothetical_protein_1719085..1719315_POSITIVE 308 76 31 215 172 aact:AA02968|5end_hypothetical_protein_2075917..2076147_POSITIVE 307 78 33 64 22 aact:AA02676|5end_formate_dehydrogenase_alpha_subunit_1861671..1861901_POSITIVE 307 76 33 98 53 aact:AA02553|5end_acylphosphatase_1779321..1779551_POSITIVE 304 59 14 67 28 aact:AA00899|5end_possible_quaternary_ammonium_compound_resistance_protein_623214..623444_POSITIVE 302 78 34 48 5 aact:AA02438|5end_membrane_protein;_possible_ABC_transporter_permease_protein_1682590..1682820_POSITIVE 301 68 27 71 29 aact:AA02565|5end_transketolase,_N-terminal_subunit_1786366..1786596_POSITIVE 301 80 33 82 22 aact:AA02028|5end_conserved_hypothetical_protein_1381058..1381288_POSITIVE 294 80 37 63 28 aact:AA00995|5end_sensory_transduction_protein_for_cpxR/basR_696355..696585_POSITIVE 293 75 34 81 39 aact:AA01876|5end_PTS_system,_IIBC_components_1263365..1263595_POSITIVE 293 80 33 136 101 aact:AA01428|5end_acetolactate_synthase_958688..958918_POSITIVE 293 68 24 220 180 aact:AA01210|5end_hypothetical_protein_826903..827133_POSITIVE 292 77 36 45 9 aact:AA01654|5end_periplasmic_maltose-binding_protein_precursor_1119896..1120126_POSITIVE 291 82 44 90 49 aact:AA01448|5end_hypothetical_protein_970208..970438_POSITIVE 291 75 33 227 189 aact:AA00649|5end_peptide_chain_release_factor_1_(RF-1)_443277..443507_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 352 75 34 149 101 aact:AA01423|3end_cell_division_protein_X,_ABC_system_permease_957680..957830_POSITIVE 314 80 35 136 85 aact:AA02410|3end_cell_division_protein_1656543..1656693_POSITIVE 312 69 23 68 22 aact:AA02874|3end_hypothetical_protein_2017899..2018049_POSITIVE 308 76 31 132 89 aact:AA02967|3end_elongation_factor_P_(EF-P)_2075914..2076064_POSITIVE 307 76 33 117 72 aact:AA02552|3end_iron_(chelated)_ABC_transporter,_permease_protein_1779420..1779570_POSITIVE 293 68 24 99 61 aact:AA02601|3end_ATP-dependent_helicase_1813309..1813459_POSITIVE 293 75 34 142 100 aact:AA01875|3end_PTS_system,_glucose-specific_enzyme_II,_A_component_1263506..1263656_POSITIVE 292 77 36 73 37 aact:AA01651|3end_hypothetical_protein_1120004..1120154_POSITIVE 291 82 44 128 87 aact:AA01446|3end_conserved_hypothetical_protein_970326..970476_POSITIVE 291 55 11 144 99 aact:AA00363|3end_50S_ribosomal_protein_L22_245980..246130_POSITIVE 289 71 32 141 93 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 287 78 36 66 23 aact:AA02379|3end_hypothetical_protein_1628123..1628273_POSITIVE 286 86 52 147 114 aact:AA00398|3end_protein-tyrosine-phosphatase_(PTPase)_268018..268168_POSITIVE 283 80 40 146 103 aact:AA02810|3end_glycine_hydroxymethyltransferase;_serine_hydroxymethyltransferase_1979524..1979674_POSITIVE 283 70 24 150 81 aact:AA01992|3end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1355004..1355154_POSITIVE 283 68 33 151 111 aact:AA01245|3end_tyrosine-specific_transport_protein_2_849681..849831_POSITIVE 280 78 36 42 1 aact:AA02437|3end_integral_membrane_protein_1682664..1682814_POSITIVE 277 70 24 58 15 aact:AA01369|3end_rod_shape-determining_protein_925513..925663_POSITIVE 275 70 24 129 64 aact:AA01394|3end_transcriptional_regulator_(mtr_efflux_pump)_943211..943361_POSITIVE 275 80 33 71 30 aact:AA01184|3end_hypothetical_protein_811133..811283_POSITIVE