Origin IGS: ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttgtttatccttatgagttaagttgttgataatttactagaaaaataaaatttgtctaatgaaatcacgattttgggttttcggttcattgggctgaaaagaaaacccctcgaaagttaacttccgaggggttgtgataaaccatgaattattcgcaccttgctcggaagtgatcttccaagcacaccacatacctgaaagattattcagttacatgatggtgatgatgg Mask Tandem Repeat Region ================================================ ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Find is-nt database================================================ Query_seq: AA02869:AA02870|AA02869:AA02870:hypothetical protein:conserved hypothetical protein:->->:2014033..2014262 230 ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02869:AA02870|AA02869:AA02870:hypothetical protein:conserved hypothetical protein:->->:2014033..2014262 230 ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02869:AA02870|AA02869:AA02870:hypothetical protein:conserved hypothetical protein:->->:2014033..2014262 230 ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Predict ORF larger than 30AA ================================================ Protein_Len: 57 Strand: + Start: 21 End: 191 .................... M N N L S G M W C A W K I T S E Q G A N N S W F I T T P R K L T F E G F S F Q P N E P K T Q N R D F I R Q I L F F ....................................... Protein_Len: 32 Strand: - Start: 2 End: 97 . M M V M M Y S F L R E P I H H A Q F I V E S C P A F L E H N I M ..................................................................................................................................... ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 46 HP_score: -3.3 Tail_Score: -3.10894 Start: 21 End: 36 Full_Region: aagcacaccacatac ctgaa agatta ttcag ttacatgatggtgat ....................ctgaaagattattcag.................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -15.5 Tail_Score: -5.07652 Start: 100 End: 125 Full_Region: CATGGTTTATCACAA CCCCTCGGAAG TTAA CTTTCGAGGGG TTTTCTTTTCAGCCC ...................................................................................................CCCCTCGGAAGTTAACTTTCGAGGGG......................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -13.3 Tail_Score: -3.4072 Start: 100 End: 125 Full_Region: gggctgaaaagaaaa cccctcgaaag ttaa cttccgagggg ttgtgataaaccatg ...................................................................................................cccctcgaaagttaacttccgagggg......................................................................................................... Find igs database================================================ Query_seq: AA02869:AA02870|AA02869:AA02870:hypothetical protein:conserved hypothetical protein:->->:2014033..2014262 230 ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Intra-Species Hit: Count: 1 Min: 1 Max: 230 Len: 230 Subject: aact_AA02869_AA02870|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2014033,2014262]|230 HSP 1 e-value: 1.0E-127 bit: 456.0 Len: 230 Query Start:1 Query End:230 Subject Strand: POSITIVE Subject Start: 1 Subject End: 230 ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa ccatcatcaccatcatgtaactgaataatctttcaggtatgtggtgtgcttggaagatcacttccgagcaaggtgcgaataattcatggtttatcacaacccctcggaagttaactttcgaggggttttcttttcagcccaatgaaccgaaaacccaaaatcgtgatttcattagacaaattttatttttctagtaaattatcaacaacttaactcataaggataaacaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCAUCAUCACCAUCAUGUAACUGAAUAAUCUUUCAGGUAUGUGGUGUGCUUGGAAGAUCACUUCCGAGCAAGGUGCGAAUAAUUCAUGGUUUAUCACAACCCCUCGGAAGUUAACUUUCGAGGGGUUUUCUUUUCAGCCCAAUGAACCGAAAACCCAAAAUCGUGAUUUCAUUAGACAAAUUUUAUUUUUCUAGUAAAUUAUCAACAACUUAACUCAUAAGGAUAAACAA .((((.....(((((((((.(((((......))))).))))))))).(((((((((....)))))))))..)))).............(((((((...((((((((((((....))))))))))))((((..((((......))))..))))..........(((((((.((((((.((((...)))).)))))))))))))...................))))))).. (-69.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGUUUAUCCUUAUGAGUUAAGUUGUUGAUAAUUUACUAGAAAAAUAAAAUUUGUCUAAUGAAAUCACGAUUUUGGGUUUUCGGUUCAUUGGGCUGAAAAGAAAACCCCUCGAAAGUUAACUUCCGAGGGGUUGUGAUAAACCAUGAAUUAUUCGCACCUUGCUCGGAAGUGAUCUUCCAAGCACACCACAUACCUGAAAGAUUAUUCAGUUACAUGAUGGUGAUGAUGG ......((((((.(((((.(((.(((.(((((((((.((((.((((...)))).)))).....(((((.........(((((((((.....)))))))))...(((((((((.(((....))).))))))))))))))......))))))))).))).)))))))).))).)))...(((..((((((((((..(((((......)))))....))).))))).)).))) (-65.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 390 77 33 106 61 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 357 109 61 91 35 aact:AA01062|5end_ribonuclease_PH_(RNase_PH)_735706..735936_POSITIVE 354 78 34 50 5 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 334 77 41 203 162 aact:AA00578|5end_DNA_topoisomerase_394576..394806_POSITIVE 332 80 40 208 169 aact:AA02832|5end_outer_membrane_integrity_protein_1990044..1990274_POSITIVE 331 110 62 111 59 aact:AA02140|5end_ABC_transporter_ATP-binding_protein_1462144..1462374_POSITIVE 330 157 117 219 180 aact:AA02134|5end_aminopeptidase_N_1456181..1456411_POSITIVE 323 78 37 217 169 aact:AA01165|5end_conserved_hypothetical_protein_798133..798363_POSITIVE 321 77 34 108 68 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 313 77 33 202 162 aact:AA00270|5end_malonyl_coA-acyl_carrier_protein_transacylase_189361..189591_POSITIVE 312 75 39 158 112 aact:AA01878|5end_hydroxyacylglutathione_hydrolase_1264831..1265061_POSITIVE 312 72 35 74 46 aact:AA00846|5end_cell_division_protein_L_584643..584873_POSITIVE 305 120 72 218 169 aact:AA01360|5end_XAA-pro_aminopeptidase_(X-pro_aminopeptidase)_921047..921277_POSITIVE 305 77 36 101 55 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 304 78 33 99 45 aact:AA01495|5end_probable_oxidoreductase_1004092..1004322_POSITIVE 303 76 34 214 174 aact:AA01551|5end_hypothetical_protein_1038190..1038420_POSITIVE 303 76 34 60 20 aact:AA01550|5end_hypothetical_protein_1038036..1038266_POSITIVE 301 77 39 159 114 aact:AA00558|5end_UDP-n-acetylenolpyruvoylglucosamine_reductase_382126..382356_POSITIVE 299 82 41 94 56 aact:AA00390|5end_permease_263689..263919_POSITIVE 295 80 33 192 153 aact:AA00184|5end_hypothetical_protein_130682..130912_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 390 77 33 95 50 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 361 76 35 137 90 aact:AA02623|3end_dTDP-D-glucose_4,6-dehydratase_1827979..1828129_POSITIVE 357 109 61 78 22 aact:AA01059|3end_orotate_phosphoribosyltransferase_735773..735923_POSITIVE 331 110 62 105 53 aact:AA02139|3end_possible_membrane_protein_1462218..1462368_POSITIVE 330 157 117 143 104 aact:AA02132|3end_dihydroorotate_dehydrogenase_1456185..1456335_POSITIVE 312 75 39 137 91 aact:AA01877|3end_conserved_hypothetical_protein_1264890..1265040_POSITIVE 312 72 35 81 53 aact:AA00845|3end_hypothetical_protein_584730..584880_POSITIVE 307 77 37 151 104 aact:AA01164|3end_conserved_hypothetical_protein_(possible_integral_membrane_protein)_798148..798298_POSITIVE 302 77 35 97 50 aact:AA01875|3end_PTS_system,_glucose-specific_enzyme_II,_A_component_1263506..1263656_POSITIVE 301 77 39 141 96 aact:AA00557|3end_nitrate/nitrite_sensor_protein_382188..382338_POSITIVE 287 69 21 79 31 aact:AA01660|3end_hypothetical_protein_1125282..1125432_POSITIVE 285 77 40 140 103 aact:AA01543|3end_hypothetical_protein_1034350..1034500_POSITIVE 284 90 42 111 54 aact:AA01416|3end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_953336..953486_POSITIVE 284 88 42 93 41 aact:AA01187|3end_conserved_hypothetical_protein_813992..814142_POSITIVE 279 79 31 130 77 aact:AA02823|3end_exodeoxyribonuclease_V_gamma_chain_1986169..1986319_POSITIVE 277 80 32 92 38 aact:AA02242|3end_molybdenum_cofactor_biosynthesis_protein_A_1525088..1525238_POSITIVE 276 70 47 44 21 aact:AA02869|3end_hypothetical_protein_2013972..2014122_POSITIVE 276 109 65 83 44 aact:AA01192|3end_conserved_hypothetical_protein_(possible_membrane_protein)_817977..818127_POSITIVE 276 80 35 108 51 aact:AA00223|3end_catalase_(hydroperoxidase;_iron_heme_axial_ligand)_155808..155958_POSITIVE 275 76 33 94 56 aact:AA00286|3end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200260..200410_POSITIVE