Origin IGS: ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaggctatcctttttaagtgatatgtcttgtgataacaaagggttatgtttattttatcgttctatgagtacaactcatgtaagaagctgataatgactatagtataatatctcttgtaataagaaatataatttttattgataggtattttaagttactatattttttcgcatactacatagatttatcaaaatgaggtataaaa Mask Tandem Repeat Region ================================================ ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Find is-nt database================================================ Query_seq: AA02856:AA02857|AA02856:AA02857:aspartyl-tRNA synthetase:conserved hypothetical protein:->->:2004600..2004806 207 ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02856:AA02857|AA02856:AA02857:aspartyl-tRNA synthetase:conserved hypothetical protein:->->:2004600..2004806 207 ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02856:AA02857|AA02856:AA02857:aspartyl-tRNA synthetase:conserved hypothetical protein:->->:2004600..2004806 207 ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 13 End: 102 ............ K S L D I Y Y A F F Y L L K F Y R D I F I I N R I V L S I M ......................................................................................................... ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 40 End: 69 .......................................ATTGATAGGTATTTTAAGTTACTATATTTT.......................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02856:AA02857|AA02856:AA02857:aspartyl-tRNA synthetase:conserved hypothetical protein:->->:2004600..2004806 207 ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Intra-Species Hit: Count: 1 Min: 1 Max: 207 Len: 207 Subject: aact_AA02856_AA02857|aspartyl-tRNA synthetase:conserved hypothetical protein|POSITIVE:POSITIVE|[2004600,2004806]|207 HSP 1 e-value: 1.0E-114 bit: 410.0 Len: 207 Query Start:1 Query End:207 Subject Strand: POSITIVE Subject Start: 1 Subject End: 207 ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt ttttatacctcattttgataaatctatgtagtatgcgaaaaaatatagtaacttaaaatacctatcaataaaaattatatttcttattacaagagatattatactatagtcattatcagcttcttacatgagttgtactcatagaacgataaaataaacataaccctttgttatcacaagacatatcacttaaaaaggatagccttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAUACCUCAUUUUGAUAAAUCUAUGUAGUAUGCGAAAAAAUAUAGUAACUUAAAAUACCUAUCAAUAAAAAUUAUAUUUCUUAUUACAAGAGAUAUUAUACUAUAGUCAUUAUCAGCUUCUUACAUGAGUUGUACUCAUAGAACGAUAAAAUAAACAUAACCCUUUGUUAUCACAAGACAUAUCACUUAAAAAGGAUAGCCUUU ...............(((((......(((((((((............(((........)))...............(((((((((.....))))))))))))))))))(((.(((((.(.((((...(((((.....)))))))))))))))....((((........)))).......))).))))).....(((((....))))) (-29.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAGGCUAUCCUUUUUAAGUGAUAUGUCUUGUGAUAACAAAGGGUUAUGUUUAUUUUAUCGUUCUAUGAGUACAACUCAUGUAAGAAGCUGAUAAUGACUAUAGUAUAAUAUCUCUUGUAAUAAGAAAUAUAAUUUUUAUUGAUAGGUAUUUUAAGUUACUAUAUUUUUUCGCAUACUACAUAGAUUUAUCAAAAUGAGGUAUAAAA ......(((((((......(((((.(((((((....)))...(((.((((.....(((((((((((((((.....)))))...))))..))))))....(((((((.(((((((....(((((((((......)))))))))..)))))))......))))))).......)))))))....)))).)))))....)))).)))... (-36.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 129 107 107 85 aact:AA02686|5end_hydrogenase_4_component_C_1871546..1871776_POSITIVE 237 146 109 100 59 aact:AA01448|5end_hypothetical_protein_970208..970438_POSITIVE 237 146 109 43 2 aact:AA01446|5end_conserved_hypothetical_protein_970151..970381_POSITIVE 226 147 109 172 128 aact:AA01177|5end_beta-ketoacyl-ACP_synthase_IV_806732..806962_POSITIVE 219 148 106 193 148 aact:AA02688|5end_hydrogenase_4_component_E_1873977..1874207_POSITIVE 217 153 115 182 142 aact:AA00469|5end_inorganic_pyrophosphatase,_chain_A_326206..326436_POSITIVE 199 124 109 102 87 aact:AA02898|5end_oligopeptide_transport_ATP-binding_protein_2029083..2029313_POSITIVE 198 46 5 100 43 aact:AA01308|5end_cyclic_AMP_receptor_protein_888044..888274_POSITIVE 194 149 103 223 166 aact:AA00972|5end_DNA-binding_protein_HU-ALPHA_677965..678195_POSITIVE 193 123 84 226 176 aact:AA02007|5end_hypothetical_protein_1365748..1365978_POSITIVE 187 148 111 83 44 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 187 141 109 164 134 aact:AA01233|5end_50S_ribosomal_protein_L6_840853..841083_POSITIVE 184 198 164 185 154 aact:AA02111|5end_killing_protein_suppressor_1436877..1437107_POSITIVE 183 149 115 211 183 aact:AA02060|5end_cardiolipin_synthase_1401956..1402186_POSITIVE 182 148 109 231 190 aact:AA00847|5end_S-adenosyl-dependent_methyltransferase_584960..585190_POSITIVE 180 190 141 172 121 aact:AA01169|5end_hypothetical_protein_801985..802215_POSITIVE 180 148 109 223 188 aact:AA00351|5end_50S_ribosomal_protein_L7/L12_234155..234385_POSITIVE 180 148 109 219 184 aact:AA00353|5end_hypothetical_protein_234151..234381_POSITIVE 178 137 113 173 148 aact:AA01774|5end_5,10-methylenetetrahydrofolate_reductase_1195015..1195245_POSITIVE 178 137 113 35 10 aact:AA01773|5end_hypothetical_protein_1194877..1195107_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 129 107 93 71 aact:AA02684|3end_hydrogenase_4_membrane_subunit_1871612..1871762_POSITIVE 237 146 109 138 97 aact:AA01446|3end_conserved_hypothetical_protein_970326..970476_POSITIVE 199 124 109 89 74 aact:AA02897|3end_oligopeptide_ABC_transporter;_permease_protein_2029150..2029300_POSITIVE 187 141 109 145 115 aact:AA01232|3end_30S_ribosomal_protein_S8_840914..841064_POSITIVE 183 149 115 146 118 aact:AA02057|3end_phosphoglycerate_mutase_1401971..1402121_POSITIVE 180 190 141 125 74 aact:AA01168|3end_6-phosphogluconolactonase_802018..802168_POSITIVE 176 190 143 140 100 aact:AA02454|3end_ATP-dependent_helicase_1694433..1694583_POSITIVE 175 148 104 127 89 aact:AA02472|3end_conserved_hypothetical_protein_1708409..1708559_POSITIVE 173 123 82 150 106 aact:AA00505|3end_hypothetical_protein_351733..351883_POSITIVE 171 139 93 135 82 aact:AA00667|3end_cytolethal_distending_toxin_protein_C_458580..458730_POSITIVE 171 149 107 118 77 aact:AA00347|3end_50S_ribosomal_protein_L11_232642..232792_POSITIVE 170 157 118 58 22 aact:AA03003|3end_GTP-binding_membrane_protein_2098447..2098597_POSITIVE 170 134 109 54 31 aact:AA01892|3end_hypothetical_protein_1276896..1277046_POSITIVE 169 148 104 106 70 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 169 124 81 65 25 aact:AA01633|3end_probable_ferredoxin-like_protein_1104333..1104483_POSITIVE 169 72 36 76 30 aact:AA00202|3end_conserved_hypothetical_protein_(possible_membrane_protein)_141420..141570_POSITIVE 167 154 117 43 8 aact:AA02395|3end_ATP-dependent_proteinase_1647107..1647257_POSITIVE 167 120 84 107 71 aact:AA00143|3end_gluconate_permease_(GntP_family)_103796..103946_POSITIVE 166 181 141 50 19 aact:AA01599|3end_phosphocarrier_protein_HPr_1080444..1080594_POSITIVE 165 174 147 113 85 aact:AA01051|3end_iron_(III)_ABC_transporter,_ATP-binding_protein_733172..733322_POSITIVE