Origin IGS: ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggtaaaattctgaagaaaatctgctttttaactagccagaaaaagtgcaagtctgtagaatagccagtcaatttttatgaaccattagagtaaattttagccaatatgacgcaaaaactacatattaaaacctggggctgccag Mask Tandem Repeat Region ================================================ ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Find is-nt database================================================ Query_seq: AA02873:AA02874|AA02873:AA02874:conserved hypothetical protein:hypothetical protein:->->:2017688..2017833 146 ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02873:AA02874|AA02873:AA02874:conserved hypothetical protein:hypothetical protein:->->:2017688..2017833 146 ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02873:AA02874|AA02873:AA02874:conserved hypothetical protein:hypothetical protein:->->:2017688..2017833 146 ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Predict ORF larger than 30AA ================================================ ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02873:AA02874|AA02873:AA02874:conserved hypothetical protein:hypothetical protein:->->:2017688..2017833 146 ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Intra-Species Hit: Count: 1 Min: 1 Max: 146 Len: 146 Subject: aact_AA02873_AA02874|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2017688,2017833]|146 HSP 1 e-value: 1.0E-77 bit: 289.0 Len: 146 Query Start:1 Query End:146 Subject Strand: POSITIVE Subject Start: 1 Subject End: 146 ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta ctggcagccccaggttttaatatgtagtttttgcgtcatattggctaaaatttactctaatggttcataaaaattgactggctattctacagacttgcactttttctggctagttaaaaagcagattttcttcagaattttaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUGGCAGCCCCAGGUUUUAAUAUGUAGUUUUUGCGUCAUAUUGGCUAAAAUUUACUCUAAUGGUUCAUAAAAAUUGACUGGCUAUUCUACAGACUUGCACUUUUUCUGGCUAGUUAAAAAGCAGAUUUUCUUCAGAAUUUUACCUA ((((.....)))).....((((((..((....))..))))))((.((((((((....................(((((((((((......................)))))))))))......((......)).)))))))))).. (-23.35) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUAAAAUUCUGAAGAAAAUCUGCUUUUUAACUAGCCAGAAAAAGUGCAAGUCUGUAGAAUAGCCAGUCAAUUUUUAUGAACCAUUAGAGUAAAUUUUAGCCAAUAUGACGCAAAAACUACAUAUUAAAACCUGGGGCUGCCAG ..((((..((((((.(((......(((((((..........))))))).....))).))))))..((((...(((((.(((...))).)))))...........((((((............)))))).....))))...)))).. (-21.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 282 110 61 172 121 aact:AA01232|5end_30S_ribosomal_protein_S8_840445..840675_POSITIVE 281 46 1 221 156 aact:AA01878|5end_hydroxyacylglutathione_hydrolase_1264831..1265061_POSITIVE 275 50 2 56 14 aact:AA01339|5end_transcriptional_regulator,_AcrR/TetR_family_905154..905384_POSITIVE 269 115 78 209 163 aact:AA01868|5end_biotin_synthetase_1255545..1255775_POSITIVE 263 110 68 154 118 aact:AA02649|5end_possible_glycosyltransferase_1845562..1845792_POSITIVE 256 46 1 147 105 aact:AA02637|5end_conserved_hypothetical_protein_(possible_membrane_protein)_1835142..1835372_POSITIVE 255 46 2 142 91 aact:AA00735|5end_citrate_lyase,_beta_chain_502674..502904_POSITIVE 249 44 1 178 137 aact:AA00413|5end_conserved_hypothetical_protein_280142..280372_POSITIVE 249 45 1 118 64 aact:AA00099|5end_conserved_hypothetical_protein_72621..72851_POSITIVE 246 45 5 55 6 aact:AA00281|5end_phosphatidylserine_decarboxylase_proenzyme_197939..198169_POSITIVE 245 66 24 128 79 aact:AA02514|5end_esterase/lipase_1747501..1747731_POSITIVE 245 46 2 98 59 aact:AA02492|5end_glutathione_S-transferase_1729366..1729596_POSITIVE 243 46 1 183 133 aact:AA00348|5end_50S_ribosomal_protein_L1_232570..232800_POSITIVE 242 46 18 221 194 aact:AA01877|5end_conserved_hypothetical_protein_1264115..1264345_POSITIVE 240 107 63 218 179 aact:AA02570|5end_conserved_hypothetical_protein_1789150..1789380_POSITIVE 240 107 63 80 41 aact:AA02569|5end_hypothetical_protein_1789012..1789242_POSITIVE 238 45 2 97 58 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 238 46 1 91 50 aact:AA01020|5end_enonolase;_2-phosphoglycerate_dehydratase_712741..712971_POSITIVE 237 45 2 225 173 aact:AA00803|5end_hypothetical_protein_549526..549756_POSITIVE 236 135 92 170 125 aact:AA02999|5end_conserved_hypothetical_protein_2094275..2094505_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 282 110 61 132 81 aact:AA01231|3end_30S_ribosomal_protein_S14_840485..840635_POSITIVE 263 110 68 149 113 aact:AA02648|3end_lytic_transglycosylase_1845637..1845787_POSITIVE 255 46 2 142 91 aact:AA00734|3end_citrate_lyase,_gamma_chain_(acyl_carrier_protein)_502754..502904_POSITIVE 240 45 3 63 9 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 238 45 2 86 47 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 235 128 81 62 10 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 234 46 3 110 57 aact:AA00795|3end_iron(III)_dicitrate-binding_periplasmic_protein_precursor_545763..545913_POSITIVE 233 46 2 101 50 aact:AA01947|3end_iron(III)-transport_ATP-binding_protein_1317330..1317480_POSITIVE 233 45 2 151 111 aact:AA00735|3end_citrate_lyase,_beta_chain_503626..503776_POSITIVE 230 35 1 55 10 aact:AA02931|3end_UDP-N-acetylmuramate:L-alanyl-gamma-d-glutamayl-m_edo-diaminopimelate_2052046..2052196_POSITIVE 229 45 1 113 53 aact:AA01579|3end_transcriptional_regulatory_protein_1065283..1065433_POSITIVE 229 111 75 133 94 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 229 45 5 144 101 aact:AA00419|3end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284911..285061_POSITIVE 228 45 2 142 102 aact:AA02146|3end_iron(III)_ABC_transporter,_ATP-binding_protein_1469477..1469627_POSITIVE 226 86 42 131 85 aact:AA02139|3end_possible_membrane_protein_1462218..1462368_POSITIVE 222 114 79 103 65 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 219 45 3 150 97 aact:AA01220|3end_competence_protein_F;_amidophosphoribosyltransferase_833538..833688_POSITIVE 219 46 1 139 102 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 219 50 2 138 95 aact:AA00246|3end_hypothetical_protein_173340..173490_POSITIVE 218 45 1 108 47 aact:AA02477|3end_PTS_system,_N-acetylglucosamine-permease_IIABC_component_1713371..1713521_POSITIVE