Origin IGS: taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtattctccctcacataactaaaaactgcgccaattatgccacagaatgagggcatttgtttggagaaaataaaaaggactaagcagcttaggcttaatcctttttgaatatgacgcatcatagaaacacggtgaaaacccaccgcacttttatggctta Mask Tandem Repeat Region ================================================ taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Find is-nt database================================================ Query_seq: AA02865:AA02866|AA02865:AA02866:iron-regulated outer membrane protein (omp64):lactoylglutathione lyase:->->:2012603..2012763 161 taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02865:AA02866|AA02865:AA02866:iron-regulated outer membrane protein (omp64):lactoylglutathione lyase:->->:2012603..2012763 161 taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02865:AA02866|AA02865:AA02866:iron-regulated outer membrane protein (omp64):lactoylglutathione lyase:->->:2012603..2012763 161 taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 18 End: 161 ................. M G F H R V S M M R H I Q K G L S L S C L V L F I F S K Q M P S F C G I I G A V F S Y V R E N T taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 95 HP_score: -6.5 Tail_Score: -5.54907 Start: 60 End: 84 Full_Region: CGTCATATTCAAAAA GGATTAAGC CTAAGCT GCTTAGTCC TTTTTATTTTCTCCA ...........................................................GGATTAAGCCTAAGCTGCTTAGTCC............................................................................. TransTerm Strand: - Conf: 72 HP_score: -4.3 Tail_Score: -4.70659 Start: 60 End: 84 Full_Region: tggagaaaataaaaa ggactaagc agcttag gcttaatcc tttttgaatatgacg ...........................................................ggactaagcagcttaggcttaatcc............................................................................. TransTerm Strand: + Conf: 71 HP_score: -4.2 Tail_Score: -4.96646 Start: 116 End: 135 Full_Region: ACAAATGCCCTCATT CTGTGGCA TAAT TGGCGCAG TTTTTAGTTATGTGA ...................................................................................................................CTGTGGCATAATTGGCGCAG.......................... Find igs database================================================ Query_seq: AA02865:AA02866|AA02865:AA02866:iron-regulated outer membrane protein (omp64):lactoylglutathione lyase:->->:2012603..2012763 161 taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Intra-Species Hit: Count: 1 Min: 1 Max: 161 Len: 161 Subject: aact_AA02865_AA02866|iron-regulated outer membrane protein (omp64):lactoylglutathione lyase|POSITIVE:POSITIVE|[2012603,2012763]|161 HSP 1 e-value: 2.0E-86 bit: 319.0 Len: 161 Query Start:1 Query End:161 Subject Strand: POSITIVE Subject Start: 1 Subject End: 161 taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc taagccataaaagtgcggtgggttttcaccgtgtttctatgatgcgtcatattcaaaaaggattaagcctaagctgcttagtcctttttattttctccaaacaaatgccctcattctgtggcataattggcgcagtttttagttatgtgagggagaatacc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCCAUAAAAGUGCGGUGGGUUUUCACCGUGUUUCUAUGAUGCGUCAUAUUCAAAAAGGAUUAAGCCUAAGCUGCUUAGUCCUUUUUAUUUUCUCCAAACAAAUGCCCUCAUUCUGUGGCAUAAUUGGCGCAGUUUUUAGUUAUGUGAGGGAGAAUACC ...((((((.(((..((((((....))))))..))).))))..))....(((((.(((((((((((((.......)))))))))))))...................(((((((.(((((.((....)).)))))...........))))))).))))).. (-52.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUAUUCUCCCUCACAUAACUAAAAACUGCGCCAAUUAUGCCACAGAAUGAGGGCAUUUGUUUGGAGAAAAUAAAAAGGACUAAGCAGCUUAGGCUUAAUCCUUUUUGAAUAUGACGCAUCAUAGAAACACGGUGAAAACCCACCGCACUUUUAUGGCUUA ((......)).................((((((((..(((((.(.......))))))....))))......(((((((((.(((((.......))))).))))))))).......)))).(((((((...(((((......)))))...)))))))..... (-40.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 422 41 6 126 90 aact:AA02231|5end_probable_dethiobiotin_synthetase_1_1517650..1517880_POSITIVE 415 48 1 182 131 aact:AA01584|5end_host_factor-I_protein_1065589..1065819_POSITIVE 401 36 5 133 100 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 400 47 10 66 15 aact:AA00143|5end_gluconate_permease_(GntP_family)_102445..102675_POSITIVE 397 48 4 105 62 aact:AA00384|5end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_260108..260338_POSITIVE 383 36 5 109 77 aact:AA02832|5end_outer_membrane_integrity_protein_1990044..1990274_POSITIVE 383 47 7 189 149 aact:AA01020|5end_enonolase;_2-phosphoglycerate_dehydratase_712741..712971_POSITIVE 375 46 10 176 140 aact:AA02381|5end_4-hydroxy-2-oxoglutarate_aldolase_1629192..1629422_POSITIVE 374 36 4 59 28 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 364 41 9 142 108 aact:AA02792|5end_GlpT/UhpT_family_transporter_(hexosphosphate_transport)_1959617..1959847_POSITIVE 360 47 4 91 50 aact:AA00139|5end_antibiotic_maturation_factor_99098..99328_POSITIVE 358 48 9 226 183 aact:AA02774|5end_collagenase_prtC_homolog,_possible_protease_1936870..1937100_POSITIVE 358 48 9 55 12 aact:AA02773|5end_hypothetical_protein_1936699..1936929_POSITIVE 352 46 6 82 42 aact:AA00595|5end_hypothetical_protein_407194..407424_POSITIVE 349 46 2 170 131 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 349 48 8 137 92 aact:AA00186|5end_o-succinylbenzoate-CoA_synthase;_OSB_synthase_131367..131597_POSITIVE 348 48 3 79 31 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 346 50 11 92 46 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 343 47 1 214 170 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 340 46 9 198 165 aact:AA02257|5end_conserved_hypothetical_protein_1536518..1536748_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 401 36 5 80 47 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 400 47 10 66 15 aact:AA00142|3end_glycerate_kinase_102525..102675_POSITIVE 397 48 4 99 56 aact:AA00383|3end_chloride_channel_protein_260182..260332_POSITIVE 383 36 5 71 39 aact:AA02830|3end_colicin_tolerance_protein_1990086..1990236_POSITIVE 383 47 7 87 47 aact:AA01019|3end_conserved_hypothetical_protein_712719..712869_POSITIVE 375 46 10 84 48 aact:AA02380|3end_iron_chelatin_ABC_transporter,periplasmic-binding_protein_1629180..1629330_POSITIVE 366 49 4 104 56 aact:AA00523|3end_conserved_hypothetical_protein_358929..359079_POSITIVE 360 47 4 54 13 aact:AA00138|3end_hypothetical_protein_99141..99291_POSITIVE 358 89 55 36 2 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 358 48 9 52 9 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 352 36 12 25 1 aact:AA01889|3end_conserved_hypothetical_protein_(possible_permease)_1274590..1274740_POSITIVE 352 46 6 94 54 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 349 39 1 148 113 aact:AA01582|3end_hypothetical_protein_1065651..1065801_POSITIVE 349 46 2 86 47 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 348 48 3 61 13 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 347 36 1 51 13 aact:AA01796|3end_branched-chain_amino_acid_aminotransferase_1212168..1212318_POSITIVE 346 50 11 92 46 aact:AA01187|3end_conserved_hypothetical_protein_813992..814142_POSITIVE 343 47 1 78 34 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 341 49 2 113 64 aact:AA02253|3end_hypothetical_protein_1534786..1534936_POSITIVE 340 49 9 133 87 aact:AA02074|3end_hypothetical_protein_1409656..1409806_POSITIVE